Fixation and fate of C and N in the cyanobacterium Trichodesmium using nanometer-scale secondary ion mass spectrometry.

abstract：:In vitro DNA synthesis was studied in HeLa cell nuclei, with emphasis on the question of whether newly replicated DNA is associated with nucleosomes. The newly replicated DNA was twice as sensitive to digestion by micrococcal nuclease as mature chromatin DNA, reaching a limit digest at 20-25% acid-insoluble product. E...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Seale RL

更新日期：1978-06-01 00:00:00

abstract：:X-linked dystonia-parkinsonism (XDP) is a neurodegenerative disease associated with an antisense insertion of a SINE-VNTR-Alu (SVA)-type retrotransposon within an intron of TAF1 This unique insertion coincides with six additional noncoding sequence changes in TAF1, the gene that encodes TATA-binding protein-associated...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bragg DC,Mangkalaphiban K,Vaine CA,Kulkarni NJ,Shin D,Yadav R,Dhakal J,Ton ML,Cheng A,Russo CT,Ang M,Acuña P,Go C,Franceour TN,Multhaupt-Buell T,Ito N,Müller U,Hendriks WT,Breakefield XO,Sharma N,Ozelius LJ

更新日期：2017-12-19 00:00:00

abstract：:Efficient and controllable expression of a transgene usually requires the presence of intron sequences and much efforts have been made to produce retrovirus vectors that can transduce and integrate genes with introns. However, this has proven difficult because the viral RNA is spliced when it is synthesized in the nuc...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Li KJ,Garoff H

更新日期：1998-03-31 00:00:00

abstract：:Developing tools for investigating the cellular activity of glycans will help to delineate the molecular basis for aberrant glycosylation in pathological processes such as cancer. Metabolic oligosaccharide engineering, which inserts sugar-reporting groups into cellular glycoconjugates, represents a powerful method for...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hsu TL,Hanson SR,Kishikawa K,Wang SK,Sawa M,Wong CH

更新日期：2007-02-20 00:00:00

abstract：:Tau is a highly soluble protein, yet it aggregates abnormally in Alzheimer's disease. Here, we address the question of proteolytic processing of tau and the nucleation of aggregates by tau fragments. We show in neuronal cell models that fragments of the repeat domain of tau containing mutations of FTDP17 (frontotempor...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wang YP,Biernat J,Pickhardt M,Mandelkow E,Mandelkow EM

更新日期：2007-06-12 00:00:00

abstract：:We have measured the fidelity of bidirectional, semiconservative DNA synthesis by a human DNA replication complex in vitro. Replication was performed by extracts of HeLa cells in the presence of simian virus 40 (SV40) large tumor antigen by using a double-stranded phage M13mp2 DNA template containing the SV40 origin o...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Roberts JD,Kunkel TA

更新日期：1988-10-01 00:00:00

abstract：:Mechanisms leading to down-regulation of activated microglia and astrocytes are poorly understood, in spite of the potentially detrimental role of activated glia in neurodegeneration. Prostaglandins, produced both by neurons and glia, may serve as mediators of glial and neuronal functions. We examined the influence of...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Petrova TV,Akama KT,Van Eldik LJ

更新日期：1999-04-13 00:00:00

abstract：:We have used X-ray diffraction on the rhombohedral phospholipid phase to reconstruct stalk structures in different pure lipids and lipid mixtures with unprecedented resolution, enabling a quantitative analysis of geometry, as well as curvature and hydration energies. Electron density isosurfaces are used to study shap...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Aeffner S,Reusch T,Weinhausen B,Salditt T

更新日期：2012-06-19 00:00:00

abstract：:The impact of projected global warming on crop yields has been evaluated by indirect methods using simulation models. Direct studies on the effects of observed climate change on crop growth and yield could provide more accurate information for assessing the impact of climate change on crop production. We analyzed weat...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Peng S,Huang J,Sheehy JE,Laza RC,Visperas RM,Zhong X,Centeno GS,Khush GS,Cassman KG

更新日期：2004-07-06 00:00:00

abstract：:Cyanobacterial clock proteins KaiA and KaiC are proposed as positive and negative regulators in the autoregulatory circadian kaiBC expression, respectively. Here, we show that activation of kaiBC expression by kaiA requires KaiC, suggesting a positive feedback control in the cyanobacterial clockwork. We found that rob...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Iwasaki H,Nishiwaki T,Kitayama Y,Nakajima M,Kondo T

更新日期：2002-11-26 00:00:00

abstract：:This study describes a unique function of taurocholate in bile canalicular formation involving signaling through a cAMP-Epac-MEK-Rap1-LKB1-AMPK pathway. In rat hepatocyte sandwich cultures, polarization was manifested by sequential progression of bile canaliculi from small structures to a fully branched network. Tauro...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Fu D,Wakabayashi Y,Lippincott-Schwartz J,Arias IM

更新日期：2011-01-25 00:00:00

abstract：:Disruption of latent Epstein-Barr virus (EBV) infection is induced by the key immediate-early protein BZLF1 (or Z, a member of the basic leucine-zipper family), which transactivates the viral early promoters. Viral reactivation is marked by renewed synthesis of early gene products such as EBV early antigen-diffuse (EA...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sista ND,Pagano JS,Liao W,Kenney S

更新日期：1993-05-01 00:00:00

abstract：:The exemplary search capabilities of flying insects have established them as one of the most diverse taxa on Earth. However, we still lack the fundamental ability to quantify, represent, and predict trajectories under natural contexts to understand search and its applications. For example, flying insects have evolved ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kaushik PK,Renz M,Olsson SB

更新日期：2020-06-02 00:00:00

abstract：:The ever-increasing global need for potable water requires practical, sustainable approaches for purifying abundant alternative sources such as seawater, high-salinity processed water, or underground reservoirs. Evaporation-based solutions are of particular interest for treating high salinity water, since conventional...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Dongare PD,Alabastri A,Neumann O,Nordlander P,Halas NJ

更新日期：2019-07-02 00:00:00

abstract：:ES cells represent a valuable model for investigating early embryo development and hold promise for future regenerative medicine strategies. The self-renewal of pluripotent mouse ES cells has been shown to require extrinsic stimulation by the bone morphogenetic protein (BMP) and leukemia inhibitory factor signaling pa...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Suzuki A,Raya Á,Kawakami Y,Morita M,Matsui T,Nakashima K,Gage FH,Rodríguez-Esteban C,Izpisúa Belmonte JC

更新日期：2006-07-05 00:00:00

abstract：:Bacteriophage display of antibodies provides a method for the generation of immunological reagents against rare and uncharacterized antigens. To ascertain the usefulness of this approach for the characterization of inner-ear proteins, we produced a bacteriophage-displayed antibody-fragment library directed against pro...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Cyr JL,Hudspeth AJ

更新日期：2000-02-29 00:00:00

abstract：:Many neurodegenerative diseases are characterized by the accumulation of amyloid fibers in the brain, which can occur when a protein misfolds into an extended beta-sheet conformation. The nucleation of these beta-sheet aggregates is of particular interest, not only because it is the rate-determining step toward fiber ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Petty SA,Decatur SM

更新日期：2005-10-04 00:00:00

abstract：:Whole human blood was examined by (31)P nuclear magnetic resonance spectroscopy. Individual phosphates (alpha,beta,gamma) of ATP were identifiable, and two microenvironments appeared to be present for this molecule. When sequential recordings of freshly collected blood were made, 2,3-diphosphoglycerate was observed to...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Henderson TO,Costello AJ,Omachi A

更新日期：1974-06-01 00:00:00

abstract：:Initial attempts to correlate the distribution of gene density (number of gene loci per unit length on the linkage map) with the distribution of lengths of coding sequences have led to the observation that 46% of approximately 1000 sampled proteins in Escherichia coli have molecular masses of n X 14,000 +/- 2500 dalto...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Savageau MA

更新日期：1986-03-01 00:00:00

abstract：:A number of eukaryotic viruses have evolved mechanisms to downregulate activity of the interferon-induced, double-stranded RNA-activated protein kinase (referred to as P68 based on its Mr of 68,000 in human cells). This control is essential because once activated, the P68 kinase phosphorylates its natural substrate, t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lee TG,Tomita J,Hovanessian AG,Katze MG

更新日期：1990-08-01 00:00:00

abstract：:We purified microtubules from a mammalian mitotic extract and obtained an amino acid sequence from each microtubule-associated protein by using mass spectrometry. Most of these proteins are known spindle-associated components with essential functional roles in spindle organization. We generated antibodies against a pr...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mack GJ,Compton DA

更新日期：2001-12-04 00:00:00

abstract：:The murine-virus-infected erythroleukemia cell system provides an opportunity to examine regulatory mechanisms controlling cytodifferentiation. A cloned cell line (DR10c3) resistant to the erythropoiesis-inducing effect of dimethylsulfoxide (Me2SO) was isolated from the Me2SO-sensitive line DS19. DR10c3 is characteriz...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ohta Y,Tanaka M,Terada M,Miller OJ,Bank A,Marks P,Rifkind RA

更新日期：1976-04-01 00:00:00

abstract：:An alpha-bungarotoxin-horseradish peroxidase conjugate, which binds specificity to nicotinic acetylcholine receptors, was synthesized. This conjugate was bound by 5-7% of the synapses in the inner plexiform layer of the chicken retina. Bipolar ribbon synapses as well as amacrine synapses bound the conjugate. ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Vogel Z,Maloney GJ,Ling A,Daniels MP

更新日期：1977-08-01 00:00:00

abstract：:We have long known that language is lateralized to the left hemisphere (LH) in most neurologically healthy adults. In contrast, findings on lateralization of function during development are more complex. As in adults, anatomical, electrophysiological, and neuroimaging studies in infants and children indicate LH latera...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Olulade OA,Seydell-Greenwald A,Chambers CE,Turkeltaub PE,Dromerick AW,Berl MM,Gaillard WD,Newport EL

更新日期：2020-09-22 00:00:00

abstract：:There is an important medical need for new antifungal agents with novel mechanisms of action to treat the increasing number of patients with life-threatening systemic fungal disease and to overcome the growing problem of resistance to current therapies. F901318, the leading representative of a novel class of drug, the...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Oliver JD,Sibley GEM,Beckmann N,Dobb KS,Slater MJ,McEntee L,du Pré S,Livermore J,Bromley MJ,Wiederhold NP,Hope WW,Kennedy AJ,Law D,Birch M

更新日期：2016-11-08 00:00:00

abstract：:Peptides from the three type I repeats of human endothelial cell thrombospondin, containing the consensus sequence-Trp-Ser-Xaa-Trp-, bind to sulfated glycoconjugates including heparin and sulfatide. The peptides are potent inhibitors for the binding of thrombospondin, laminin, or apolipoprotein E to these ligands. The...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Guo NH,Krutzsch HC,Nègre E,Vogel T,Blake DA,Roberts DD

更新日期：1992-04-01 00:00:00

abstract：:Microbial pathogens with the ability to establish chronic infections have evolved strategies to actively modulate the host immune response. Brucellosis is a disease caused by a Gram-negative intracellular pathogen that if not treated during the initial phase of the infection becomes chronic as the bacteria persist for...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Spera JM,Ugalde JE,Mucci J,Comerci DJ,Ugalde RA

更新日期：2006-10-31 00:00:00

abstract：:Breast cancer is a genetically and clinically heterogeneous disease, and the contributions of different target cells and different oncogenic mutations to this heterogeneity are not well understood. Here we report that mammary tumors induced by components of the Wnt signaling pathway contain heterogeneous cell types an...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Li Y,Welm B,Podsypanina K,Huang S,Chamorro M,Zhang X,Rowlands T,Egeblad M,Cowin P,Werb Z,Tan LK,Rosen JM,Varmus HE

更新日期：2003-12-23 00:00:00

abstract：:The sequence of the cloned Bacillus amyloliquefaciens subtilisin gene suggested that this secreted serine protease is produced as a larger precursor, designated preprosubtilisin [Wells, J. A., Ferrari, E., Henner, D. J., Estell, D. A. & Chen, E. Y. (1983) Nucleic Acids Res. 11, 7911-7925]. Biochemical evidence present...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Power SD,Adams RM,Wells JA

更新日期：1986-05-01 00:00:00

abstract：:Photosystem II (PSII) is an intrinsic membrane protein complex that functions as a light-driven water:plastoquinone oxidoreductase in oxygenic photosynthesis. Electron transport in PSII is associated with formation of reactive oxygen species (ROS) responsible for oxidative modifications of PSII proteins. In this study...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kumar A,Prasad A,Sedlářová M,Kale R,Frankel LK,Sallans L,Bricker TM,Pospíšil P

更新日期：2021-01-26 00:00:00