Abstract:
:We have used X-ray diffraction on the rhombohedral phospholipid phase to reconstruct stalk structures in different pure lipids and lipid mixtures with unprecedented resolution, enabling a quantitative analysis of geometry, as well as curvature and hydration energies. Electron density isosurfaces are used to study shape and curvature properties of the bent lipid monolayers. We observe that the stalk structure is highly universal in different lipid systems. The associated curvatures change in a subtle, but systematic fashion upon changes in lipid composition. In addition, we have studied the hydration interaction prior to the transition from the lamellar to the stalk phase. The results indicate that facilitating dehydration is the key to promote stalk formation, which becomes favorable at an approximately constant interbilayer separation of 9.0 ± 0.5 Å for the investigated lipid compositions.
journal_name
Proc Natl Acad Sci U S Aauthors
Aeffner S,Reusch T,Weinhausen B,Salditt Tdoi
10.1073/pnas.1119442109subject
Has Abstractpub_date
2012-06-19 00:00:00pages
E1609-18issue
25eissn
0027-8424issn
1091-6490pii
1119442109journal_volume
109pub_type
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