Catalytic activation of glycosyl phosphates for stereoselective coupling reactions.

abstract：:Expression systems based on the Escherichia coli arabinose operon P(BAD) promoter exhibit the all-or-nothing (autocatalytic) induction of expression that was first documented in the lac operon. Under conditions of subsaturating levels of inducer, some of the cells of the population are fully induced, whereas other cel...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Morgan-Kiss RM,Wadler C,Cronan JE Jr

更新日期：2002-05-28 00:00:00

abstract：:Mouse teratocarcinoma cells induced to differentiate in vitro undergo a massive (30%) demethylation of DNA. A similar undermethylation is also observed in the mouse extraembryonic membranes, the yolk sac and placenta. In both cases, the decrease in methyl moieties occurs at a large number of CpG sites spread out over ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Razin A,Webb C,Szyf M,Yisraeli J,Rosenthal A,Naveh-Many T,Sciaky-Gallili N,Cedar H

更新日期：1984-04-01 00:00:00

abstract：:Several proteins have been identified that protect Drosophila telomeres from fusion events. They include UbcD1, HP1, HOAP, the components of the Mre11-Rad50-Nbs (MRN) complex, the ATM kinase, and the putative transcription factor Woc. Of these proteins, only HOAP has been shown to localize specifically at telomeres. H...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Raffa GD,Siriaco G,Cugusi S,Ciapponi L,Cenci G,Wojcik E,Gatti M

更新日期：2009-02-17 00:00:00

abstract：:The transforming growth factor-beta (TGF-beta) superfamily of growth factors and cytokines has been implicated in a variety of physiological and developmental processes within the cardiovascular system. Smad proteins are a recently described family of intracellular signaling proteins that transduce signals in response...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Topper JN,DiChiara MR,Brown JD,Williams AJ,Falb D,Collins T,Gimbrone MA Jr

更新日期：1998-08-04 00:00:00

abstract：:The E2F1 transcription factor has a well-characterized activation domain at its C terminus and the E1A protein has a recently defined activation domain at its N terminus. Here we show that these activation domains are highly related in sequence. The sequence homology reflects, at least partly, the conservation of comm...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Trouche D,Kouzarides T

更新日期：1996-02-20 00:00:00

abstract：:One of the greatest challenges in contemporary condensed matter physics is to ascertain whether the formation of glasses from liquids is fundamentally thermodynamic or dynamic in origin. Although the thermodynamic paradigm has dominated theoretical research for decades, the purely kinetic perspective of the dynamical ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mishra CK,Hima Nagamanasa K,Ganapathy R,Sood AK,Gokhale S

更新日期：2014-10-28 00:00:00

abstract：:When either the homologous RNA (avian myeloblastosis virus RNA) or a heterologous RNA (poliovirus RNA) was used as a template, the anticomplementary DNA synthesized in vitro by avian myeloblastosis virus reverse transcriptase (RNA-directed DNA nucleotidyltransferase, EC 2.7.7.7) was primed by fragments of the original...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Myers JC,Dobkin C,Spiegelman S

更新日期：1980-03-01 00:00:00

abstract：:Atherosclerosis is associated with alterations in nitric oxide (NO)/cGMP signaling. In early stages of the disease, inflammatory and possibly other cells produce reactive oxygen species that scavenge vasoprotective NO. In addition to the oxidative stress, expression and activity of enzymes downstream to NO formation m...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Melichar VO,Behr-Roussel D,Zabel U,Uttenthal LO,Rodrigo J,Rupin A,Verbeuren TJ,Kumar H S A,Schmidt HH

更新日期：2004-11-23 00:00:00

abstract：:Epidermal growth factor (EGF), a mitogen, also stimulates neurite extension during development, but the underlying mechanism is elusive. This study reveals a functional role for kappa opioid receptor (KOR) in EGF-stimulated neurite extension, and the underlying mechanism. EGF and activated EGF receptor (EGFR) levels a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tsai NP,Tsui YC,Pintar JE,Loh HH,Wei LN

更新日期：2010-02-16 00:00:00

abstract：:TNF superfamily death ligands are expressed on the surface of immune cells and can trigger apoptosis in susceptible cancer cells by engaging cognate death receptors. A recombinant soluble protein comprising the ectodomain of Apo2 ligand/TNF-related apoptosis-inducing ligand (Apo2L/TRAIL) has shown remarkable preclinic...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Nair PM,Flores H,Gogineni A,Marsters S,Lawrence DA,Kelley RF,Ngu H,Sagolla M,Komuves L,Bourgon R,Settleman J,Ashkenazi A

更新日期：2015-05-05 00:00:00

abstract：:Oncogene-induced senescence (OIS) is a critical tumor-suppressing mechanism that restrains cancer progression at premalignant stages, in part by causing telomere dysfunction. Currently it is unknown whether this proliferative arrest presents a stable and therefore irreversible barrier to cancer progression. Here we de...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Patel PL,Suram A,Mirani N,Bischof O,Herbig U

更新日期：2016-08-23 00:00:00

abstract：:DNA toroids produced by the condensation of lambda phage DNA with hexammine cobalt (III) have been investigated by cryoelectron microscopy. Image resolution obtained by this technique has allowed unprecedented views of DNA packing within toroidal condensates. Toroids oriented coplanar with the microscope image plane e...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hud NV,Downing KH

更新日期：2001-12-18 00:00:00

abstract：:The prefrontal cortex continues to mature after puberty and into early adulthood, mirroring the time course of maturation of cognitive abilities. However, the way in which prefrontal activity changes during peri- and postpubertal cortical maturation is largely unknown. To address this question, we evaluated the develo...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zhou X,Zhu D,Katsuki F,Qi XL,Lees CJ,Bennett AJ,Salinas E,Stanford TR,Constantinidis C

更新日期：2014-03-11 00:00:00

abstract：:Shoot architecture is critical for optimizing plant adaptation and productivity. In contrast with annuals, branching in perennials native to temperate and boreal regions must be coordinated with seasonal growth cycles. How branching is coordinated with seasonal growth is poorly understood. We identified key components...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Maurya JP,Miskolczi PC,Mishra S,Singh RK,Bhalerao RP

更新日期：2020-05-26 00:00:00

abstract：:When Drosophila melanogaster embryos were ligated at very early stages of nuclear multiplication (45-60 min after egg deposition) for the entire period of embryogenesis, egg fragments of variable sizes were able to continue development up to the hatching stage. These ligated embryos differentiated larval structures; a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Schubiger G,Moseley RC,Wood WJ

更新日期：1977-05-01 00:00:00

abstract：:Tentoxin, a natural cyclic tetrapeptide produced by phytopathogenic fungi from the Alternaria species affects the catalytic function of the chloroplast F(1)-ATPase in certain sensitive species of plants. In this study, we show that the uncompetitive inhibitor tentoxin binds to the alphabeta-interface of the chloroplas...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Groth G

更新日期：2002-03-19 00:00:00

abstract：:Phospholipids containing photolysable carbene precursors (beta-trifluoro-alpha-diazopropionoxy and m-diazirinophenoxy groups) in omega-positions of sn-2 fatty acyl chains were prepared. Photolysis of their vesicles produced crosslinked products in 40-60% yields. Crosslinking was mostly intermolecular and occurred by c...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gupta CM,Radhakrishnan R,Gerber GE,Olsen WL,Quay SC,Khorana HG

更新日期：1979-06-01 00:00:00

abstract：:The tumor suppressor Smad4/Dpc4 is a transcription activator that binds specific DNA sequences and whose nuclear localization is induced after exposure to type beta transforming growth factor-like cytokines. We explored an inducible system in which Smad4 protein is activated by translocation to the nucleus when cell l...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Dai JL,Bansal RK,Kern SE

更新日期：1999-02-16 00:00:00

abstract：:Somatic hypermutation (SHM) and class-switch recombination (CSR) of the Ig gene require both the transcription of the locus and the expression of activation-induced cytidine deaminase (AID). During CSR, AID decreases the amount of topoisomerase I (Top1); this decrease alters the DNA structure and induces cleavage in t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kobayashi M,Sabouri Z,Sabouri S,Kitawaki Y,Pommier Y,Abe T,Kiyonari H,Honjo T

更新日期：2011-11-29 00:00:00

abstract：:The PsaF-deficient mutant 3bF of Chlamydomonas reinhardtii was used to modify PsaF by nuclear transformation and site-directed mutagenesis. Four lysine residues in the N-terminal domain of PsaF, which have been postulated to form the positively charged face of a putative amphipathic alpha-helical structure were altere...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hippler M,Drepper F,Haehnel W,Rochaix JD

更新日期：1998-06-23 00:00:00

abstract：:Partial amino acid sequence has been determined for the cone, alpha' subunit of the bovine photoreceptor cyclic nucleotide phosphodiesterase (PDE) and deduced from nucleotide sequences of a partial cDNA clone. These sequences identify the alpha' subunit as the product of a gene that is distinct from those encoding the...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Charbonneau H,Prusti RK,LeTrong H,Sonnenburg WK,Mullaney PJ,Walsh KA,Beavo JA

更新日期：1990-01-01 00:00:00

abstract：:To study the origin of and the degree of T-cell antigen receptor (TCR) diversity of Thy-1+ dendritic epidermal cells (Thy-1+ dECs) in mice, we have developed a monoclonal antibody (mAb 536) to the gamma delta TCR. mAb 536 binds to and stimulates interleukin 2 secretion from Thy-1+ dEC but not cells that express TCR co...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Havran WL,Grell S,Duwe G,Kimura J,Wilson A,Kruisbeek AM,O'Brien RL,Born W,Tigelaar RE,Allison JP

更新日期：1989-06-01 00:00:00

abstract：:The crystal structure of raite was solved and refined from data collected at Beamline Insertion Device 13 at the European Synchrotron Radiation Facility, using a 3 x 3 x 65 microm single crystal. The refined lattice constants of the monoclinic unit cell are a = 15.1(1) A; b = 17.6(1) A; c = 5.290(4) A; beta = 100.5(2)...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Pluth JJ,Smith JV,Pushcharovsky DY,Semenov EI,Bram A,Riekel C,Weber HP,Broach RW

更新日期：1997-11-11 00:00:00

abstract：:The reproductive activity of Trypanosoma lewisi was strikingly enhanced and sometimes prolonged for 2 days when sublethal doses of adenine were given to rats. The drug was effective when given for 5 or 7 days in the diet and/or intraperitoneally, starting one or two days before infection through two days after infecti...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Taliaferro WH,D'Alesandro PA

更新日期：1971-01-01 00:00:00

abstract：:Recent advances in understanding the role of neurotrophins on activity-dependent plasticity have provided insight into how behavior can affect specific aspects of neuronal biology. We present evidence that voluntary exercise can prime adult dorsal root ganglion neurons for increased axonal regeneration through a neuro...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Molteni R,Zheng JQ,Ying Z,Gómez-Pinilla F,Twiss JL

更新日期：2004-06-01 00:00:00

abstract：:We describe a physical map of the end of the long arm of the human X chromosome encompassing the region from Xq27.2 to the q telomere, inclusive of the chromosomal band Xq28. This region is of particular interest, since it contains the highest density of genes associated with genetic diseases. The map covers a total o...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Poustka A,Dietrich A,Langenstein G,Toniolo D,Warren ST,Lehrach H

更新日期：1991-10-01 00:00:00

abstract：:The application of boron neutron capture therapy to rheumatoid arthritis requires the selective delivery of the boron-10 isotope to the synovitic tissue. The use of liposomes as a boron delivery method has been explored through the measurement of the time course biodistribution of boron in rats with collagen-induced a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Watson-Clark RA,Banquerigo ML,Shelly K,Hawthorne MF,Brahn E

更新日期：1998-03-03 00:00:00

abstract：:For over 40 y the dominant theory of stomatal behavior has been that plants should open stomates until the carbon gained by an infinitesimal additional opening balances the additional water lost times a water price that is constant at least over short periods. This theory has persisted because of its remarkable succes...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wolf A,Anderegg WR,Pacala SW

更新日期：2016-11-15 00:00:00

abstract：:Coacervation is a common phenomenon in natural polymers and has been applied to synthetic materials systems for coatings, adhesives, and encapsulants. Single-component coacervates are formed when block polyampholytes exhibit self-coacervation, phase separating into a dense liquid coacervate phase rich in the polyampho...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Danielsen SPO,McCarty J,Shea JE,Delaney KT,Fredrickson GH

更新日期：2019-04-23 00:00:00

abstract：:Activation of protein kinase C (PKC) by phorbol ester facilitates hormonal secretion and transmitter release, and phorbol ester-induced synaptic potentiation (PESP) is a model for presynaptic facilitation. A variety of PKC isoforms are expressed in the central nervous system, but the isoform involved in the PESP has n...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Saitoh N,Hori T,Takahashi T

更新日期：2001-11-20 00:00:00