Abstract:
:High-affinity antibodies are critical for host protection and underlie successful vaccines. The generation of such antibodies requires T cell-dependent help, which mediates germinal center reactions in which mutation and selection of B cells occurs. Using an interleukin 4-reporter system, we show here that CD4(+) follicular helper T cells constituted essentially all of the cytokine-secreting T cells in lymph nodes and were functionally distinct from T cells secreting the same cytokine in peripheral tissues. Follicular helper T cells with different cytokine profiles could be isolated as conjugates with B cells undergoing cytokine-specific immunoglobulin class switching with evidence of somatic hypermutation. Our findings support a model in which B cells compete for cytokines produced by follicular helper T cells that shape the affinity and isotype of the antibody response.
journal_name
Nat Immunoljournal_title
Nature immunologyauthors
Reinhardt RL,Liang HE,Locksley RMdoi
10.1038/ni.1715subject
Has Abstractpub_date
2009-04-01 00:00:00pages
385-93issue
4eissn
1529-2908issn
1529-2916pii
ni.1715journal_volume
10pub_type
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