Abstract：
:Brief cerebral ischemia leads to selective neuronal necrosis (SNN), which is characterized by neuronal death with sparing of glial and vascular elements of the central nervous system. Understanding the pathophysiology of SNN may help elucidating the mechanisms and consequences of neuronal injury in humans following brief ischemia. Contrary to the presence of reproducible models of transient global ischemia, animal models of transient focal ischemia producing SNN are scarce and have important limitations such as causing ischemia in a vast area and inducing additional insults. In this study, we developed a practical mouse model of SNN without these limitations, by compressing the distal middle cerebral artery (MCA) with a blunted micropipette for 15 min. The success of compression was evaluated by monitoring the regional cerebral blood flow, and conventional histopathology and immunolabeling of the brain sections. Seven/fourteen days after ischemia, intracisternally administered propidium iodide labeled numerous necrotic cells in the frontoparietal cortex, which were mostly NeuN-positive, but were not immunolabeled with astrocytic markers (GFAP and S100), and showed neuronal morphology with hematoxylin-eosin staining, indicating that the model successfully induced ischemic injury limited to neurons. The model could become an important tool for investigating the long-term effects of brief ischemic events like transient ischemic attacks and could offer convenient reversible distal MCA occlusion for studies using intravital microscopy.
journal_name
Brain Res Bulljournal_title
Brain research bulletinauthors
Arsava EM,Gurer G,Gursoy-Ozdemir Y,Karatas H,Dalkara Tdoi
10.1016/j.brainresbull.2008.11.005subject
Has Abstractpub_date
2009-03-16 00:00:00pages
226-31issue
4-5eissn
0361-9230issn
1873-2747pii
S0361-9230(08)00378-Xjournal_volume
78pub_type
杂志文章abstract：:We have studied the effects of 14-methoxymetopon (HS 198), a potent opioid agonist, on the responses to the tail electric stimulation test and plus-maze activity of adult male rats. The prototype mu agonist morphine was used as the drug of reference. Besides we addressed the effects of HS 198 on the serum corticostero...
journal_title：Brain research bulletin
pub_type： 杂志文章
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journal_title：Brain research bulletin
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journal_title：Brain research bulletin
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pub_type： 杂志文章
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pub_type： 杂志文章
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journal_title：Brain research bulletin
pub_type： 杂志文章
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pub_type： 杂志文章
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pub_type： 杂志文章
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journal_title：Brain research bulletin
pub_type： 杂志文章
doi：10.1016/0361-9230(91)90125-4
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pub_type： 杂志文章
doi：10.1016/j.brainresbull.2005.02.028
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journal_title：Brain research bulletin
pub_type： 杂志文章
doi：10.1016/0361-9230(83)90097-7
更新日期：1983-03-01 00:00:00
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pub_type： 杂志文章
doi：10.1016/j.brainresbull.2020.05.016
更新日期：2020-09-01 00:00:00
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journal_title：Brain research bulletin
pub_type： 杂志文章
doi：10.1016/0361-9230(85)90008-5
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journal_title：Brain research bulletin
pub_type： 杂志文章
doi：10.1016/0361-9230(95)00008-3
更新日期：1995-01-01 00:00:00
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journal_title：Brain research bulletin
pub_type： 杂志文章
doi：10.1016/j.brainresbull.2020.07.011
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pub_type： 杂志文章
doi：10.1016/s0361-9230(03)00042-x
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journal_title：Brain research bulletin
pub_type： 杂志文章
doi：10.1016/0361-9230(76)90081-2
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pub_type： 杂志文章
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pub_type： 杂志文章,评审
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journal_title：Brain research bulletin
pub_type： 杂志文章,评审
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journal_title：Brain research bulletin
pub_type： 杂志文章
doi：10.1016/0361-9230(88)90114-1
更新日期：1988-07-01 00:00:00
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journal_title：Brain research bulletin
pub_type： 杂志文章
doi：10.1016/s0361-9230(98)00015-x
更新日期：1998-06-01 00:00:00
abstract：:Homocysteine-inducible endoplasmic reticulum stress-inducible ubiquitin-like domain member 1 protein (HERPUD1) is involved in endoplasmic reticulum stress response. Immense amounts of research showed HERPUD1 plays multiple roles in various models. In this work, we explored the role of HERPUD1 during the pathophysiolog...
journal_title：Brain research bulletin
pub_type： 杂志文章
doi：10.1016/j.brainresbull.2016.11.006
更新日期：2017-01-01 00:00:00
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journal_title：Brain research bulletin
pub_type： 杂志文章
doi：10.1016/j.brainresbull.2020.05.017
更新日期：2020-09-01 00:00:00
abstract：:Administration of methionine enkephalin (ICV) to rats for 5 days resulted in the development of physical dependence as exemplified by a hypothermic response which peaked 2-8 hours after initiation of withdrawal. Twenty-four hours post-withdrawal, opioid receptor binding was determined in the striatum using a selective...
journal_title：Brain research bulletin
pub_type： 杂志文章
doi：10.1016/0361-9230(86)90123-1
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abstract：:It is known that spinal nociceptive sensory transmission receives descending inhibitory and facilitatory modulation from supraspinal structures. Glutamate is the major fast excitatory transmitter between primary afferent fibers and spinal dorsal horn neurons. In whole-cell patch clamp recordings from dorsal horn neuro...
journal_title：Brain research bulletin
pub_type： 杂志文章
doi：10.1016/s0361-9230(01)00470-1
更新日期：2001-04-01 00:00:00
abstract：:Effects of acute exposure (2 hr) to either 1.5% halothane or 0.5% methoxyflurane were investigated in the Sprague Dawley rat. Pituitary (PIT) and central nervous system (CNS) substance P (SP)-like and beta-endorphin (beta-end)-like immunoreactivities were evaluated immediately after anesthetic exposure (2 h), after ri...
journal_title：Brain research bulletin
pub_type： 杂志文章
doi：10.1016/s0361-9230(97)00436-x
更新日期：1998-03-15 00:00:00
abstract：:The chronic mild stress (CMS) model has been used as an animal model of depression which induces anhedonic behavior in rodents. The present study was aimed to evaluate the behavioral and physiological effects of administration of beta-carboline harmine in rats exposed to CMS procedure. To this aim, after 40 days of ex...
journal_title：Brain research bulletin
pub_type： 杂志文章
doi：10.1016/j.brainresbull.2009.09.008
更新日期：2010-03-16 00:00:00
