Abstract:
:LC3 is a widely used marker of autophagosomes in mammalian cells. However, in addition to its autophagosomal localization, GFP-LC3 is often found associated with protein aggregates that are formed in an autophagy-independent manner. In addition, LC3 directly interacts with p62/SQSTM1 (hereafter named p62), a common constituent of protein aggregates. In our recent report, we mapped the regions in LC3 involved in its binding to p62 and showed that this binding is essential for the incorporation of p62 into autophagosomes. Here we demonstrate that the autophagy-unrelated association of GFP-LC3 with protein aggregates is dependent on its interaction with p62.
journal_name
Autophagyjournal_title
Autophagyauthors
Shvets E,Elazar Zdoi
10.4161/auto.6823subject
Has Abstractpub_date
2008-11-01 00:00:00pages
1054-6issue
8eissn
1554-8627issn
1554-8635pii
6823journal_volume
4pub_type
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