Abstract:
:Autophagy plays critical roles in plant responses to stress. In contrast to the wealth of information concerning the core process of plant autophagosome assembly, our understanding of the regulation of autophagy is limited. In this study, we demonstrated that transcription factor HsfA1a played a critical role in tomato tolerance to drought stress, in part through its positive role in induction of autophagy under drought stress. HsfA1a expression was induced by drought stress. Virus-induced HsfA1a gene silencing reduced while its overexpression increased plant drought tolerance based on both symptoms and membrane integrity. HsfA1a-silenced plants were more sensitive to endogenous ABA-mediated stomatal closure, while its overexpression lines were resistant under drought stress, indicating that phytohormone ABA did not play a major role in HsfA1a-induced drought tolerance. On the other hand, HsfA1a-silenced plants increased while its overexpression decreased the levels of insoluble proteins which were highly ubiquitinated under drought stress. Furthermore, drought stress induced numerous ATGs expression and autophagosome formation in wild-type plants. The expression of ATG10 and ATG18f, and the formation of autophagosomes were compromised in HsfA1a-silenced plants but were enhanced in HsfA1a-overexpressing plants. Both electrophoretic mobility shift assay and chromatin immunoprecipitation coupled with qPCR analysis revealed that HsfA1a bound to ATG10 and ATG18f gene promoters. Silencing of ATG10 and ATG18f reduced HsfA1a-induced drought tolerance and autophagosome formation in plants overexpressing HsfA1a. These results demonstrate that HsfA1a induces drought tolerance by activating ATG genes and inducing autophagy, which may promote plant survival by degrading ubiquitinated protein aggregates under drought stress.
journal_name
Autophagyjournal_title
Autophagyauthors
Wang Y,Cai S,Yin L,Shi K,Xia X,Zhou Y,Yu J,Zhou Jdoi
10.1080/15548627.2015.1098798subject
Has Abstractpub_date
2015-11-02 00:00:00pages
2033-2047issue
11eissn
1554-8627issn
1554-8635journal_volume
11pub_type
杂志文章相关文献
Autophagy文献大全abstract::The Atg1/ULK1 complex plays a central role in starvation-induced autophagy, integrating signals from upstream sensors such as MTOR and AMPK and transducing them to the downstream autophagy pathway. Much progress has been made in the last few years in understanding the mechanisms by which the complex is regulated throu...
journal_title:Autophagy
pub_type: 杂志文章,评审
doi:10.4161/auto.23323
更新日期:2013-02-01 00:00:00
abstract::Nutrients not only act as building blocks but also as signaling molecules. Nutrient-availability promotes cell growth and proliferation and suppresses catabolic processes, such as macroautophagy/autophagy. These effects are mediated by checkpoint kinases such as MTOR (mechanistic target of rapamycin kinase), which is ...
journal_title:Autophagy
pub_type: 杂志文章
doi:10.1080/15548627.2020.1783119
更新日期:2020-07-05 00:00:00
abstract::Phosphatidylinositol-3-phosphate (PtdIns3P) is essential for generating autophagosomes and regulating endocytic trafficking. Recently, we have shown that the activities of human PIK3C3/VPS34-containing complexes I and II, which synthesize PtdIns3P, are greatly affected by three membrane physicochemical parameters: lip...
journal_title:Autophagy
pub_type: 杂志文章
doi:10.1080/15548627.2021.1872190
更新日期:2021-01-15 00:00:00
abstract::The uptake and degradation of cytoplasmic material by vacuolar autophagy in plants has been studied extensively by electron microscopy and shown to be involved in developmental processes such as vacuole formation, deposition of seed storage proteins and senescence, and in the response of plants to nutrient starvation ...
journal_title:Autophagy
pub_type: 杂志文章,评审
doi:10.4161/auto.2092
更新日期:2006-01-01 00:00:00
abstract::Neurons are highly polarized cells composed of two distinct domains, the axon and the somatodendritic domain. Although AMPA-type glutamate receptors, which mediate fast excitatory neurotransmission in the vertebrate CNS, are preferentially expressed in the somatodendritic domain, the molecular mechanisms underlying su...
journal_title:Autophagy
pub_type: 杂志文章
doi:10.4161/auto.6493
更新日期:2008-08-01 00:00:00
abstract::Autophagy is a degradation pathway for the turnover of dysfunctional organelles or aggregated proteins in cells. Extracellular accumulation of beta-amyloid peptide has been reported to be a major cause of Alzheimer disease (AD) and large numbers of autophagic vacuoles accumulate in the brain of AD patient. However, ho...
journal_title:Autophagy
pub_type: 杂志文章
doi:10.4161/auto.5.4.8096
更新日期:2009-05-01 00:00:00
abstract::Phosphoinositides (PIs) are a group of low-abundance phospholipids that play a critical role in the control of organelle and membrane traffic. There is strong evidence that specific PIs are also important for the regulation of autophagy. PIs are modified by a complex network of lipid kinases and phosphatases. A recent...
journal_title:Autophagy
pub_type: 杂志文章
doi:10.4161/auto.6.6.12624
更新日期:2010-08-01 00:00:00
abstract::Macroautophagy sequesters superflous cytosol and organelles into double-membraned autophagosomes. Over 30 autophagy-related (ATG) genes have been identified without elucidating the molecular details of autophagosome biogenesis. All proposed models for autophagosome formation require membrane fusion events (Fig. 1). Pr...
journal_title:Autophagy
pub_type: 杂志文章
doi:10.4161/auto.7.2.14383
更新日期:2011-02-01 00:00:00
abstract::BBC3 (BCL2 binding component 3) is a known apoptosis inducer; however, its role in microglial survival remains poorly understood. In addition to the classical transcription factor TRP53, Mir143 is involved in BBC3 expression at the post-transcriptional level. Here, we identify unique roles of Mir143-BBC3 in mediating ...
journal_title:Autophagy
pub_type: 杂志文章
doi:10.1080/15548627.2016.1191723
更新日期:2016-09-01 00:00:00
abstract::(Macro)autophagy is a membrane-trafficking process that serves to sequester cellular constituents in organelles termed autophagosomes, which target their degradation in the lysosome. Autophagy operates at basal levels in all cells where it serves as a homeostatic mechanism to maintain cellular integrity. The levels an...
journal_title:Autophagy
pub_type: 杂志文章
doi:10.4161/auto.7.11.16646
更新日期:2011-11-01 00:00:00
abstract::Aged and damaged mitochondria can be selectively degraded by specific autophagic elimination, termed mitophagy. Defects in mitophagy have been increasingly linked to several diseases including neurodegenerative diseases, metabolic diseases and other aging-related diseases. However, the molecular mechanisms of mitophag...
journal_title:Autophagy
pub_type: 杂志文章
doi:10.1080/15548627.2018.1501251
更新日期:2018-01-01 00:00:00
abstract::Autophagy is a lysosome-mediated catabolic process involving the degradation of intracellular contents (e.g., proteins and organelles) as well as invading microbes (e.g., parasites, bacteria and viruses). Multiple forms of cellular stress can stimulate this pathway, including nutritional imbalances, oxygen deprivation...
journal_title:Autophagy
pub_type: 杂志文章,评审
doi:10.4161/auto.23691
更新日期:2013-04-01 00:00:00
abstract::In yeast, Tom22, the central component of the TOMM (translocase of outer mitochondrial membrane) receptor complex, is responsible for the recognition and translocation of synthesized mitochondrial precursor proteins, and its protein kinase CK2-dependent phosphorylation is mandatory for TOMM complex biogenesis and prop...
journal_title:Autophagy
pub_type: 杂志文章
doi:10.1080/15548627.2017.1403716
更新日期:2018-01-01 00:00:00
abstract::Survival of environmental stress conditions requires the maintenance of cellular homeostasis. To preserve this balance, cells utilize a degradative mechanism known as autophagy. During this process, in response to starvation or other stresses, bulk cytoplasm is non-specifically sequestered within double-membrane vesic...
journal_title:Autophagy
pub_type: 杂志文章
doi:10.4161/auto.4129
更新日期:2007-09-01 00:00:00
abstract::Autophagy is a highly conserved homeostatic pathway that plays an important role in tumor development and progression by acting on cancer cells in a cell-autonomous mechanism. However, the solid tumor is not an island, but rather an ensemble performance that includes nonmalignant stromal cells, such as macrophages. A ...
journal_title:Autophagy
pub_type: 杂志文章,评审
doi:10.4161/auto.26927
更新日期:2014-02-01 00:00:00
abstract::Adaptive immunity, which plays an important role in the development of atherosclerosis, is mediated by major histocompatibility complex (MHC)-dependent antigen presentation. In atherosclerotic lesions, macrophages constitute an important class of antigen-presenting cells that activate adaptive immune responses to oxid...
journal_title:Autophagy
pub_type: 杂志文章
doi:10.1080/15548627.2015.1037061
更新日期:2015-01-01 00:00:00
abstract::Formation of metastases, also known as cancer dissemination, is an important stage of breast cancer (BrCa) development. KISS1 expression is associated with inhibition of metastases development. Recently we have demonstrated that BrCa metastases to the brain exhibit low levels of KISS1 expression at both mRNA and prote...
journal_title:Autophagy
pub_type: 杂志文章
doi:10.1080/15548627.2017.1360466
更新日期:2017-01-01 00:00:00
abstract::The extensive autophagic-lysosomal pathology in Alzheimer disease (AD) brain has revealed a major defect: in the proteolytic clearance of autophagy substrates. Autophagy failure contributes on several levels to AD pathogenesis and has become an important therapeutic target for AD and other neurodegenerative diseases. ...
journal_title:Autophagy
pub_type: 杂志文章
doi:10.4161/auto.7.7.15596
更新日期:2011-07-01 00:00:00
abstract::The understanding of the membrane flow process during autophagosome formation is essential to illuminate the role of autophagy under various disease-causing conditions. Atg9 is the only identified integral membrane protein required for autophagosome formation, and it is thought to cycle between the membrane sources an...
journal_title:Autophagy
pub_type: 杂志文章
doi:10.4161/auto.5.3.7699
更新日期:2009-04-01 00:00:00
abstract::Cigarette smoke (CS)-induced mitochondrial damage with increased reactive oxygen species (ROS) production has been implicated in COPD pathogenesis by accelerating senescence. Mitophagy may play a pivotal role for removal of CS-induced damaged mitochondria, and the PINK1 (PTEN-induced putative kinase 1)-PARK2 pathway h...
journal_title:Autophagy
pub_type: 杂志文章
doi:10.1080/15548627.2015.1017190
更新日期:2015-01-01 00:00:00
abstract::Autophagy is a highly conserved intracellular degradation system, and recently was shown to display circadian rhythms in mice. The mechanisms underlying circadian regulation of autophagy, however, are still unclear. Here, we observed that numbers of autophagosomes and autolysosomes exhibit daily rhythms in the zebrafi...
journal_title:Autophagy
pub_type: 杂志文章
doi:10.1080/15548627.2016.1183843
更新日期:2016-08-02 00:00:00
abstract::Autophagy is a fundamental process for anti-viral defense. Not surprisingly, viruses have developed strategies to subvert or use autophagy for their own benefit. In cell culture, autophagy proteins are proviral factors that favor initiation of hepatitis C virus (HCV) infection. Autophagy proteins are required for tran...
journal_title:Autophagy
pub_type: 杂志文章
doi:10.4161/auto.5.8.10219
更新日期:2009-11-01 00:00:00
abstract::Increasing evidence suggests that misfolded proteins and intracellular aggregates contribute to cardiac disease and heart failure. We wished to determine if autophagic induction by Atg7 is sufficient to reduce misfolded protein and aggregate content in protein misfolding-stressed cardiomyocytes. We used loss- and gain...
journal_title:Autophagy
pub_type: 杂志文章
doi:10.4161/auto.7.10.16882
更新日期:2011-10-01 00:00:00
abstract::Numerous cardiomyocytes were found to show autophagic vacuolar degeneration in the UM-X7.1 hamster model of human dilated cardiomyopathy, and autophagy-related proteins--i.e., ubiquitin, cathepsin D and Rab7--were upregulated in those hearts. Importantly, Evans blue-positive cardiomyocytes with leaky plasma membranes ...
journal_title:Autophagy
pub_type: 杂志文章,评审
doi:10.4161/auto.2608
更新日期:2006-07-01 00:00:00
abstract::Macroautophagy/autophagy mediates the degradation of ubiquitinated aggregated proteins within lysosomes in a process known as aggrephagy. The cargo receptor SQSTM1/p62 condenses aggregated proteins into larger structures and links them to the nascent autophagosomal membrane (phagophore). How the condensation reaction ...
journal_title:Autophagy
pub_type: 杂志文章
doi:10.1080/15548627.2019.1615306
更新日期:2019-08-01 00:00:00
abstract::Macroautophagy/autophagy is critical in maintaining cellular functions and homeostasis. Dynamic regulation of autophagy is associated with development of nonalcoholic fatty liver disease (NAFLD) and nonalcoholic steatohepatitis (NASH); however, the mechanisms involved in the regulation of autophagy in NAFLD/NASH are n...
journal_title:Autophagy
pub_type: 杂志文章
doi:10.1080/15548627.2020.1760624
更新日期:2020-07-01 00:00:00
abstract::Cutaneous inflammation from UV radiation exposure causes epidermal damage, cellular infiltration, and secretion of pro-inflammatory mediators that exacerbate tissue destruction. Recovery is mediated chiefly by anti-inflammatory M2 macrophages that suppress inflammation and augment epidermal regeneration. Vitamin D ena...
journal_title:Autophagy
pub_type: 杂志文章
doi:10.1080/15548627.2019.1569298
更新日期:2019-05-01 00:00:00
abstract::Autophagy is a catabolic process employed by eukaryotes to degrade and recycle intracellular components. When this pathway is induced by starvation conditions, part of the cytoplasm and organelles are sequestered into double-membrane vesicles called autophagosomes, and delivered into the lysosome/vacuole for degradati...
journal_title:Autophagy
pub_type: 杂志文章
doi:10.4161/auto.6657
更新日期:2008-10-01 00:00:00
abstract::Prion protein modulates many cellular functions including the secretion of trophic factors by astrocytes. Some of these factors are found in exosomes, which are formed within multivesicular bodies (MVBs) and secreted into the extracellular space to modulate cell-cell communication. The mechanisms underlying exosome bi...
journal_title:Autophagy
pub_type: 杂志文章
doi:10.1080/15548627.2016.1226735
更新日期:2016-11-01 00:00:00
abstract::The elimination of tumor cells by apoptosis is the main mechanism of action of chemotherapeutic drugs used in current treatment protocols of acute lymphoblastic leukemia (ALL). Despite the rapid cytoreduction achieved, serious acute and late complications are frequent, and resistance to chemotherapy develops. During t...
journal_title:Autophagy
pub_type: 杂志文章
doi:10.4161/auto.5.5.8507
更新日期:2009-07-01 00:00:00