Interspecies hybridization and recombination in Saccharomyces wine yeasts.

abstract：:We developed a method for the quantification of protein turnover rates using (13)C-labeled substrates combined with the analysis of the labeling patterns of proteinogenic amino acids. Using this method, the specific amino acid turnover rates between proteins and the pool of free amino acids were determined for eight d...

journal_title：FEMS yeast research

authors： Hong KK,Hou J,Shoaie S,Nielsen J,Bordel S

更新日期：2012-11-01 00:00:00

abstract：:We have cloned the Hansenula polymorpha SWI1 and SNF2 genes by functional complementation of mutants that are defective in methanol utilisation. These genes encode proteins similar to Saccharomyces cerevisiae Swi1p and Snf2p, which are subunits of the SWI/SNF complex. This complex belongs to the family of nucleosome-r...

journal_title：FEMS yeast research

authors： Ozimek P,Lahtchev K,Kiel JA,Veenhuis M,van der Klei IJ

更新日期：2004-05-01 00:00:00

abstract：:The budding yeast, Saccharomyces cerevisiae, is an efficient model for studying oxidative stress, programmed cell death and aging. The present study was carried out to investigate antioxidant, the anti-apoptotic and anti-aging activity of a natural compound, astaxanthin, in S. cerevisiae model. The survivability of ye...

journal_title：FEMS yeast research

authors： Sj S,Veerabhadrappa B,Subramaniyan S,Dyavaiah M

更新日期：2019-01-01 00:00:00

abstract：:Cadmium ions disrupt reactive oxygen species/Ca(2+) homeostasis and subsequently elicit cell death and adaptive signaling cascades in eukaryotic cells. Through a functional genomics approach, we have identified deletion mutants of 106 yeast genes, including three MAP kinase genes (HOG1, SLT2, and KSS1), are sensitive ...

journal_title：FEMS yeast research

authors： Jiang L,Cao C,Zhang L,Lin W,Xia J,Xu H,Zhang Y

更新日期：2014-12-01 00:00:00

abstract：:Candida albicans is an important opportunistic yeast pathogen of humans and has the ability to form drug-resistant biofilms, with increased expression of multidrug ATP-binding cassette (ABC) transporters. These biofilms are also capable of secreting immune-modulating prostaglandin E2 (PGE2 ) from host-derived arachido...

journal_title：FEMS yeast research

authors： Ells R,Kemp G,Albertyn J,Kock JL,Pohl CH

更新日期：2013-12-01 00:00:00

abstract：:Aldose-1-epimerase or mutarotase (EC 5.1.3.3) catalyzes interconversion of alpha/beta-anomers of aldoses, such as glucose and galactose, and is distributed in a wide variety of organisms from bacteria to humans. Nevertheless, the physiological role of this enzyme has been elusive in most cases, because the alpha-form ...

journal_title：FEMS yeast research

authors： Fukasawa T,Sakurai H,Nogi Y,Baruffini E

更新日期：2009-08-01 00:00:00

abstract：:Genes of the post-squalene ergosterol biosynthetic pathway in Saccharomyces cerevisiae have been overexpressed in a systematic approach with the aim to construct yeast strains that produce high amounts of sterols from a squalene-accumulating strain. This strain had previously been deregulated by overexpressing a trunc...

journal_title：FEMS yeast research

authors： Veen M,Stahl U,Lang C

更新日期：2003-10-01 00:00:00

abstract：:The replicative lifespan of Saccharomyces cerevisiae is determined by both genetic and environmental factors. Many of the same factors determine the lifespan of metazoan animals. The lack of fast and reliable lifespan assays has limited the pace of yeast aging research. In this study we describe a novel strategy for a...

journal_title：FEMS yeast research

authors： Jarolim S,Millen J,Heeren G,Laun P,Goldfarb DS,Breitenbach M

更新日期：2004-11-01 00:00:00

abstract：:Since the discovery of the apoptotic pathway in Saccharomyces cerevisiae, several compounds have been shown to cause apoptosis in this organism. While the toxicity of polyunsaturated fatty acids (PUFA) peroxides towards S. cerevisiae has been known for a long time, studies on the effect of nonoxidized PUFA are scarce....

journal_title：FEMS yeast research

authors： Ferreira TC,de Moraes LM,Campos EG

更新日期：2011-08-01 00:00:00

abstract：:Yeasts of the genus Candida are a major cause of morbidity and mortality in immunocompromised patients. Despite new insights in recent years, the pathogenesis of Candida infection is still incompletely understood. Previous studies have suggested that gliotoxin, a secondary fungal metabolite with well-known immunosuppr...

journal_title：FEMS yeast research

authors： Kupfahl C,Ruppert T,Dietz A,Geginat G,Hof H

更新日期：2007-09-01 00:00:00

abstract：:Wild-type strains of the thermotolerant methylotrophic yeast Hansenula polymorpha are able to ferment glucose, cellobiose and xylose to ethanol. H. polymorpha most actively fermented sugars to ethanol at 37 degrees C, whereas the well-known xylose-fermenting yeast Pichia stipitis could not effectively ferment carbon s...

journal_title：FEMS yeast research

authors： Ryabova OB,Chmil OM,Sibirny AA

更新日期：2003-11-01 00:00:00

abstract：:CLC-type chloride/proton antiporters are required for copper/iron homeostasis in fungi. A relationship between CLCs and Ca(2+) homeostasis has not been found before. Here we demonstrate the requirement of the antiporter CLC1 for Ca(2+) homeostasis/signaling in Cryptococcus neoformans. The deletion of CLC1 in JEC21 r...

journal_title：FEMS yeast research

authors： Li D,Zhang X,Li Z,Yang J,Pan J,Zhu X

更新日期：2012-02-01 00:00:00

abstract：:Among existing fungal pathogens, Candida glabrata is outstanding in its capacity to rapidly develop resistance to currently used antifungal agents. Resistance to the class of azoles, which are still widely used agents, varies in proportion (from 5 to 20%) depending on geographical area. Moreover, resistance to the cla...

journal_title：FEMS yeast research

authors： Vale-Silva LA,Sanglard D

更新日期：2015-06-01 00:00:00

abstract：:The Tapirapé people of the Tapi'itãwa tribe of Brazil produce several fermented foods and beverages, one of which is called 'cauim'. This beverage usually makes up the main staple food for adults and children. Several substrates are used in its production, including cassava, rice, corn, maize and peanuts. A fermentati...

journal_title：FEMS yeast research

authors： Schwan RF,Almeida EG,Souza-Dias MA,Jespersen L

更新日期：2007-09-01 00:00:00

abstract：:Candida glabrata is a second most common human opportunistic pathogen which causes superficial but also life-threatening systemic candidosis. According to the localisation of mannans and mannoproteins in the outermost layer of the cell wall, mannan detection could be one of the first steps in the cell recognition of C...

journal_title：FEMS yeast research

authors： Paulovičová L,Paulovičová E,Farkaš P,Čížová A,Bystrický P,Jančinová V,Turánek J,Pericolini E,Gabrielli E,Vecchiarelli A,Hrubiško M

更新日期：2019-03-01 00:00:00

abstract：:The essentiality of iodine for humans, especially in the early stages of life, is well recognized. The chemical forms of iodine in food supplements, infant formulae and iodated salt are either iodide (KI) or iodate (KIO(3)). Because there are no or rare data about iodine uptake by yeasts, we investigated the influence...

journal_title：FEMS yeast research

authors： Osterc A,Fujs S,Raspor P,Stibilj V

更新日期：2009-02-01 00:00:00

abstract：:Every year more than 2 million tons of citric acid (CA) are produced around the world for industrial uses. Although initially extracted from citrus, the low profitability of the process and the increasing demand soon stimulated the search for more efficient methods to produce CA. Currently, most world CA demand (99%) ...

journal_title：FEMS yeast research

authors： Cavallo E,Charreau H,Cerrutti P,Foresti ML

更新日期：2017-12-01 00:00:00

abstract：:This study addresses the question of whether observable changes in fluxes in the primary carbon metabolism of Saccharomyces cerevisiae occur between the different phases of the cell division cycle. To detect such changes by metabolic flux analysis, a 13C-labeling experiment was performed with a fed-batch culture inocu...

journal_title：FEMS yeast research

authors： Costenoble R,Müller D,Barl T,van Gulik WM,van Winden WA,Reuss M,Heijnen JJ

更新日期：2007-06-01 00:00:00

abstract：:The catabolism of methionine to methionol and methanethiol in Saccharomyces cerevisiae was studied using (13)C NMR spectroscopy, GC-MS, enzyme assays and a number of mutants. Methionine is first transaminated to alpha-keto-gamma-(methylthio)butyrate. Methionol is formed by a decarboxylation reaction, which yields meth...

journal_title：FEMS yeast research

authors： Perpète P,Duthoit O,De Maeyer S,Imray L,Lawton AI,Stavropoulos KE,Gitonga VW,Hewlins MJ,Dickinson JR

更新日期：2006-01-01 00:00:00

abstract：:Mitochondria are dynamic organelles of endosymbiotic origin that are essential components of eukaryal cells. They contain their own genetic machinery, have multicopy genomes and like their bacterial ancestors they consist of two membranes. However, the majority of the ancestral genome has been lost or transferred to t...

journal_title：FEMS yeast research

authors： Malina C,Larsson C,Nielsen J

更新日期：2018-08-01 00:00:00

abstract：:Saccharomyces cerevisiae grown on plastic surfaces formed organized structures, termed minicolonies, that consisted of a core of round (yeast-like) cells surrounded by chains of filamentous cells (pseudohyphae). Minicolonies had a much higher affinity for plastic than unstructured yeast communities growing on the same...

journal_title：FEMS yeast research

authors： White MG,Piccirillo S,Dusevich V,Law DJ,Kapros T,Honigberg SM

更新日期：2011-03-01 00:00:00

abstract：:Current paradigms assume that gas bubbles cannot be formed within yeasts although these workhorses of the baking and brewing industries vigorously produce and release CO(2) gas. We show that yeasts produce gas bubbles that fill a significant part of the cell. The missing link between intracellular CO(2) production b...

journal_title：FEMS yeast research

authors： Swart CW,Dithebe K,Pohl CH,Swart HC,Coetsee E,van Wyk PW,Swarts JC,Lodolo EJ,Kock JL

更新日期：2012-11-01 00:00:00

abstract：:Three ascomycetous yeast strains were isolated from decaying green wine grapes, collected from Hyderabad city in India. Two strains, YS9 and YS21, were identified as Kodamaea ohmeri and Candida fermentati, respectively. The third strain, YS12(T), differs from Candida parapsilosis, Candida orthopsilosis and Candida met...

journal_title：FEMS yeast research

authors： Rao RS,Bhadra B,Kumar NN,Shivaji S

更新日期：2007-05-01 00:00:00

abstract：:The asci of Metschnikowia species normally contain two ascospores (never more), raising the question of whether these spores are true meiotic products. We investigated this problem by crossing genetically-marked strains of the haploid, heterothallic taxa Metschnikowia hawaiiensis, Metschnikowia continentalis var. cont...

journal_title：FEMS yeast research

authors： Marinoni G,Piskur J,Lachance MA

更新日期：2003-03-01 00:00:00

abstract：:The yeast Zygosaccharomyces bailii, known to have peculiar resistance to several environmental constraints, is very little known with respect to its genetics and life cycle. In addition to molecular and biochemical studies, cytofluorimetric and morphological analyses can also add information necessary to shed light on...

journal_title：FEMS yeast research

authors： Dato L,Sauer M,Passolunghi S,Porro D,Branduardi P

更新日期：2008-09-01 00:00:00

abstract：:Dimethyl sulphoxide is extensively used in chemical, pharmaceutical and biomedical applications, but its specific biological actions remain largely elusive. The aim of this study was to comprehensively explore the effects of dimethyl sulphoxide on eukaryotic growth and senescence by using the budding yeast Saccharomyc...

journal_title：FEMS yeast research

authors： Kakolyri M,Margaritou A,Tiligada E

更新日期：2016-03-01 00:00:00

abstract：:Nine yeast strains were isolated from spontaneous fermentations in the Alsace area of France, during the 1997, 1998 and 1999 grape harvests. Strains were characterized by pulsed-field gel electrophoresis, PCR-restriction fragment length polymorphism (RFLP) of the MET2 gene, delta-PCR, and microsatellite patterns. Kary...

journal_title：FEMS yeast research

authors： Le Jeune C,Lollier M,Demuyter C,Erny C,Legras JL,Aigle M,Masneuf-Pomarède I

更新日期：2007-06-01 00:00:00

abstract：:The expression of the transcription regulator Pdr1p and its target genes PDR5 and TPO1 is required for Saccharomyces cerevisiae adaptation and resistance to artesunate, a promising antimalarial drug, also active against tumour cells and viruses. PDR5 and TPO1 encode plasma membrane multidrug transporters of the ATP-bi...

journal_title：FEMS yeast research

authors： Alenquer M,Tenreiro S,Sá-Correia I

更新日期：2006-12-01 00:00:00

abstract：:A complexity of pathway expression in yeast compared to prokaryotes is the need for separate promoters and terminators for each gene expressed. Single transcript expression and separated protein production is possible via the use of 2A viral peptides, but detailed characterization to assess their suitability and appli...

journal_title：FEMS yeast research

authors： Souza-Moreira TM,Navarrete C,Chen X,Zanelli CF,Valentini SR,Furlan M,Nielsen J,Krivoruchko A

更新日期：2018-08-01 00:00:00

abstract：:High initial cell density is used to increase volumetric productivity and shorten production time in lignocellulosic hydrolysate fermentation. Comparison of physiological parameters in high initial cell density cultivation of Saccharomyces cerevisiae in the presence of acetic, formic, levulinic and cinnamic acids demo...

journal_title：FEMS yeast research

authors： Guo ZP,Olsson L

更新日期：2016-11-01 00:00:00