A novel assay for replicative lifespan in Saccharomyces cerevisiae.

abstract：:During their mitotic cycle, cylindrical fission yeast cells grow exclusively at their tips. Length growth starts at birth and halts at mitotic onset when the cells begin to prepare for division. While the growth pattern was initially considered to be exponential, during the last three decades an increasing amount of e...

journal_title：FEMS yeast research

authors： Horváth A,Rácz-Mónus A,Buchwald P,Sveiczer Á

更新日期：2013-11-01 00:00:00

abstract：:Cdc37p, the p50 homolog of Saccharomyces cerevisiae, is an Hsp90 cochaperone involved in the targeting of protein kinases to Hsp90. Here we report a role for Cdc37p in osmoadaptive signalling in this yeast. The osmosensitive phenotype that is displayed by the cdc37-34 mutant strain appears not to be the consequence of...

journal_title：FEMS yeast research

authors： Yang XX,Hawle P,Bebelman JP,Meenhuis A,Siderius M,van der Vies SM

更新日期：2007-09-01 00:00:00

abstract：:Maintenance and adaptation of energy metabolism could play an important role in the cellular ability to respond to DNA damage. A large number of studies suggest that the sensitivity of cells to oxidants and oxidative stress depends on the activity of cellular metabolism and is dependent on the glucose concentration. I...

journal_title：FEMS yeast research

authors： Kitanovic A,Walther T,Loret MO,Holzwarth J,Kitanovic I,Bonowski F,Van Bui N,Francois JM,Wölfl S

更新日期：2009-06-01 00:00:00

abstract：:In previous communications the essential role of spermidine in Ustilago maydis was demonstrated by means of the disruption of the genes encoding ornithine decarboxylase (ODC) and spermidine synthase (SPE). However, the assignation of specific roles to each polyamine in different cellular functions was not possible bec...

journal_title：FEMS yeast research

authors： Valdés-Santiago L,Guzmán-de-Peña D,Ruiz-Herrera J

更新日期：2010-11-01 00:00:00

abstract：:Three ascomycetous yeast strains were isolated from decaying green wine grapes, collected from Hyderabad city in India. Two strains, YS9 and YS21, were identified as Kodamaea ohmeri and Candida fermentati, respectively. The third strain, YS12(T), differs from Candida parapsilosis, Candida orthopsilosis and Candida met...

journal_title：FEMS yeast research

authors： Rao RS,Bhadra B,Kumar NN,Shivaji S

更新日期：2007-05-01 00:00:00

abstract：:The asci of Metschnikowia species normally contain two ascospores (never more), raising the question of whether these spores are true meiotic products. We investigated this problem by crossing genetically-marked strains of the haploid, heterothallic taxa Metschnikowia hawaiiensis, Metschnikowia continentalis var. cont...

journal_title：FEMS yeast research

authors： Marinoni G,Piskur J,Lachance MA

更新日期：2003-03-01 00:00:00

abstract：:High initial cell density is used to increase volumetric productivity and shorten production time in lignocellulosic hydrolysate fermentation. Comparison of physiological parameters in high initial cell density cultivation of Saccharomyces cerevisiae in the presence of acetic, formic, levulinic and cinnamic acids demo...

journal_title：FEMS yeast research

authors： Guo ZP,Olsson L

更新日期：2016-11-01 00:00:00

abstract：:Microorganisms have been studied and used extensively to produce value-added fuels and chemicals. Yeasts, specifically Saccharomyces cerevisiae, receive industrial attention because of their well-known ability to ferment glucose and produce ethanol. Thousands of natural or genetically modified S. cerevisiae have been ...

journal_title：FEMS yeast research

authors： Kong II,Turner TL,Kim H,Kim SR,Jin YS

更新日期：2018-02-01 00:00:00

abstract：:The genus Schizosaccharomyces is presently comprised of three species, namely Schizosaccharomyces pombe, Schizosaccharomyces octosporus and Schizosaccharomyces japonicus. Here, we describe a hitherto unknown species, Schizosaccharomyces cryophilus, named for its preference for growth at lower temperatures than the oth...

journal_title：FEMS yeast research

authors： Helston RM,Box JA,Tang W,Baumann P

更新日期：2010-09-01 00:00:00

abstract：:Interspecies hybridization is an important evolutionary mechanism in yeasts. The genus Zygosaccharomyces in particular contains numerous hybrid strains and/or species. Here, we investigated the genome of Zygosaccharomyces strain MT15, an isolate from Maotai-flavor Chinese liquor fermentation. We found that it is an in...

journal_title：FEMS yeast research

authors： Braun-Galleani S,Ortiz-Merino RA,Wu Q,Xu Y,Wolfe KH

更新日期：2018-11-01 00:00:00

abstract：:The ultimate goal of therapeutic vaccines is to activate and exploit the patient's own immune system to vigorously and dynamically seek and eradicate established malignant or virally infected cells. Therapeutic vaccines also offer the potential for preventing disease recurrence. Saccharomyces cerevisiae-based vaccines...

journal_title：FEMS yeast research

authors： Ardiani A,Higgins JP,Hodge JW

更新日期：2010-12-01 00:00:00

abstract：:The essentiality of iodine for humans, especially in the early stages of life, is well recognized. The chemical forms of iodine in food supplements, infant formulae and iodated salt are either iodide (KI) or iodate (KIO(3)). Because there are no or rare data about iodine uptake by yeasts, we investigated the influence...

journal_title：FEMS yeast research

authors： Osterc A,Fujs S,Raspor P,Stibilj V

更新日期：2009-02-01 00:00:00

abstract：:Zinc is a crucial mineral for all organisms as it is an essential cofactor for the proper function of a plethora of proteins and depletion of zinc causes oxidative stress. Glutathione is the major redox buffering agent in the cell and therefore important for mitigation of the adverse effects of oxidative stress. In ma...

journal_title：FEMS yeast research

authors： Steiger MG,Patzschke A,Holz C,Lang C,Causon T,Hann S,Mattanovich D,Sauer M

更新日期：2017-06-01 00:00:00

abstract：:This study displays a screening using yeast strains deficient in protein kinases known to exist in Saccharomyces cerevisiae. From 95 viable single mutants, 20 mutants appear to be affected in the glucose-induced extracellular acidification. The mutants that are unaffected in calcium signaling were tested for their sen...

journal_title：FEMS yeast research

authors： Pereira RR,Castanheira D,Teixeira JA,Bouillet LE,Ribeiro EM,Trópia MM,Alvarez F,Correa LF,Mota BE,Conceição LE,Castro IM,Brandão RL

更新日期：2015-03-01 00:00:00

abstract：:Among existing fungal pathogens, Candida glabrata is outstanding in its capacity to rapidly develop resistance to currently used antifungal agents. Resistance to the class of azoles, which are still widely used agents, varies in proportion (from 5 to 20%) depending on geographical area. Moreover, resistance to the cla...

journal_title：FEMS yeast research

authors： Vale-Silva LA,Sanglard D

更新日期：2015-06-01 00:00:00

abstract：:Here we present evidence that Hsp150/Pir2, a member of the Pir family of cell wall proteins, can be extracted from the purified cell walls of Saccharomyces cerevisiae by treatment with beta-mercaptoethanol, demonstrating that at least part of this protein is attached to the cell wall through disulfide bridges. We also...

journal_title：FEMS yeast research

authors： Moukadiri I,Zueco J

更新日期：2001-12-01 00:00:00

abstract：:The most diffused formulation of starter for winemaking is active dry yeast (ADY). ADYs production process is essentially characterized by air-drying stress, a combination of several stresses, including thermal, hyperosmotic and oxidative and cell capacity to counteract such multiple stresses will determine its surviv...

journal_title：FEMS yeast research

authors： Capece A,Votta S,Guaragnella N,Zambuto M,Romaniello R,Romano P

更新日期：2016-05-01 00:00:00

abstract：:First-generation (1G) fuel ethanol production in sugarcane-based biorefineries is an established economic enterprise in Brazil. Second-generation (2G) fuel ethanol from lignocellulosic materials, though extensively investigated, is currently facing severe difficulties to become economically viable. Some of the challen...

journal_title：FEMS yeast research

authors： Bermejo PM,Raghavendran V,Gombert AK

更新日期：2020-06-01 00:00:00

abstract：:Viruses are found in almost all organisms and physical habitats. One interesting example is the yeast viral 'killer system'. The virus provides the host with a toxin directed against strains that do not carry it, while the yeast cell enables its propagation. Although yeast viruses are believed to be common, they have ...

journal_title：FEMS yeast research

authors： Pieczynska MD,de Visser JA,Korona R

更新日期：2013-12-01 00:00:00

abstract：:A genetic and cell-biological analysis is provided for Saccharomyces cerevisiae DML1 (YMR211w) encoding a Drosophila melanogaster Misato-like protein. Misato and Dml1p are descendants of an ancestral tubulin-like protein, and exhibit regions with similarity to members of a GTPase family that include eukaryotic tubulin...

journal_title：FEMS yeast research

authors： Gurvitz A,Hartig A,Ruis H,Hamilton B,de Couet HG

更新日期：2002-05-01 00:00:00

abstract：:Extracellular proteins of Cryptococcus neoformans are involved in the pathogenesis of cryptococcosis, and some are immunoreactive antigens that may potentially serve as candidates for vaccine development. To further study the extracellular proteome of the human fungal pathogen Cry. neoformans, we conducted a proteomic...

journal_title：FEMS yeast research

authors： Eigenheer RA,Jin Lee Y,Blumwald E,Phinney BS,Gelli A

更新日期：2007-06-01 00:00:00

abstract：:Conversion of glucose to lactic acid is stoichiometrically equivalent to ethanol formation with respect to ATP formation from substrate-level phosphorylation, redox equivalents and product yield. However, anaerobic growth cannot be sustained in homolactate fermenting Saccharomyces cerevisiae. ATP-dependent export of t...

journal_title：FEMS yeast research

authors： Abbott DA,van den Brink J,Minneboo IM,Pronk JT,van Maris AJ

更新日期：2009-05-01 00:00:00

abstract：:We show that Arf3p, a member of the ADP ribosylation family, is involved in the organization of actin cables and cortical patches in Saccharomyces cerevisiae. Profilin-deficient cells (pfy1Delta) have severe growth defects and lack actin cables. Overexpression of ARF3 restores actin cables and corrects growth defects ...

journal_title：FEMS yeast research

authors： Lambert AA,Perron MP,Lavoie E,Pallotta D

更新日期：2007-09-01 00:00:00

abstract：:The feasibility of using a single vector to clone a cytochrome P450 monooxygenase (P450) in different yeasts and then compare whole-cell hydroxylase activity was investigated. A broad-range yeast expression vector using the ylTEFp to drive expression of the cloned gene and the scTEFp to drive the hygromycin resistance...

journal_title：FEMS yeast research

authors： Theron CW,Labuschagné M,Gudiminchi R,Albertyn J,Smit MS

更新日期：2014-06-01 00:00:00

abstract：:The budding yeast, Saccharomyces cerevisiae, is an efficient model for studying oxidative stress, programmed cell death and aging. The present study was carried out to investigate antioxidant, the anti-apoptotic and anti-aging activity of a natural compound, astaxanthin, in S. cerevisiae model. The survivability of ye...

journal_title：FEMS yeast research

authors： Sj S,Veerabhadrappa B,Subramaniyan S,Dyavaiah M

更新日期：2019-01-01 00:00:00

abstract：:Proteostasis is a cellular housekeeping process that refers to the healthy maintenance of the cellular proteome that governs the fate of proteins from synthesis to degradation. Perturbations of proteostasis might result in protein dysfunction with consequent deleterious effects that can culminate in cell death. To dea...

journal_title：FEMS yeast research

authors： Sampaio-Marques B,Ludovico P

更新日期：2018-08-01 00:00:00

abstract：:In the macroscopic world, ecological interactions between multiple species of fauna and flora are recognised as major role-players in the evolution of any particular species. By comparison, research on ecological interactions as a driver of evolutionary adaptation in microbial ecosystems has been neglected. The evolut...

journal_title：FEMS yeast research

authors： Conacher CG,Rossouw D,Bauer FFB

更新日期：2019-11-01 00:00:00

abstract：:The LYS12 gene from Candida albicans, coding for homoisocitrate dehydrogenase was cloned and expressed as a His-tagged protein in Escherichia coli. The purified gene product catalyzes the Mg(2+)- and K(+)-dependent oxidative decarboxylation of homoisocitrate to α-ketoadipate. The recombinant enzyme demonstrates strict...

journal_title：FEMS yeast research

authors： Gabriel I,Vetter ND,Palmer DR,Milewska MJ,Wojciechowski M,Milewski S

更新日期：2013-03-01 00:00:00

abstract：:A biofilm-forming strain of Pichia fermentans proved to be most effective in controlling brown rot on apple fruit when coinoculated into artificial wounds with a phytopathogenic isolate of Monilinia fructicola. Culture filtrates and autoclaved cells had no significant influence on the disease. When sprayed onto the ap...

journal_title：FEMS yeast research

authors： Giobbe S,Marceddu S,Scherm B,Zara G,Mazzarello VL,Budroni M,Migheli Q

更新日期：2007-12-01 00:00:00

abstract：:Candida albicans is an important opportunistic yeast pathogen of humans and has the ability to form drug-resistant biofilms, with increased expression of multidrug ATP-binding cassette (ABC) transporters. These biofilms are also capable of secreting immune-modulating prostaglandin E2 (PGE2 ) from host-derived arachido...

journal_title：FEMS yeast research

authors： Ells R,Kemp G,Albertyn J,Kock JL,Pohl CH

更新日期：2013-12-01 00:00:00