Abstract:
:In previous communications the essential role of spermidine in Ustilago maydis was demonstrated by means of the disruption of the genes encoding ornithine decarboxylase (ODC) and spermidine synthase (SPE). However, the assignation of specific roles to each polyamine in different cellular functions was not possible because the spermidine added to satisfy the auxotrophic requirement of odc/spe double mutants is partly back converted into putrescine. In this study, we have approached this problem through the disruption of the gene-encoding polyamine oxidase (PAO), required for the conversion of spermidine into putrescine, and the construction of odc/pao double mutants that were unable to synthesize putrescine by either ornithine decarboxylation or retroconversion from spermidine. Phenotypic analysis of the mutants provided evidence that putrescine is only an intermediary in spermidine biosynthesis, and has no direct role in cell growth, dimorphic transition, or any other vital function of U. maydis. Nevertheless, our results show that putrescine may play a role in the protection of U. maydis against salt and osmotic stress, and possibly virulence. Evidence was also obtained that the retroconversion of spermidine into putrescine is not essential for U. maydis growth but may be important for its survival under natural conditions.
journal_name
FEMS Yeast Resjournal_title
FEMS yeast researchauthors
Valdés-Santiago L,Guzmán-de-Peña D,Ruiz-Herrera Jdoi
10.1111/j.1567-1364.2010.00675.xsubject
Has Abstractpub_date
2010-11-01 00:00:00pages
928-40issue
7eissn
1567-1356issn
1567-1364journal_volume
10pub_type
杂志文章abstract::Lignocellulosic plant biomass is the target feedstock for production of second-generation biofuels. Ionic liquid (IL) pretreatment can enhance deconstruction of lignocellulosic biomass into sugars that can be fermented to ethanol. Although biomass is typically washed following IL pretreatment, small quantities of resi...
journal_title:FEMS yeast research
pub_type: 杂志文章
doi:10.1111/1567-1364.12224
更新日期:2014-12-01 00:00:00
abstract::Five yeast isolates obtained from cashew tree flowers in Nigeria resembled Cryptococcus neoformans phenotypically by producing brown pigmented colonies on niger seed agar, expressing a capsule, and being able to grow at 37 degrees C. However, rRNA gene sequences, including the 18S rRNA gene, the D1/D2 domains of the 2...
journal_title:FEMS yeast research
pub_type: 杂志文章
doi:10.1111/j.1567-1364.2006.00164.x
更新日期:2007-03-01 00:00:00
abstract::The Saccharomyces cerevisiae allantoate/ureidosuccinate permease gene (DAL5) is often used as a reporter in studies of the Tor1/2 protein kinases which are specifically inhibited by the clinically important immunosuppressant and anti-neoplastic drug, rapamycin. To date, only a single type of cis-acting element has bee...
journal_title:FEMS yeast research
pub_type: 杂志文章
doi:10.1016/j.femsyr.2004.06.004
更新日期:2004-10-01 00:00:00
abstract::The replicative lifespan of Saccharomyces cerevisiae is determined by both genetic and environmental factors. Many of the same factors determine the lifespan of metazoan animals. The lack of fast and reliable lifespan assays has limited the pace of yeast aging research. In this study we describe a novel strategy for a...
journal_title:FEMS yeast research
pub_type: 杂志文章
doi:10.1016/j.femsyr.2004.06.015
更新日期:2004-11-01 00:00:00
abstract::The aim of this retrospective study was to analyze the relationship between occurrence of the serotypes of the Cryptococcus neoformans species complex in tree samples and the climatic conditions registered during samplings in four cities of Colombia, between 1992 and 2004, by means of a logistic regression model and l...
journal_title:FEMS yeast research
pub_type: 杂志文章
doi:10.1111/j.1567-1364.2006.00090.x
更新日期:2006-06-01 00:00:00
abstract::Endosomal sorting complexes required for transport (ESCRTs) are involved in the formation of multivesicular bodies and sorting of targeted proteins to the yeast vacuole. The deletion of seven genes encoding components of the ESCRT machinery render Saccharomyces cerevisiae cells sensitive to high extracellular CaCl2 co...
journal_title:FEMS yeast research
pub_type: 杂志文章
doi:10.1093/femsyr/fov007
更新日期:2015-03-01 00:00:00
abstract::We studied the effect of the loss of the Ser-Thr protein phosphatase Sit4, an important post-translational regulator, on the steady-state levels of the low-affinity glucose transporter Hxt1p and observed a delay in its appearance after high glucose induction, slow growth, and diminished glucose consumption. By analyzi...
journal_title:FEMS yeast research
pub_type: 杂志文章
doi:10.1111/j.1567-1364.2012.00839.x
更新日期:2012-12-01 00:00:00
abstract::A complexity of pathway expression in yeast compared to prokaryotes is the need for separate promoters and terminators for each gene expressed. Single transcript expression and separated protein production is possible via the use of 2A viral peptides, but detailed characterization to assess their suitability and appli...
journal_title:FEMS yeast research
pub_type: 杂志文章
doi:10.1093/femsyr/foy036
更新日期:2018-08-01 00:00:00
abstract::Fungal wall formation is a dynamic process involving several categories of enzymes. The GH72 family of β(1,3)-glucanosyltransferases is essential for the determination of cell shape, for cell integrity and for virulence in pathogenic fungi. Candida albicans has five GH72 genes: PHR1 and PHR2 are pH dependent, the firs...
journal_title:FEMS yeast research
pub_type: 杂志文章
doi:10.1093/femsyr/fou011
更新日期:2015-03-01 00:00:00
abstract::Saccharomyces cerevisiae is an excellent ethanol producer, but is rather sensitive to high concentration of ethanol. Here, influences of ethanol on cellular membrane integrity and carbon metabolism of S. cerevisiae were investigated to rationalize mechanism involved in ethanol toxicity. Addition of 5% (v/v) ethanol di...
journal_title:FEMS yeast research
pub_type: 杂志文章
doi:10.1111/j.1567-1364.2012.00818.x
更新日期:2012-09-01 00:00:00
abstract::Each time a cell duplicates, the whole genome must be accurately copied and distributed. The enormous amount of DNA in eukaryotic cells requires a high level of coordination between polymerases and other DNA and chromatin-interacting proteins to ensure timely and accurate DNA replication and chromatin formation. PCNA ...
journal_title:FEMS yeast research
pub_type: 杂志文章,评审
doi:10.1093/femsyr/fow084
更新日期:2016-11-01 00:00:00
abstract::The yeast glucose transporters Hxt1, Hxt2, Hxt3, Hxt4, Hxt6, Hxt7 and Gal2, individually expressed in an hxt1-7 null mutant strain, demonstrate the phenomenon of countertransport. Thus, these transporters, which are the most important glucose transporters in Saccharomyces cerevisiae, are facilitated diffusion transpor...
journal_title:FEMS yeast research
pub_type: 杂志文章
doi:10.1111/j.1567-1364.2002.tb00121.x
更新日期:2002-12-01 00:00:00
abstract::Every year more than 2 million tons of citric acid (CA) are produced around the world for industrial uses. Although initially extracted from citrus, the low profitability of the process and the increasing demand soon stimulated the search for more efficient methods to produce CA. Currently, most world CA demand (99%) ...
journal_title:FEMS yeast research
pub_type: 杂志文章
doi:10.1093/femsyr/fox084
更新日期:2017-12-01 00:00:00
abstract::The fungus Paracoccidioides brasiliensis causes paracoccidioidomycosis, a systemic granulomatous mycosis prevalent in Latin America. In an effort to elucidate the molecular mechanisms involved in fungus cell wall assembly and morphogenesis, beta-1,3-glucanosyltransferase 3 (PbGel3p) is presented here. PbGel3p presente...
journal_title:FEMS yeast research
pub_type: 杂志文章
doi:10.1111/j.1567-1364.2008.00463.x
更新日期:2009-02-01 00:00:00
abstract::A biofilm-forming strain of Pichia fermentans proved to be most effective in controlling brown rot on apple fruit when coinoculated into artificial wounds with a phytopathogenic isolate of Monilinia fructicola. Culture filtrates and autoclaved cells had no significant influence on the disease. When sprayed onto the ap...
journal_title:FEMS yeast research
pub_type: 杂志文章
doi:10.1111/j.1567-1364.2007.00301.x
更新日期:2007-12-01 00:00:00
abstract::Secondary metabolites are an important source of high-value chemicals, many of which exhibit important pharmacological properties. These valuable natural products are often difficult to synthesize chemically and are commonly isolated through inefficient extractions from natural biological sources. As such, they are in...
journal_title:FEMS yeast research
pub_type: 杂志文章,评审
doi:10.1111/j.1567-1364.2011.00774.x
更新日期:2012-03-01 00:00:00
abstract::We developed a method for the quantification of protein turnover rates using (13)C-labeled substrates combined with the analysis of the labeling patterns of proteinogenic amino acids. Using this method, the specific amino acid turnover rates between proteins and the pool of free amino acids were determined for eight d...
journal_title:FEMS yeast research
pub_type: 杂志文章
doi:10.1111/j.1567-1364.2012.00823.x
更新日期:2012-11-01 00:00:00
abstract::Penicillin G amidase from Providencia rettgeri is a heterodimer of 92 kDa. We have previously expressed the Pr. rettgeri pac gene coding for this enzyme in Saccharomyces cerevisiae, and now we report the expression and characterization in the methylotrophic yeast Pichia pastoris. The recombinant catalytically active e...
journal_title:FEMS yeast research
pub_type: 杂志文章
doi:10.1111/j.1567-1364.2002.tb00045.x
更新日期:2002-01-01 00:00:00
abstract::Heat shock response (HSR) is an important element of cellular homeostasis. In yeast, HSR comprises of the heat shock proteins (Hsps) and the osmolytes trehalose and glycerol. The respective roles of trehalose and Hsp104 in regulating protein aggregation remain ambiguous. We report that trehalose and Hsp104 are importa...
journal_title:FEMS yeast research
pub_type: 杂志文章
doi:10.1093/femsyr/foy058
更新日期:2018-09-01 00:00:00
abstract::Loss of mtDNA by the petite-negative yeast Kluyveromyces lactis is lethal (rho(o)-lethality). However, mutations in the alpha, beta and gamma subunits of F(1)-ATPase can suppress lethality by increasing intramitochondrial hydrolysis of ATP. Increased hydrolysis of ATP can also occur on inactivation of Inh1, the natura...
journal_title:FEMS yeast research
pub_type: 杂志文章
doi:10.1111/j.1567-1364.2006.00201.x
更新日期:2007-08-01 00:00:00
abstract::The mechanistic details of mtDNA maintenance in petite-negative yeasts have remained largely unexplored. We report here that the DNA helicase Hmi1p plays a crucial role in mtDNA stability in Candida albicans. Like its counterpart in Saccharomyces cerevisiae, Hmi1p in C. albicans (CaHmi1p) contains a C-terminal mitocho...
journal_title:FEMS yeast research
pub_type: 杂志文章
doi:10.1111/j.1567-1364.2006.00132.x
更新日期:2007-01-01 00:00:00
abstract::Viruses are found in almost all organisms and physical habitats. One interesting example is the yeast viral 'killer system'. The virus provides the host with a toxin directed against strains that do not carry it, while the yeast cell enables its propagation. Although yeast viruses are believed to be common, they have ...
journal_title:FEMS yeast research
pub_type: 杂志文章
doi:10.1111/1567-1364.12086
更新日期:2013-12-01 00:00:00
abstract::Aldose-1-epimerase or mutarotase (EC 5.1.3.3) catalyzes interconversion of alpha/beta-anomers of aldoses, such as glucose and galactose, and is distributed in a wide variety of organisms from bacteria to humans. Nevertheless, the physiological role of this enzyme has been elusive in most cases, because the alpha-form ...
journal_title:FEMS yeast research
pub_type: 杂志文章
doi:10.1111/j.1567-1364.2009.00517.x
更新日期:2009-08-01 00:00:00
abstract::Extracellular proteins of Cryptococcus neoformans are involved in the pathogenesis of cryptococcosis, and some are immunoreactive antigens that may potentially serve as candidates for vaccine development. To further study the extracellular proteome of the human fungal pathogen Cry. neoformans, we conducted a proteomic...
journal_title:FEMS yeast research
pub_type: 杂志文章
doi:10.1111/j.1567-1364.2006.00198.x
更新日期:2007-06-01 00:00:00
abstract::Regulation of virulence factor production in the pathogen Cryptococcus neoformans remains to be fully illustrated. We present here a finding that a gene, encoding the glycolysis enzyme phosphoglucose isomerase (Pgi1), is critical for the biosynthesis of melanin and capsule, cell wall integrity and resistance to stress...
journal_title:FEMS yeast research
pub_type: 社论
doi:10.1093/femsyr/fov072
更新日期:2015-11-01 00:00:00
abstract::Yeasts belonging to the lineage that underwent whole-genome duplication (WGD) possess a good fermentative potential and can proliferate in the absence of oxygen. In this study, we analyzed the pre-WGD yeast Kluyveromyces lactis and its ability to grow under oxygen-limited conditions. Under these conditions, K. lactis ...
journal_title:FEMS yeast research
pub_type: 杂志文章
doi:10.1111/j.1567-1364.2009.00528.x
更新日期:2009-08-01 00:00:00
abstract::Dysfunction or capacity shortage of the endoplasmic reticulum (ER) is cumulatively called ER stress and provokes the unfolded protein response (UPR). In various yeast species, the ER-located transmembrane protein Ire1 is activated upon ER stress and performs the splicing reaction of HAC1 mRNA, the mature form of which...
journal_title:FEMS yeast research
pub_type: 杂志文章
doi:10.1093/femsyr/foaa053
更新日期:2020-10-15 00:00:00
abstract::Cadmium ions disrupt reactive oxygen species/Ca(2+) homeostasis and subsequently elicit cell death and adaptive signaling cascades in eukaryotic cells. Through a functional genomics approach, we have identified deletion mutants of 106 yeast genes, including three MAP kinase genes (HOG1, SLT2, and KSS1), are sensitive ...
journal_title:FEMS yeast research
pub_type: 杂志文章
doi:10.1111/1567-1364.12220
更新日期:2014-12-01 00:00:00
abstract::This study displays a screening using yeast strains deficient in protein kinases known to exist in Saccharomyces cerevisiae. From 95 viable single mutants, 20 mutants appear to be affected in the glucose-induced extracellular acidification. The mutants that are unaffected in calcium signaling were tested for their sen...
journal_title:FEMS yeast research
pub_type: 杂志文章
doi:10.1093/femsyr/fov003
更新日期:2015-03-01 00:00:00
abstract::Trk1 and Trk2 are the major K(+) transport systems in Schizosaccharomyces pombe. Both transporters individually seem to be able to cope with K(+) requirements of the cells under normal conditions, since only the double mutant shows defective K(+) transport and defective growth at limiting K(+) concentrations. We have ...
journal_title:FEMS yeast research
pub_type: 杂志文章
doi:10.1016/j.femsyr.2003.11.003
更新日期:2004-03-01 00:00:00