A broad-range yeast expression system reveals Arxula adeninivorans expressing a fungal self-sufficient cytochrome P450 monooxygenase as an excellent whole-cell biocatalyst.

abstract：:The LYS12 gene from Candida albicans, coding for homoisocitrate dehydrogenase was cloned and expressed as a His-tagged protein in Escherichia coli. The purified gene product catalyzes the Mg(2+)- and K(+)-dependent oxidative decarboxylation of homoisocitrate to α-ketoadipate. The recombinant enzyme demonstrates strict...

journal_title：FEMS yeast research

authors： Gabriel I,Vetter ND,Palmer DR,Milewska MJ,Wojciechowski M,Milewski S

更新日期：2013-03-01 00:00:00

abstract：:The expression of the transcription regulator Pdr1p and its target genes PDR5 and TPO1 is required for Saccharomyces cerevisiae adaptation and resistance to artesunate, a promising antimalarial drug, also active against tumour cells and viruses. PDR5 and TPO1 encode plasma membrane multidrug transporters of the ATP-bi...

journal_title：FEMS yeast research

authors： Alenquer M,Tenreiro S,Sá-Correia I

更新日期：2006-12-01 00:00:00

abstract：:Candida albicans maintains genetic diversity by random chromosome alterations, and this diversity allows utilization of various nutrients. Although the alterations seem to occur spontaneously, their frequencies clearly depend on environmental factors. In addition, this microorganism survives in adverse environments, w...

journal_title：FEMS yeast research

authors： Rustchenko E

更新日期：2007-01-01 00:00:00

abstract：:The Saccharomyces cerevisiae allantoate/ureidosuccinate permease gene (DAL5) is often used as a reporter in studies of the Tor1/2 protein kinases which are specifically inhibited by the clinically important immunosuppressant and anti-neoplastic drug, rapamycin. To date, only a single type of cis-acting element has bee...

journal_title：FEMS yeast research

authors： Rai R,Daugherty JR,Tate JJ,Buford TD,Cooper TG

更新日期：2004-10-01 00:00:00

abstract：:The most diffused formulation of starter for winemaking is active dry yeast (ADY). ADYs production process is essentially characterized by air-drying stress, a combination of several stresses, including thermal, hyperosmotic and oxidative and cell capacity to counteract such multiple stresses will determine its surviv...

journal_title：FEMS yeast research

authors： Capece A,Votta S,Guaragnella N,Zambuto M,Romaniello R,Romano P

更新日期：2016-05-01 00:00:00

abstract：:The yeast glucose transporters Hxt1, Hxt2, Hxt3, Hxt4, Hxt6, Hxt7 and Gal2, individually expressed in an hxt1-7 null mutant strain, demonstrate the phenomenon of countertransport. Thus, these transporters, which are the most important glucose transporters in Saccharomyces cerevisiae, are facilitated diffusion transpor...

journal_title：FEMS yeast research

authors： Maier A,Völker B,Boles E,Fuhrmann GF

更新日期：2002-12-01 00:00:00

abstract：:Molecular tools for the production of heterologous proteins and metabolic engineering applications of the non-conventional yeast Zygosaccharomyces bailii were developed. The combination of Z. bailii's resistance to relatively high temperature, osmotic pressure and low pH values, with a high specific growth rate render...

journal_title：FEMS yeast research

authors： Branduardi P,Valli M,Brambilla L,Sauer M,Alberghina L,Porro D

更新日期：2004-01-01 00:00:00

abstract：:The influence of three commonly used fungicides (iprodione, pyrimethanil and fludioxonil plus cyprodinil) on the density and diversity of yeast populations present on grape berries was evaluated. At the time of harvest, the fungicide residues on grapes were below the maximum permitted levels. In general, larger yeast ...

journal_title：FEMS yeast research

authors： Cadez N,Zupan J,Raspor P

更新日期：2010-08-01 00:00:00

abstract：:A complexity of pathway expression in yeast compared to prokaryotes is the need for separate promoters and terminators for each gene expressed. Single transcript expression and separated protein production is possible via the use of 2A viral peptides, but detailed characterization to assess their suitability and appli...

journal_title：FEMS yeast research

authors： Souza-Moreira TM,Navarrete C,Chen X,Zanelli CF,Valentini SR,Furlan M,Nielsen J,Krivoruchko A

更新日期：2018-08-01 00:00:00

abstract：:In Kluyveromyces lactis, galactose transport has been thought to be mediated by the lactose permease encoded by LAC12. In fact, a lac12 mutant unable to grow on lactose did not grow on galactose either and showed low and uninducible galactose uptake activity. The existence of other galactose transport systems, at low ...

journal_title：FEMS yeast research

authors： Baruffini E,Goffrini P,Donnini C,Lodi T

更新日期：2006-12-01 00:00:00

abstract：:In Saccharomyces cerevisiae, the cell integrity pathway plays a role in the oxidative stress response. In this study, we show that the Pkc1 protein mediates oxidative signalling by helping to downregulate ribosomal gene expression when cells are exposed to hydrogen peroxide. An active actin cytoskeleton is required fo...

journal_title：FEMS yeast research

authors： Mitjana FV,Petkova MI,Pujol-Carrion N,de la Torre-Ruiz MA

更新日期：2011-12-01 00:00:00

abstract：:Regulation of virulence factor production in the pathogen Cryptococcus neoformans remains to be fully illustrated. We present here a finding that a gene, encoding the glycolysis enzyme phosphoglucose isomerase (Pgi1), is critical for the biosynthesis of melanin and capsule, cell wall integrity and resistance to stress...

journal_title：FEMS yeast research

authors： Zhang P,Wei D,Li Z,Sun Z,Pan J,Zhu X

更新日期：2015-11-01 00:00:00

abstract：:An extended definition of the term metabolic engineering is given and its successful use in the construction of biorecognition elements of sensors is demonstrated. It is shown that genetic and chemical modifications of methylotrophic yeast cells provide directed changes in their physiological responses towards methano...

journal_title：FEMS yeast research

authors： Gonchar M,Maidan M,Korpan Y,Sibirny V,Kotylak Z,Sibirny A

更新日期：2002-08-01 00:00:00

abstract：:The target of rapamycin complex 1 (TORC1) is the central controller of growth in eukaryotic cells. As one of the downstream targets of TORC1, the protein kinase ScSch9p plays multiple roles in stress resistance, longevity and nutrient sensing in Saccharomyces cerevisiae. In this study, we demonstrate that Candida albi...

journal_title：FEMS yeast research

authors： Liu W,Zhao J,Li X,Li Y,Jiang L

更新日期：2010-06-01 00:00:00

abstract：:The genus Schizosaccharomyces is presently comprised of three species, namely Schizosaccharomyces pombe, Schizosaccharomyces octosporus and Schizosaccharomyces japonicus. Here, we describe a hitherto unknown species, Schizosaccharomyces cryophilus, named for its preference for growth at lower temperatures than the oth...

journal_title：FEMS yeast research

authors： Helston RM,Box JA,Tang W,Baumann P

更新日期：2010-09-01 00:00:00

abstract：:Trk1 and Trk2 are the major K(+) transport systems in Schizosaccharomyces pombe. Both transporters individually seem to be able to cope with K(+) requirements of the cells under normal conditions, since only the double mutant shows defective K(+) transport and defective growth at limiting K(+) concentrations. We have ...

journal_title：FEMS yeast research

authors： Calero F,Montiel V,Caracuel Z,Cabello-Hurtado F,Ramos J

更新日期：2004-03-01 00:00:00

abstract：:A biofilm-forming strain of Pichia fermentans proved to be most effective in controlling brown rot on apple fruit when coinoculated into artificial wounds with a phytopathogenic isolate of Monilinia fructicola. Culture filtrates and autoclaved cells had no significant influence on the disease. When sprayed onto the ap...

journal_title：FEMS yeast research

authors： Giobbe S,Marceddu S,Scherm B,Zara G,Mazzarello VL,Budroni M,Migheli Q

更新日期：2007-12-01 00:00:00

abstract：:Dysfunction or capacity shortage of the endoplasmic reticulum (ER) is cumulatively called ER stress and provokes the unfolded protein response (UPR). In various yeast species, the ER-located transmembrane protein Ire1 is activated upon ER stress and performs the splicing reaction of HAC1 mRNA, the mature form of which...

journal_title：FEMS yeast research

authors： Fauzee YNBM,Taniguchi N,Ishiwata-Kimata Y,Takagi H,Kimata Y

更新日期：2020-10-15 00:00:00

abstract：:The fungal cell wall is a coherent structure formed by microfibrillar polysaccharides and amorphous material made of other polysaccharides and proteins. We performed a phylogenetic analysis of covalent proteins and enzymes that synthesize fungal wall polysaccharides to determine the possible evolution of the wall stru...

journal_title：FEMS yeast research

authors： Ruiz-Herrera J,Ortiz-Castellanos L

更新日期：2010-05-01 00:00:00

abstract：:Current paradigms assume that gas bubbles cannot be formed within yeasts although these workhorses of the baking and brewing industries vigorously produce and release CO(2) gas. We show that yeasts produce gas bubbles that fill a significant part of the cell. The missing link between intracellular CO(2) production b...

journal_title：FEMS yeast research

authors： Swart CW,Dithebe K,Pohl CH,Swart HC,Coetsee E,van Wyk PW,Swarts JC,Lodolo EJ,Kock JL

更新日期：2012-11-01 00:00:00

abstract：:The durum wheat TMKP1 gene encodes a MAP kinase phosphatase. When overexpressed in Saccharomyces cerevisiae, TMKP1 leads to salt stress tolerance (especially LiCl ), which is dependent on the phosphatase activity of the protein. The TMKP1-associated Li(+) resistance is restricted to a galactose-containing medium. Inte...

journal_title：FEMS yeast research

authors： Zaidi I,González A,Touzri M,Alvarez MC,Ramos J,Masmoudi K,Ariño J,Hanin M

更新日期：2012-11-01 00:00:00

abstract：:Candida glabrata is a second most common human opportunistic pathogen which causes superficial but also life-threatening systemic candidosis. According to the localisation of mannans and mannoproteins in the outermost layer of the cell wall, mannan detection could be one of the first steps in the cell recognition of C...

journal_title：FEMS yeast research

authors： Paulovičová L,Paulovičová E,Farkaš P,Čížová A,Bystrický P,Jančinová V,Turánek J,Pericolini E,Gabrielli E,Vecchiarelli A,Hrubiško M

更新日期：2019-03-01 00:00:00

abstract：:The closely related yeasts Debaryomyces fabryi and Debaryomyces hansenii are excellent xylose consumers. We previously described the activity of a high-affinity xylose/H(+) symport from an industrial strain of D. hansenii subsequently reclassified as D. fabryi. We now report the identification of the gene encoding thi...

journal_title：FEMS yeast research

authors： Ferreira D,Nobre A,Silva ML,Faria-Oliveira F,Tulha J,Ferreira C,Lucas C

更新日期：2013-11-01 00:00:00

abstract：:Mutants of Saccharomyces cerevisiae with inactivated endopolyphosphatase gene PPN1 did not grow on lactate and ethanol, and stopped growth on glucose earlier than the parent strain. Their mitochondria were defective in respiration functions and in metabolism of inorganic polyphosphates. The PPN1 mutants lacked exopoly...

journal_title：FEMS yeast research

authors： Pestov NA,Kulakovskaya TV,Kulaev IS

更新日期：2005-06-01 00:00:00

abstract：:Here we present evidence that Hsp150/Pir2, a member of the Pir family of cell wall proteins, can be extracted from the purified cell walls of Saccharomyces cerevisiae by treatment with beta-mercaptoethanol, demonstrating that at least part of this protein is attached to the cell wall through disulfide bridges. We also...

journal_title：FEMS yeast research

authors： Moukadiri I,Zueco J

更新日期：2001-12-01 00:00:00

abstract：:The yeast Zygosaccharomyces bailii, known to have peculiar resistance to several environmental constraints, is very little known with respect to its genetics and life cycle. In addition to molecular and biochemical studies, cytofluorimetric and morphological analyses can also add information necessary to shed light on...

journal_title：FEMS yeast research

authors： Dato L,Sauer M,Passolunghi S,Porro D,Branduardi P

更新日期：2008-09-01 00:00:00

abstract：:Lignocellulosic plant biomass is the target feedstock for production of second-generation biofuels. Ionic liquid (IL) pretreatment can enhance deconstruction of lignocellulosic biomass into sugars that can be fermented to ethanol. Although biomass is typically washed following IL pretreatment, small quantities of resi...

journal_title：FEMS yeast research

authors： Sitepu IR,Shi S,Simmons BA,Singer SW,Boundy-Mills K,Simmons CW

更新日期：2014-12-01 00:00:00

abstract：:The biosynthesis of yeast phosphatidylglycerol (PG) takes place in the inner mitochondrial membrane. Outside mitochondria, the abundance of PG is low. Here, we present evidence that the subcellular distribution of PG is maintained by the locally controlled enzymatic activity of the PG-specific phospholipase, Pgc1. A f...

journal_title：FEMS yeast research

authors： Kubalová D,Káňovičová P,Veselá P,Awadová T,Džugasová V,Daum G,Malínský J,Balážová M

更新日期：2019-08-01 00:00:00

abstract：:The yeast Pichia pastoris is a widely used host organism for heterologous protein production. One of the basic steps for strain improvement is to ensure a sufficient level of transcription of the heterologous gene, based on promoter strength and gene copy number. To date, high-copy-number integrants of P. pastoris are...

journal_title：FEMS yeast research

authors： Marx H,Mecklenbräuker A,Gasser B,Sauer M,Mattanovich D

更新日期：2009-12-01 00:00:00

abstract：:The immunobiological efficacy of synthetically prepared mannooligosaccharides and a glucooligosaccharide mimicking the structure of Candida albicans cell wall glycans was assessed in vivo and in vitro to exploit immune responses. The exposure of mice splenocytes to BSA-based conjugates of synthetic oligomannosides and...

journal_title：FEMS yeast research

authors： Paulovičová E,Paulovičová L,Pilišiová R,Bystrický S,Yashunsky DV,Karelin AA,Tsvetkov YE,Nifantiev NE

更新日期：2013-11-01 00:00:00