Cryptococcal phosphoglucose isomerase is required for virulence factor production, cell wall integrity and stress resistance.

abstract：:Lignocellulosic plant biomass is the target feedstock for production of second-generation biofuels. Ionic liquid (IL) pretreatment can enhance deconstruction of lignocellulosic biomass into sugars that can be fermented to ethanol. Although biomass is typically washed following IL pretreatment, small quantities of resi...

journal_title：FEMS yeast research

authors： Sitepu IR,Shi S,Simmons BA,Singer SW,Boundy-Mills K,Simmons CW

更新日期：2014-12-01 00:00:00

abstract：:The ascomycetous yeasts traditionally referred to as the Saccharomyces sensu stricto complex are a group of closely related species that are isolated by a postzygotic barrier. They can easily hybridize; and their allodiploid hybrids propagate by mitotic divisions as efficiently as the parental strains, but can barely ...

journal_title：FEMS yeast research

authors： Sipiczki M

更新日期：2008-11-01 00:00:00

abstract：:An astaxanthin-overproducing (∼1000 μg g(-1)) strain of Phaffia rhodozyma, termed MK19, was established through 1-methyl-3-nitro-1-nitrosoguanidine and Co60 mutagenesis from wild-type JCM9042 (merely 35-67 μg g(-1)). The total fatty acid content of MK19 was much lower than that of the wild type. Possible causes of the...

journal_title：FEMS yeast research

authors： Miao L,Chi S,Tang Y,Su Z,Yin T,Guan G,Li Y

更新日期：2011-03-01 00:00:00

abstract：:The genus Schizosaccharomyces is presently comprised of three species, namely Schizosaccharomyces pombe, Schizosaccharomyces octosporus and Schizosaccharomyces japonicus. Here, we describe a hitherto unknown species, Schizosaccharomyces cryophilus, named for its preference for growth at lower temperatures than the oth...

journal_title：FEMS yeast research

authors： Helston RM,Box JA,Tang W,Baumann P

更新日期：2010-09-01 00:00:00

abstract：:The mechanism involved in the cellular phosphate response of Saccharomyces cerevisiae forms part of the PHO pathway, which upon expression allows a co-ordinated cellular response and adaptation to changes in availability of external phosphate. Although genetic studies and analyses of the S. cerevisiae genome have prod...

journal_title：FEMS yeast research

authors： Mouillon JM,Persson BL

更新日期：2006-03-01 00:00:00

abstract：:The most diffused formulation of starter for winemaking is active dry yeast (ADY). ADYs production process is essentially characterized by air-drying stress, a combination of several stresses, including thermal, hyperosmotic and oxidative and cell capacity to counteract such multiple stresses will determine its surviv...

journal_title：FEMS yeast research

authors： Capece A,Votta S,Guaragnella N,Zambuto M,Romaniello R,Romano P

更新日期：2016-05-01 00:00:00

abstract：:The GPD1 gene encoding the glycerol-3-phosphate dehydrogenase was overexpressed in an industrial lager brewing yeast (Saccharomyces cerevisiae ssp. carlsbergensis) to reduce the content of ethanol in beer. The amount of glycerol produced by the GPD1-overexpressing yeast in fermentation experiments simulating brewing c...

journal_title：FEMS yeast research

authors： Nevoigt E,Pilger R,Mast-Gerlach E,Schmidt U,Freihammer S,Eschenbrenner M,Garbe L,Stahl U

更新日期：2002-05-01 00:00:00

abstract：:Loss of mtDNA by the petite-negative yeast Kluyveromyces lactis is lethal (rho(o)-lethality). However, mutations in the alpha, beta and gamma subunits of F(1)-ATPase can suppress lethality by increasing intramitochondrial hydrolysis of ATP. Increased hydrolysis of ATP can also occur on inactivation of Inh1, the natura...

journal_title：FEMS yeast research

authors： Clark-Walker GD

更新日期：2007-08-01 00:00:00

abstract：:The target of rapamycin complex 1 (TORC1) is the central controller of growth in eukaryotic cells. As one of the downstream targets of TORC1, the protein kinase ScSch9p plays multiple roles in stress resistance, longevity and nutrient sensing in Saccharomyces cerevisiae. In this study, we demonstrate that Candida albi...

journal_title：FEMS yeast research

authors： Liu W,Zhao J,Li X,Li Y,Jiang L

更新日期：2010-06-01 00:00:00

abstract：:Conversion of glucose to lactic acid is stoichiometrically equivalent to ethanol formation with respect to ATP formation from substrate-level phosphorylation, redox equivalents and product yield. However, anaerobic growth cannot be sustained in homolactate fermenting Saccharomyces cerevisiae. ATP-dependent export of t...

journal_title：FEMS yeast research

authors： Abbott DA,van den Brink J,Minneboo IM,Pronk JT,van Maris AJ

更新日期：2009-05-01 00:00:00

abstract：:In Kluyveromyces lactis, galactose transport has been thought to be mediated by the lactose permease encoded by LAC12. In fact, a lac12 mutant unable to grow on lactose did not grow on galactose either and showed low and uninducible galactose uptake activity. The existence of other galactose transport systems, at low ...

journal_title：FEMS yeast research

authors： Baruffini E,Goffrini P,Donnini C,Lodi T

更新日期：2006-12-01 00:00:00

abstract：:Non-Saccharomyces yeasts impact wine fermentations and can diversify the flavor profiles of wines. However, little information is available on the metabolic networks of most of these species. Here we show that unlike the main wine yeast Saccharomyces cerevisiae, Torulaspora delbrueckii and to a lesser extent Lachancea...

journal_title：FEMS yeast research

authors： Mbuyane LL,de Kock M,Bauer FF,Divol B

更新日期：2018-11-01 00:00:00

abstract：:Promoter of alcohol oxidase I (PAOX1) is the most efficient promoter involved in the regulation of recombinant protein expression in Pichia pastoris (P. pastoris). PAOX1 is tightly repressed by the presence of glycerol in the culture medium; thus, glycerol must be exhausted before methanol can be taken up by P. pastor...

journal_title：FEMS yeast research

authors： Zhan C,Wang S,Sun Y,Dai X,Liu X,Harvey L,McNeil B,Yang Y,Bai Z

更新日期：2016-06-01 00:00:00

abstract：:Malassezia pachydermatis isolates (n=185) from skin sites from dogs (n=30) were characterized genetically and biochemically following in vitro culture. Two regions in the chitin synthase-2 gene (chs-2) and the first internal transcribed spacer (ITS-1) of nuclear ribosomal DNA were sequenced, and the phospholipase acti...

journal_title：FEMS yeast research

authors： Cafarchia C,Gasser RB,Latrofa MS,Parisi A,Campbell BE,Otranto D

更新日期：2008-05-01 00:00:00

abstract：:Mutants of Saccharomyces cerevisiae with inactivated endopolyphosphatase gene PPN1 did not grow on lactate and ethanol, and stopped growth on glucose earlier than the parent strain. Their mitochondria were defective in respiration functions and in metabolism of inorganic polyphosphates. The PPN1 mutants lacked exopoly...

journal_title：FEMS yeast research

authors： Pestov NA,Kulakovskaya TV,Kulaev IS

更新日期：2005-06-01 00:00:00

abstract：:In the macroscopic world, ecological interactions between multiple species of fauna and flora are recognised as major role-players in the evolution of any particular species. By comparison, research on ecological interactions as a driver of evolutionary adaptation in microbial ecosystems has been neglected. The evolut...

journal_title：FEMS yeast research

authors： Conacher CG,Rossouw D,Bauer FFB

更新日期：2019-11-01 00:00:00

abstract：:Candida glabrata is a second most common human opportunistic pathogen which causes superficial but also life-threatening systemic candidosis. According to the localisation of mannans and mannoproteins in the outermost layer of the cell wall, mannan detection could be one of the first steps in the cell recognition of C...

journal_title：FEMS yeast research

authors： Paulovičová L,Paulovičová E,Farkaš P,Čížová A,Bystrický P,Jančinová V,Turánek J,Pericolini E,Gabrielli E,Vecchiarelli A,Hrubiško M

更新日期：2019-03-01 00:00:00

abstract：:In the pathogenic fungus Candida glabrata, the YPS1 gene, which encodes a glycosylphosphatidylinositol-linked aspartyl protease, is required for cell wall integrity and virulence. Although the expression of YPS1 has been studied in Saccharomyces cerevisiae, the transcriptional regulation of this gene in C. glabrata is...

journal_title：FEMS yeast research

authors： Miyazaki T,Izumikawa K,Yamauchi S,Inamine T,Nagayoshi Y,Saijo T,Seki M,Kakeya H,Yamamoto Y,Yanagihara K,Miyazaki Y,Yasuoka A,Kohno S

更新日期：2011-08-01 00:00:00

abstract：:We used multi-locus sequence typing (MLST) to investigate 35 yeast isolates representing the two genome-sequenced strains plus the type strain of Candida albicans, four isolates originally identified as Candida stellatoidea type I and 28 representing type strains of other species now regarded as synonymous with C. alb...

journal_title：FEMS yeast research

authors： Jacobsen MD,Boekhout T,Odds FC

更新日期：2008-08-01 00:00:00

abstract：:The replicative lifespan of Saccharomyces cerevisiae is determined by both genetic and environmental factors. Many of the same factors determine the lifespan of metazoan animals. The lack of fast and reliable lifespan assays has limited the pace of yeast aging research. In this study we describe a novel strategy for a...

journal_title：FEMS yeast research

authors： Jarolim S,Millen J,Heeren G,Laun P,Goldfarb DS,Breitenbach M

更新日期：2004-11-01 00:00:00

abstract：:Saccharomyces cerevisiae grown on plastic surfaces formed organized structures, termed minicolonies, that consisted of a core of round (yeast-like) cells surrounded by chains of filamentous cells (pseudohyphae). Minicolonies had a much higher affinity for plastic than unstructured yeast communities growing on the same...

journal_title：FEMS yeast research

authors： White MG,Piccirillo S,Dusevich V,Law DJ,Kapros T,Honigberg SM

更新日期：2011-03-01 00:00:00

abstract：:During their mitotic cycle, cylindrical fission yeast cells grow exclusively at their tips. Length growth starts at birth and halts at mitotic onset when the cells begin to prepare for division. While the growth pattern was initially considered to be exponential, during the last three decades an increasing amount of e...

journal_title：FEMS yeast research

authors： Horváth A,Rácz-Mónus A,Buchwald P,Sveiczer Á

更新日期：2013-11-01 00:00:00

abstract：:The Tapirapé people of the Tapi'itãwa tribe of Brazil produce several fermented foods and beverages, one of which is called 'cauim'. This beverage usually makes up the main staple food for adults and children. Several substrates are used in its production, including cassava, rice, corn, maize and peanuts. A fermentati...

journal_title：FEMS yeast research

authors： Schwan RF,Almeida EG,Souza-Dias MA,Jespersen L

更新日期：2007-09-01 00:00:00

abstract：:Extracellular proteins of Cryptococcus neoformans are involved in the pathogenesis of cryptococcosis, and some are immunoreactive antigens that may potentially serve as candidates for vaccine development. To further study the extracellular proteome of the human fungal pathogen Cry. neoformans, we conducted a proteomic...

journal_title：FEMS yeast research

authors： Eigenheer RA,Jin Lee Y,Blumwald E,Phinney BS,Gelli A

更新日期：2007-06-01 00:00:00

abstract：:The pathogenic yeast Cryptococcus neoformans has evolved several strategies to survive within phagocytes. Recently, it has been demonstrated that upregulation of the ATP binding cassette transporter-encoding gene antifungal resistance 1 (AFR1) is important not only for determining the resistance of C. neoformans to fl...

journal_title：FEMS yeast research

authors： Orsi CF,Colombari B,Ardizzoni A,Peppoloni S,Neglia R,Posteraro B,Morace G,Fadda G,Blasi E

更新日期：2009-03-01 00:00:00

abstract：:The asci of Metschnikowia species normally contain two ascospores (never more), raising the question of whether these spores are true meiotic products. We investigated this problem by crossing genetically-marked strains of the haploid, heterothallic taxa Metschnikowia hawaiiensis, Metschnikowia continentalis var. cont...

journal_title：FEMS yeast research

authors： Marinoni G,Piskur J,Lachance MA

更新日期：2003-03-01 00:00:00

abstract：:Trk1 and Trk2 are the major K(+) transport systems in Schizosaccharomyces pombe. Both transporters individually seem to be able to cope with K(+) requirements of the cells under normal conditions, since only the double mutant shows defective K(+) transport and defective growth at limiting K(+) concentrations. We have ...

journal_title：FEMS yeast research

authors： Calero F,Montiel V,Caracuel Z,Cabello-Hurtado F,Ramos J

更新日期：2004-03-01 00:00:00

abstract：:The catabolism of methionine to methionol and methanethiol in Saccharomyces cerevisiae was studied using (13)C NMR spectroscopy, GC-MS, enzyme assays and a number of mutants. Methionine is first transaminated to alpha-keto-gamma-(methylthio)butyrate. Methionol is formed by a decarboxylation reaction, which yields meth...

journal_title：FEMS yeast research

authors： Perpète P,Duthoit O,De Maeyer S,Imray L,Lawton AI,Stavropoulos KE,Gitonga VW,Hewlins MJ,Dickinson JR

更新日期：2006-01-01 00:00:00

abstract：:Murine experimentation has provided many useful tools, including the ability to knockout or over-express genes and to perform experiments that are limited by ethical considerations. Over the past century, mice have imparted valuable insights into the biology of many systems, including human immunity. However, although...

journal_title：FEMS yeast research

authors： Marr KJ,Jones GJ,Mody CH

更新日期：2006-06-01 00:00:00

abstract：:Yeasts of the genus Candida are a major cause of morbidity and mortality in immunocompromised patients. Despite new insights in recent years, the pathogenesis of Candida infection is still incompletely understood. Previous studies have suggested that gliotoxin, a secondary fungal metabolite with well-known immunosuppr...

journal_title：FEMS yeast research

authors： Kupfahl C,Ruppert T,Dietz A,Geginat G,Hof H

更新日期：2007-09-01 00:00:00