Anaerobic homolactate fermentation with Saccharomyces cerevisiae results in depletion of ATP and impaired metabolic activity.

abstract：:Viruses are found in almost all organisms and physical habitats. One interesting example is the yeast viral 'killer system'. The virus provides the host with a toxin directed against strains that do not carry it, while the yeast cell enables its propagation. Although yeast viruses are believed to be common, they have ...

journal_title：FEMS yeast research

authors： Pieczynska MD,de Visser JA,Korona R

更新日期：2013-12-01 00:00:00

abstract：:We studied the effect of the loss of the Ser-Thr protein phosphatase Sit4, an important post-translational regulator, on the steady-state levels of the low-affinity glucose transporter Hxt1p and observed a delay in its appearance after high glucose induction, slow growth, and diminished glucose consumption. By analyzi...

journal_title：FEMS yeast research

authors： Souza AA,Miranda MN,da Silva SF,Bozaquel-Morais B,Masuda CA,Ghislain M,Montero-Lomelí M

更新日期：2012-12-01 00:00:00

abstract：:Yeasts belonging to the lineage that underwent whole-genome duplication (WGD) possess a good fermentative potential and can proliferate in the absence of oxygen. In this study, we analyzed the pre-WGD yeast Kluyveromyces lactis and its ability to grow under oxygen-limited conditions. Under these conditions, K. lactis ...

journal_title：FEMS yeast research

authors： Merico A,Galafassi S,Piskur J,Compagno C

更新日期：2009-08-01 00:00:00

abstract：:Fungal wall formation is a dynamic process involving several categories of enzymes. The GH72 family of β(1,3)-glucanosyltransferases is essential for the determination of cell shape, for cell integrity and for virulence in pathogenic fungi. Candida albicans has five GH72 genes: PHR1 and PHR2 are pH dependent, the firs...

journal_title：FEMS yeast research

authors： Kováčová K,Degani G,Stratilová E,Farkaš V,Popolo L

更新日期：2015-03-01 00:00:00

abstract：:Brettanomyces bruxellensis is usually considered a spoilage microorganism, responsible for significant economic losses during the production of fermented beverages such as wine, beer and cider, though for some styles of beer its influence is essential. In recent years, the competitiveness of this yeast in bioethanol p...

journal_title：FEMS yeast research

authors： Varela C,Lleixà J,Curtin C,Borneman A

更新日期：2018-11-01 00:00:00

abstract：:The histone acetyltransferase Sas2 is part of the SAS-I complex and acetylates lysine 16 of histone H4 (H4 K16Ac) in the genome of Saccharomyces cerevisiae. Sas2-mediated H4 K16Ac is strongest over the coding region of genes with low expression. However, it is unclear how Sas2-mediated acetylation is incorporated into...

journal_title：FEMS yeast research

authors： Reiter C,Heise F,Chung HR,Ehrenhofer-Murray AE

更新日期：2015-11-01 00:00:00

abstract：:Kluyveromyces marxianus is a safe yeast used in the food and biotechnology sectors. One of the important traits that sets it apart from the familiar yeasts, Saccharomyces cerevisiae, is its capacity to grow using lactose as a carbon source. Like in its close relative, Kluyveromyces lactis, this requires lactose transp...

journal_title：FEMS yeast research

authors： Varela JA,Montini N,Scully D,Van der Ploeg R,Oreb M,Boles E,Hirota J,Akada R,Hoshida H,Morrissey JP

更新日期：2017-05-01 00:00:00

abstract：:The mechanistic details of mtDNA maintenance in petite-negative yeasts have remained largely unexplored. We report here that the DNA helicase Hmi1p plays a crucial role in mtDNA stability in Candida albicans. Like its counterpart in Saccharomyces cerevisiae, Hmi1p in C. albicans (CaHmi1p) contains a C-terminal mitocho...

journal_title：FEMS yeast research

authors： Jõers P,Gerhold JM,Sedman T,Kuusk S,Sedman J

更新日期：2007-01-01 00:00:00

abstract：:The amplification by PCR of the Intergenic Spacer region (IGS) of rDNA followed by Restriction Fragment Length Polymorphism (RFLP) analysis was evaluated as a potential method for the identification of Debaryomyces hansenii among other yeast species that frequently contaminate Intermediate-Moisture Foods (IMFs). For a...

journal_title：FEMS yeast research

authors： Romero P,Patiño B,Quirós M,González-Jaén MT,Valderrama MJ,de Silóniz MI,Peinado JM

更新日期：2005-02-01 00:00:00

abstract：:Malassezia pachydermatis isolates (n=185) from skin sites from dogs (n=30) were characterized genetically and biochemically following in vitro culture. Two regions in the chitin synthase-2 gene (chs-2) and the first internal transcribed spacer (ITS-1) of nuclear ribosomal DNA were sequenced, and the phospholipase acti...

journal_title：FEMS yeast research

authors： Cafarchia C,Gasser RB,Latrofa MS,Parisi A,Campbell BE,Otranto D

更新日期：2008-05-01 00:00:00

abstract：:Cadmium ions disrupt reactive oxygen species/Ca(2+) homeostasis and subsequently elicit cell death and adaptive signaling cascades in eukaryotic cells. Through a functional genomics approach, we have identified deletion mutants of 106 yeast genes, including three MAP kinase genes (HOG1, SLT2, and KSS1), are sensitive ...

journal_title：FEMS yeast research

authors： Jiang L,Cao C,Zhang L,Lin W,Xia J,Xu H,Zhang Y

更新日期：2014-12-01 00:00:00

abstract：:The pathogenic yeast Cryptococcus neoformans has evolved several strategies to survive within phagocytes. Recently, it has been demonstrated that upregulation of the ATP binding cassette transporter-encoding gene antifungal resistance 1 (AFR1) is important not only for determining the resistance of C. neoformans to fl...

journal_title：FEMS yeast research

authors： Orsi CF,Colombari B,Ardizzoni A,Peppoloni S,Neglia R,Posteraro B,Morace G,Fadda G,Blasi E

更新日期：2009-03-01 00:00:00

abstract：:An extended definition of the term metabolic engineering is given and its successful use in the construction of biorecognition elements of sensors is demonstrated. It is shown that genetic and chemical modifications of methylotrophic yeast cells provide directed changes in their physiological responses towards methano...

journal_title：FEMS yeast research

authors： Gonchar M,Maidan M,Korpan Y,Sibirny V,Kotylak Z,Sibirny A

更新日期：2002-08-01 00:00:00

abstract：:Maintenance and adaptation of energy metabolism could play an important role in the cellular ability to respond to DNA damage. A large number of studies suggest that the sensitivity of cells to oxidants and oxidative stress depends on the activity of cellular metabolism and is dependent on the glucose concentration. I...

journal_title：FEMS yeast research

authors： Kitanovic A,Walther T,Loret MO,Holzwarth J,Kitanovic I,Bonowski F,Van Bui N,Francois JM,Wölfl S

更新日期：2009-06-01 00:00:00

abstract：:The target of rapamycin complex 1 (TORC1) is the central controller of growth in eukaryotic cells. As one of the downstream targets of TORC1, the protein kinase ScSch9p plays multiple roles in stress resistance, longevity and nutrient sensing in Saccharomyces cerevisiae. In this study, we demonstrate that Candida albi...

journal_title：FEMS yeast research

authors： Liu W,Zhao J,Li X,Li Y,Jiang L

更新日期：2010-06-01 00:00:00

abstract：:This study addresses the question of whether observable changes in fluxes in the primary carbon metabolism of Saccharomyces cerevisiae occur between the different phases of the cell division cycle. To detect such changes by metabolic flux analysis, a 13C-labeling experiment was performed with a fed-batch culture inocu...

journal_title：FEMS yeast research

authors： Costenoble R,Müller D,Barl T,van Gulik WM,van Winden WA,Reuss M,Heijnen JJ

更新日期：2007-06-01 00:00:00

abstract：:Murine experimentation has provided many useful tools, including the ability to knockout or over-express genes and to perform experiments that are limited by ethical considerations. Over the past century, mice have imparted valuable insights into the biology of many systems, including human immunity. However, although...

journal_title：FEMS yeast research

authors： Marr KJ,Jones GJ,Mody CH

更新日期：2006-06-01 00:00:00

abstract：:Exposure of Hansenula polymorpha cells, grown in batch cultures on methanol at 37 degrees C, to a cold treatment (18 degrees C) is paralleled by a rapid degradation of peroxisomes present in these cells. Remarkably, the events accompanying organelle degradation at 18 degrees C are similar to those of selective glucose...

journal_title：FEMS yeast research

authors： Komduur JA,Bellu AR,Knoops K,van der Klei IJ,Veenhuis M

更新日期：2004-12-01 00:00:00

abstract：:Secreted lipases of Candida albicans are encoded by a gene family with at least 10 members (LIP1-LIP10). The expression pattern of this multigene family was investigated using reverse transcription polymerase chain reaction in experimental infections and in samples of patients suffering from oral candidosis. The findi...

journal_title：FEMS yeast research

authors： Stehr F,Felk A,Gácser A,Kretschmar M,Mähnss B,Neuber K,Hube B,Schäfer W

更新日期：2004-01-01 00:00:00

abstract：:Yeast isolates from orange fruit and juice in a spontaneous fermentation were identified and classified by two molecular techniques. The first was analysis of the restriction pattern generated from the polymerase chain reaction (PCR)-amplified 5.8S rRNA gene and the two internal transcribed spacers (ITS) using specifi...

journal_title：FEMS yeast research

authors： Las Heras-Vazquez FJ,Mingorance-Cazorla L,Clemente-Jimenez JM,Rodriguez-Vico F

更新日期：2003-03-01 00:00:00

abstract：:Extracellular proteins of Cryptococcus neoformans are involved in the pathogenesis of cryptococcosis, and some are immunoreactive antigens that may potentially serve as candidates for vaccine development. To further study the extracellular proteome of the human fungal pathogen Cry. neoformans, we conducted a proteomic...

journal_title：FEMS yeast research

authors： Eigenheer RA,Jin Lee Y,Blumwald E,Phinney BS,Gelli A

更新日期：2007-06-01 00:00:00

abstract：:Dysfunction or capacity shortage of the endoplasmic reticulum (ER) is cumulatively called ER stress and provokes the unfolded protein response (UPR). In various yeast species, the ER-located transmembrane protein Ire1 is activated upon ER stress and performs the splicing reaction of HAC1 mRNA, the mature form of which...

journal_title：FEMS yeast research

authors： Fauzee YNBM,Taniguchi N,Ishiwata-Kimata Y,Takagi H,Kimata Y

更新日期：2020-10-15 00:00:00

abstract：:Promoter of alcohol oxidase I (PAOX1) is the most efficient promoter involved in the regulation of recombinant protein expression in Pichia pastoris (P. pastoris). PAOX1 is tightly repressed by the presence of glycerol in the culture medium; thus, glycerol must be exhausted before methanol can be taken up by P. pastor...

journal_title：FEMS yeast research

authors： Zhan C,Wang S,Sun Y,Dai X,Liu X,Harvey L,McNeil B,Yang Y,Bai Z

更新日期：2016-06-01 00:00:00

abstract：:Every year more than 2 million tons of citric acid (CA) are produced around the world for industrial uses. Although initially extracted from citrus, the low profitability of the process and the increasing demand soon stimulated the search for more efficient methods to produce CA. Currently, most world CA demand (99%) ...

journal_title：FEMS yeast research

authors： Cavallo E,Charreau H,Cerrutti P,Foresti ML

更新日期：2017-12-01 00:00:00

abstract：:The roles of intracellular GTPase Arl1 and organellar cation/H(+) antiporters (Kha1 and Nhx1) in Saccharomyces cerevisiae tolerance to various stress factors were investigated and interesting new phenotypes of strains devoid of these proteins were found. The role of Arl1 GTPase in their tolerance to various cations is...

journal_title：FEMS yeast research

authors： Marešová L,Sychrová H

更新日期：2010-11-01 00:00:00

abstract：:Abstract Zinc is an essential micronutrient, and yet it can be toxic when present in excess. Zinc acquisition and distribution are dependent on tightly controlled transport of Zn(2+) ions. Schizosaccharomyces pombe represents a second eukaryotic model to study cellular metal homeostasis. In several ways its micronutri...

journal_title：FEMS yeast research

authors： Boch A,Trampczynska A,Simm C,Taudte N,Krämer U,Clemens S

更新日期：2008-09-01 00:00:00

abstract：:A robust cell factory that can tolerate combined inhibitory lignocellulosic compounds is essential for the cost-effective lignocellulose-based production of second-generation bioethanol and other bulk chemicals. Following high-throughput phenotyping of a yeast genomic overexpression library, we identified a Saccharomy...

journal_title：FEMS yeast research

authors： Unrean P,Gätgens J,Klein B,Noack S,Champreda V

更新日期：2018-12-01 00:00:00

abstract：:Here we report on the results of a survey of the yeast populations occurring on submerged leaves (alder, eucalyptus and oak) in a natural mountain stream, during different phases of their decomposition and through two consecutive years. Leaf litter mass loss, total yeast counts, Shannon-Weiner index (H'), yeast commun...

journal_title：FEMS yeast research

authors： Sampaio A,Sampaio JP,Leão C

更新日期：2007-06-01 00:00:00

abstract：:The ultimate goal of therapeutic vaccines is to activate and exploit the patient's own immune system to vigorously and dynamically seek and eradicate established malignant or virally infected cells. Therapeutic vaccines also offer the potential for preventing disease recurrence. Saccharomyces cerevisiae-based vaccines...

journal_title：FEMS yeast research

authors： Ardiani A,Higgins JP,Hodge JW

更新日期：2010-12-01 00:00:00

abstract：:The transcriptional regulator Pdr1 plays a positive role in regulating azole drug resistance in Candida glabrata. Previous studies have shown the importance of the carboxyl (C)-terminal sequence of Pdr1 in fulfilling its function, as this region mediates interactions between Pdr1 and the co-activator Gal11A and is cru...

journal_title：FEMS yeast research

authors： Tian Y,Gao N,Ni Q,Mao Y,Dong D,Huang X,Jiang C,Li Z,Zhang L,Wang X,Peng Y,Chen C

更新日期：2018-06-01 00:00:00