Abstract:
:Abstract Zinc is an essential micronutrient, and yet it can be toxic when present in excess. Zinc acquisition and distribution are dependent on tightly controlled transport of Zn(2+) ions. Schizosaccharomyces pombe represents a second eukaryotic model to study cellular metal homeostasis. In several ways its micronutrient metabolism is fundamentally different from Saccharomyces cerevisiae. We identified the first Zn(2+)-uptake system in S. pombe and named it SpZrt1. Knock-out strains for all three ZIP (Zrt, Irt-like protein) transporters in fission yeast were constructed. Only zrt1Delta cells were unable to grow at low Zn(2+) and showed reduced (65)Zn(2+) uptake. Elemental profiles revealed a strong decrease in zinc accumulation. Cd(2+) ions inhibited uptake but Fe(2+) or Mn(2+) did not. Both mRNA abundance and protein amount are tightly regulated. Zrt1 activity is rapidly shut down upon transfer of zinc-deficient cells to zinc-replete conditions. In cells lacking Zhf, a transporter mediating endoplasmic reticulum storage of zinc, this response is about 100-fold more sensitive. Thus, removal of excess of zinc from the cytosol is largely Zhf dependent. Moreover, cells deficient for both transporters are no longer able to adjust to changing external Zn(2+) concentrations. Optimal growth is restricted to a narrow range of Zn(2+) concentrations, illustrating the fine balance between micronutrient deficiency and toxicity.
journal_name
FEMS Yeast Resjournal_title
FEMS yeast researchauthors
Boch A,Trampczynska A,Simm C,Taudte N,Krämer U,Clemens Sdoi
10.1111/j.1567-1364.2008.00414.xsubject
Has Abstractpub_date
2008-09-01 00:00:00pages
883-96issue
6eissn
1567-1356issn
1567-1364pii
FYR414journal_volume
8pub_type
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pub_type: 杂志文章
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journal_title:FEMS yeast research
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pub_type: 杂志文章
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journal_title:FEMS yeast research
pub_type: 杂志文章
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更新日期:2015-03-01 00:00:00
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