Abstract：
:There is increasing evidence in the literature showing that fungal pathogens express biologically active ectoenzymes. The expression of surface phosphatases at the cell surface of Cryptococcus neoformans, the etiologic agent of cryptococcosis, was evaluated in the present study. Different isolates of C. neoformans express ectophosphatase activity, which is not influenced by capsule size or serotype. The cryptococcal enzyme is an acid phosphatase, inhibited by classic inhibitors of ectophosphatases, including ammonium molybdate and sodium salts of fluoride and orthovanadate. Only the inhibition of enzyme activity caused by sodium orthovanadate has been shown to be irreversible. The cryptococcal ectoenzyme is also inhibited by Zn2+ and inorganic phosphate, the final product of reactions catalyzed by phosphatases. The ectophosphatase from C. neoformans efficiently releases phosphate groups from different phosphorylated amino acids, giving a higher rate of phosphate removal when phosphothreonine is used as a substrate. Yeast cells with irreversibly inhibited ectophosphatases are less capable of adhering to animal epithelial cells than fungi fully expressing enzyme activity, suggesting that ectoenzyme expression can contribute to the pathogenesis of C. neoformans.
journal_name
FEMS Yeast Resjournal_title
FEMS yeast researchauthors
Collopy-Junior I,Esteves FF,Nimrichter L,Rodrigues ML,Alviano CS,Meyer-Fernandes JRdoi
10.1111/j.1567-1364.2006.00105.xsubject
Has Abstractpub_date
2006-11-01 00:00:00pages
1010-7issue
7eissn
1567-1356issn
1567-1364pii
FYR105journal_volume
6pub_type
杂志文章abstract：:Here we present evidence that Hsp150/Pir2, a member of the Pir family of cell wall proteins, can be extracted from the purified cell walls of Saccharomyces cerevisiae by treatment with beta-mercaptoethanol, demonstrating that at least part of this protein is attached to the cell wall through disulfide bridges. We also...
journal_title：FEMS yeast research
pub_type： 杂志文章
doi：10.1111/j.1567-1364.2001.tb00040.x
更新日期：2001-12-01 00:00:00
abstract：:The Tapirapé people of the Tapi'itãwa tribe of Brazil produce several fermented foods and beverages, one of which is called 'cauim'. This beverage usually makes up the main staple food for adults and children. Several substrates are used in its production, including cassava, rice, corn, maize and peanuts. A fermentati...
journal_title：FEMS yeast research
pub_type： 杂志文章
doi：10.1111/j.1567-1364.2007.00241.x
更新日期：2007-09-01 00:00:00
abstract：:Amylases are used in a variety of industries that have a specific need for alternative enzymes capable of hydrolysing raw starch. Five α-amylase and five glucoamylase-encoding genes were expressed in the Saccharomyces cerevisiae Y294 laboratory strain to select for recombinant strains that best hydrolysed raw corn sta...
journal_title：FEMS yeast research
pub_type： 杂志文章
doi：10.1093/femsyr/foy127
更新日期：2019-03-01 00:00:00
abstract：:Extracellular proteins of Cryptococcus neoformans are involved in the pathogenesis of cryptococcosis, and some are immunoreactive antigens that may potentially serve as candidates for vaccine development. To further study the extracellular proteome of the human fungal pathogen Cry. neoformans, we conducted a proteomic...
journal_title：FEMS yeast research
pub_type： 杂志文章
doi：10.1111/j.1567-1364.2006.00198.x
更新日期：2007-06-01 00:00:00
abstract：:Homologous recombination (HR) is an error-free DNA repair mechanism that maintains genome integrity by repairing double-strand breaks (DSBs). Defects in HR lead to genomic instability and are associated with cancer predisposition. A key step in HR is the formation of Rad51 nucleoprotein filaments which are responsible...
journal_title：FEMS yeast research
pub_type： 杂志文章
doi：10.1093/femsyr/fow073
更新日期：2016-09-01 00:00:00
abstract：:The histone acetyltransferase Sas2 is part of the SAS-I complex and acetylates lysine 16 of histone H4 (H4 K16Ac) in the genome of Saccharomyces cerevisiae. Sas2-mediated H4 K16Ac is strongest over the coding region of genes with low expression. However, it is unclear how Sas2-mediated acetylation is incorporated into...
journal_title：FEMS yeast research
pub_type： 杂志文章
doi：10.1093/femsyr/fov073
更新日期：2015-11-01 00:00:00
abstract：:A novel method dissecting the regulation of a cellular function into direct metabolic regulation and hierarchical (e.g., gene-expression) regulation is applied to yeast starved for nitrogen or carbon. Upon nitrogen starvation glucose influx is down-regulated hierarchically. Upon carbon starvation it is down-regulated ...
journal_title：FEMS yeast research
pub_type： 杂志文章
doi：10.1016/j.femsyr.2004.11.003
更新日期：2005-04-01 00:00:00
abstract：:Mitogen activated protein kinase (MAPK) cascades are signal transduction mechanisms present in eukaryotic cells that allow adaptation to environmental changes. MAPK activity is mainly regulated by dual phosphorylation in a TXY motif present in the kinase subdomain VIII as well as dephosphorylation by specific phosphat...
journal_title：FEMS yeast research
pub_type： 杂志文章
doi：10.1111/j.1567-1364.2009.00545.x
更新日期：2009-09-01 00:00:00
abstract：:Endosomal sorting complexes required for transport (ESCRTs) are involved in the formation of multivesicular bodies and sorting of targeted proteins to the yeast vacuole. The deletion of seven genes encoding components of the ESCRT machinery render Saccharomyces cerevisiae cells sensitive to high extracellular CaCl2 co...
journal_title：FEMS yeast research
pub_type： 杂志文章
doi：10.1093/femsyr/fov007
更新日期：2015-03-01 00:00:00
abstract：:Selenium (Se) is an essential element for most eukaryotic organisms, including humans. The balance between Se toxicity and its beneficial effects is very delicate. It has been demonstrated that a diet enriched with Se has cancer prevention potential in humans. The most popular commercial Se supplementation is selenize...
journal_title：FEMS yeast research
pub_type： 杂志文章
doi：10.1111/j.1567-1364.2011.00757.x
更新日期：2012-02-01 00:00:00
abstract：:Early screens in yeast for mutations exhibiting sensitivity to DNA damage identified nuclear pore components, but their role in DNA repair was not well understood. Over the last decade, studies have revealed that several types of persistent DNA lesions relocate to either the nuclear pore complex (NPC) or nuclear envel...
journal_title：FEMS yeast research
pub_type： 杂志文章,评审
doi：10.1093/femsyr/fow095
更新日期：2016-12-01 00:00:00
abstract：:The closely related yeasts Debaryomyces fabryi and Debaryomyces hansenii are excellent xylose consumers. We previously described the activity of a high-affinity xylose/H(+) symport from an industrial strain of D. hansenii subsequently reclassified as D. fabryi. We now report the identification of the gene encoding thi...
journal_title：FEMS yeast research
pub_type： 杂志文章
doi：10.1111/1567-1364.12061
更新日期：2013-11-01 00:00:00
abstract：:Saccharomyces cerevisiae yeast strains exhibit a huge genotypic and phenotypic diversity. Breeding strategies taking advantage of these characteristics would contribute greatly to improving industrial yeasts. Here we mapped and introgressed chromosomal regions controlling industrial yeast properties, such as hydrogen ...
journal_title：FEMS yeast research
pub_type： 杂志文章
doi：10.1111/j.1567-1364.2007.00281.x
更新日期：2007-12-01 00:00:00
abstract：:Candida glabrata is a second most common human opportunistic pathogen which causes superficial but also life-threatening systemic candidosis. According to the localisation of mannans and mannoproteins in the outermost layer of the cell wall, mannan detection could be one of the first steps in the cell recognition of C...
journal_title：FEMS yeast research
pub_type： 杂志文章
doi：10.1093/femsyr/foz009
更新日期：2019-03-01 00:00:00
abstract：:In previous communications the essential role of spermidine in Ustilago maydis was demonstrated by means of the disruption of the genes encoding ornithine decarboxylase (ODC) and spermidine synthase (SPE). However, the assignation of specific roles to each polyamine in different cellular functions was not possible bec...
journal_title：FEMS yeast research
pub_type： 杂志文章
doi：10.1111/j.1567-1364.2010.00675.x
更新日期：2010-11-01 00:00:00
abstract：:The yeast vacuolar membrane protein Ycf1p and its mammalian counterpart, MRP1, belong to the ABCC subfamily of ATP-binding cassette transporters. Genetic evidence suggests that the yeast casein kinase 2α, Cka1p, negatively regulates Ycf1p function via phosphorylation of Ser251 within the N-terminus. In this study, we ...
journal_title：FEMS yeast research
pub_type： 杂志文章
doi：10.1111/j.1567-1364.2010.00677.x
更新日期：2010-11-01 00:00:00
abstract：:Cdc37p, the p50 homolog of Saccharomyces cerevisiae, is an Hsp90 cochaperone involved in the targeting of protein kinases to Hsp90. Here we report a role for Cdc37p in osmoadaptive signalling in this yeast. The osmosensitive phenotype that is displayed by the cdc37-34 mutant strain appears not to be the consequence of...
journal_title：FEMS yeast research
pub_type： 杂志文章
doi：10.1111/j.1567-1364.2007.00237.x
更新日期：2007-09-01 00:00:00
abstract：:The essentiality of iodine for humans, especially in the early stages of life, is well recognized. The chemical forms of iodine in food supplements, infant formulae and iodated salt are either iodide (KI) or iodate (KIO(3)). Because there are no or rare data about iodine uptake by yeasts, we investigated the influence...
journal_title：FEMS yeast research
pub_type： 杂志文章
doi：10.1111/j.1567-1364.2008.00449.x
更新日期：2009-02-01 00:00:00
abstract：:We developed a method for the quantification of protein turnover rates using (13)C-labeled substrates combined with the analysis of the labeling patterns of proteinogenic amino acids. Using this method, the specific amino acid turnover rates between proteins and the pool of free amino acids were determined for eight d...
journal_title：FEMS yeast research
pub_type： 杂志文章
doi：10.1111/j.1567-1364.2012.00823.x
更新日期：2012-11-01 00:00:00
abstract：:Trk1 and Trk2 are the major K(+) transport systems in Schizosaccharomyces pombe. Both transporters individually seem to be able to cope with K(+) requirements of the cells under normal conditions, since only the double mutant shows defective K(+) transport and defective growth at limiting K(+) concentrations. We have ...
journal_title：FEMS yeast research
pub_type： 杂志文章
doi：10.1016/j.femsyr.2003.11.003
更新日期：2004-03-01 00:00:00
abstract：:Regulation of virulence factor production in the pathogen Cryptococcus neoformans remains to be fully illustrated. We present here a finding that a gene, encoding the glycolysis enzyme phosphoglucose isomerase (Pgi1), is critical for the biosynthesis of melanin and capsule, cell wall integrity and resistance to stress...
journal_title：FEMS yeast research
pub_type： 社论
doi：10.1093/femsyr/fov072
更新日期：2015-11-01 00:00:00
abstract：:In Kluyveromyces lactis, galactose transport has been thought to be mediated by the lactose permease encoded by LAC12. In fact, a lac12 mutant unable to grow on lactose did not grow on galactose either and showed low and uninducible galactose uptake activity. The existence of other galactose transport systems, at low ...
journal_title：FEMS yeast research
pub_type： 杂志文章
doi：10.1111/j.1567-1364.2006.00107.x
更新日期：2006-12-01 00:00:00
abstract：:In the macroscopic world, ecological interactions between multiple species of fauna and flora are recognised as major role-players in the evolution of any particular species. By comparison, research on ecological interactions as a driver of evolutionary adaptation in microbial ecosystems has been neglected. The evolut...
journal_title：FEMS yeast research
pub_type： 杂志文章,评审
doi：10.1093/femsyr/foz072
更新日期：2019-11-01 00:00:00
abstract：:Like bacteria, fungi growing in biofilms are often embedded in a so-called extracellular matrix (ECM), a complex and species-specific mixture of compounds secreted by cells in the biofilm. The precise physiological role of this ECM and its importance for the stress and drug resistance that is so characteristic of biof...
journal_title：FEMS yeast research
pub_type： 杂志文章
doi：10.1111/j.1567-1364.2009.00482.x
更新日期：2009-05-01 00:00:00
abstract：:Conversion of glucose to lactic acid is stoichiometrically equivalent to ethanol formation with respect to ATP formation from substrate-level phosphorylation, redox equivalents and product yield. However, anaerobic growth cannot be sustained in homolactate fermenting Saccharomyces cerevisiae. ATP-dependent export of t...
journal_title：FEMS yeast research
pub_type： 杂志文章
doi：10.1111/j.1567-1364.2009.00506.x
更新日期：2009-05-01 00:00:00
abstract：:We studied the effect of the loss of the Ser-Thr protein phosphatase Sit4, an important post-translational regulator, on the steady-state levels of the low-affinity glucose transporter Hxt1p and observed a delay in its appearance after high glucose induction, slow growth, and diminished glucose consumption. By analyzi...
journal_title：FEMS yeast research
pub_type： 杂志文章
doi：10.1111/j.1567-1364.2012.00839.x
更新日期：2012-12-01 00:00:00
abstract：:High-level production of bioethanol (140 g L(-1) in 45 h) in aerated fed-batch cultures of Saccharomyces cerevisiae was shown to be linked to the length of a production phase uncoupled to the growth. The induction of this phase was characterized by metabolic and morphologic changes reminiscent of those occurring in th...
journal_title：FEMS yeast research
pub_type： 杂志文章
doi：10.1111/j.1567-1364.2009.00553.x
更新日期：2009-12-01 00:00:00
abstract：:A salt-sensitive mutant of Kluyveromyces lactis was isolated that was unable to grow in high-salt media. This mutant was also respiratory-deficient and temperature-sensitive for growth. The mutation mapped in a single nuclear gene that is the ortholog of BCS1 of Saccharomyces cerevisiae. The BCS1 product is a mitochon...
journal_title：FEMS yeast research
pub_type： 杂志文章
doi：10.1111/j.1567-1364.2006.00148.x
更新日期：2007-03-01 00:00:00
abstract：:During their mitotic cycle, cylindrical fission yeast cells grow exclusively at their tips. Length growth starts at birth and halts at mitotic onset when the cells begin to prepare for division. While the growth pattern was initially considered to be exponential, during the last three decades an increasing amount of e...
journal_title：FEMS yeast research
pub_type： 杂志文章
doi：10.1111/1567-1364.12064
更新日期：2013-11-01 00:00:00
abstract：:Interspecies hybridization is an important evolutionary mechanism in yeasts. The genus Zygosaccharomyces in particular contains numerous hybrid strains and/or species. Here, we investigated the genome of Zygosaccharomyces strain MT15, an isolate from Maotai-flavor Chinese liquor fermentation. We found that it is an in...
journal_title：FEMS yeast research
pub_type： 杂志文章
doi：10.1093/femsyr/foy079
更新日期：2018-11-01 00:00:00
