Galactose transport in Kluyveromyces lactis: major role of the glucose permease Hgt1.


:In Kluyveromyces lactis, galactose transport has been thought to be mediated by the lactose permease encoded by LAC12. In fact, a lac12 mutant unable to grow on lactose did not grow on galactose either and showed low and uninducible galactose uptake activity. The existence of other galactose transport systems, at low and at high affinity, had, however, been hypothesized on the basis of galactose uptake kinetics studies. Here we confirmed the existence of a second galactose transporter and we isolated its structural gene. It turned out to be HGT1, previously identified as encoding the high-affinity glucose carrier. Analysis of galactose transporter mutants, hgt1 and lac12, and the double mutant hgt1lac12, suggested that Hgt1 was the high-affinity and Lac12 was the low-affinity galactose transporter. HGT1 expression was strongly induced by galactose and insensitive to glucose repression. This could explain the rapid adaptation to galactose observed in K. lactis after a shift from glucose to galactose medium.


FEMS Yeast Res


FEMS yeast research


Baruffini E,Goffrini P,Donnini C,Lodi T




Has Abstract


2006-12-01 00:00:00














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    abstract::We used multi-locus sequence typing (MLST) to investigate 35 yeast isolates representing the two genome-sequenced strains plus the type strain of Candida albicans, four isolates originally identified as Candida stellatoidea type I and 28 representing type strains of other species now regarded as synonymous with C. alb...

    journal_title:FEMS yeast research

    pub_type: 杂志文章


    authors: Jacobsen MD,Boekhout T,Odds FC

    更新日期:2008-08-01 00:00:00

  • Tipping the balance both ways: drug resistance and virulence in Candida glabrata.

    abstract::Among existing fungal pathogens, Candida glabrata is outstanding in its capacity to rapidly develop resistance to currently used antifungal agents. Resistance to the class of azoles, which are still widely used agents, varies in proportion (from 5 to 20%) depending on geographical area. Moreover, resistance to the cla...

    journal_title:FEMS yeast research

    pub_type: 杂志文章,评审


    authors: Vale-Silva LA,Sanglard D

    更新日期:2015-06-01 00:00:00

  • Metabolic response to MMS-mediated DNA damage in Saccharomyces cerevisiae is dependent on the glucose concentration in the medium.

    abstract::Maintenance and adaptation of energy metabolism could play an important role in the cellular ability to respond to DNA damage. A large number of studies suggest that the sensitivity of cells to oxidants and oxidative stress depends on the activity of cellular metabolism and is dependent on the glucose concentration. I...

    journal_title:FEMS yeast research

    pub_type: 杂志文章


    authors: Kitanovic A,Walther T,Loret MO,Holzwarth J,Kitanovic I,Bonowski F,Van Bui N,Francois JM,Wölfl S

    更新日期:2009-06-01 00:00:00

  • Vcx1 and ESCRT components regulate intracellular pH homeostasis in the response of yeast cells to calcium stress.

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    pub_type: 杂志文章


    authors: Papouskova K,Jiang L,Sychrova H

    更新日期:2015-03-01 00:00:00

  • Hierarchical and metabolic regulation of glucose influx in starved Saccharomyces cerevisiae.

    abstract::A novel method dissecting the regulation of a cellular function into direct metabolic regulation and hierarchical (e.g., gene-expression) regulation is applied to yeast starved for nitrogen or carbon. Upon nitrogen starvation glucose influx is down-regulated hierarchically. Upon carbon starvation it is down-regulated ...

    journal_title:FEMS yeast research

    pub_type: 杂志文章


    authors: Rossell S,van der Weijden CC,Kruckeberg AL,Bakker BM,Westerhoff HV

    更新日期:2005-04-01 00:00:00

  • Dynamic 13C-labeling experiments prove important differences in protein turnover rate between two Saccharomyces cerevisiae strains.

    abstract::We developed a method for the quantification of protein turnover rates using (13)C-labeled substrates combined with the analysis of the labeling patterns of proteinogenic amino acids. Using this method, the specific amino acid turnover rates between proteins and the pool of free amino acids were determined for eight d...

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    authors: Hong KK,Hou J,Shoaie S,Nielsen J,Bordel S

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  • Characterization and functional analysis of the beta-1,3-glucanosyltransferase 3 of the human pathogenic fungus Paracoccidioides brasiliensis.

    abstract::The fungus Paracoccidioides brasiliensis causes paracoccidioidomycosis, a systemic granulomatous mycosis prevalent in Latin America. In an effort to elucidate the molecular mechanisms involved in fungus cell wall assembly and morphogenesis, beta-1,3-glucanosyltransferase 3 (PbGel3p) is presented here. PbGel3p presente...

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    authors: Castro Nda S,de Castro KP,Orlandi I,Feitosa Ldos S,Rosa e Silva LK,Vainstein MH,Báo SN,Vai M,Soares CM

    更新日期:2009-02-01 00:00:00

  • On the role of Trk1 and Trk2 in Schizosaccharomyces pombe under different ion stress conditions.

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    journal_title:FEMS yeast research

    pub_type: 杂志文章


    authors: Calero F,Montiel V,Caracuel Z,Cabello-Hurtado F,Ramos J

    更新日期:2004-03-01 00:00:00

  • The F1-ATPase inhibitor Inh1 (IF1) affects suppression of mtDNA loss-lethality in Kluyveromyces lactis.

    abstract::Loss of mtDNA by the petite-negative yeast Kluyveromyces lactis is lethal (rho(o)-lethality). However, mutations in the alpha, beta and gamma subunits of F(1)-ATPase can suppress lethality by increasing intramitochondrial hydrolysis of ATP. Increased hydrolysis of ATP can also occur on inactivation of Inh1, the natura...

    journal_title:FEMS yeast research

    pub_type: 杂志文章


    authors: Clark-Walker GD

    更新日期:2007-08-01 00:00:00

  • Detailed search for protein kinase(s) involved in plasma membrane H+-ATPase activity regulation of yeast cells.

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    pub_type: 杂志文章


    authors: Pereira RR,Castanheira D,Teixeira JA,Bouillet LE,Ribeiro EM,Trópia MM,Alvarez F,Correa LF,Mota BE,Conceição LE,Castro IM,Brandão RL

    更新日期:2015-03-01 00:00:00

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    authors: Mapelli V,Hillestrøm PR,Patil K,Larsen EH,Olsson L

    更新日期:2012-02-01 00:00:00

  • Cbc2p, Upf3p and eIF4G are components of the DRN (Degradation of mRNA in the Nucleus) in Saccharomyces cerevisiae.

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    authors: Das S,Saha U,Das B

    更新日期:2014-09-01 00:00:00

  • Schizosaccharomyces cryophilus sp. nov., a new species of fission yeast.

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    pub_type: 杂志文章


    authors: Helston RM,Box JA,Tang W,Baumann P

    更新日期:2010-09-01 00:00:00

  • Yeast diversity in rice-cassava fermentations produced by the indigenous Tapirapé people of Brazil.

    abstract::The Tapirapé people of the Tapi'itãwa tribe of Brazil produce several fermented foods and beverages, one of which is called 'cauim'. This beverage usually makes up the main staple food for adults and children. Several substrates are used in its production, including cassava, rice, corn, maize and peanuts. A fermentati...

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    pub_type: 杂志文章


    authors: Schwan RF,Almeida EG,Souza-Dias MA,Jespersen L

    更新日期:2007-09-01 00:00:00

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    authors: Klompmaker SH,Kilic A,Baerends RJ,Veenhuis M,van der Klei IJ

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    pub_type: 杂志文章,评审


    authors: Sipiczki M

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    abstract::Viruses are found in almost all organisms and physical habitats. One interesting example is the yeast viral 'killer system'. The virus provides the host with a toxin directed against strains that do not carry it, while the yeast cell enables its propagation. Although yeast viruses are believed to be common, they have ...

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    pub_type: 杂志文章


    authors: Pieczynska MD,de Visser JA,Korona R

    更新日期:2013-12-01 00:00:00

  • Suppression of Ycf1p function by Cka1p-dependent phosphorylation is attenuated in response to salt stress.

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    pub_type: 杂志文章


    authors: Pickin KA,Ezenwajiaku N,Overcash H,Sethi M,Knecht MR,Paumi CM

    更新日期:2010-11-01 00:00:00

  • Ubiquinone biosynthesis in Saccharomyces cerevisiae: the molecular organization of O-methylase Coq3p depends on Abc1p/Coq8p.

    abstract::Coenzyme Q is a redox-active lipid that functions as an electron carrier in the mitochondrial respiratory chain. Q-biosynthesis in Saccharomyces cerevisiae requires at least nine proteins (Coq1p-Coq9p). The molecular function of Coq8p is still unknown; however, lack of Q and the concomitant accumulation of the interme...

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    pub_type: 杂志文章


    authors: Tauche A,Krause-Buchholz U,Rödel G

    更新日期:2008-12-01 00:00:00

  • Genetic interactions among the Arl1 GTPase and intracellular Na(+) /H(+) antiporters in pH homeostasis and cation detoxification.

    abstract::The roles of intracellular GTPase Arl1 and organellar cation/H(+) antiporters (Kha1 and Nhx1) in Saccharomyces cerevisiae tolerance to various stress factors were investigated and interesting new phenotypes of strains devoid of these proteins were found. The role of Arl1 GTPase in their tolerance to various cations is...

    journal_title:FEMS yeast research

    pub_type: 杂志文章


    authors: Marešová L,Sychrová H

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  • Isolation of two cell populations from yeast during high-level alcoholic fermentation that resemble quiescent and nonquiescent cells from the stationary phase on glucose.

    abstract::High-level production of bioethanol (140 g L(-1) in 45 h) in aerated fed-batch cultures of Saccharomyces cerevisiae was shown to be linked to the length of a production phase uncoupled to the growth. The induction of this phase was characterized by metabolic and morphologic changes reminiscent of those occurring in th...

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    pub_type: 杂志文章


    authors: Benbadis L,Cot M,Rigoulet M,Francois J

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  • Loss of Zhf and the tightly regulated zinc-uptake system SpZrt1 in Schizosaccharomyces pombe reveals the delicacy of cellular zinc balance.

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    journal_title:FEMS yeast research

    pub_type: 杂志文章


    authors: Boch A,Trampczynska A,Simm C,Taudte N,Krämer U,Clemens S

    更新日期:2008-09-01 00:00:00

  • Yeast mitochondria: an overview of mitochondrial biology and the potential of mitochondrial systems biology.

    abstract::Mitochondria are dynamic organelles of endosymbiotic origin that are essential components of eukaryal cells. They contain their own genetic machinery, have multicopy genomes and like their bacterial ancestors they consist of two membranes. However, the majority of the ancestral genome has been lost or transferred to t...

    journal_title:FEMS yeast research

    pub_type: 杂志文章,评审


    authors: Malina C,Larsson C,Nielsen J

    更新日期:2018-08-01 00:00:00

  • Glucose-induced and nitrogen-starvation-induced peroxisome degradation are distinct processes in Hansenula polymorpha that involve both common and unique genes.

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    pub_type: 杂志文章


    authors: Bellu AR,Kram AM,Kiel JA,Veenhuis M,van der Klei IJ

    更新日期:2001-04-01 00:00:00

  • Peer pressure: evolutionary responses to biotic pressures in wine yeasts.

    abstract::In the macroscopic world, ecological interactions between multiple species of fauna and flora are recognised as major role-players in the evolution of any particular species. By comparison, research on ecological interactions as a driver of evolutionary adaptation in microbial ecosystems has been neglected. The evolut...

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    pub_type: 杂志文章,评审


    authors: Conacher CG,Rossouw D,Bauer FFB

    更新日期:2019-11-01 00:00:00

  • A novel assay for replicative lifespan in Saccharomyces cerevisiae.

    abstract::The replicative lifespan of Saccharomyces cerevisiae is determined by both genetic and environmental factors. Many of the same factors determine the lifespan of metazoan animals. The lack of fast and reliable lifespan assays has limited the pace of yeast aging research. In this study we describe a novel strategy for a...

    journal_title:FEMS yeast research

    pub_type: 杂志文章


    authors: Jarolim S,Millen J,Heeren G,Laun P,Goldfarb DS,Breitenbach M

    更新日期:2004-11-01 00:00:00

  • Microbial alcohol-conferred hemolysis is a late response to alcohol stress.

    abstract::We have reported previously that growth on alcohol vapors confers hemolytic properties on certain yeast species and strains ['microbial alcohol-conferred hemolysis' (MACH)]. In a recent study, we analyzed the genetic basis of MACH in Saccharomyces cerevisiae using the EUROSCARF mutant collection. The data suggested th...

    journal_title:FEMS yeast research

    pub_type: 杂志文章


    authors: Shuster A,Korem M,Jacob-Hirsch J,Amariglio N,Rechavi G,Rosenberg M

    更新日期:2011-06-01 00:00:00

  • Phenotypic evaluation and characterization of 21 industrial Saccharomyces cerevisiae yeast strains.

    abstract::Microorganisms have been studied and used extensively to produce value-added fuels and chemicals. Yeasts, specifically Saccharomyces cerevisiae, receive industrial attention because of their well-known ability to ferment glucose and produce ethanol. Thousands of natural or genetically modified S. cerevisiae have been ...

    journal_title:FEMS yeast research

    pub_type: 杂志文章


    authors: Kong II,Turner TL,Kim H,Kim SR,Jin YS

    更新日期:2018-02-01 00:00:00

  • Cell length growth in fission yeast: an analysis of its bilinear character and the nature of its rate change transition.

    abstract::During their mitotic cycle, cylindrical fission yeast cells grow exclusively at their tips. Length growth starts at birth and halts at mitotic onset when the cells begin to prepare for division. While the growth pattern was initially considered to be exponential, during the last three decades an increasing amount of e...

    journal_title:FEMS yeast research

    pub_type: 杂志文章


    authors: Horváth A,Rácz-Mónus A,Buchwald P,Sveiczer Á

    更新日期:2013-11-01 00:00:00

  • Galactose transporters discriminate steric anomers at the cell surface in yeast.

    abstract::Aldose-1-epimerase or mutarotase (EC catalyzes interconversion of alpha/beta-anomers of aldoses, such as glucose and galactose, and is distributed in a wide variety of organisms from bacteria to humans. Nevertheless, the physiological role of this enzyme has been elusive in most cases, because the alpha-form ...

    journal_title:FEMS yeast research

    pub_type: 杂志文章


    authors: Fukasawa T,Sakurai H,Nogi Y,Baruffini E

    更新日期:2009-08-01 00:00:00