The Pichia pastoris transmembrane protein GT1 is a glycerol transporter and relieves the repression of glycerol on AOX1 expression.

abstract：:Many of the difficulties of studying evolution in action can be surmounted using populations of microorganisms, such as yeast. A readily manipulated sexual system and an increasingly sophisticated array of molecular and genomic tools uniquely qualify Saccharomyces cerevisiae as an experimental subject. This minireview...

journal_title：FEMS yeast research

authors： Zeyl C

更新日期：2006-08-01 00:00:00

abstract：:The rDNA sequencing data obtained during the last 5 years in several laboratories clearly demonstrate that within the heterogeneous genus Kluyveromyces there is a group of highly related species, which we refer to as the genus Zygofabospora Kudriavzev 1960 emend. G. Naumov 2002. This genus includes four hybridizing sp...

journal_title：FEMS yeast research

authors： Naumov GI,Naumova ES

更新日期：2002-03-01 00:00:00

abstract：:Molecular tools for the production of heterologous proteins and metabolic engineering applications of the non-conventional yeast Zygosaccharomyces bailii were developed. The combination of Z. bailii's resistance to relatively high temperature, osmotic pressure and low pH values, with a high specific growth rate render...

journal_title：FEMS yeast research

authors： Branduardi P,Valli M,Brambilla L,Sauer M,Alberghina L,Porro D

更新日期：2004-01-01 00:00:00

abstract：:Heat shock response (HSR) is an important element of cellular homeostasis. In yeast, HSR comprises of the heat shock proteins (Hsps) and the osmolytes trehalose and glycerol. The respective roles of trehalose and Hsp104 in regulating protein aggregation remain ambiguous. We report that trehalose and Hsp104 are importa...

journal_title：FEMS yeast research

authors： Sethi R,Iyer SS,Das E,Roy I

更新日期：2018-09-01 00:00:00

abstract：:Each time a cell duplicates, the whole genome must be accurately copied and distributed. The enormous amount of DNA in eukaryotic cells requires a high level of coordination between polymerases and other DNA and chromatin-interacting proteins to ensure timely and accurate DNA replication and chromatin formation. PCNA ...

journal_title：FEMS yeast research

authors： Kupiec M

更新日期：2016-11-01 00:00:00

abstract：:Murine experimentation has provided many useful tools, including the ability to knockout or over-express genes and to perform experiments that are limited by ethical considerations. Over the past century, mice have imparted valuable insights into the biology of many systems, including human immunity. However, although...

journal_title：FEMS yeast research

authors： Marr KJ,Jones GJ,Mody CH

更新日期：2006-06-01 00:00:00

abstract：:The transcriptional regulator Pdr1 plays a positive role in regulating azole drug resistance in Candida glabrata. Previous studies have shown the importance of the carboxyl (C)-terminal sequence of Pdr1 in fulfilling its function, as this region mediates interactions between Pdr1 and the co-activator Gal11A and is cru...

journal_title：FEMS yeast research

authors： Tian Y,Gao N,Ni Q,Mao Y,Dong D,Huang X,Jiang C,Li Z,Zhang L,Wang X,Peng Y,Chen C

更新日期：2018-06-01 00:00:00

abstract：:Xylose fermentation is a rare trait that is immensely important to the cellulosic biofuel industry, and Candida tenuis is one of the few yeasts that has been reported with this trait. Here we report the isolation of two strains representing a candidate sister species to C. tenuis. Integrated analysis of genome sequenc...

journal_title：FEMS yeast research

authors： Haase MAB,Kominek J,Langdon QK,Kurtzman CP,Hittinger CT

更新日期：2017-05-01 00:00:00

abstract：:The progenitor of the Dekkera/Brettanomyces clade separated from the Saccharomyces/Kluyveromyces clade over 200 million years ago. However, within both clades, several lineages developed similar physiological traits. Both Saccharomyces cerevisiae and Dekkera bruxellensis are facultative anaerobes; in the presence of e...

journal_title：FEMS yeast research

authors： Procházka E,Poláková S,Piskur J,Sulo P

更新日期：2010-08-01 00:00:00

abstract：:Current paradigms assume that gas bubbles cannot be formed within yeasts although these workhorses of the baking and brewing industries vigorously produce and release CO(2) gas. We show that yeasts produce gas bubbles that fill a significant part of the cell. The missing link between intracellular CO(2) production b...

journal_title：FEMS yeast research

authors： Swart CW,Dithebe K,Pohl CH,Swart HC,Coetsee E,van Wyk PW,Swarts JC,Lodolo EJ,Kock JL

更新日期：2012-11-01 00:00:00

abstract：:Using modern peptide analytical MS technology ('Peptidomics'), it is possible to analyze yeast α-pheromone both qualitatively and semi-quantitatively directly from conditioned cell culture media. MS/MS analysis shows both forms of α-pheromone (MFα and MFα') detectable and identifiable straight from WT supernatants. In...

journal_title：FEMS yeast research

authors： Bener Aksam E,Pinkse MW,Verhaert PD

更新日期：2013-05-01 00:00:00

abstract：:Cell volume is an important parameter for modelling cellular processes. Temperature-induced variability of cellular size, volume, intracellular granularity, a fraction of budding cells of yeast Saccharomyces cerevisiae CEN.PK 113-7D (in anaerobic glucose unlimited batch cultures) were measured by flow cytometry and ma...

journal_title：FEMS yeast research

authors： Zakhartsev M,Reuss M

更新日期：2018-09-01 00:00:00

abstract：:In vitro evaluation of specific anti-Candida albicans sera antibodies based on synthetically prepared complement receptor 3-related protein (CR3-RP) mimicking the structure of native complement receptor 3 in a cohort of 72 patients with atopy and recurrent Candida vulvovaginitis (RVC) revealed effective humoral respon...

journal_title：FEMS yeast research

authors： Paulovičová E,Bujdáková H,Chupáčová J,Paulovičová L,Kertys P,Hrubiško M

更新日期：2015-03-01 00:00:00

abstract：:The catabolism of methionine to methionol and methanethiol in Saccharomyces cerevisiae was studied using (13)C NMR spectroscopy, GC-MS, enzyme assays and a number of mutants. Methionine is first transaminated to alpha-keto-gamma-(methylthio)butyrate. Methionol is formed by a decarboxylation reaction, which yields meth...

journal_title：FEMS yeast research

authors： Perpète P,Duthoit O,De Maeyer S,Imray L,Lawton AI,Stavropoulos KE,Gitonga VW,Hewlins MJ,Dickinson JR

更新日期：2006-01-01 00:00:00

abstract：:To analyse the reliability and accuracy of genotype analysis with high-density oligonucleotide microarrays, this method and other experimental approaches were used to analyse genomic DNA of two popular Saccharomyces cerevisiae laboratory strains. S288C was used for systematic sequencing of 'the' S. cerevisiae genome; ...

journal_title：FEMS yeast research

authors： Daran-Lapujade P,Daran JM,Kötter P,Petit T,Piper MD,Pronk JT

更新日期：2003-12-01 00:00:00

abstract：:The fungal cell wall is a coherent structure formed by microfibrillar polysaccharides and amorphous material made of other polysaccharides and proteins. We performed a phylogenetic analysis of covalent proteins and enzymes that synthesize fungal wall polysaccharides to determine the possible evolution of the wall stru...

journal_title：FEMS yeast research

authors： Ruiz-Herrera J,Ortiz-Castellanos L

更新日期：2010-05-01 00:00:00

abstract：:Candida lipolytica and Candida rugosa were isolated from blood samples from a patient with chronic myeloid leukemia (31 years old) and a patient with sickle cell disease (1-year-old), respectively. Isolates were grown for 48 h at 37 degrees C in either Sabouraud or tryptone soy broth (TSB). Peptidases were tested for ...

journal_title：FEMS yeast research

authors： De Melo AC,Dornelas-Ribeiro M,De Souza EP,Macrae A,Fracalanzza SE,Vermelho AB

更新日期：2007-09-01 00:00:00

abstract：:Saccharomyces cerevisiae shows similar responses to heat shock and ethanol stress. Cells treated with severe ethanol stress activate the transcription of HSP genes and cause the aggregation of Hsp104-GFP, implying that severe ethanol stress as well as heat shock causes the accumulation of denatured proteins in yeast c...

journal_title：FEMS yeast research

authors： Kato S,Yoshida M,Izawa S

更新日期：2019-12-01 00:00:00

abstract：:The yeast Pichia anomala J121 prevents mold spoilage and enhances preservation of moist grain in malfunctioning storage systems. Development of P. anomala J121 as a biocontrol agent requires in-depth knowledge about its physiology. P. anomala J121 grew under strictly anaerobic conditions, at temperatures between 3 deg...

journal_title：FEMS yeast research

authors： Fredlund E,Druvefors U,Boysen ME,Lingsten KJ,Schnürer J

更新日期：2002-08-01 00:00:00

abstract：:A robust cell factory that can tolerate combined inhibitory lignocellulosic compounds is essential for the cost-effective lignocellulose-based production of second-generation bioethanol and other bulk chemicals. Following high-throughput phenotyping of a yeast genomic overexpression library, we identified a Saccharomy...

journal_title：FEMS yeast research

authors： Unrean P,Gätgens J,Klein B,Noack S,Champreda V

更新日期：2018-12-01 00:00:00

abstract：:Previous work has shown that the synthetic lethality of the slt2Δrim101Δ mutant results from a combination of factors, including improper functioning of the septum assembly machinery. Here, we identify new multicopy suppressors of this lethality including Kss1, Pcl1 and Sph1, none of which seems to be linked to the up...

journal_title：FEMS yeast research

authors： Pérez J,Arcones I,Gómez A,Casquero V,Roncero C

更新日期：2016-09-01 00:00:00

abstract：:During their mitotic cycle, cylindrical fission yeast cells grow exclusively at their tips. Length growth starts at birth and halts at mitotic onset when the cells begin to prepare for division. While the growth pattern was initially considered to be exponential, during the last three decades an increasing amount of e...

journal_title：FEMS yeast research

authors： Horváth A,Rácz-Mónus A,Buchwald P,Sveiczer Á

更新日期：2013-11-01 00:00:00

abstract：:In Saccharomyces cerevisiae, the cell integrity pathway plays a role in the oxidative stress response. In this study, we show that the Pkc1 protein mediates oxidative signalling by helping to downregulate ribosomal gene expression when cells are exposed to hydrogen peroxide. An active actin cytoskeleton is required fo...

journal_title：FEMS yeast research

authors： Mitjana FV,Petkova MI,Pujol-Carrion N,de la Torre-Ruiz MA

更新日期：2011-12-01 00:00:00

abstract：:Mitochondria are dynamic organelles of endosymbiotic origin that are essential components of eukaryal cells. They contain their own genetic machinery, have multicopy genomes and like their bacterial ancestors they consist of two membranes. However, the majority of the ancestral genome has been lost or transferred to t...

journal_title：FEMS yeast research

authors： Malina C,Larsson C,Nielsen J

更新日期：2018-08-01 00:00:00

abstract：:The Saccharomyces cerevisiae allantoate/ureidosuccinate permease gene (DAL5) is often used as a reporter in studies of the Tor1/2 protein kinases which are specifically inhibited by the clinically important immunosuppressant and anti-neoplastic drug, rapamycin. To date, only a single type of cis-acting element has bee...

journal_title：FEMS yeast research

authors： Rai R,Daugherty JR,Tate JJ,Buford TD,Cooper TG

更新日期：2004-10-01 00:00:00

abstract：:We studied the effect of the loss of the Ser-Thr protein phosphatase Sit4, an important post-translational regulator, on the steady-state levels of the low-affinity glucose transporter Hxt1p and observed a delay in its appearance after high glucose induction, slow growth, and diminished glucose consumption. By analyzi...

journal_title：FEMS yeast research

authors： Souza AA,Miranda MN,da Silva SF,Bozaquel-Morais B,Masuda CA,Ghislain M,Montero-Lomelí M

更新日期：2012-12-01 00:00:00

abstract：:Genes of the post-squalene ergosterol biosynthetic pathway in Saccharomyces cerevisiae have been overexpressed in a systematic approach with the aim to construct yeast strains that produce high amounts of sterols from a squalene-accumulating strain. This strain had previously been deregulated by overexpressing a trunc...

journal_title：FEMS yeast research

authors： Veen M,Stahl U,Lang C

更新日期：2003-10-01 00:00:00

abstract：:This study displays a screening using yeast strains deficient in protein kinases known to exist in Saccharomyces cerevisiae. From 95 viable single mutants, 20 mutants appear to be affected in the glucose-induced extracellular acidification. The mutants that are unaffected in calcium signaling were tested for their sen...

journal_title：FEMS yeast research

authors： Pereira RR,Castanheira D,Teixeira JA,Bouillet LE,Ribeiro EM,Trópia MM,Alvarez F,Correa LF,Mota BE,Conceição LE,Castro IM,Brandão RL

更新日期：2015-03-01 00:00:00

abstract：:Regulation of virulence factor production in the pathogen Cryptococcus neoformans remains to be fully illustrated. We present here a finding that a gene, encoding the glycolysis enzyme phosphoglucose isomerase (Pgi1), is critical for the biosynthesis of melanin and capsule, cell wall integrity and resistance to stress...

journal_title：FEMS yeast research

authors： Zhang P,Wei D,Li Z,Sun Z,Pan J,Zhu X

更新日期：2015-11-01 00:00:00

abstract：:In the macroscopic world, ecological interactions between multiple species of fauna and flora are recognised as major role-players in the evolution of any particular species. By comparison, research on ecological interactions as a driver of evolutionary adaptation in microbial ecosystems has been neglected. The evolut...

journal_title：FEMS yeast research

authors： Conacher CG,Rossouw D,Bauer FFB

更新日期：2019-11-01 00:00:00