Comparative genotyping of the Saccharomyces cerevisiae laboratory strains S288C and CEN.PK113-7D using oligonucleotide microarrays.

abstract：:The mechanistic details of mtDNA maintenance in petite-negative yeasts have remained largely unexplored. We report here that the DNA helicase Hmi1p plays a crucial role in mtDNA stability in Candida albicans. Like its counterpart in Saccharomyces cerevisiae, Hmi1p in C. albicans (CaHmi1p) contains a C-terminal mitocho...

journal_title：FEMS yeast research

authors： Jõers P,Gerhold JM,Sedman T,Kuusk S,Sedman J

更新日期：2007-01-01 00:00:00

abstract：:Killer-toxin complexes produced by Kluyveromyces lactis and Pichia acaciae inhibit cell proliferation of Saccharomyces cerevisiae. Analysis of their actions in haploid MATalpha cells revealed that introduction of the opposite mating-type locus (MATa) significantly suppressed antizymosis. Together with resistance expre...

journal_title：FEMS yeast research

authors： Klassen R,Jablonowski D,Stark MJ,Schaffrath R,Meinhardt F

更新日期：2006-05-01 00:00:00

abstract：:In the macroscopic world, ecological interactions between multiple species of fauna and flora are recognised as major role-players in the evolution of any particular species. By comparison, research on ecological interactions as a driver of evolutionary adaptation in microbial ecosystems has been neglected. The evolut...

journal_title：FEMS yeast research

authors： Conacher CG,Rossouw D,Bauer FFB

更新日期：2019-11-01 00:00:00

abstract：:A robust cell factory that can tolerate combined inhibitory lignocellulosic compounds is essential for the cost-effective lignocellulose-based production of second-generation bioethanol and other bulk chemicals. Following high-throughput phenotyping of a yeast genomic overexpression library, we identified a Saccharomy...

journal_title：FEMS yeast research

authors： Unrean P,Gätgens J,Klein B,Noack S,Champreda V

更新日期：2018-12-01 00:00:00

abstract：:In Saccharomyces cerevisiae, the cell integrity pathway plays a role in the oxidative stress response. In this study, we show that the Pkc1 protein mediates oxidative signalling by helping to downregulate ribosomal gene expression when cells are exposed to hydrogen peroxide. An active actin cytoskeleton is required fo...

journal_title：FEMS yeast research

authors： Mitjana FV,Petkova MI,Pujol-Carrion N,de la Torre-Ruiz MA

更新日期：2011-12-01 00:00:00

abstract：:We used multi-locus sequence typing (MLST) to investigate 35 yeast isolates representing the two genome-sequenced strains plus the type strain of Candida albicans, four isolates originally identified as Candida stellatoidea type I and 28 representing type strains of other species now regarded as synonymous with C. alb...

journal_title：FEMS yeast research

authors： Jacobsen MD,Boekhout T,Odds FC

更新日期：2008-08-01 00:00:00

abstract：:Early screens in yeast for mutations exhibiting sensitivity to DNA damage identified nuclear pore components, but their role in DNA repair was not well understood. Over the last decade, studies have revealed that several types of persistent DNA lesions relocate to either the nuclear pore complex (NPC) or nuclear envel...

journal_title：FEMS yeast research

authors： Freudenreich CH,Su XA

更新日期：2016-12-01 00:00:00

abstract：:The expression of the transcription regulator Pdr1p and its target genes PDR5 and TPO1 is required for Saccharomyces cerevisiae adaptation and resistance to artesunate, a promising antimalarial drug, also active against tumour cells and viruses. PDR5 and TPO1 encode plasma membrane multidrug transporters of the ATP-bi...

journal_title：FEMS yeast research

authors： Alenquer M,Tenreiro S,Sá-Correia I

更新日期：2006-12-01 00:00:00

abstract：:In yeast, rRNA genes can be detected with the FISH technique using rRNA gene probes. This technique yields reliable, reproducible and precise results, but is time-consuming. Here, the primed in situ DNA synthesis (PRINS) procedure has been optimized for rapid detection of yeast rRNA genes. PRINS, which is as sensitive...

journal_title：FEMS yeast research

authors： Wnuk M,Lewinska A,Bugno M,Bartosz G,Slota E

更新日期：2009-06-01 00:00:00

abstract：:Maf1 is a repressor of RNA polymerase (Pol) III transcription for tRNA. Nutrient deprivation and environmental stress repress Pol III transcription through Maf1 in Saccharomyces cerevisiae. The sole Candida albicans homolog CaMaf1 is a protein of 380 amino acids with conserved domains and motifs of the eukaryotic Maf1...

journal_title：FEMS yeast research

authors： Asghar F,Yan H,Jiang L

更新日期：2018-09-01 00:00:00

abstract：:d-Amino acid oxidase (DAO) is an important flavo-enzyme that catalyzes the oxidative deamination of d-amino acids into the corresponding alpha-keto acid, ammonia and H(2)O(2). We identified two amino acid oxidases in the methylotrophic yeast Pichia pastoris: Dao1p, which preferentially uses d-alanine as a substrate, a...

journal_title：FEMS yeast research

authors： Klompmaker SH,Kilic A,Baerends RJ,Veenhuis M,van der Klei IJ

更新日期：2010-09-01 00:00:00

abstract：:Heat shock response (HSR) is an important element of cellular homeostasis. In yeast, HSR comprises of the heat shock proteins (Hsps) and the osmolytes trehalose and glycerol. The respective roles of trehalose and Hsp104 in regulating protein aggregation remain ambiguous. We report that trehalose and Hsp104 are importa...

journal_title：FEMS yeast research

authors： Sethi R,Iyer SS,Das E,Roy I

更新日期：2018-09-01 00:00:00

abstract：:Zinc is a crucial mineral for all organisms as it is an essential cofactor for the proper function of a plethora of proteins and depletion of zinc causes oxidative stress. Glutathione is the major redox buffering agent in the cell and therefore important for mitigation of the adverse effects of oxidative stress. In ma...

journal_title：FEMS yeast research

authors： Steiger MG,Patzschke A,Holz C,Lang C,Causon T,Hann S,Mattanovich D,Sauer M

更新日期：2017-06-01 00:00:00

abstract：:We developed a method for the quantification of protein turnover rates using (13)C-labeled substrates combined with the analysis of the labeling patterns of proteinogenic amino acids. Using this method, the specific amino acid turnover rates between proteins and the pool of free amino acids were determined for eight d...

journal_title：FEMS yeast research

authors： Hong KK,Hou J,Shoaie S,Nielsen J,Bordel S

更新日期：2012-11-01 00:00:00

abstract：:Microorganisms have been studied and used extensively to produce value-added fuels and chemicals. Yeasts, specifically Saccharomyces cerevisiae, receive industrial attention because of their well-known ability to ferment glucose and produce ethanol. Thousands of natural or genetically modified S. cerevisiae have been ...

journal_title：FEMS yeast research

authors： Kong II,Turner TL,Kim H,Kim SR,Jin YS

更新日期：2018-02-01 00:00:00

abstract：:Brettanomyces bruxellensis is usually considered a spoilage microorganism, responsible for significant economic losses during the production of fermented beverages such as wine, beer and cider, though for some styles of beer its influence is essential. In recent years, the competitiveness of this yeast in bioethanol p...

journal_title：FEMS yeast research

authors： Varela C,Lleixà J,Curtin C,Borneman A

更新日期：2018-11-01 00:00:00

abstract：:The yeast glucose transporters Hxt1, Hxt2, Hxt3, Hxt4, Hxt6, Hxt7 and Gal2, individually expressed in an hxt1-7 null mutant strain, demonstrate the phenomenon of countertransport. Thus, these transporters, which are the most important glucose transporters in Saccharomyces cerevisiae, are facilitated diffusion transpor...

journal_title：FEMS yeast research

authors： Maier A,Völker B,Boles E,Fuhrmann GF

更新日期：2002-12-01 00:00:00

abstract：:Like bacteria, fungi growing in biofilms are often embedded in a so-called extracellular matrix (ECM), a complex and species-specific mixture of compounds secreted by cells in the biofilm. The precise physiological role of this ECM and its importance for the stress and drug resistance that is so characteristic of biof...

journal_title：FEMS yeast research

authors： Beauvais A,Loussert C,Prevost MC,Verstrepen K,Latgé JP

更新日期：2009-05-01 00:00:00

abstract：:Schizosaccharomyces pombe Cap1 has been identified as the (adenylyl) cyclase-associated protein. Cap1 was able to bind Cap1 itself and actin. Cap1 localized at the growing tip, and this localization was dependent on the Cap1 P2 region. In a two-hybrid screening using cap1 as bait, we isolated csh3, which encodes a pro...

journal_title：FEMS yeast research

authors： Yamamoto T,Kobayashi-Ooka Y,Zhou GL,Kawamukai M

更新日期：2015-12-01 00:00:00

abstract：:A salt-sensitive mutant of Kluyveromyces lactis was isolated that was unable to grow in high-salt media. This mutant was also respiratory-deficient and temperature-sensitive for growth. The mutation mapped in a single nuclear gene that is the ortholog of BCS1 of Saccharomyces cerevisiae. The BCS1 product is a mitochon...

journal_title：FEMS yeast research

authors： Goffrini P

更新日期：2007-03-01 00:00:00

abstract：:Genetic relationships of 24 phenotypically different strains isolated from sorghum beer in West Africa and the type cultures of the Saccharomyces sensu stricto species were investigated by universally primed polymerase chain reaction (PCR) analysis, microsatellite fingerprinting and PCR-restriction fragment length pol...

journal_title：FEMS yeast research

authors： Naumova ES,Korshunova IV,Jespersen L,Naumov GI

更新日期：2003-04-01 00:00:00

abstract：:The rDNA sequencing data obtained during the last 5 years in several laboratories clearly demonstrate that within the heterogeneous genus Kluyveromyces there is a group of highly related species, which we refer to as the genus Zygofabospora Kudriavzev 1960 emend. G. Naumov 2002. This genus includes four hybridizing sp...

journal_title：FEMS yeast research

authors： Naumov GI,Naumova ES

更新日期：2002-03-01 00:00:00

abstract：:The vast majority of wine fermentations are performed principally by Saccharomyces cerevisiae. However, there are a growing number of instances in which other species of Saccharomyces play a predominant role. Interestingly, the presence of these other yeast species generally occurs via the formation of interspecific h...

journal_title：FEMS yeast research

authors： Borneman AR,Desany BA,Riches D,Affourtit JP,Forgan AH,Pretorius IS,Egholm M,Chambers PJ

更新日期：2012-02-01 00:00:00

abstract：:The progenitor of the Dekkera/Brettanomyces clade separated from the Saccharomyces/Kluyveromyces clade over 200 million years ago. However, within both clades, several lineages developed similar physiological traits. Both Saccharomyces cerevisiae and Dekkera bruxellensis are facultative anaerobes; in the presence of e...

journal_title：FEMS yeast research

authors： Procházka E,Poláková S,Piskur J,Sulo P

更新日期：2010-08-01 00:00:00

abstract：:Malassezia pachydermatis isolates (n=185) from skin sites from dogs (n=30) were characterized genetically and biochemically following in vitro culture. Two regions in the chitin synthase-2 gene (chs-2) and the first internal transcribed spacer (ITS-1) of nuclear ribosomal DNA were sequenced, and the phospholipase acti...

journal_title：FEMS yeast research

authors： Cafarchia C,Gasser RB,Latrofa MS,Parisi A,Campbell BE,Otranto D

更新日期：2008-05-01 00:00:00

abstract：:Penicillin G amidase from Providencia rettgeri is a heterodimer of 92 kDa. We have previously expressed the Pr. rettgeri pac gene coding for this enzyme in Saccharomyces cerevisiae, and now we report the expression and characterization in the methylotrophic yeast Pichia pastoris. The recombinant catalytically active e...

journal_title：FEMS yeast research

authors： Sevo M,Degrassi G,Skoko N,Venturi V,Ljubijankić G

更新日期：2002-01-01 00:00:00

abstract：:An extended definition of the term metabolic engineering is given and its successful use in the construction of biorecognition elements of sensors is demonstrated. It is shown that genetic and chemical modifications of methylotrophic yeast cells provide directed changes in their physiological responses towards methano...

journal_title：FEMS yeast research

authors： Gonchar M,Maidan M,Korpan Y,Sibirny V,Kotylak Z,Sibirny A

更新日期：2002-08-01 00:00:00

abstract：:The GPD1 gene encoding the glycerol-3-phosphate dehydrogenase was overexpressed in an industrial lager brewing yeast (Saccharomyces cerevisiae ssp. carlsbergensis) to reduce the content of ethanol in beer. The amount of glycerol produced by the GPD1-overexpressing yeast in fermentation experiments simulating brewing c...

journal_title：FEMS yeast research

authors： Nevoigt E,Pilger R,Mast-Gerlach E,Schmidt U,Freihammer S,Eschenbrenner M,Garbe L,Stahl U

更新日期：2002-05-01 00:00:00

abstract：:The ultimate goal of therapeutic vaccines is to activate and exploit the patient's own immune system to vigorously and dynamically seek and eradicate established malignant or virally infected cells. Therapeutic vaccines also offer the potential for preventing disease recurrence. Saccharomyces cerevisiae-based vaccines...

journal_title：FEMS yeast research

authors： Ardiani A,Higgins JP,Hodge JW

更新日期：2010-12-01 00:00:00

abstract：:Evaluation of the functional impact of cancer-associated missense variants is more difficult than for protein-truncating mutations and consequently standard guidelines for the interpretation of sequence variants have been recently proposed. A number of algorithms and software products were developed to predict the imp...

journal_title：FEMS yeast research

authors： Mercatanti A,Lodovichi S,Cervelli T,Galli A

更新日期：2017-12-01 00:00:00