Elucidating cellular mechanisms of Saccharomyces cerevisiae tolerant to combined lignocellulosic-derived inhibitors using high-throughput phenotyping and multiomics analyses.


:A robust cell factory that can tolerate combined inhibitory lignocellulosic compounds is essential for the cost-effective lignocellulose-based production of second-generation bioethanol and other bulk chemicals. Following high-throughput phenotyping of a yeast genomic overexpression library, we identified a Saccharomyces cerevisiae mutant (denoted AFb.01) with improved growth and fermentation performance under combined toxicity of acetic acid and furfural. AFb.01 carries overexpression of TRX1, which encodes for thioredoxin, a cellular redox machinery. Through comparative proteomics and metabolomics, the resulting cell-wide changes in the mutant were elucidated and these primarily target on the maintenance of energy and redox homeostasis and the minimization of stress-induced cell damages. In particular, the upregulation of the stress-response proteins Hsp26p and Fmp16p conferred tolerance of AFb.01 against protein denaturation and DNA damage. Moreover, increased levels of protectant metabolites such as trehalose, fatty acids, GABA and putrescine provided additional defense mechanisms for the mutant against oxidative and redox stresses. Future studies will concentrate on targeted genetic engineering to validate these mechanisms as well as to support the creation of more robust yeast strains, applicable for industrial, cost-competitive biorefinery production.


FEMS Yeast Res


FEMS yeast research


Unrean P,Gätgens J,Klein B,Noack S,Champreda V




Has Abstract


2018-12-01 00:00:00












  • Cdc37p is involved in osmoadaptation and controls high osmolarity-induced cross-talk via the MAP kinase Kss1p.

    abstract::Cdc37p, the p50 homolog of Saccharomyces cerevisiae, is an Hsp90 cochaperone involved in the targeting of protein kinases to Hsp90. Here we report a role for Cdc37p in osmoadaptive signalling in this yeast. The osmosensitive phenotype that is displayed by the cdc37-34 mutant strain appears not to be the consequence of...

    journal_title:FEMS yeast research

    pub_type: 杂志文章


    authors: Yang XX,Hawle P,Bebelman JP,Meenhuis A,Siderius M,van der Vies SM

    更新日期:2007-09-01 00:00:00

  • Contemplating the murine test tube: lessons from natural killer cells and Cryptococcus neoformans.

    abstract::Murine experimentation has provided many useful tools, including the ability to knockout or over-express genes and to perform experiments that are limited by ethical considerations. Over the past century, mice have imparted valuable insights into the biology of many systems, including human immunity. However, although...

    journal_title:FEMS yeast research

    pub_type: 杂志文章,评审


    authors: Marr KJ,Jones GJ,Mody CH

    更新日期:2006-06-01 00:00:00

  • Methionine catabolism in Saccharomyces cerevisiae.

    abstract::The catabolism of methionine to methionol and methanethiol in Saccharomyces cerevisiae was studied using (13)C NMR spectroscopy, GC-MS, enzyme assays and a number of mutants. Methionine is first transaminated to alpha-keto-gamma-(methylthio)butyrate. Methionol is formed by a decarboxylation reaction, which yields meth...

    journal_title:FEMS yeast research

    pub_type: 杂志文章


    authors: Perpète P,Duthoit O,De Maeyer S,Imray L,Lawton AI,Stavropoulos KE,Gitonga VW,Hewlins MJ,Dickinson JR

    更新日期:2006-01-01 00:00:00

  • Physical interaction between Sit1 and Aft1 upregulates FOB uptake activity by inhibiting protein degradation of Sit1 in Saccharomyces cerevisiae.

    abstract::Previously, we reported that Aft1 regulates Sit1 by modulating the ubiquitination of Sit1 in Saccharomyces cerevisiae. Here, we report the function of the physical interaction between Sit1 and Aft1 in ferrioxamine B (FOB) uptake. The interaction between Sit1 and Aft1 induced protein localization of Sit1 to the plasma ...

    journal_title:FEMS yeast research

    pub_type: 杂志文章


    authors: Kang CM,Kang S,Park YS,Yun CW

    更新日期:2015-11-01 00:00:00

  • Microbial alcohol-conferred hemolysis is a late response to alcohol stress.

    abstract::We have reported previously that growth on alcohol vapors confers hemolytic properties on certain yeast species and strains ['microbial alcohol-conferred hemolysis' (MACH)]. In a recent study, we analyzed the genetic basis of MACH in Saccharomyces cerevisiae using the EUROSCARF mutant collection. The data suggested th...

    journal_title:FEMS yeast research

    pub_type: 杂志文章


    authors: Shuster A,Korem M,Jacob-Hirsch J,Amariglio N,Rechavi G,Rosenberg M

    更新日期:2011-06-01 00:00:00

  • Phosphorylation of Bni4 by MAP kinases contributes to septum assembly during yeast cytokinesis.

    abstract::Previous work has shown that the synthetic lethality of the slt2Δrim101Δ mutant results from a combination of factors, including improper functioning of the septum assembly machinery. Here, we identify new multicopy suppressors of this lethality including Kss1, Pcl1 and Sph1, none of which seems to be linked to the up...

    journal_title:FEMS yeast research

    pub_type: 杂志文章


    authors: Pérez J,Arcones I,Gómez A,Casquero V,Roncero C

    更新日期:2016-09-01 00:00:00

  • Zygosaccharomyces pseudobailii, another yeast interspecies hybrid that regained fertility by damaging one of its MAT loci.

    abstract::Interspecies hybridization is an important evolutionary mechanism in yeasts. The genus Zygosaccharomyces in particular contains numerous hybrid strains and/or species. Here, we investigated the genome of Zygosaccharomyces strain MT15, an isolate from Maotai-flavor Chinese liquor fermentation. We found that it is an in...

    journal_title:FEMS yeast research

    pub_type: 杂志文章


    authors: Braun-Galleani S,Ortiz-Merino RA,Wu Q,Xu Y,Wolfe KH

    更新日期:2018-11-01 00:00:00

  • Heat shock-inducible expression vectors for use in Schizosaccharomyces pombe.

    abstract::A new, heat shock-inducible expression system based on an endogenous hsp16+ promoter was developed for use in the fission yeast Schizosaccharomyces pombe. Analysis of GFP expression profiles indicated that a 1.2-kb segment of the hsp16+ promoter region was sufficient to drive expression of heterologous protein. The hs...

    journal_title:FEMS yeast research

    pub_type: 杂志文章


    authors: Fujita Y,Tohda H,Giga-Hama Y,Takegawa K

    更新日期:2006-09-01 00:00:00

  • Yarrowia lipolytica: a model yeast for citric acid production.

    abstract::Every year more than 2 million tons of citric acid (CA) are produced around the world for industrial uses. Although initially extracted from citrus, the low profitability of the process and the increasing demand soon stimulated the search for more efficient methods to produce CA. Currently, most world CA demand (99%) ...

    journal_title:FEMS yeast research

    pub_type: 杂志文章


    authors: Cavallo E,Charreau H,Cerrutti P,Foresti ML

    更新日期:2017-12-01 00:00:00

  • Improved raw starch amylase production by Saccharomyces cerevisiae using codon optimisation strategies.

    abstract::Amylases are used in a variety of industries that have a specific need for alternative enzymes capable of hydrolysing raw starch. Five α-amylase and five glucoamylase-encoding genes were expressed in the Saccharomyces cerevisiae Y294 laboratory strain to select for recombinant strains that best hydrolysed raw corn sta...

    journal_title:FEMS yeast research

    pub_type: 杂志文章


    authors: Cripwell RA,Rose SH,Viljoen-Bloom M,van Zyl WH

    更新日期:2019-03-01 00:00:00

  • Dextranase (alpha-1,6 glucan-6-glucanohydrolase) from Penicillium minioluteum expressed in Pichia pastoris: two host cells with minor differences in N-glycosylation.

    abstract::Differences in glycosylation between the natural alpha-1,6 glucan-6-glucanohydrolase from Penicillium minioluteum and the heterologous protein expressed in the yeast Pichia pastoris were analyzed. Glycosylation profiling was carried out using fluorophore-assisted carbohydrate electrophoresis and amine absorption high-...

    journal_title:FEMS yeast research

    pub_type: 杂志文章


    authors: Betancourt LH,García R,González J,Montesino R,Quintero O,Takao T,Shimonishi Y,Cremata JA

    更新日期:2001-07-01 00:00:00

  • The strange case of a biofilm-forming strain of Pichia fermentans, which controls Monilinia brown rot on apple but is pathogenic on peach fruit.

    abstract::A biofilm-forming strain of Pichia fermentans proved to be most effective in controlling brown rot on apple fruit when coinoculated into artificial wounds with a phytopathogenic isolate of Monilinia fructicola. Culture filtrates and autoclaved cells had no significant influence on the disease. When sprayed onto the ap...

    journal_title:FEMS yeast research

    pub_type: 杂志文章


    authors: Giobbe S,Marceddu S,Scherm B,Zara G,Mazzarello VL,Budroni M,Migheli Q

    更新日期:2007-12-01 00:00:00

  • Cell size and morphological properties of yeast Saccharomyces cerevisiae in relation to growth temperature.

    abstract::Cell volume is an important parameter for modelling cellular processes. Temperature-induced variability of cellular size, volume, intracellular granularity, a fraction of budding cells of yeast Saccharomyces cerevisiae CEN.PK 113-7D (in anaerobic glucose unlimited batch cultures) were measured by flow cytometry and ma...

    journal_title:FEMS yeast research

    pub_type: 杂志文章


    authors: Zakhartsev M,Reuss M

    更新日期:2018-09-01 00:00:00

  • Tipping the balance both ways: drug resistance and virulence in Candida glabrata.

    abstract::Among existing fungal pathogens, Candida glabrata is outstanding in its capacity to rapidly develop resistance to currently used antifungal agents. Resistance to the class of azoles, which are still widely used agents, varies in proportion (from 5 to 20%) depending on geographical area. Moreover, resistance to the cla...

    journal_title:FEMS yeast research

    pub_type: 杂志文章,评审


    authors: Vale-Silva LA,Sanglard D

    更新日期:2015-06-01 00:00:00

  • The interplay between sulphur and selenium metabolism influences the intracellular redox balance in Saccharomyces cerevisiae.

    abstract::Selenium (Se) is an essential element for most eukaryotic organisms, including humans. The balance between Se toxicity and its beneficial effects is very delicate. It has been demonstrated that a diet enriched with Se has cancer prevention potential in humans. The most popular commercial Se supplementation is selenize...

    journal_title:FEMS yeast research

    pub_type: 杂志文章


    authors: Mapelli V,Hillestrøm PR,Patil K,Larsen EH,Olsson L

    更新日期:2012-02-01 00:00:00

  • Life without putrescine: disruption of the gene-encoding polyamine oxidase in Ustilago maydis odc mutants.

    abstract::In previous communications the essential role of spermidine in Ustilago maydis was demonstrated by means of the disruption of the genes encoding ornithine decarboxylase (ODC) and spermidine synthase (SPE). However, the assignation of specific roles to each polyamine in different cellular functions was not possible bec...

    journal_title:FEMS yeast research

    pub_type: 杂志文章


    authors: Valdés-Santiago L,Guzmán-de-Peña D,Ruiz-Herrera J

    更新日期:2010-11-01 00:00:00

  • Evolution of regulatory networks in Candida glabrata: learning to live with the human host.

    abstract::The opportunistic human fungal pathogen Candida glabrata is second only to C. albicans as the cause of Candida infections and yet is more closely related to Saccharomyces cerevisiae. Recent advances in functional genomics technologies and computational approaches to decipher regulatory networks, and the comparison of ...

    journal_title:FEMS yeast research

    pub_type: 杂志文章,评审


    authors: Roy S,Thompson D

    更新日期:2015-12-01 00:00:00

  • Ascospores of large-spored Metschnikowia species are genuine meiotic products of these yeasts.

    abstract::The asci of Metschnikowia species normally contain two ascospores (never more), raising the question of whether these spores are true meiotic products. We investigated this problem by crossing genetically-marked strains of the haploid, heterothallic taxa Metschnikowia hawaiiensis, Metschnikowia continentalis var. cont...

    journal_title:FEMS yeast research

    pub_type: 杂志文章


    authors: Marinoni G,Piskur J,Lachance MA

    更新日期:2003-03-01 00:00:00

  • Molecular genetic identification of Saccharomyces sensu stricto strains from African sorghum beer.

    abstract::Genetic relationships of 24 phenotypically different strains isolated from sorghum beer in West Africa and the type cultures of the Saccharomyces sensu stricto species were investigated by universally primed polymerase chain reaction (PCR) analysis, microsatellite fingerprinting and PCR-restriction fragment length pol...

    journal_title:FEMS yeast research

    pub_type: 杂志文章


    authors: Naumova ES,Korshunova IV,Jespersen L,Naumov GI

    更新日期:2003-04-01 00:00:00

  • Ubiquinone biosynthesis in Saccharomyces cerevisiae: the molecular organization of O-methylase Coq3p depends on Abc1p/Coq8p.

    abstract::Coenzyme Q is a redox-active lipid that functions as an electron carrier in the mitochondrial respiratory chain. Q-biosynthesis in Saccharomyces cerevisiae requires at least nine proteins (Coq1p-Coq9p). The molecular function of Coq8p is still unknown; however, lack of Q and the concomitant accumulation of the interme...

    journal_title:FEMS yeast research

    pub_type: 杂志文章


    authors: Tauche A,Krause-Buchholz U,Rödel G

    更新日期:2008-12-01 00:00:00

  • Discrete roles of trehalose and Hsp104 in inhibition of protein aggregation in yeast cells.

    abstract::Heat shock response (HSR) is an important element of cellular homeostasis. In yeast, HSR comprises of the heat shock proteins (Hsps) and the osmolytes trehalose and glycerol. The respective roles of trehalose and Hsp104 in regulating protein aggregation remain ambiguous. We report that trehalose and Hsp104 are importa...

    journal_title:FEMS yeast research

    pub_type: 杂志文章


    authors: Sethi R,Iyer SS,Das E,Roy I

    更新日期:2018-09-01 00:00:00

  • The oxygen level determines the fermentation pattern in Kluyveromyces lactis.

    abstract::Yeasts belonging to the lineage that underwent whole-genome duplication (WGD) possess a good fermentative potential and can proliferate in the absence of oxygen. In this study, we analyzed the pre-WGD yeast Kluyveromyces lactis and its ability to grow under oxygen-limited conditions. Under these conditions, K. lactis ...

    journal_title:FEMS yeast research

    pub_type: 杂志文章


    authors: Merico A,Galafassi S,Piskur J,Compagno C

    更新日期:2009-08-01 00:00:00

  • Torulaspora delbrueckii produces high levels of C5 and C6 polyols during wine fermentations.

    abstract::Non-Saccharomyces yeasts impact wine fermentations and can diversify the flavor profiles of wines. However, little information is available on the metabolic networks of most of these species. Here we show that unlike the main wine yeast Saccharomyces cerevisiae, Torulaspora delbrueckii and to a lesser extent Lachancea...

    journal_title:FEMS yeast research

    pub_type: 杂志文章


    authors: Mbuyane LL,de Kock M,Bauer FF,Divol B

    更新日期:2018-11-01 00:00:00

  • New species and new combinations in the yeast genera Kregervanrija gen. nov., Saturnispora and Candida.

    abstract::Analysis of nucleotide sequences from the domains 1 and 2 of the large-subunit rDNA demonstrated species of the Pichia fluxuum clade to be phylogenetically isolated, and domains 1 and 2 and internal transcribed spacer rDNA sequence analyses of strains phenotypically identified as P. fluxuum resulted in the discovery o...

    journal_title:FEMS yeast research

    pub_type: 杂志文章


    authors: Kurtzman CP

    更新日期:2006-03-01 00:00:00

  • Incidence of symbiotic dsRNA 'killer' viruses in wild and domesticated yeast.

    abstract::Viruses are found in almost all organisms and physical habitats. One interesting example is the yeast viral 'killer system'. The virus provides the host with a toxin directed against strains that do not carry it, while the yeast cell enables its propagation. Although yeast viruses are believed to be common, they have ...

    journal_title:FEMS yeast research

    pub_type: 杂志文章


    authors: Pieczynska MD,de Visser JA,Korona R

    更新日期:2013-12-01 00:00:00

  • Preliminary characterisation of DML1, an essential Saccharomyces cerevisiae gene related to misato of Drosophila melanogaster.

    abstract::A genetic and cell-biological analysis is provided for Saccharomyces cerevisiae DML1 (YMR211w) encoding a Drosophila melanogaster Misato-like protein. Misato and Dml1p are descendants of an ancestral tubulin-like protein, and exhibit regions with similarity to members of a GTPase family that include eukaryotic tubulin...

    journal_title:FEMS yeast research

    pub_type: 杂志文章


    authors: Gurvitz A,Hartig A,Ruis H,Hamilton B,de Couet HG

    更新日期:2002-05-01 00:00:00

  • Humoral immune responses to Candida albicans complement receptor 3-related protein in the atopic subjects with vulvovaginal candidiasis. Novel sensitive marker for Candida infection.

    abstract::In vitro evaluation of specific anti-Candida albicans sera antibodies based on synthetically prepared complement receptor 3-related protein (CR3-RP) mimicking the structure of native complement receptor 3 in a cohort of 72 patients with atopy and recurrent Candida vulvovaginitis (RVC) revealed effective humoral respon...

    journal_title:FEMS yeast research

    pub_type: 杂志文章


    authors: Paulovičová E,Bujdáková H,Chupáčová J,Paulovičová L,Kertys P,Hrubiško M

    更新日期:2015-03-01 00:00:00

  • Expression of the alpha3/beta1 isoform of human Na,K-ATPase in the methylotrophic yeast Pichia pastoris.

    abstract::Na,K-ATPase is a crucial enzyme for ion homeostasis in human tissues. Different isozymes are produced by assembly of four alpha- and three beta-subunits. The expression of the alpha3/beta1 isozyme is confined to brain and heart. Its heterologous production has so far never been attempted in a lower eukaryote. In this ...

    journal_title:FEMS yeast research

    pub_type: 杂志文章


    authors: Reina C,Padoani G,Carotti C,Merico A,Tripodi G,Ferrari P,Popolo L

    更新日期:2007-06-01 00:00:00

  • Vaccines based on whole recombinant Saccharomyces cerevisiae cells.

    abstract::The ultimate goal of therapeutic vaccines is to activate and exploit the patient's own immune system to vigorously and dynamically seek and eradicate established malignant or virally infected cells. Therapeutic vaccines also offer the potential for preventing disease recurrence. Saccharomyces cerevisiae-based vaccines...

    journal_title:FEMS yeast research

    pub_type: 杂志文章,评审


    authors: Ardiani A,Higgins JP,Hodge JW

    更新日期:2010-12-01 00:00:00

  • Pkc1 and actin polymerisation activities play a role in ribosomal gene repression associated with secretion impairment caused by oxidative stress.

    abstract::In Saccharomyces cerevisiae, the cell integrity pathway plays a role in the oxidative stress response. In this study, we show that the Pkc1 protein mediates oxidative signalling by helping to downregulate ribosomal gene expression when cells are exposed to hydrogen peroxide. An active actin cytoskeleton is required fo...

    journal_title:FEMS yeast research

    pub_type: 杂志文章


    authors: Mitjana FV,Petkova MI,Pujol-Carrion N,de la Torre-Ruiz MA

    更新日期:2011-12-01 00:00:00