Abstract:
:In the methylotrophic yeast Hansenula polymorpha non-selective autophagy, induced by nitrogen starvation, results in the turnover of cytoplasmic components, including peroxisomes. We show that the uptake of these components occurs by invagination of the vacuolar membrane without their prior sequestration and thus differs from the mechanism described for bakers yeast. A selective mode of autophagy in H. polymorpha, namely glucose-induced peroxisome degradation, involves sequestration of individual peroxisomes tagged for degradation by membrane layers that subsequently fuse with the vacuole where the organelle is digested. H. polymorpha pdd mutants are blocked in selective peroxisome degradation. We observed that pdd1-201 is also impaired in non-selective autophagy, whereas this process still normally functions in pdd2-4. These findings suggest that mechanistically distinct processes as selective and non-selective autophagy involve common but also unique genes.
journal_name
FEMS Yeast Resjournal_title
FEMS yeast researchauthors
Bellu AR,Kram AM,Kiel JA,Veenhuis M,van der Klei IJdoi
10.1111/j.1567-1364.2001.tb00010.xkeywords:
subject
Has Abstractpub_date
2001-04-01 00:00:00pages
23-31issue
1eissn
1567-1356issn
1567-1364pii
S1567135600000040journal_volume
1pub_type
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