Abstract:
:In the pathogenic fungus Candida glabrata, the YPS1 gene, which encodes a glycosylphosphatidylinositol-linked aspartyl protease, is required for cell wall integrity and virulence. Although the expression of YPS1 has been studied in Saccharomyces cerevisiae, the transcriptional regulation of this gene in C. glabrata is not well understood. Here, we report that C. glabrata Yps1 is required for cell growth at elevated temperatures, and that the heat-induced expression of YPS1 is regulated predominantly by the calcineurin-Crz1 pathway and partially by the Slt2 MAPK pathway. Although a total of 11 YPS genes are present in the C. glabrata genome, the loss of transcriptional induction in a calcineurin mutant was observed only for YPS1. The results of a YPS1 promoter-lacZ reporter assay using a series of constructs with mutated promoter elements indicated that the transcription factor Crz1 binds to multiple sites in the promoter region of YPS1. To date, as none of the putative Crz1 targets in C. glabrata have been characterized using a Δcrz1 mutant, monitoring the expression of YPS1 represents an effective method for measuring the activity of the calcineurin-Crz1 signaling pathway in this fungus.
journal_name
FEMS Yeast Resjournal_title
FEMS yeast researchauthors
Miyazaki T,Izumikawa K,Yamauchi S,Inamine T,Nagayoshi Y,Saijo T,Seki M,Kakeya H,Yamamoto Y,Yanagihara K,Miyazaki Y,Yasuoka A,Kohno Sdoi
10.1111/j.1567-1364.2011.00734.xsubject
Has Abstractpub_date
2011-08-01 00:00:00pages
449-56issue
5eissn
1567-1356issn
1567-1364journal_volume
11pub_type
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