Molecular genetic identification of Saccharomyces sensu stricto strains from African sorghum beer.

Abstract:

:Genetic relationships of 24 phenotypically different strains isolated from sorghum beer in West Africa and the type cultures of the Saccharomyces sensu stricto species were investigated by universally primed polymerase chain reaction (PCR) analysis, microsatellite fingerprinting and PCR-restriction fragment length polymorphism (RFLP) of the ribosomal internal transcribed spacers. The results demonstrate that internal transcribed spacer (ITS) PCR-RFLP analysis with the endonucleases HaeIII, HpaII, ScrFI and TaqI is useful for discriminating S. cerevisiae, S. kudriavzevii, S. mikatae from one another and from the S. bayanus/S. pastorianus and S. cariocanus/S. paradoxus pairs. The sorghum beer strains exhibited the same restriction patterns as the type culture of S. cerevisiae CBS 1171. PCR profiles generated with the microsatellite primer (GTG)(5) and the universal primer N21 were almost identical for all isolates and strain CBS 1171. Despite phenotypic peculiarities, the strains involved in sorghum beer production in Ghana and Burkina Faso belong to S. cerevisiae. However, based on sequencing of the rDNA ITS1 region and Southern hybridisation analysis, these strains represent a divergent population of S. cerevisiae.

journal_name

FEMS Yeast Res

journal_title

FEMS yeast research

authors

Naumova ES,Korshunova IV,Jespersen L,Naumov GI

doi

10.1016/S1567-1356(02)00191-5

keywords:

subject

Has Abstract

pub_date

2003-04-01 00:00:00

pages

177-84

issue

2

eissn

1567-1356

issn

1567-1364

pii

S1567135602001915

journal_volume

3

pub_type

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