Methionine catabolism in Saccharomyces cerevisiae.

abstract：:Xylose fermentation is a rare trait that is immensely important to the cellulosic biofuel industry, and Candida tenuis is one of the few yeasts that has been reported with this trait. Here we report the isolation of two strains representing a candidate sister species to C. tenuis. Integrated analysis of genome sequenc...

journal_title：FEMS yeast research

authors： Haase MAB,Kominek J,Langdon QK,Kurtzman CP,Hittinger CT

更新日期：2017-05-01 00:00:00

abstract：:In yeast, rRNA genes can be detected with the FISH technique using rRNA gene probes. This technique yields reliable, reproducible and precise results, but is time-consuming. Here, the primed in situ DNA synthesis (PRINS) procedure has been optimized for rapid detection of yeast rRNA genes. PRINS, which is as sensitive...

journal_title：FEMS yeast research

authors： Wnuk M,Lewinska A,Bugno M,Bartosz G,Slota E

更新日期：2009-06-01 00:00:00

abstract：:Ume6p is a nonessential transcription factor that represses meiotic gene expression during vegetative growth in budding yeast. To relieve this repression, Ume6p is destroyed as cells enter meiosis and is not resynthesized until spore wall assembly. The present study reveals that spores derived from a ume6 null homozyg...

journal_title：FEMS yeast research

authors： Strich R,Khakhina S,Mallory MJ

更新日期：2011-02-01 00:00:00

abstract：:Amylases are used in a variety of industries that have a specific need for alternative enzymes capable of hydrolysing raw starch. Five α-amylase and five glucoamylase-encoding genes were expressed in the Saccharomyces cerevisiae Y294 laboratory strain to select for recombinant strains that best hydrolysed raw corn sta...

journal_title：FEMS yeast research

authors： Cripwell RA,Rose SH,Viljoen-Bloom M,van Zyl WH

更新日期：2019-03-01 00:00:00

abstract：:Saccharomyces cerevisiae is an excellent ethanol producer, but is rather sensitive to high concentration of ethanol. Here, influences of ethanol on cellular membrane integrity and carbon metabolism of S. cerevisiae were investigated to rationalize mechanism involved in ethanol toxicity. Addition of 5% (v/v) ethanol di...

journal_title：FEMS yeast research

authors： Yang KM,Lee NR,Woo JM,Choi W,Zimmermann M,Blank LM,Park JB

更新日期：2012-09-01 00:00:00

abstract：:Messenger RNAs retained in the nucleus of Saccharomyces cerevisiae are subjected to a degradation system designated DRN (Degradation of mRNA in the Nucleus) that is dependent on the nuclear mRNA cap-binding protein, Cbc1p, as well as nuclear exosome component Rrp6p, a 3' to 5' exoribonuclease. DRN has been shown to ac...

journal_title：FEMS yeast research

authors： Das S,Saha U,Das B

更新日期：2014-09-01 00:00:00

abstract：:Yarrowia lipolytica is a dimorphic fungus whose morphology is controlled by several factors such as pH and different compounds. To determine if the STE11-mitogen-activated protein kinase (MAPK) pathway plays a role in dimorphism of Y. lipolytica, we isolated the gene encoding a Mapkkk. The isolated gene (STE11) has an...

journal_title：FEMS yeast research

authors： Cervantes-Chávez JA,Ruiz-Herrera J

更新日期：2006-08-01 00:00:00

abstract：:A robust cell factory that can tolerate combined inhibitory lignocellulosic compounds is essential for the cost-effective lignocellulose-based production of second-generation bioethanol and other bulk chemicals. Following high-throughput phenotyping of a yeast genomic overexpression library, we identified a Saccharomy...

journal_title：FEMS yeast research

authors： Unrean P,Gätgens J,Klein B,Noack S,Champreda V

更新日期：2018-12-01 00:00:00

abstract：:The genus Schizosaccharomyces is presently comprised of three species, namely Schizosaccharomyces pombe, Schizosaccharomyces octosporus and Schizosaccharomyces japonicus. Here, we describe a hitherto unknown species, Schizosaccharomyces cryophilus, named for its preference for growth at lower temperatures than the oth...

journal_title：FEMS yeast research

authors： Helston RM,Box JA,Tang W,Baumann P

更新日期：2010-09-01 00:00:00

abstract：:Maf1 is a repressor of RNA polymerase (Pol) III transcription for tRNA. Nutrient deprivation and environmental stress repress Pol III transcription through Maf1 in Saccharomyces cerevisiae. The sole Candida albicans homolog CaMaf1 is a protein of 380 amino acids with conserved domains and motifs of the eukaryotic Maf1...

journal_title：FEMS yeast research

authors： Asghar F,Yan H,Jiang L

更新日期：2018-09-01 00:00:00

abstract：:Kluyveromyces marxianus is a safe yeast used in the food and biotechnology sectors. One of the important traits that sets it apart from the familiar yeasts, Saccharomyces cerevisiae, is its capacity to grow using lactose as a carbon source. Like in its close relative, Kluyveromyces lactis, this requires lactose transp...

journal_title：FEMS yeast research

authors： Varela JA,Montini N,Scully D,Van der Ploeg R,Oreb M,Boles E,Hirota J,Akada R,Hoshida H,Morrissey JP

更新日期：2017-05-01 00:00:00

abstract：:In Kluyveromyces lactis, galactose transport has been thought to be mediated by the lactose permease encoded by LAC12. In fact, a lac12 mutant unable to grow on lactose did not grow on galactose either and showed low and uninducible galactose uptake activity. The existence of other galactose transport systems, at low ...

journal_title：FEMS yeast research

authors： Baruffini E,Goffrini P,Donnini C,Lodi T

更新日期：2006-12-01 00:00:00

abstract：:The yeast Pichia anomala J121 prevents mold spoilage and enhances preservation of moist grain in malfunctioning storage systems. Development of P. anomala J121 as a biocontrol agent requires in-depth knowledge about its physiology. P. anomala J121 grew under strictly anaerobic conditions, at temperatures between 3 deg...

journal_title：FEMS yeast research

authors： Fredlund E,Druvefors U,Boysen ME,Lingsten KJ,Schnürer J

更新日期：2002-08-01 00:00:00

abstract：:The mechanism involved in the cellular phosphate response of Saccharomyces cerevisiae forms part of the PHO pathway, which upon expression allows a co-ordinated cellular response and adaptation to changes in availability of external phosphate. Although genetic studies and analyses of the S. cerevisiae genome have prod...

journal_title：FEMS yeast research

authors： Mouillon JM,Persson BL

更新日期：2006-03-01 00:00:00

abstract：:The aim of this retrospective study was to analyze the relationship between occurrence of the serotypes of the Cryptococcus neoformans species complex in tree samples and the climatic conditions registered during samplings in four cities of Colombia, between 1992 and 2004, by means of a logistic regression model and l...

journal_title：FEMS yeast research

authors： Granados DP,Castañeda E

更新日期：2006-06-01 00:00:00

abstract：:Cdc37p, the p50 homolog of Saccharomyces cerevisiae, is an Hsp90 cochaperone involved in the targeting of protein kinases to Hsp90. Here we report a role for Cdc37p in osmoadaptive signalling in this yeast. The osmosensitive phenotype that is displayed by the cdc37-34 mutant strain appears not to be the consequence of...

journal_title：FEMS yeast research

authors： Yang XX,Hawle P,Bebelman JP,Meenhuis A,Siderius M,van der Vies SM

更新日期：2007-09-01 00:00:00

abstract：:Endosomal sorting complexes required for transport (ESCRTs) are involved in the formation of multivesicular bodies and sorting of targeted proteins to the yeast vacuole. The deletion of seven genes encoding components of the ESCRT machinery render Saccharomyces cerevisiae cells sensitive to high extracellular CaCl2 co...

journal_title：FEMS yeast research

authors： Papouskova K,Jiang L,Sychrova H

更新日期：2015-03-01 00:00:00

abstract：:Heat shock response (HSR) is an important element of cellular homeostasis. In yeast, HSR comprises of the heat shock proteins (Hsps) and the osmolytes trehalose and glycerol. The respective roles of trehalose and Hsp104 in regulating protein aggregation remain ambiguous. We report that trehalose and Hsp104 are importa...

journal_title：FEMS yeast research

authors： Sethi R,Iyer SS,Das E,Roy I

更新日期：2018-09-01 00:00:00

abstract：:The roles of intracellular GTPase Arl1 and organellar cation/H(+) antiporters (Kha1 and Nhx1) in Saccharomyces cerevisiae tolerance to various stress factors were investigated and interesting new phenotypes of strains devoid of these proteins were found. The role of Arl1 GTPase in their tolerance to various cations is...

journal_title：FEMS yeast research

authors： Marešová L,Sychrová H

更新日期：2010-11-01 00:00:00

abstract：:In previous communications the essential role of spermidine in Ustilago maydis was demonstrated by means of the disruption of the genes encoding ornithine decarboxylase (ODC) and spermidine synthase (SPE). However, the assignation of specific roles to each polyamine in different cellular functions was not possible bec...

journal_title：FEMS yeast research

authors： Valdés-Santiago L,Guzmán-de-Peña D,Ruiz-Herrera J

更新日期：2010-11-01 00:00:00

abstract：:Eight yeast strains that asexually reproduce by cell fission were isolated from bee bread of different solitary bees in Germany. DNA sequence analysis revealed that the strains shared the same sequence in the D1/D2 domain of the nuclear large subunit (LSU) rRNA gene with a strain that was previously isolated from a fi...

journal_title：FEMS yeast research

authors： Brysch-Herzberg M,Tobias A,Seidel M,Wittmann R,Wohlmann E,Fischer R,Dlauchy D,Peter G

更新日期：2019-06-01 00:00:00

abstract：:Genes of the post-squalene ergosterol biosynthetic pathway in Saccharomyces cerevisiae have been overexpressed in a systematic approach with the aim to construct yeast strains that produce high amounts of sterols from a squalene-accumulating strain. This strain had previously been deregulated by overexpressing a trunc...

journal_title：FEMS yeast research

authors： Veen M,Stahl U,Lang C

更新日期：2003-10-01 00:00:00

abstract：:We show that Arf3p, a member of the ADP ribosylation family, is involved in the organization of actin cables and cortical patches in Saccharomyces cerevisiae. Profilin-deficient cells (pfy1Delta) have severe growth defects and lack actin cables. Overexpression of ARF3 restores actin cables and corrects growth defects ...

journal_title：FEMS yeast research

authors： Lambert AA,Perron MP,Lavoie E,Pallotta D

更新日期：2007-09-01 00:00:00

abstract：:A novel method dissecting the regulation of a cellular function into direct metabolic regulation and hierarchical (e.g., gene-expression) regulation is applied to yeast starved for nitrogen or carbon. Upon nitrogen starvation glucose influx is down-regulated hierarchically. Upon carbon starvation it is down-regulated ...

journal_title：FEMS yeast research

authors： Rossell S,van der Weijden CC,Kruckeberg AL,Bakker BM,Westerhoff HV

更新日期：2005-04-01 00:00:00

abstract：:Wild-type strains of the thermotolerant methylotrophic yeast Hansenula polymorpha are able to ferment glucose, cellobiose and xylose to ethanol. H. polymorpha most actively fermented sugars to ethanol at 37 degrees C, whereas the well-known xylose-fermenting yeast Pichia stipitis could not effectively ferment carbon s...

journal_title：FEMS yeast research

authors： Ryabova OB,Chmil OM,Sibirny AA

更新日期：2003-11-01 00:00:00

abstract：:To investigate the role of the prevacuolar secretion pathway in the trafficking of vacuolar proteins in Candida albicans, the C. albicans homolog of the Saccharomyces cerevisiae vacuolar protein sorting gene VPS4 was cloned and analyzed. Candida albicans VPS4 encodes a deduced AAA-type ATPase that is 75.6% similar to ...

journal_title：FEMS yeast research

authors： Lee SA,Jones J,Khalique Z,Kot J,Alba M,Bernardo S,Seghal A,Wong B

更新日期：2007-09-01 00:00:00

abstract：:A biofilm-forming strain of Pichia fermentans proved to be most effective in controlling brown rot on apple fruit when coinoculated into artificial wounds with a phytopathogenic isolate of Monilinia fructicola. Culture filtrates and autoclaved cells had no significant influence on the disease. When sprayed onto the ap...

journal_title：FEMS yeast research

authors： Giobbe S,Marceddu S,Scherm B,Zara G,Mazzarello VL,Budroni M,Migheli Q

更新日期：2007-12-01 00:00:00

abstract：:Candida lipolytica and Candida rugosa were isolated from blood samples from a patient with chronic myeloid leukemia (31 years old) and a patient with sickle cell disease (1-year-old), respectively. Isolates were grown for 48 h at 37 degrees C in either Sabouraud or tryptone soy broth (TSB). Peptidases were tested for ...

journal_title：FEMS yeast research

authors： De Melo AC,Dornelas-Ribeiro M,De Souza EP,Macrae A,Fracalanzza SE,Vermelho AB

更新日期：2007-09-01 00:00:00

abstract：:Screening for genes affecting super-secreting phenotype of the over-secreting mutant of Kluyveromyces lactis resulted in isolation of the gene named KlMNN10, sharing high homology with Saccharomyces cerevisiae MNN10. The disruption of the KlMNN10 in Kluyveromyces lactis, as well as of MNN10 and MNN11 in Saccharomyces ...

journal_title：FEMS yeast research

authors： Bartkeviciūte D,Sasnauskas K

更新日期：2004-09-01 00:00:00

abstract：:Nine yeast strains were isolated from spontaneous fermentations in the Alsace area of France, during the 1997, 1998 and 1999 grape harvests. Strains were characterized by pulsed-field gel electrophoresis, PCR-restriction fragment length polymorphism (RFLP) of the MET2 gene, delta-PCR, and microsatellite patterns. Kary...

journal_title：FEMS yeast research

authors： Le Jeune C,Lollier M,Demuyter C,Erny C,Legras JL,Aigle M,Masneuf-Pomarède I

更新日期：2007-06-01 00:00:00