Abstract:
:Current paradigms assume that gas bubbles cannot be formed within yeasts although these workhorses of the baking and brewing industries vigorously produce and release CO(2) gas. We show that yeasts produce gas bubbles that fill a significant part of the cell. The missing link between intracellular CO(2) production by glycolysis and eventual CO(2) release from cells has therefore been resolved. Yeasts may serve as model to study CO(2) behavior under pressurized conditions that may impact on fermentation biotechnology.
journal_name
FEMS Yeast Resjournal_title
FEMS yeast researchauthors
Swart CW,Dithebe K,Pohl CH,Swart HC,Coetsee E,van Wyk PW,Swarts JC,Lodolo EJ,Kock JLdoi
10.1111/j.1567-1364.12004.xsubject
Has Abstractpub_date
2012-11-01 00:00:00pages
867-9issue
7eissn
1567-1356issn
1567-1364journal_volume
12pub_type
杂志文章abstract::High-level production of bioethanol (140 g L(-1) in 45 h) in aerated fed-batch cultures of Saccharomyces cerevisiae was shown to be linked to the length of a production phase uncoupled to the growth. The induction of this phase was characterized by metabolic and morphologic changes reminiscent of those occurring in th...
journal_title:FEMS yeast research
pub_type: 杂志文章
doi:10.1111/j.1567-1364.2009.00553.x
更新日期:2009-12-01 00:00:00
abstract::Candida albicans maintains genetic diversity by random chromosome alterations, and this diversity allows utilization of various nutrients. Although the alterations seem to occur spontaneously, their frequencies clearly depend on environmental factors. In addition, this microorganism survives in adverse environments, w...
journal_title:FEMS yeast research
pub_type: 杂志文章,评审
doi:10.1111/j.1567-1364.2006.00150.x
更新日期:2007-01-01 00:00:00
abstract::We have cloned the Hansenula polymorpha SWI1 and SNF2 genes by functional complementation of mutants that are defective in methanol utilisation. These genes encode proteins similar to Saccharomyces cerevisiae Swi1p and Snf2p, which are subunits of the SWI/SNF complex. This complex belongs to the family of nucleosome-r...
journal_title:FEMS yeast research
pub_type: 杂志文章
doi:10.1016/j.femsyr.2004.01.009
更新日期:2004-05-01 00:00:00
abstract::The most diffused formulation of starter for winemaking is active dry yeast (ADY). ADYs production process is essentially characterized by air-drying stress, a combination of several stresses, including thermal, hyperosmotic and oxidative and cell capacity to counteract such multiple stresses will determine its surviv...
journal_title:FEMS yeast research
pub_type: 杂志文章
doi:10.1093/femsyr/fow015
更新日期:2016-05-01 00:00:00
abstract::Differences in glycosylation between the natural alpha-1,6 glucan-6-glucanohydrolase from Penicillium minioluteum and the heterologous protein expressed in the yeast Pichia pastoris were analyzed. Glycosylation profiling was carried out using fluorophore-assisted carbohydrate electrophoresis and amine absorption high-...
journal_title:FEMS yeast research
pub_type: 杂志文章
doi:10.1111/j.1567-1364.2001.tb00026.x
更新日期:2001-07-01 00:00:00
abstract::The transcriptional response of laboratory strains of Saccharomyces cerevisiae to salt or sorbitol stress has been well studied. These studies have yielded valuable data on how the yeast adapts to these stress conditions. However, S. cerevisiae is a saccharophilic fungus and in its natural environment this yeast encou...
journal_title:FEMS yeast research
pub_type: 杂志文章
doi:10.1016/S1567-1356(02)00203-9
更新日期:2003-06-01 00:00:00
abstract::Three ascomycetous yeast strains were isolated from decaying green wine grapes, collected from Hyderabad city in India. Two strains, YS9 and YS21, were identified as Kodamaea ohmeri and Candida fermentati, respectively. The third strain, YS12(T), differs from Candida parapsilosis, Candida orthopsilosis and Candida met...
journal_title:FEMS yeast research
pub_type: 杂志文章
doi:10.1111/j.1567-1364.2006.00206.x
更新日期:2007-05-01 00:00:00
abstract::The ascomycetous yeasts traditionally referred to as the Saccharomyces sensu stricto complex are a group of closely related species that are isolated by a postzygotic barrier. They can easily hybridize; and their allodiploid hybrids propagate by mitotic divisions as efficiently as the parental strains, but can barely ...
journal_title:FEMS yeast research
pub_type: 杂志文章,评审
doi:10.1111/j.1567-1364.2008.00369.x
更新日期:2008-11-01 00:00:00
abstract::The genus Schizosaccharomyces is presently comprised of three species, namely Schizosaccharomyces pombe, Schizosaccharomyces octosporus and Schizosaccharomyces japonicus. Here, we describe a hitherto unknown species, Schizosaccharomyces cryophilus, named for its preference for growth at lower temperatures than the oth...
journal_title:FEMS yeast research
pub_type: 杂志文章
doi:10.1111/j.1567-1364.2010.00657.x
更新日期:2010-09-01 00:00:00
abstract::The fungus Paracoccidioides brasiliensis causes paracoccidioidomycosis, a systemic granulomatous mycosis prevalent in Latin America. In an effort to elucidate the molecular mechanisms involved in fungus cell wall assembly and morphogenesis, beta-1,3-glucanosyltransferase 3 (PbGel3p) is presented here. PbGel3p presente...
journal_title:FEMS yeast research
pub_type: 杂志文章
doi:10.1111/j.1567-1364.2008.00463.x
更新日期:2009-02-01 00:00:00
abstract::The FLO11-encoded flocculin is required for a variety of important phenotypes in Saccharomyces cerevisiae, including flocculation, adhesion to agar and plastic, invasive growth, pseudohyphae formation and biofilm development. We present evidence that Flo11p belongs to the Flo1-type class of flocculins rather than to t...
journal_title:FEMS yeast research
pub_type: 杂志文章
doi:10.1016/j.femsyr.2005.05.004
更新日期:2005-12-01 00:00:00
abstract::In vitro evaluation of specific anti-Candida albicans sera antibodies based on synthetically prepared complement receptor 3-related protein (CR3-RP) mimicking the structure of native complement receptor 3 in a cohort of 72 patients with atopy and recurrent Candida vulvovaginitis (RVC) revealed effective humoral respon...
journal_title:FEMS yeast research
pub_type: 杂志文章
doi:10.1093/femsyr/fou001
更新日期:2015-03-01 00:00:00
abstract::The presence of 1.0 M KCl or NaCl during growth of Debaryomyces hansenii results in increased ethanol production. An additional increase of fermentation was observed when the salts were also present during incubation under nongrowing conditions. Extracts of cells grown in the presence of salt showed increased alcohol ...
journal_title:FEMS yeast research
pub_type: 杂志文章
doi:10.1111/j.1567-1364.2009.00556.x
更新日期:2009-12-01 00:00:00
abstract::The mechanism involved in the cellular phosphate response of Saccharomyces cerevisiae forms part of the PHO pathway, which upon expression allows a co-ordinated cellular response and adaptation to changes in availability of external phosphate. Although genetic studies and analyses of the S. cerevisiae genome have prod...
journal_title:FEMS yeast research
pub_type: 杂志文章,评审
doi:10.1111/j.1567-1364.2006.00036.x
更新日期:2006-03-01 00:00:00
abstract::We have reported previously that growth on alcohol vapors confers hemolytic properties on certain yeast species and strains ['microbial alcohol-conferred hemolysis' (MACH)]. In a recent study, we analyzed the genetic basis of MACH in Saccharomyces cerevisiae using the EUROSCARF mutant collection. The data suggested th...
journal_title:FEMS yeast research
pub_type: 杂志文章
doi:10.1111/j.1567-1364.2011.00722.x
更新日期:2011-06-01 00:00:00
abstract::Microorganisms have been studied and used extensively to produce value-added fuels and chemicals. Yeasts, specifically Saccharomyces cerevisiae, receive industrial attention because of their well-known ability to ferment glucose and produce ethanol. Thousands of natural or genetically modified S. cerevisiae have been ...
journal_title:FEMS yeast research
pub_type: 杂志文章
doi:10.1093/femsyr/foy001
更新日期:2018-02-01 00:00:00
abstract::Screening for genes affecting super-secreting phenotype of the over-secreting mutant of Kluyveromyces lactis resulted in isolation of the gene named KlMNN10, sharing high homology with Saccharomyces cerevisiae MNN10. The disruption of the KlMNN10 in Kluyveromyces lactis, as well as of MNN10 and MNN11 in Saccharomyces ...
journal_title:FEMS yeast research
pub_type: 杂志文章
doi:10.1016/j.femsyr.2004.03.001
更新日期:2004-09-01 00:00:00
abstract::Yeasts of the genus Candida are a major cause of morbidity and mortality in immunocompromised patients. Despite new insights in recent years, the pathogenesis of Candida infection is still incompletely understood. Previous studies have suggested that gliotoxin, a secondary fungal metabolite with well-known immunosuppr...
journal_title:FEMS yeast research
pub_type: 杂志文章
doi:10.1111/j.1567-1364.2007.00256.x
更新日期:2007-09-01 00:00:00
abstract::Loss of mtDNA by the petite-negative yeast Kluyveromyces lactis is lethal (rho(o)-lethality). However, mutations in the alpha, beta and gamma subunits of F(1)-ATPase can suppress lethality by increasing intramitochondrial hydrolysis of ATP. Increased hydrolysis of ATP can also occur on inactivation of Inh1, the natura...
journal_title:FEMS yeast research
pub_type: 杂志文章
doi:10.1111/j.1567-1364.2006.00201.x
更新日期:2007-08-01 00:00:00
abstract::A salt-sensitive mutant of Kluyveromyces lactis was isolated that was unable to grow in high-salt media. This mutant was also respiratory-deficient and temperature-sensitive for growth. The mutation mapped in a single nuclear gene that is the ortholog of BCS1 of Saccharomyces cerevisiae. The BCS1 product is a mitochon...
journal_title:FEMS yeast research
pub_type: 杂志文章
doi:10.1111/j.1567-1364.2006.00148.x
更新日期:2007-03-01 00:00:00
abstract::Trk1 and Trk2 are the major K(+) transport systems in Schizosaccharomyces pombe. Both transporters individually seem to be able to cope with K(+) requirements of the cells under normal conditions, since only the double mutant shows defective K(+) transport and defective growth at limiting K(+) concentrations. We have ...
journal_title:FEMS yeast research
pub_type: 杂志文章
doi:10.1016/j.femsyr.2003.11.003
更新日期:2004-03-01 00:00:00
abstract::Mutants of Saccharomyces cerevisiae with inactivated endopolyphosphatase gene PPN1 did not grow on lactate and ethanol, and stopped growth on glucose earlier than the parent strain. Their mitochondria were defective in respiration functions and in metabolism of inorganic polyphosphates. The PPN1 mutants lacked exopoly...
journal_title:FEMS yeast research
pub_type: 杂志文章
doi:10.1016/j.femsyr.2005.03.002
更新日期:2005-06-01 00:00:00
abstract::CLC-type chloride/proton antiporters are required for copper/iron homeostasis in fungi. A relationship between CLCs and Ca(2+) homeostasis has not been found before. Here we demonstrate the requirement of the antiporter CLC1 for Ca(2+) homeostasis/signaling in Cryptococcus neoformans. The deletion of CLC1 in JEC21 r...
journal_title:FEMS yeast research
pub_type: 杂志文章
doi:10.1111/j.1567-1364.2011.00763.x
更新日期:2012-02-01 00:00:00
abstract::There is increasing evidence in the literature showing that fungal pathogens express biologically active ectoenzymes. The expression of surface phosphatases at the cell surface of Cryptococcus neoformans, the etiologic agent of cryptococcosis, was evaluated in the present study. Different isolates of C. neoformans exp...
journal_title:FEMS yeast research
pub_type: 杂志文章
doi:10.1111/j.1567-1364.2006.00105.x
更新日期:2006-11-01 00:00:00
abstract::Saccharomyces cerevisiae is an excellent ethanol producer, but is rather sensitive to high concentration of ethanol. Here, influences of ethanol on cellular membrane integrity and carbon metabolism of S. cerevisiae were investigated to rationalize mechanism involved in ethanol toxicity. Addition of 5% (v/v) ethanol di...
journal_title:FEMS yeast research
pub_type: 杂志文章
doi:10.1111/j.1567-1364.2012.00818.x
更新日期:2012-09-01 00:00:00
abstract::First-generation (1G) fuel ethanol production in sugarcane-based biorefineries is an established economic enterprise in Brazil. Second-generation (2G) fuel ethanol from lignocellulosic materials, though extensively investigated, is currently facing severe difficulties to become economically viable. Some of the challen...
journal_title:FEMS yeast research
pub_type: 杂志文章
doi:10.1093/femsyr/foaa027
更新日期:2020-06-01 00:00:00
abstract::Cadmium ions disrupt reactive oxygen species/Ca(2+) homeostasis and subsequently elicit cell death and adaptive signaling cascades in eukaryotic cells. Through a functional genomics approach, we have identified deletion mutants of 106 yeast genes, including three MAP kinase genes (HOG1, SLT2, and KSS1), are sensitive ...
journal_title:FEMS yeast research
pub_type: 杂志文章
doi:10.1111/1567-1364.12220
更新日期:2014-12-01 00:00:00
abstract::In previous communications the essential role of spermidine in Ustilago maydis was demonstrated by means of the disruption of the genes encoding ornithine decarboxylase (ODC) and spermidine synthase (SPE). However, the assignation of specific roles to each polyamine in different cellular functions was not possible bec...
journal_title:FEMS yeast research
pub_type: 杂志文章
doi:10.1111/j.1567-1364.2010.00675.x
更新日期:2010-11-01 00:00:00
abstract::The fungal cell wall is a coherent structure formed by microfibrillar polysaccharides and amorphous material made of other polysaccharides and proteins. We performed a phylogenetic analysis of covalent proteins and enzymes that synthesize fungal wall polysaccharides to determine the possible evolution of the wall stru...
journal_title:FEMS yeast research
pub_type: 杂志文章,评审
doi:10.1111/j.1567-1364.2009.00589.x
更新日期:2010-05-01 00:00:00
abstract::Development of a pulsed-light (PL) approach to inanimate surface decontamination is timely, as the incidence of yeast-related infections in healthcare remains unacceptably high. Critical electrical and biological factors governing the efficacy of PL for the in vitro inactivation of medically important yeast were estab...
journal_title:FEMS yeast research
pub_type: 杂志文章
doi:10.1111/j.1567-1364.2009.00543.x
更新日期:2009-09-01 00:00:00