Abstract：
:The transcriptional response of laboratory strains of Saccharomyces cerevisiae to salt or sorbitol stress has been well studied. These studies have yielded valuable data on how the yeast adapts to these stress conditions. However, S. cerevisiae is a saccharophilic fungus and in its natural environment this yeast encounters high concentrations of sugars. For the production of dessert wines, the sugar concentration may be as high as 50% (w/v). The metabolic pathways in S. cerevisiae under these fermentation conditions have not been studied and the transcriptional response of this yeast to sugar stress has not been investigated. High-density DNA microarrays showed that the transcription of 589 genes in an industrial strain of S. cerevisiae were affected more than two-fold in grape juice containing 40% (w/v) sugars (equimolar amounts of glucose and fructose). High sugar stress up-regulated the glycolytic and pentose phosphate pathway genes. The PDC6 gene, previously thought to encode a minor isozyme of pyruvate decarboxylase, was highly induced under these conditions. Gene expression profiles indicate that the oxidative and non-oxidative branches of the pentose phosphate pathway were up-regulated and might be used to shunt more glucose-6-phosphate and fructose-6-phosphate, respectively, from the glycolytic pathway into the pentose phosphate pathway. Structural genes involved in the formation of acetic acid from acetaldehyde, and succinic acid from glutamate, were also up-regulated. Genes involved in de novo biosynthesis of purines, pyrimidines, histidine and lysine were down-regulated by sugar stress.
journal_name
FEMS Yeast Resjournal_title
FEMS yeast researchauthors
Erasmus DJ,van der Merwe GK,van Vuuren HJdoi
10.1016/S1567-1356(02)00203-9keywords:
subject
Has Abstractpub_date
2003-06-01 00:00:00pages
375-99issue
4eissn
1567-1356issn
1567-1364pii
S1567135602002039journal_volume
3pub_type
杂志文章abstract：:Three ascomycetous yeast strains were isolated from decaying green wine grapes, collected from Hyderabad city in India. Two strains, YS9 and YS21, were identified as Kodamaea ohmeri and Candida fermentati, respectively. The third strain, YS12(T), differs from Candida parapsilosis, Candida orthopsilosis and Candida met...
journal_title：FEMS yeast research
pub_type： 杂志文章
doi：10.1111/j.1567-1364.2006.00206.x
更新日期：2007-05-01 00:00:00
abstract：:Saccharomyces cerevisiae is the most widely used fermentation organism for ethanol production. However, the gene expression regulatory networks behind the ethanol fermentation are still not fully understood. Using a static fermentation model, we examined the ethanol yields on biomass of deletion mutants for 77 yeast g...
journal_title：FEMS yeast research
pub_type： 杂志文章
doi：10.1093/femsyr/fow022
更新日期：2016-05-01 00:00:00
abstract：:Yeast isolates from orange fruit and juice in a spontaneous fermentation were identified and classified by two molecular techniques. The first was analysis of the restriction pattern generated from the polymerase chain reaction (PCR)-amplified 5.8S rRNA gene and the two internal transcribed spacers (ITS) using specifi...
journal_title：FEMS yeast research
pub_type： 杂志文章
doi：10.1111/j.1567-1364.2003.tb00132.x
更新日期：2003-03-01 00:00:00
abstract：:In the macroscopic world, ecological interactions between multiple species of fauna and flora are recognised as major role-players in the evolution of any particular species. By comparison, research on ecological interactions as a driver of evolutionary adaptation in microbial ecosystems has been neglected. The evolut...
journal_title：FEMS yeast research
pub_type： 杂志文章,评审
doi：10.1093/femsyr/foz072
更新日期：2019-11-01 00:00:00
abstract：:The yeast Pichia pastoris is a widely used host organism for heterologous protein production. One of the basic steps for strain improvement is to ensure a sufficient level of transcription of the heterologous gene, based on promoter strength and gene copy number. To date, high-copy-number integrants of P. pastoris are...
journal_title：FEMS yeast research
pub_type： 杂志文章
doi：10.1111/j.1567-1364.2009.00561.x
更新日期：2009-12-01 00:00:00
abstract：:In Kluyveromyces lactis, galactose transport has been thought to be mediated by the lactose permease encoded by LAC12. In fact, a lac12 mutant unable to grow on lactose did not grow on galactose either and showed low and uninducible galactose uptake activity. The existence of other galactose transport systems, at low ...
journal_title：FEMS yeast research
pub_type： 杂志文章
doi：10.1111/j.1567-1364.2006.00107.x
更新日期：2006-12-01 00:00:00
abstract：:Cell volume is an important parameter for modelling cellular processes. Temperature-induced variability of cellular size, volume, intracellular granularity, a fraction of budding cells of yeast Saccharomyces cerevisiae CEN.PK 113-7D (in anaerobic glucose unlimited batch cultures) were measured by flow cytometry and ma...
journal_title：FEMS yeast research
pub_type： 杂志文章
doi：10.1093/femsyr/foy052
更新日期：2018-09-01 00:00:00
abstract：:The transcriptional regulator Pdr1 plays a positive role in regulating azole drug resistance in Candida glabrata. Previous studies have shown the importance of the carboxyl (C)-terminal sequence of Pdr1 in fulfilling its function, as this region mediates interactions between Pdr1 and the co-activator Gal11A and is cru...
journal_title：FEMS yeast research
pub_type： 杂志文章
doi：10.1093/femsyr/foy038
更新日期：2018-06-01 00:00:00
abstract：:The pathogenic yeast Cryptococcus neoformans has evolved several strategies to survive within phagocytes. Recently, it has been demonstrated that upregulation of the ATP binding cassette transporter-encoding gene antifungal resistance 1 (AFR1) is important not only for determining the resistance of C. neoformans to fl...
journal_title：FEMS yeast research
pub_type： 杂志文章
doi：10.1111/j.1567-1364.2008.00470.x
更新日期：2009-03-01 00:00:00
abstract：:A complexity of pathway expression in yeast compared to prokaryotes is the need for separate promoters and terminators for each gene expressed. Single transcript expression and separated protein production is possible via the use of 2A viral peptides, but detailed characterization to assess their suitability and appli...
journal_title：FEMS yeast research
pub_type： 杂志文章
doi：10.1093/femsyr/foy036
更新日期：2018-08-01 00:00:00
abstract：:We developed a method for the quantification of protein turnover rates using (13)C-labeled substrates combined with the analysis of the labeling patterns of proteinogenic amino acids. Using this method, the specific amino acid turnover rates between proteins and the pool of free amino acids were determined for eight d...
journal_title：FEMS yeast research
pub_type： 杂志文章
doi：10.1111/j.1567-1364.2012.00823.x
更新日期：2012-11-01 00:00:00
abstract：:The LYS12 gene from Candida albicans, coding for homoisocitrate dehydrogenase was cloned and expressed as a His-tagged protein in Escherichia coli. The purified gene product catalyzes the Mg(2+)- and K(+)-dependent oxidative decarboxylation of homoisocitrate to α-ketoadipate. The recombinant enzyme demonstrates strict...
journal_title：FEMS yeast research
pub_type： 杂志文章
doi：10.1111/1567-1364.12014
更新日期：2013-03-01 00:00:00
abstract：:Mitochondria are dynamic organelles of endosymbiotic origin that are essential components of eukaryal cells. They contain their own genetic machinery, have multicopy genomes and like their bacterial ancestors they consist of two membranes. However, the majority of the ancestral genome has been lost or transferred to t...
journal_title：FEMS yeast research
pub_type： 杂志文章,评审
doi：10.1093/femsyr/foy040
更新日期：2018-08-01 00:00:00
abstract：:The ultimate goal of therapeutic vaccines is to activate and exploit the patient's own immune system to vigorously and dynamically seek and eradicate established malignant or virally infected cells. Therapeutic vaccines also offer the potential for preventing disease recurrence. Saccharomyces cerevisiae-based vaccines...
journal_title：FEMS yeast research
pub_type： 杂志文章,评审
doi：10.1111/j.1567-1364.2010.00665.x
更新日期：2010-12-01 00:00:00
abstract：:To analyse the reliability and accuracy of genotype analysis with high-density oligonucleotide microarrays, this method and other experimental approaches were used to analyse genomic DNA of two popular Saccharomyces cerevisiae laboratory strains. S288C was used for systematic sequencing of 'the' S. cerevisiae genome; ...
journal_title：FEMS yeast research
pub_type： 杂志文章
doi：10.1016/S1567-1356(03)00156-9
更新日期：2003-12-01 00:00:00
abstract：:Microorganisms have been studied and used extensively to produce value-added fuels and chemicals. Yeasts, specifically Saccharomyces cerevisiae, receive industrial attention because of their well-known ability to ferment glucose and produce ethanol. Thousands of natural or genetically modified S. cerevisiae have been ...
journal_title：FEMS yeast research
pub_type： 杂志文章
doi：10.1093/femsyr/foy001
更新日期：2018-02-01 00:00:00
abstract：:The pathogenic fungus Candida glabrata is relatively resistant to azole antifungals, which target lanosterol 14α-demethylase (Erg11p) in the ergosterol biosynthesis pathway. Our study revealed that C. glabrata exhibits increased azole susceptibility under low-iron conditions. To investigate the molecular basis of this...
journal_title：FEMS yeast research
pub_type： 杂志文章
doi：10.1111/1567-1364.12043
更新日期：2013-06-01 00:00:00
abstract：:Zinc is a crucial mineral for all organisms as it is an essential cofactor for the proper function of a plethora of proteins and depletion of zinc causes oxidative stress. Glutathione is the major redox buffering agent in the cell and therefore important for mitigation of the adverse effects of oxidative stress. In ma...
journal_title：FEMS yeast research
pub_type： 杂志文章
doi：10.1093/femsyr/fox028
更新日期：2017-06-01 00:00:00
abstract：:The FLO11-encoded flocculin is required for a variety of important phenotypes in Saccharomyces cerevisiae, including flocculation, adhesion to agar and plastic, invasive growth, pseudohyphae formation and biofilm development. We present evidence that Flo11p belongs to the Flo1-type class of flocculins rather than to t...
journal_title：FEMS yeast research
pub_type： 杂志文章
doi：10.1016/j.femsyr.2005.05.004
更新日期：2005-12-01 00:00:00
abstract：:Interspecies hybridization is an important evolutionary mechanism in yeasts. The genus Zygosaccharomyces in particular contains numerous hybrid strains and/or species. Here, we investigated the genome of Zygosaccharomyces strain MT15, an isolate from Maotai-flavor Chinese liquor fermentation. We found that it is an in...
journal_title：FEMS yeast research
pub_type： 杂志文章
doi：10.1093/femsyr/foy079
更新日期：2018-11-01 00:00:00
abstract：:Here we present evidence that Hsp150/Pir2, a member of the Pir family of cell wall proteins, can be extracted from the purified cell walls of Saccharomyces cerevisiae by treatment with beta-mercaptoethanol, demonstrating that at least part of this protein is attached to the cell wall through disulfide bridges. We also...
journal_title：FEMS yeast research
pub_type： 杂志文章
doi：10.1111/j.1567-1364.2001.tb00040.x
更新日期：2001-12-01 00:00:00
abstract：:Aldose-1-epimerase or mutarotase (EC 5.1.3.3) catalyzes interconversion of alpha/beta-anomers of aldoses, such as glucose and galactose, and is distributed in a wide variety of organisms from bacteria to humans. Nevertheless, the physiological role of this enzyme has been elusive in most cases, because the alpha-form ...
journal_title：FEMS yeast research
pub_type： 杂志文章
doi：10.1111/j.1567-1364.2009.00517.x
更新日期：2009-08-01 00:00:00
abstract：:Genes of the post-squalene ergosterol biosynthetic pathway in Saccharomyces cerevisiae have been overexpressed in a systematic approach with the aim to construct yeast strains that produce high amounts of sterols from a squalene-accumulating strain. This strain had previously been deregulated by overexpressing a trunc...
journal_title：FEMS yeast research
pub_type： 杂志文章
doi：10.1016/S1567-1356(03)00126-0
更新日期：2003-10-01 00:00:00
abstract：:Penicillin G amidase from Providencia rettgeri is a heterodimer of 92 kDa. We have previously expressed the Pr. rettgeri pac gene coding for this enzyme in Saccharomyces cerevisiae, and now we report the expression and characterization in the methylotrophic yeast Pichia pastoris. The recombinant catalytically active e...
journal_title：FEMS yeast research
pub_type： 杂志文章
doi：10.1111/j.1567-1364.2002.tb00045.x
更新日期：2002-01-01 00:00:00
abstract：:Candida lipolytica and Candida rugosa were isolated from blood samples from a patient with chronic myeloid leukemia (31 years old) and a patient with sickle cell disease (1-year-old), respectively. Isolates were grown for 48 h at 37 degrees C in either Sabouraud or tryptone soy broth (TSB). Peptidases were tested for ...
journal_title：FEMS yeast research
pub_type： 杂志文章
doi：10.1111/j.1567-1364.2007.00269.x
更新日期：2007-09-01 00:00:00
abstract：:Differences in glycosylation between the natural alpha-1,6 glucan-6-glucanohydrolase from Penicillium minioluteum and the heterologous protein expressed in the yeast Pichia pastoris were analyzed. Glycosylation profiling was carried out using fluorophore-assisted carbohydrate electrophoresis and amine absorption high-...
journal_title：FEMS yeast research
pub_type： 杂志文章
doi：10.1111/j.1567-1364.2001.tb00026.x
更新日期：2001-07-01 00:00:00
abstract：:Yarrowia lipolytica is a dimorphic fungus whose morphology is controlled by several factors such as pH and different compounds. To determine if the STE11-mitogen-activated protein kinase (MAPK) pathway plays a role in dimorphism of Y. lipolytica, we isolated the gene encoding a Mapkkk. The isolated gene (STE11) has an...
journal_title：FEMS yeast research
pub_type： 杂志文章
doi：10.1111/j.1567-1364.2006.00084.x
更新日期：2006-08-01 00:00:00
abstract：:Fungal wall formation is a dynamic process involving several categories of enzymes. The GH72 family of β(1,3)-glucanosyltransferases is essential for the determination of cell shape, for cell integrity and for virulence in pathogenic fungi. Candida albicans has five GH72 genes: PHR1 and PHR2 are pH dependent, the firs...
journal_title：FEMS yeast research
pub_type： 杂志文章
doi：10.1093/femsyr/fou011
更新日期：2015-03-01 00:00:00
abstract：:Homologous recombination (HR) is an error-free DNA repair mechanism that maintains genome integrity by repairing double-strand breaks (DSBs). Defects in HR lead to genomic instability and are associated with cancer predisposition. A key step in HR is the formation of Rad51 nucleoprotein filaments which are responsible...
journal_title：FEMS yeast research
pub_type： 杂志文章
doi：10.1093/femsyr/fow073
更新日期：2016-09-01 00:00:00
abstract：:In previous communications the essential role of spermidine in Ustilago maydis was demonstrated by means of the disruption of the genes encoding ornithine decarboxylase (ODC) and spermidine synthase (SPE). However, the assignation of specific roles to each polyamine in different cellular functions was not possible bec...
journal_title：FEMS yeast research
pub_type： 杂志文章
doi：10.1111/j.1567-1364.2010.00675.x
更新日期：2010-11-01 00:00:00
