Impact of glutathione metabolism on zinc homeostasis in Saccharomyces cerevisiae.

abstract：:An extended definition of the term metabolic engineering is given and its successful use in the construction of biorecognition elements of sensors is demonstrated. It is shown that genetic and chemical modifications of methylotrophic yeast cells provide directed changes in their physiological responses towards methano...

journal_title：FEMS yeast research

authors： Gonchar M,Maidan M,Korpan Y,Sibirny V,Kotylak Z,Sibirny A

更新日期：2002-08-01 00:00:00

abstract：:A biofilm-forming strain of Pichia fermentans proved to be most effective in controlling brown rot on apple fruit when coinoculated into artificial wounds with a phytopathogenic isolate of Monilinia fructicola. Culture filtrates and autoclaved cells had no significant influence on the disease. When sprayed onto the ap...

journal_title：FEMS yeast research

authors： Giobbe S,Marceddu S,Scherm B,Zara G,Mazzarello VL,Budroni M,Migheli Q

更新日期：2007-12-01 00:00:00

abstract：:Genes of the post-squalene ergosterol biosynthetic pathway in Saccharomyces cerevisiae have been overexpressed in a systematic approach with the aim to construct yeast strains that produce high amounts of sterols from a squalene-accumulating strain. This strain had previously been deregulated by overexpressing a trunc...

journal_title：FEMS yeast research

authors： Veen M,Stahl U,Lang C

更新日期：2003-10-01 00:00:00

abstract：:The opportunistic human fungal pathogen Candida glabrata is second only to C. albicans as the cause of Candida infections and yet is more closely related to Saccharomyces cerevisiae. Recent advances in functional genomics technologies and computational approaches to decipher regulatory networks, and the comparison of ...

journal_title：FEMS yeast research

authors： Roy S,Thompson D

更新日期：2015-12-01 00:00:00

abstract：:Using modern peptide analytical MS technology ('Peptidomics'), it is possible to analyze yeast α-pheromone both qualitatively and semi-quantitatively directly from conditioned cell culture media. MS/MS analysis shows both forms of α-pheromone (MFα and MFα') detectable and identifiable straight from WT supernatants. In...

journal_title：FEMS yeast research

authors： Bener Aksam E,Pinkse MW,Verhaert PD

更新日期：2013-05-01 00:00:00

abstract：:The vast majority of wine fermentations are performed principally by Saccharomyces cerevisiae. However, there are a growing number of instances in which other species of Saccharomyces play a predominant role. Interestingly, the presence of these other yeast species generally occurs via the formation of interspecific h...

journal_title：FEMS yeast research

authors： Borneman AR,Desany BA,Riches D,Affourtit JP,Forgan AH,Pretorius IS,Egholm M,Chambers PJ

更新日期：2012-02-01 00:00:00

abstract：:CLC-type chloride/proton antiporters are required for copper/iron homeostasis in fungi. A relationship between CLCs and Ca(2+) homeostasis has not been found before. Here we demonstrate the requirement of the antiporter CLC1 for Ca(2+) homeostasis/signaling in Cryptococcus neoformans. The deletion of CLC1 in JEC21 r...

journal_title：FEMS yeast research

authors： Li D,Zhang X,Li Z,Yang J,Pan J,Zhu X

更新日期：2012-02-01 00:00:00

abstract：:Microorganisms have been studied and used extensively to produce value-added fuels and chemicals. Yeasts, specifically Saccharomyces cerevisiae, receive industrial attention because of their well-known ability to ferment glucose and produce ethanol. Thousands of natural or genetically modified S. cerevisiae have been ...

journal_title：FEMS yeast research

authors： Kong II,Turner TL,Kim H,Kim SR,Jin YS

更新日期：2018-02-01 00:00:00

abstract：:The LYS12 gene from Candida albicans, coding for homoisocitrate dehydrogenase was cloned and expressed as a His-tagged protein in Escherichia coli. The purified gene product catalyzes the Mg(2+)- and K(+)-dependent oxidative decarboxylation of homoisocitrate to α-ketoadipate. The recombinant enzyme demonstrates strict...

journal_title：FEMS yeast research

authors： Gabriel I,Vetter ND,Palmer DR,Milewska MJ,Wojciechowski M,Milewski S

更新日期：2013-03-01 00:00:00

abstract：:During their mitotic cycle, cylindrical fission yeast cells grow exclusively at their tips. Length growth starts at birth and halts at mitotic onset when the cells begin to prepare for division. While the growth pattern was initially considered to be exponential, during the last three decades an increasing amount of e...

journal_title：FEMS yeast research

authors： Horváth A,Rácz-Mónus A,Buchwald P,Sveiczer Á

更新日期：2013-11-01 00:00:00

abstract：:The fungal cell wall is a coherent structure formed by microfibrillar polysaccharides and amorphous material made of other polysaccharides and proteins. We performed a phylogenetic analysis of covalent proteins and enzymes that synthesize fungal wall polysaccharides to determine the possible evolution of the wall stru...

journal_title：FEMS yeast research

authors： Ruiz-Herrera J,Ortiz-Castellanos L

更新日期：2010-05-01 00:00:00

abstract：:Analysis of nucleotide sequences from the domains 1 and 2 of the large-subunit rDNA demonstrated species of the Pichia fluxuum clade to be phylogenetically isolated, and domains 1 and 2 and internal transcribed spacer rDNA sequence analyses of strains phenotypically identified as P. fluxuum resulted in the discovery o...

journal_title：FEMS yeast research

authors： Kurtzman CP

更新日期：2006-03-01 00:00:00

abstract：:In Saccharomyces cerevisiae, the cell integrity pathway plays a role in the oxidative stress response. In this study, we show that the Pkc1 protein mediates oxidative signalling by helping to downregulate ribosomal gene expression when cells are exposed to hydrogen peroxide. An active actin cytoskeleton is required fo...

journal_title：FEMS yeast research

authors： Mitjana FV,Petkova MI,Pujol-Carrion N,de la Torre-Ruiz MA

更新日期：2011-12-01 00:00:00

abstract：:Xylose fermentation is a rare trait that is immensely important to the cellulosic biofuel industry, and Candida tenuis is one of the few yeasts that has been reported with this trait. Here we report the isolation of two strains representing a candidate sister species to C. tenuis. Integrated analysis of genome sequenc...

journal_title：FEMS yeast research

authors： Haase MAB,Kominek J,Langdon QK,Kurtzman CP,Hittinger CT

更新日期：2017-05-01 00:00:00

abstract：:Screening for genes affecting super-secreting phenotype of the over-secreting mutant of Kluyveromyces lactis resulted in isolation of the gene named KlMNN10, sharing high homology with Saccharomyces cerevisiae MNN10. The disruption of the KlMNN10 in Kluyveromyces lactis, as well as of MNN10 and MNN11 in Saccharomyces ...

journal_title：FEMS yeast research

authors： Bartkeviciūte D,Sasnauskas K

更新日期：2004-09-01 00:00:00

abstract：:The durum wheat TMKP1 gene encodes a MAP kinase phosphatase. When overexpressed in Saccharomyces cerevisiae, TMKP1 leads to salt stress tolerance (especially LiCl ), which is dependent on the phosphatase activity of the protein. The TMKP1-associated Li(+) resistance is restricted to a galactose-containing medium. Inte...

journal_title：FEMS yeast research

authors： Zaidi I,González A,Touzri M,Alvarez MC,Ramos J,Masmoudi K,Ariño J,Hanin M

更新日期：2012-11-01 00:00:00

abstract：:In the macroscopic world, ecological interactions between multiple species of fauna and flora are recognised as major role-players in the evolution of any particular species. By comparison, research on ecological interactions as a driver of evolutionary adaptation in microbial ecosystems has been neglected. The evolut...

journal_title：FEMS yeast research

authors： Conacher CG,Rossouw D,Bauer FFB

更新日期：2019-11-01 00:00:00

abstract：:Candida albicans maintains genetic diversity by random chromosome alterations, and this diversity allows utilization of various nutrients. Although the alterations seem to occur spontaneously, their frequencies clearly depend on environmental factors. In addition, this microorganism survives in adverse environments, w...

journal_title：FEMS yeast research

authors： Rustchenko E

更新日期：2007-01-01 00:00:00

abstract：:We used multi-locus sequence typing (MLST) to investigate 35 yeast isolates representing the two genome-sequenced strains plus the type strain of Candida albicans, four isolates originally identified as Candida stellatoidea type I and 28 representing type strains of other species now regarded as synonymous with C. alb...

journal_title：FEMS yeast research

authors： Jacobsen MD,Boekhout T,Odds FC

更新日期：2008-08-01 00:00:00

abstract：:First-generation (1G) fuel ethanol production in sugarcane-based biorefineries is an established economic enterprise in Brazil. Second-generation (2G) fuel ethanol from lignocellulosic materials, though extensively investigated, is currently facing severe difficulties to become economically viable. Some of the challen...

journal_title：FEMS yeast research

authors： Bermejo PM,Raghavendran V,Gombert AK

更新日期：2020-06-01 00:00:00

abstract：:Previously, we reported that Aft1 regulates Sit1 by modulating the ubiquitination of Sit1 in Saccharomyces cerevisiae. Here, we report the function of the physical interaction between Sit1 and Aft1 in ferrioxamine B (FOB) uptake. The interaction between Sit1 and Aft1 induced protein localization of Sit1 to the plasma ...

journal_title：FEMS yeast research

authors： Kang CM,Kang S,Park YS,Yun CW

更新日期：2015-11-01 00:00:00

abstract：:Yeast isolates from orange fruit and juice in a spontaneous fermentation were identified and classified by two molecular techniques. The first was analysis of the restriction pattern generated from the polymerase chain reaction (PCR)-amplified 5.8S rRNA gene and the two internal transcribed spacers (ITS) using specifi...

journal_title：FEMS yeast research

authors： Las Heras-Vazquez FJ,Mingorance-Cazorla L,Clemente-Jimenez JM,Rodriguez-Vico F

更新日期：2003-03-01 00:00:00

abstract：:Killer-toxin complexes produced by Kluyveromyces lactis and Pichia acaciae inhibit cell proliferation of Saccharomyces cerevisiae. Analysis of their actions in haploid MATalpha cells revealed that introduction of the opposite mating-type locus (MATa) significantly suppressed antizymosis. Together with resistance expre...

journal_title：FEMS yeast research

authors： Klassen R,Jablonowski D,Stark MJ,Schaffrath R,Meinhardt F

更新日期：2006-05-01 00:00:00

abstract：:The FLO11-encoded flocculin is required for a variety of important phenotypes in Saccharomyces cerevisiae, including flocculation, adhesion to agar and plastic, invasive growth, pseudohyphae formation and biofilm development. We present evidence that Flo11p belongs to the Flo1-type class of flocculins rather than to t...

journal_title：FEMS yeast research

authors： Bayly JC,Douglas LM,Pretorius IS,Bauer FF,Dranginis AM

更新日期：2005-12-01 00:00:00

abstract：:Candida glabrata is a second most common human opportunistic pathogen which causes superficial but also life-threatening systemic candidosis. According to the localisation of mannans and mannoproteins in the outermost layer of the cell wall, mannan detection could be one of the first steps in the cell recognition of C...

journal_title：FEMS yeast research

authors： Paulovičová L,Paulovičová E,Farkaš P,Čížová A,Bystrický P,Jančinová V,Turánek J,Pericolini E,Gabrielli E,Vecchiarelli A,Hrubiško M

更新日期：2019-03-01 00:00:00

abstract：:The most diffused formulation of starter for winemaking is active dry yeast (ADY). ADYs production process is essentially characterized by air-drying stress, a combination of several stresses, including thermal, hyperosmotic and oxidative and cell capacity to counteract such multiple stresses will determine its surviv...

journal_title：FEMS yeast research

authors： Capece A,Votta S,Guaragnella N,Zambuto M,Romaniello R,Romano P

更新日期：2016-05-01 00:00:00

abstract：:The asci of Metschnikowia species normally contain two ascospores (never more), raising the question of whether these spores are true meiotic products. We investigated this problem by crossing genetically-marked strains of the haploid, heterothallic taxa Metschnikowia hawaiiensis, Metschnikowia continentalis var. cont...

journal_title：FEMS yeast research

authors： Marinoni G,Piskur J,Lachance MA

更新日期：2003-03-01 00:00:00

abstract：:Aldose-1-epimerase or mutarotase (EC 5.1.3.3) catalyzes interconversion of alpha/beta-anomers of aldoses, such as glucose and galactose, and is distributed in a wide variety of organisms from bacteria to humans. Nevertheless, the physiological role of this enzyme has been elusive in most cases, because the alpha-form ...

journal_title：FEMS yeast research

authors： Fukasawa T,Sakurai H,Nogi Y,Baruffini E

更新日期：2009-08-01 00:00:00

abstract：:Formic acid disrupts mitochondrial electron transport and sequentially causes cell death in mammalian ocular cells by an unidentified molecular mechanism. Here, we show that a low concentration of formic acid induces apoptosis-like cell death in the budding yeast Saccharomyces cerevisiae, with several morphological an...

journal_title：FEMS yeast research

authors： Du L,Su Y,Sun D,Zhu W,Wang J,Zhuang X,Zhou S,Lu Y

更新日期：2008-06-01 00:00:00

abstract：:Nine yeast strains were isolated from spontaneous fermentations in the Alsace area of France, during the 1997, 1998 and 1999 grape harvests. Strains were characterized by pulsed-field gel electrophoresis, PCR-restriction fragment length polymorphism (RFLP) of the MET2 gene, delta-PCR, and microsatellite patterns. Kary...

journal_title：FEMS yeast research

authors： Le Jeune C,Lollier M,Demuyter C,Erny C,Legras JL,Aigle M,Masneuf-Pomarède I

更新日期：2007-06-01 00:00:00