Abstract：
:The most diffused formulation of starter for winemaking is active dry yeast (ADY). ADYs production process is essentially characterized by air-drying stress, a combination of several stresses, including thermal, hyperosmotic and oxidative and cell capacity to counteract such multiple stresses will determine its survival. The molecular mechanisms underlying cell stress response to desiccation have been mostly studied in laboratory and commercial yeast strains, but a growing interest is currently developing for indigenous yeast strains which represent a valuable and alternative source of genetic and molecular biodiversity to be exploited. In this work, a comparative study of different Saccharomyces cerevisiae indigenous wine strains, previously selected for their technological traits, has been carried out to identify potentially relevant genes involved in desiccation stress tolerance. Cell viability was evaluated along desiccation treatment and gene expression was analyzed by real-time PCR before and during the stress. Our data show that the observed differences in individual strain sensitivity to desiccation stress could be associated to specific gene expression over time. In particular, either the basal or the stress-induced mRNA levels of certain genes, such as HSP12, SSA3, TPS1, TPS2, CTT1 and SOD1, result tightly correlated to the strain survival advantage. This study provides a reliable and sensitive method to predict desiccation stress tolerance of indigenous wine yeast strains which could be preliminary to biotechnological applications.
journal_name
FEMS Yeast Resjournal_title
FEMS yeast researchauthors
Capece A,Votta S,Guaragnella N,Zambuto M,Romaniello R,Romano Pdoi
10.1093/femsyr/fow015subject
Has Abstractpub_date
2016-05-01 00:00:00issue
3eissn
1567-1356issn
1567-1364pii
fow015journal_volume
16pub_type
杂志文章abstract：:Mitochondria are responsible for a series of metabolic functions. Superoxide leakage from the respiratory chain and the resulting cascade of reactive oxygen species-induced damage, as well as mitochondrial metabolism in programmed cell death, have been intensively studied during ageing in single-cellular and higher or...
journal_title：FEMS yeast research
pub_type： 杂志文章,评审
doi：10.1111/1567-1364.12134
更新日期：2014-02-01 00:00:00
abstract：:Saccharomyces cerevisiae is an excellent ethanol producer, but is rather sensitive to high concentration of ethanol. Here, influences of ethanol on cellular membrane integrity and carbon metabolism of S. cerevisiae were investigated to rationalize mechanism involved in ethanol toxicity. Addition of 5% (v/v) ethanol di...
journal_title：FEMS yeast research
pub_type： 杂志文章
doi：10.1111/j.1567-1364.2012.00818.x
更新日期：2012-09-01 00:00:00
abstract：:Microorganisms have been studied and used extensively to produce value-added fuels and chemicals. Yeasts, specifically Saccharomyces cerevisiae, receive industrial attention because of their well-known ability to ferment glucose and produce ethanol. Thousands of natural or genetically modified S. cerevisiae have been ...
journal_title：FEMS yeast research
pub_type： 杂志文章
doi：10.1093/femsyr/foy001
更新日期：2018-02-01 00:00:00
abstract：:Killer-toxin complexes produced by Kluyveromyces lactis and Pichia acaciae inhibit cell proliferation of Saccharomyces cerevisiae. Analysis of their actions in haploid MATalpha cells revealed that introduction of the opposite mating-type locus (MATa) significantly suppressed antizymosis. Together with resistance expre...
journal_title：FEMS yeast research
pub_type： 杂志文章
doi：10.1111/j.1567-1364.2005.00006.x
更新日期：2006-05-01 00:00:00
abstract：:A robust cell factory that can tolerate combined inhibitory lignocellulosic compounds is essential for the cost-effective lignocellulose-based production of second-generation bioethanol and other bulk chemicals. Following high-throughput phenotyping of a yeast genomic overexpression library, we identified a Saccharomy...
journal_title：FEMS yeast research
pub_type： 杂志文章
doi：10.1093/femsyr/foy106
更新日期：2018-12-01 00:00:00
abstract：:Coenzyme Q is a redox-active lipid that functions as an electron carrier in the mitochondrial respiratory chain. Q-biosynthesis in Saccharomyces cerevisiae requires at least nine proteins (Coq1p-Coq9p). The molecular function of Coq8p is still unknown; however, lack of Q and the concomitant accumulation of the interme...
journal_title：FEMS yeast research
pub_type： 杂志文章
doi：10.1111/j.1567-1364.2008.00436.x
更新日期：2008-12-01 00:00:00
abstract：:In the macroscopic world, ecological interactions between multiple species of fauna and flora are recognised as major role-players in the evolution of any particular species. By comparison, research on ecological interactions as a driver of evolutionary adaptation in microbial ecosystems has been neglected. The evolut...
journal_title：FEMS yeast research
pub_type： 杂志文章,评审
doi：10.1093/femsyr/foz072
更新日期：2019-11-01 00:00:00
abstract：:Fungal wall formation is a dynamic process involving several categories of enzymes. The GH72 family of β(1,3)-glucanosyltransferases is essential for the determination of cell shape, for cell integrity and for virulence in pathogenic fungi. Candida albicans has five GH72 genes: PHR1 and PHR2 are pH dependent, the firs...
journal_title：FEMS yeast research
pub_type： 杂志文章
doi：10.1093/femsyr/fou011
更新日期：2015-03-01 00:00:00
abstract：:The budding yeast, Saccharomyces cerevisiae, is an efficient model for studying oxidative stress, programmed cell death and aging. The present study was carried out to investigate antioxidant, the anti-apoptotic and anti-aging activity of a natural compound, astaxanthin, in S. cerevisiae model. The survivability of ye...
journal_title：FEMS yeast research
pub_type： 杂志文章
doi：10.1093/femsyr/foy113
更新日期：2019-01-01 00:00:00
abstract：:We show that Arf3p, a member of the ADP ribosylation family, is involved in the organization of actin cables and cortical patches in Saccharomyces cerevisiae. Profilin-deficient cells (pfy1Delta) have severe growth defects and lack actin cables. Overexpression of ARF3 restores actin cables and corrects growth defects ...
journal_title：FEMS yeast research
pub_type： 杂志文章
doi：10.1111/j.1567-1364.2007.00239.x
更新日期：2007-09-01 00:00:00
abstract：:Current paradigms assume that gas bubbles cannot be formed within yeasts although these workhorses of the baking and brewing industries vigorously produce and release CO(2) gas. We show that yeasts produce gas bubbles that fill a significant part of the cell. The missing link between intracellular CO(2) production b...
journal_title：FEMS yeast research
pub_type： 杂志文章
doi：10.1111/j.1567-1364.12004.x
更新日期：2012-11-01 00:00:00
abstract：:Yarrowia lipolytica is a dimorphic fungus whose morphology is controlled by several factors such as pH and different compounds. To determine if the STE11-mitogen-activated protein kinase (MAPK) pathway plays a role in dimorphism of Y. lipolytica, we isolated the gene encoding a Mapkkk. The isolated gene (STE11) has an...
journal_title：FEMS yeast research
pub_type： 杂志文章
doi：10.1111/j.1567-1364.2006.00084.x
更新日期：2006-08-01 00:00:00
abstract：:Loss of mtDNA by the petite-negative yeast Kluyveromyces lactis is lethal (rho(o)-lethality). However, mutations in the alpha, beta and gamma subunits of F(1)-ATPase can suppress lethality by increasing intramitochondrial hydrolysis of ATP. Increased hydrolysis of ATP can also occur on inactivation of Inh1, the natura...
journal_title：FEMS yeast research
pub_type： 杂志文章
doi：10.1111/j.1567-1364.2006.00201.x
更新日期：2007-08-01 00:00:00
abstract：:The presence of 1.0 M KCl or NaCl during growth of Debaryomyces hansenii results in increased ethanol production. An additional increase of fermentation was observed when the salts were also present during incubation under nongrowing conditions. Extracts of cells grown in the presence of salt showed increased alcohol ...
journal_title：FEMS yeast research
pub_type： 杂志文章
doi：10.1111/j.1567-1364.2009.00556.x
更新日期：2009-12-01 00:00:00
abstract：:Here we present evidence that Hsp150/Pir2, a member of the Pir family of cell wall proteins, can be extracted from the purified cell walls of Saccharomyces cerevisiae by treatment with beta-mercaptoethanol, demonstrating that at least part of this protein is attached to the cell wall through disulfide bridges. We also...
journal_title：FEMS yeast research
pub_type： 杂志文章
doi：10.1111/j.1567-1364.2001.tb00040.x
更新日期：2001-12-01 00:00:00
abstract：:The expression of the transcription regulator Pdr1p and its target genes PDR5 and TPO1 is required for Saccharomyces cerevisiae adaptation and resistance to artesunate, a promising antimalarial drug, also active against tumour cells and viruses. PDR5 and TPO1 encode plasma membrane multidrug transporters of the ATP-bi...
journal_title：FEMS yeast research
pub_type： 杂志文章
doi：10.1111/j.1567-1364.2006.00095.x
更新日期：2006-12-01 00:00:00
abstract：:Maintenance and adaptation of energy metabolism could play an important role in the cellular ability to respond to DNA damage. A large number of studies suggest that the sensitivity of cells to oxidants and oxidative stress depends on the activity of cellular metabolism and is dependent on the glucose concentration. I...
journal_title：FEMS yeast research
pub_type： 杂志文章
doi：10.1111/j.1567-1364.2009.00505.x
更新日期：2009-06-01 00:00:00
abstract：:Fourteen indigenous Saccharomyces cerevisiae strains isolated from the barks of three tree species located in the Atlantic Rain Forest and Cerrado biomes in Brazil were genetically and physiologically compared to laboratory strains and to strains from the Brazilian fuel ethanol industry. Although no clear correlation ...
journal_title：FEMS yeast research
pub_type： 杂志文章
doi：10.1093/femsyr/fow076
更新日期：2016-11-01 00:00:00
abstract：:Saccharomyces cerevisiae yeast strains exhibit a huge genotypic and phenotypic diversity. Breeding strategies taking advantage of these characteristics would contribute greatly to improving industrial yeasts. Here we mapped and introgressed chromosomal regions controlling industrial yeast properties, such as hydrogen ...
journal_title：FEMS yeast research
pub_type： 杂志文章
doi：10.1111/j.1567-1364.2007.00281.x
更新日期：2007-12-01 00:00:00
abstract：:Candida lipolytica and Candida rugosa were isolated from blood samples from a patient with chronic myeloid leukemia (31 years old) and a patient with sickle cell disease (1-year-old), respectively. Isolates were grown for 48 h at 37 degrees C in either Sabouraud or tryptone soy broth (TSB). Peptidases were tested for ...
journal_title：FEMS yeast research
pub_type： 杂志文章
doi：10.1111/j.1567-1364.2007.00269.x
更新日期：2007-09-01 00:00:00
abstract：:Since the discovery of the apoptotic pathway in Saccharomyces cerevisiae, several compounds have been shown to cause apoptosis in this organism. While the toxicity of polyunsaturated fatty acids (PUFA) peroxides towards S. cerevisiae has been known for a long time, studies on the effect of nonoxidized PUFA are scarce....
journal_title：FEMS yeast research
pub_type： 杂志文章
doi：10.1111/j.1567-1364.2011.00729.x
更新日期：2011-08-01 00:00:00
abstract：:Murine experimentation has provided many useful tools, including the ability to knockout or over-express genes and to perform experiments that are limited by ethical considerations. Over the past century, mice have imparted valuable insights into the biology of many systems, including human immunity. However, although...
journal_title：FEMS yeast research
pub_type： 杂志文章,评审
doi：10.1111/j.1567-1364.2006.00096.x
更新日期：2006-06-01 00:00:00
abstract：:During their mitotic cycle, cylindrical fission yeast cells grow exclusively at their tips. Length growth starts at birth and halts at mitotic onset when the cells begin to prepare for division. While the growth pattern was initially considered to be exponential, during the last three decades an increasing amount of e...
journal_title：FEMS yeast research
pub_type： 杂志文章
doi：10.1111/1567-1364.12064
更新日期：2013-11-01 00:00:00
abstract：:Amylases are used in a variety of industries that have a specific need for alternative enzymes capable of hydrolysing raw starch. Five α-amylase and five glucoamylase-encoding genes were expressed in the Saccharomyces cerevisiae Y294 laboratory strain to select for recombinant strains that best hydrolysed raw corn sta...
journal_title：FEMS yeast research
pub_type： 杂志文章
doi：10.1093/femsyr/foy127
更新日期：2019-03-01 00:00:00
abstract：:The opportunistic human fungal pathogen Candida glabrata is second only to C. albicans as the cause of Candida infections and yet is more closely related to Saccharomyces cerevisiae. Recent advances in functional genomics technologies and computational approaches to decipher regulatory networks, and the comparison of ...
journal_title：FEMS yeast research
pub_type： 杂志文章,评审
doi：10.1093/femsyr/fov087
更新日期：2015-12-01 00:00:00
abstract：:The fungal cell wall is a coherent structure formed by microfibrillar polysaccharides and amorphous material made of other polysaccharides and proteins. We performed a phylogenetic analysis of covalent proteins and enzymes that synthesize fungal wall polysaccharides to determine the possible evolution of the wall stru...
journal_title：FEMS yeast research
pub_type： 杂志文章,评审
doi：10.1111/j.1567-1364.2009.00589.x
更新日期：2010-05-01 00:00:00
abstract：:Each time a cell duplicates, the whole genome must be accurately copied and distributed. The enormous amount of DNA in eukaryotic cells requires a high level of coordination between polymerases and other DNA and chromatin-interacting proteins to ensure timely and accurate DNA replication and chromatin formation. PCNA ...
journal_title：FEMS yeast research
pub_type： 杂志文章,评审
doi：10.1093/femsyr/fow084
更新日期：2016-11-01 00:00:00
abstract：:The pathogenic yeast Cryptococcus neoformans has evolved several strategies to survive within phagocytes. Recently, it has been demonstrated that upregulation of the ATP binding cassette transporter-encoding gene antifungal resistance 1 (AFR1) is important not only for determining the resistance of C. neoformans to fl...
journal_title：FEMS yeast research
pub_type： 杂志文章
doi：10.1111/j.1567-1364.2008.00470.x
更新日期：2009-03-01 00:00:00
abstract：:Saccharomyces cerevisiae grown on plastic surfaces formed organized structures, termed minicolonies, that consisted of a core of round (yeast-like) cells surrounded by chains of filamentous cells (pseudohyphae). Minicolonies had a much higher affinity for plastic than unstructured yeast communities growing on the same...
journal_title：FEMS yeast research
pub_type： 杂志文章
doi：10.1111/j.1567-1364.2010.00712.x
更新日期：2011-03-01 00:00:00
abstract：:Interspecies hybridization is an important evolutionary mechanism in yeasts. The genus Zygosaccharomyces in particular contains numerous hybrid strains and/or species. Here, we investigated the genome of Zygosaccharomyces strain MT15, an isolate from Maotai-flavor Chinese liquor fermentation. We found that it is an in...
journal_title：FEMS yeast research
pub_type： 杂志文章
doi：10.1093/femsyr/foy079
更新日期：2018-11-01 00:00:00
