Abstract:
:The expression of the transcription regulator Pdr1p and its target genes PDR5 and TPO1 is required for Saccharomyces cerevisiae adaptation and resistance to artesunate, a promising antimalarial drug, also active against tumour cells and viruses. PDR5 and TPO1 encode plasma membrane multidrug transporters of the ATP-binding cassette and the major facilitator superfamilies, respectively. The transcriptional activation of TPO1 (10-fold) and PDR5 (13-fold) was registered after 30 min of exposure of the unadapted yeast population to acute artesunate-induced stress, being significantly reduced in the absence of Pdr1p and abolished in the absence of Pdr1p and Pdr3p. Maximum TPO1 mRNA levels were rapidly reduced to basal values following adaptation of the yeast population to artesunate, while high PDR5 levels were maintained during drug-stressed exponential growth.
journal_name
FEMS Yeast Resjournal_title
FEMS yeast researchauthors
Alenquer M,Tenreiro S,Sá-Correia Idoi
10.1111/j.1567-1364.2006.00095.xsubject
Has Abstractpub_date
2006-12-01 00:00:00pages
1130-9issue
8eissn
1567-1356issn
1567-1364pii
FYR095journal_volume
6pub_type
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