Abstract:
:Candida albicans is an important opportunistic yeast pathogen of humans and has the ability to form drug-resistant biofilms, with increased expression of multidrug ATP-binding cassette (ABC) transporters. These biofilms are also capable of secreting immune-modulating prostaglandin E2 (PGE2 ) from host-derived arachidonic acid (AA). Phenothiazine, an aromatic amine, and its derivatives display broad activity as inhibitors and antioxidants. These compounds have fungistatic and fungicidal activity against planktonic C. albicans and can inhibit ABC transporters of C. albicans. This study investigated the effect of phenothiazine on biofilm formation, ABC transporters and PGE2 production by C. albicans. This was carried out by growing C. albicans biofilms in the presence of AA and phenothiazine and measuring the biomass as well as reduction of 2,3-bis(2-methoxy-4-nitro-5-sulphophenyl)-5[(phenylamino) carbonyl]-2H tetrazolium hydroxide. The effect on ABC transporters was determined by rhodamine 6G efflux, and the concentration of PGE2 was determined by a monoclonal PGE2 enzyme-linked immunosorbent assay and LC/MS/MS. Our results indicate that phenothiazine can cause a reduction in both the metabolic activity and biomass of C. albicans biofilms, without affecting biofilm morphology or ABC transporters. However, it is a potent inhibitor of PGE2 production by C. albicans biofilms.
journal_name
FEMS Yeast Resjournal_title
FEMS yeast researchauthors
Ells R,Kemp G,Albertyn J,Kock JL,Pohl CHdoi
10.1111/1567-1364.12093subject
Has Abstractpub_date
2013-12-01 00:00:00pages
849-55issue
8eissn
1567-1356issn
1567-1364journal_volume
13pub_type
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journal_title:FEMS yeast research
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journal_title:FEMS yeast research
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pub_type: 杂志文章
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更新日期:2019-12-01 00:00:00
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journal_title:FEMS yeast research
pub_type: 杂志文章,评审
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