Metabolically engineered methylotrophic yeast cells and enzymes as sensor biorecognition elements.

abstract：:In Saccharomyces cerevisiae, the cell integrity pathway plays a role in the oxidative stress response. In this study, we show that the Pkc1 protein mediates oxidative signalling by helping to downregulate ribosomal gene expression when cells are exposed to hydrogen peroxide. An active actin cytoskeleton is required fo...

journal_title：FEMS yeast research

authors： Mitjana FV,Petkova MI,Pujol-Carrion N,de la Torre-Ruiz MA

更新日期：2011-12-01 00:00:00

abstract：:Using modern peptide analytical MS technology ('Peptidomics'), it is possible to analyze yeast α-pheromone both qualitatively and semi-quantitatively directly from conditioned cell culture media. MS/MS analysis shows both forms of α-pheromone (MFα and MFα') detectable and identifiable straight from WT supernatants. In...

journal_title：FEMS yeast research

authors： Bener Aksam E,Pinkse MW,Verhaert PD

更新日期：2013-05-01 00:00:00

abstract：:The roles of intracellular GTPase Arl1 and organellar cation/H(+) antiporters (Kha1 and Nhx1) in Saccharomyces cerevisiae tolerance to various stress factors were investigated and interesting new phenotypes of strains devoid of these proteins were found. The role of Arl1 GTPase in their tolerance to various cations is...

journal_title：FEMS yeast research

authors： Marešová L,Sychrová H

更新日期：2010-11-01 00:00:00

abstract：:The progenitor of the Dekkera/Brettanomyces clade separated from the Saccharomyces/Kluyveromyces clade over 200 million years ago. However, within both clades, several lineages developed similar physiological traits. Both Saccharomyces cerevisiae and Dekkera bruxellensis are facultative anaerobes; in the presence of e...

journal_title：FEMS yeast research

authors： Procházka E,Poláková S,Piskur J,Sulo P

更新日期：2010-08-01 00:00:00

abstract：:In this work, we show that the proteins Pkc1 and Pfy1 play a role in the repolarization of the actin cytoskeleton and in cell survival in response to oxidative stress. We have also developed an assay to determine the actin polymerization capacity of total protein extracts using fluorescence recovery after photobleachi...

journal_title：FEMS yeast research

authors： Pujol N,Bonet C,Vilella F,Petkova MI,Mozo-Villarías A,de la Torre-Ruiz MA

更新日期：2009-12-01 00:00:00

abstract：:The Tapirapé people of the Tapi'itãwa tribe of Brazil produce several fermented foods and beverages, one of which is called 'cauim'. This beverage usually makes up the main staple food for adults and children. Several substrates are used in its production, including cassava, rice, corn, maize and peanuts. A fermentati...

journal_title：FEMS yeast research

authors： Schwan RF,Almeida EG,Souza-Dias MA,Jespersen L

更新日期：2007-09-01 00:00:00

abstract：:Ume6p is a nonessential transcription factor that represses meiotic gene expression during vegetative growth in budding yeast. To relieve this repression, Ume6p is destroyed as cells enter meiosis and is not resynthesized until spore wall assembly. The present study reveals that spores derived from a ume6 null homozyg...

journal_title：FEMS yeast research

authors： Strich R,Khakhina S,Mallory MJ

更新日期：2011-02-01 00:00:00

abstract：:Wild-type strains of the thermotolerant methylotrophic yeast Hansenula polymorpha are able to ferment glucose, cellobiose and xylose to ethanol. H. polymorpha most actively fermented sugars to ethanol at 37 degrees C, whereas the well-known xylose-fermenting yeast Pichia stipitis could not effectively ferment carbon s...

journal_title：FEMS yeast research

authors： Ryabova OB,Chmil OM,Sibirny AA

更新日期：2003-11-01 00:00:00

abstract：:Coenzyme Q is a redox-active lipid that functions as an electron carrier in the mitochondrial respiratory chain. Q-biosynthesis in Saccharomyces cerevisiae requires at least nine proteins (Coq1p-Coq9p). The molecular function of Coq8p is still unknown; however, lack of Q and the concomitant accumulation of the interme...

journal_title：FEMS yeast research

authors： Tauche A,Krause-Buchholz U,Rödel G

更新日期：2008-12-01 00:00:00

abstract：:Schizosaccharomyces pombe Cap1 has been identified as the (adenylyl) cyclase-associated protein. Cap1 was able to bind Cap1 itself and actin. Cap1 localized at the growing tip, and this localization was dependent on the Cap1 P2 region. In a two-hybrid screening using cap1 as bait, we isolated csh3, which encodes a pro...

journal_title：FEMS yeast research

authors： Yamamoto T,Kobayashi-Ooka Y,Zhou GL,Kawamukai M

更新日期：2015-12-01 00:00:00

abstract：:Na,K-ATPase is a crucial enzyme for ion homeostasis in human tissues. Different isozymes are produced by assembly of four alpha- and three beta-subunits. The expression of the alpha3/beta1 isozyme is confined to brain and heart. Its heterologous production has so far never been attempted in a lower eukaryote. In this ...

journal_title：FEMS yeast research

authors： Reina C,Padoani G,Carotti C,Merico A,Tripodi G,Ferrari P,Popolo L

更新日期：2007-06-01 00:00:00

abstract：:We studied the effect of the loss of the Ser-Thr protein phosphatase Sit4, an important post-translational regulator, on the steady-state levels of the low-affinity glucose transporter Hxt1p and observed a delay in its appearance after high glucose induction, slow growth, and diminished glucose consumption. By analyzi...

journal_title：FEMS yeast research

authors： Souza AA,Miranda MN,da Silva SF,Bozaquel-Morais B,Masuda CA,Ghislain M,Montero-Lomelí M

更新日期：2012-12-01 00:00:00

abstract：:In the macroscopic world, ecological interactions between multiple species of fauna and flora are recognised as major role-players in the evolution of any particular species. By comparison, research on ecological interactions as a driver of evolutionary adaptation in microbial ecosystems has been neglected. The evolut...

journal_title：FEMS yeast research

authors： Conacher CG,Rossouw D,Bauer FFB

更新日期：2019-11-01 00:00:00

abstract：:Zinc is a crucial mineral for all organisms as it is an essential cofactor for the proper function of a plethora of proteins and depletion of zinc causes oxidative stress. Glutathione is the major redox buffering agent in the cell and therefore important for mitigation of the adverse effects of oxidative stress. In ma...

journal_title：FEMS yeast research

authors： Steiger MG,Patzschke A,Holz C,Lang C,Causon T,Hann S,Mattanovich D,Sauer M

更新日期：2017-06-01 00:00:00

abstract：:The yeast Pichia pastoris is a widely used host organism for heterologous protein production. One of the basic steps for strain improvement is to ensure a sufficient level of transcription of the heterologous gene, based on promoter strength and gene copy number. To date, high-copy-number integrants of P. pastoris are...

journal_title：FEMS yeast research

authors： Marx H,Mecklenbräuker A,Gasser B,Sauer M,Mattanovich D

更新日期：2009-12-01 00:00:00

abstract：:The histone acetyltransferase Sas2 is part of the SAS-I complex and acetylates lysine 16 of histone H4 (H4 K16Ac) in the genome of Saccharomyces cerevisiae. Sas2-mediated H4 K16Ac is strongest over the coding region of genes with low expression. However, it is unclear how Sas2-mediated acetylation is incorporated into...

journal_title：FEMS yeast research

authors： Reiter C,Heise F,Chung HR,Ehrenhofer-Murray AE

更新日期：2015-11-01 00:00:00

abstract：:We have cloned the Hansenula polymorpha SWI1 and SNF2 genes by functional complementation of mutants that are defective in methanol utilisation. These genes encode proteins similar to Saccharomyces cerevisiae Swi1p and Snf2p, which are subunits of the SWI/SNF complex. This complex belongs to the family of nucleosome-r...

journal_title：FEMS yeast research

authors： Ozimek P,Lahtchev K,Kiel JA,Veenhuis M,van der Klei IJ

更新日期：2004-05-01 00:00:00

abstract：:The FLO11-encoded flocculin is required for a variety of important phenotypes in Saccharomyces cerevisiae, including flocculation, adhesion to agar and plastic, invasive growth, pseudohyphae formation and biofilm development. We present evidence that Flo11p belongs to the Flo1-type class of flocculins rather than to t...

journal_title：FEMS yeast research

authors： Bayly JC,Douglas LM,Pretorius IS,Bauer FF,Dranginis AM

更新日期：2005-12-01 00:00:00

abstract：:The closely related yeasts Debaryomyces fabryi and Debaryomyces hansenii are excellent xylose consumers. We previously described the activity of a high-affinity xylose/H(+) symport from an industrial strain of D. hansenii subsequently reclassified as D. fabryi. We now report the identification of the gene encoding thi...

journal_title：FEMS yeast research

authors： Ferreira D,Nobre A,Silva ML,Faria-Oliveira F,Tulha J,Ferreira C,Lucas C

更新日期：2013-11-01 00:00:00

abstract：:Brettanomyces bruxellensis is usually considered a spoilage microorganism, responsible for significant economic losses during the production of fermented beverages such as wine, beer and cider, though for some styles of beer its influence is essential. In recent years, the competitiveness of this yeast in bioethanol p...

journal_title：FEMS yeast research

authors： Varela C,Lleixà J,Curtin C,Borneman A

更新日期：2018-11-01 00:00:00

abstract：:The mechanistic details of mtDNA maintenance in petite-negative yeasts have remained largely unexplored. We report here that the DNA helicase Hmi1p plays a crucial role in mtDNA stability in Candida albicans. Like its counterpart in Saccharomyces cerevisiae, Hmi1p in C. albicans (CaHmi1p) contains a C-terminal mitocho...

journal_title：FEMS yeast research

authors： Jõers P,Gerhold JM,Sedman T,Kuusk S,Sedman J

更新日期：2007-01-01 00:00:00

abstract：:High-level production of bioethanol (140 g L(-1) in 45 h) in aerated fed-batch cultures of Saccharomyces cerevisiae was shown to be linked to the length of a production phase uncoupled to the growth. The induction of this phase was characterized by metabolic and morphologic changes reminiscent of those occurring in th...

journal_title：FEMS yeast research

authors： Benbadis L,Cot M,Rigoulet M,Francois J

更新日期：2009-12-01 00:00:00

abstract：:Current paradigms assume that gas bubbles cannot be formed within yeasts although these workhorses of the baking and brewing industries vigorously produce and release CO(2) gas. We show that yeasts produce gas bubbles that fill a significant part of the cell. The missing link between intracellular CO(2) production b...

journal_title：FEMS yeast research

authors： Swart CW,Dithebe K,Pohl CH,Swart HC,Coetsee E,van Wyk PW,Swarts JC,Lodolo EJ,Kock JL

更新日期：2012-11-01 00:00:00

abstract：:Selenium (Se) is an essential element for most eukaryotic organisms, including humans. The balance between Se toxicity and its beneficial effects is very delicate. It has been demonstrated that a diet enriched with Se has cancer prevention potential in humans. The most popular commercial Se supplementation is selenize...

journal_title：FEMS yeast research

authors： Mapelli V,Hillestrøm PR,Patil K,Larsen EH,Olsson L

更新日期：2012-02-01 00:00:00

abstract：:Candida albicans is an important opportunistic yeast pathogen of humans and has the ability to form drug-resistant biofilms, with increased expression of multidrug ATP-binding cassette (ABC) transporters. These biofilms are also capable of secreting immune-modulating prostaglandin E2 (PGE2 ) from host-derived arachido...

journal_title：FEMS yeast research

authors： Ells R,Kemp G,Albertyn J,Kock JL,Pohl CH

更新日期：2013-12-01 00:00:00

abstract：:Saccharomyces cerevisiae yeast strains exhibit a huge genotypic and phenotypic diversity. Breeding strategies taking advantage of these characteristics would contribute greatly to improving industrial yeasts. Here we mapped and introgressed chromosomal regions controlling industrial yeast properties, such as hydrogen ...

journal_title：FEMS yeast research

authors： Marullo P,Yvert G,Bely M,Aigle M,Dubourdieu D

更新日期：2007-12-01 00:00:00

abstract：:Saccharomyces cerevisiae is the most widely used fermentation organism for ethanol production. However, the gene expression regulatory networks behind the ethanol fermentation are still not fully understood. Using a static fermentation model, we examined the ethanol yields on biomass of deletion mutants for 77 yeast g...

journal_title：FEMS yeast research

authors： Wu Y,Du J,Xu G,Jiang L

更新日期：2016-05-01 00:00:00

abstract：:A new, heat shock-inducible expression system based on an endogenous hsp16+ promoter was developed for use in the fission yeast Schizosaccharomyces pombe. Analysis of GFP expression profiles indicated that a 1.2-kb segment of the hsp16+ promoter region was sufficient to drive expression of heterologous protein. The hs...

journal_title：FEMS yeast research

authors： Fujita Y,Tohda H,Giga-Hama Y,Takegawa K

更新日期：2006-09-01 00:00:00

abstract：:Proteostasis is a cellular housekeeping process that refers to the healthy maintenance of the cellular proteome that governs the fate of proteins from synthesis to degradation. Perturbations of proteostasis might result in protein dysfunction with consequent deleterious effects that can culminate in cell death. To dea...

journal_title：FEMS yeast research

authors： Sampaio-Marques B,Ludovico P

更新日期：2018-08-01 00:00:00

abstract：:Yarrowia lipolytica is a dimorphic fungus whose morphology is controlled by several factors such as pH and different compounds. To determine if the STE11-mitogen-activated protein kinase (MAPK) pathway plays a role in dimorphism of Y. lipolytica, we isolated the gene encoding a Mapkkk. The isolated gene (STE11) has an...

journal_title：FEMS yeast research

authors： Cervantes-Chávez JA,Ruiz-Herrera J

更新日期：2006-08-01 00:00:00