Development of a genetic transformation toolkit for Brettanomyces bruxellensis.

abstract：:The LYS12 gene from Candida albicans, coding for homoisocitrate dehydrogenase was cloned and expressed as a His-tagged protein in Escherichia coli. The purified gene product catalyzes the Mg(2+)- and K(+)-dependent oxidative decarboxylation of homoisocitrate to α-ketoadipate. The recombinant enzyme demonstrates strict...

journal_title：FEMS yeast research

authors： Gabriel I,Vetter ND,Palmer DR,Milewska MJ,Wojciechowski M,Milewski S

更新日期：2013-03-01 00:00:00

abstract：:Proteostasis is a cellular housekeeping process that refers to the healthy maintenance of the cellular proteome that governs the fate of proteins from synthesis to degradation. Perturbations of proteostasis might result in protein dysfunction with consequent deleterious effects that can culminate in cell death. To dea...

journal_title：FEMS yeast research

authors： Sampaio-Marques B,Ludovico P

更新日期：2018-08-01 00:00:00

abstract：:Extracellular proteins of Cryptococcus neoformans are involved in the pathogenesis of cryptococcosis, and some are immunoreactive antigens that may potentially serve as candidates for vaccine development. To further study the extracellular proteome of the human fungal pathogen Cry. neoformans, we conducted a proteomic...

journal_title：FEMS yeast research

authors： Eigenheer RA,Jin Lee Y,Blumwald E,Phinney BS,Gelli A

更新日期：2007-06-01 00:00:00

abstract：:Candida glabrata is a second most common human opportunistic pathogen which causes superficial but also life-threatening systemic candidosis. According to the localisation of mannans and mannoproteins in the outermost layer of the cell wall, mannan detection could be one of the first steps in the cell recognition of C...

journal_title：FEMS yeast research

authors： Paulovičová L,Paulovičová E,Farkaš P,Čížová A,Bystrický P,Jančinová V,Turánek J,Pericolini E,Gabrielli E,Vecchiarelli A,Hrubiško M

更新日期：2019-03-01 00:00:00

abstract：:Secondary metabolites are an important source of high-value chemicals, many of which exhibit important pharmacological properties. These valuable natural products are often difficult to synthesize chemically and are commonly isolated through inefficient extractions from natural biological sources. As such, they are in...

journal_title：FEMS yeast research

authors： Siddiqui MS,Thodey K,Trenchard I,Smolke CD

更新日期：2012-03-01 00:00:00

abstract：:Fungal wall formation is a dynamic process involving several categories of enzymes. The GH72 family of β(1,3)-glucanosyltransferases is essential for the determination of cell shape, for cell integrity and for virulence in pathogenic fungi. Candida albicans has five GH72 genes: PHR1 and PHR2 are pH dependent, the firs...

journal_title：FEMS yeast research

authors： Kováčová K,Degani G,Stratilová E,Farkaš V,Popolo L

更新日期：2015-03-01 00:00:00

abstract：:A biofilm-forming strain of Pichia fermentans proved to be most effective in controlling brown rot on apple fruit when coinoculated into artificial wounds with a phytopathogenic isolate of Monilinia fructicola. Culture filtrates and autoclaved cells had no significant influence on the disease. When sprayed onto the ap...

journal_title：FEMS yeast research

authors： Giobbe S,Marceddu S,Scherm B,Zara G,Mazzarello VL,Budroni M,Migheli Q

更新日期：2007-12-01 00:00:00

abstract：:Candida lipolytica and Candida rugosa were isolated from blood samples from a patient with chronic myeloid leukemia (31 years old) and a patient with sickle cell disease (1-year-old), respectively. Isolates were grown for 48 h at 37 degrees C in either Sabouraud or tryptone soy broth (TSB). Peptidases were tested for ...

journal_title：FEMS yeast research

authors： De Melo AC,Dornelas-Ribeiro M,De Souza EP,Macrae A,Fracalanzza SE,Vermelho AB

更新日期：2007-09-01 00:00:00

abstract：:The essentiality of iodine for humans, especially in the early stages of life, is well recognized. The chemical forms of iodine in food supplements, infant formulae and iodated salt are either iodide (KI) or iodate (KIO(3)). Because there are no or rare data about iodine uptake by yeasts, we investigated the influence...

journal_title：FEMS yeast research

authors： Osterc A,Fujs S,Raspor P,Stibilj V

更新日期：2009-02-01 00:00:00

abstract：:Many of the difficulties of studying evolution in action can be surmounted using populations of microorganisms, such as yeast. A readily manipulated sexual system and an increasingly sophisticated array of molecular and genomic tools uniquely qualify Saccharomyces cerevisiae as an experimental subject. This minireview...

journal_title：FEMS yeast research

authors： Zeyl C

更新日期：2006-08-01 00:00:00

abstract：:Mitogen activated protein kinase (MAPK) cascades are signal transduction mechanisms present in eukaryotic cells that allow adaptation to environmental changes. MAPK activity is mainly regulated by dual phosphorylation in a TXY motif present in the kinase subdomain VIII as well as dephosphorylation by specific phosphat...

journal_title：FEMS yeast research

authors： Román E,Alonso-Monge R,Gong Q,Li D,Calderone R,Pla J

更新日期：2009-09-01 00:00:00

abstract：:The microbial consortium of wine fermentations is highly dependent upon winemaking decisions made at crush, including the decision to inoculate and the decision to add sulfur dioxide (SO2) to the must. To investigate this, Chardonnay grape juice was subjected to two inoculation treatments (uninoculated and pied de cuv...

journal_title：FEMS yeast research

authors： Morgan SC,McCarthy GC,Watters BS,Tantikachornkiat M,Zigg I,Cliff MA,Durall DM

更新日期：2019-08-01 00:00:00

abstract：:Lignocellulosic plant biomass is the target feedstock for production of second-generation biofuels. Ionic liquid (IL) pretreatment can enhance deconstruction of lignocellulosic biomass into sugars that can be fermented to ethanol. Although biomass is typically washed following IL pretreatment, small quantities of resi...

journal_title：FEMS yeast research

authors： Sitepu IR,Shi S,Simmons BA,Singer SW,Boundy-Mills K,Simmons CW

更新日期：2014-12-01 00:00:00

abstract：:Non-Saccharomyces yeasts impact wine fermentations and can diversify the flavor profiles of wines. However, little information is available on the metabolic networks of most of these species. Here we show that unlike the main wine yeast Saccharomyces cerevisiae, Torulaspora delbrueckii and to a lesser extent Lachancea...

journal_title：FEMS yeast research

authors： Mbuyane LL,de Kock M,Bauer FF,Divol B

更新日期：2018-11-01 00:00:00

abstract：:We used multi-locus sequence typing (MLST) to investigate 35 yeast isolates representing the two genome-sequenced strains plus the type strain of Candida albicans, four isolates originally identified as Candida stellatoidea type I and 28 representing type strains of other species now regarded as synonymous with C. alb...

journal_title：FEMS yeast research

authors： Jacobsen MD,Boekhout T,Odds FC

更新日期：2008-08-01 00:00:00

abstract：:In the methylotrophic yeast Hansenula polymorpha non-selective autophagy, induced by nitrogen starvation, results in the turnover of cytoplasmic components, including peroxisomes. We show that the uptake of these components occurs by invagination of the vacuolar membrane without their prior sequestration and thus diff...

journal_title：FEMS yeast research

authors： Bellu AR,Kram AM,Kiel JA,Veenhuis M,van der Klei IJ

更新日期：2001-04-01 00:00:00

abstract：:Candida albicans maintains genetic diversity by random chromosome alterations, and this diversity allows utilization of various nutrients. Although the alterations seem to occur spontaneously, their frequencies clearly depend on environmental factors. In addition, this microorganism survives in adverse environments, w...

journal_title：FEMS yeast research

authors： Rustchenko E

更新日期：2007-01-01 00:00:00

abstract：:The GPD1 gene encoding the glycerol-3-phosphate dehydrogenase was overexpressed in an industrial lager brewing yeast (Saccharomyces cerevisiae ssp. carlsbergensis) to reduce the content of ethanol in beer. The amount of glycerol produced by the GPD1-overexpressing yeast in fermentation experiments simulating brewing c...

journal_title：FEMS yeast research

authors： Nevoigt E,Pilger R,Mast-Gerlach E,Schmidt U,Freihammer S,Eschenbrenner M,Garbe L,Stahl U

更新日期：2002-05-01 00:00:00

abstract：:Nine yeast strains were isolated from spontaneous fermentations in the Alsace area of France, during the 1997, 1998 and 1999 grape harvests. Strains were characterized by pulsed-field gel electrophoresis, PCR-restriction fragment length polymorphism (RFLP) of the MET2 gene, delta-PCR, and microsatellite patterns. Kary...

journal_title：FEMS yeast research

authors： Le Jeune C,Lollier M,Demuyter C,Erny C,Legras JL,Aigle M,Masneuf-Pomarède I

更新日期：2007-06-01 00:00:00

abstract：:In the pathogenic fungus Candida glabrata, the YPS1 gene, which encodes a glycosylphosphatidylinositol-linked aspartyl protease, is required for cell wall integrity and virulence. Although the expression of YPS1 has been studied in Saccharomyces cerevisiae, the transcriptional regulation of this gene in C. glabrata is...

journal_title：FEMS yeast research

authors： Miyazaki T,Izumikawa K,Yamauchi S,Inamine T,Nagayoshi Y,Saijo T,Seki M,Kakeya H,Yamamoto Y,Yanagihara K,Miyazaki Y,Yasuoka A,Kohno S

更新日期：2011-08-01 00:00:00

abstract：:Five yeast isolates obtained from cashew tree flowers in Nigeria resembled Cryptococcus neoformans phenotypically by producing brown pigmented colonies on niger seed agar, expressing a capsule, and being able to grow at 37 degrees C. However, rRNA gene sequences, including the 18S rRNA gene, the D1/D2 domains of the 2...

journal_title：FEMS yeast research

authors： Okoli I,Oyeka CA,Kwon-Chung KJ,Theelen B,Robert V,Groenewald JZ,McFadden DC,Casadevall A,Boekhout T

更新日期：2007-03-01 00:00:00

abstract：:Ume6p is a nonessential transcription factor that represses meiotic gene expression during vegetative growth in budding yeast. To relieve this repression, Ume6p is destroyed as cells enter meiosis and is not resynthesized until spore wall assembly. The present study reveals that spores derived from a ume6 null homozyg...

journal_title：FEMS yeast research

authors： Strich R,Khakhina S,Mallory MJ

更新日期：2011-02-01 00:00:00

abstract：:Saccharomyces cerevisiae grown on plastic surfaces formed organized structures, termed minicolonies, that consisted of a core of round (yeast-like) cells surrounded by chains of filamentous cells (pseudohyphae). Minicolonies had a much higher affinity for plastic than unstructured yeast communities growing on the same...

journal_title：FEMS yeast research

authors： White MG,Piccirillo S,Dusevich V,Law DJ,Kapros T,Honigberg SM

更新日期：2011-03-01 00:00:00

abstract：:Previous work has shown that the synthetic lethality of the slt2Δrim101Δ mutant results from a combination of factors, including improper functioning of the septum assembly machinery. Here, we identify new multicopy suppressors of this lethality including Kss1, Pcl1 and Sph1, none of which seems to be linked to the up...

journal_title：FEMS yeast research

authors： Pérez J,Arcones I,Gómez A,Casquero V,Roncero C

更新日期：2016-09-01 00:00:00

abstract：:In the macroscopic world, ecological interactions between multiple species of fauna and flora are recognised as major role-players in the evolution of any particular species. By comparison, research on ecological interactions as a driver of evolutionary adaptation in microbial ecosystems has been neglected. The evolut...

journal_title：FEMS yeast research

authors： Conacher CG,Rossouw D,Bauer FFB

更新日期：2019-11-01 00:00:00

abstract：:The yeast Pichia pastoris is a widely used host organism for heterologous protein production. One of the basic steps for strain improvement is to ensure a sufficient level of transcription of the heterologous gene, based on promoter strength and gene copy number. To date, high-copy-number integrants of P. pastoris are...

journal_title：FEMS yeast research

authors： Marx H,Mecklenbräuker A,Gasser B,Sauer M,Mattanovich D

更新日期：2009-12-01 00:00:00

abstract：:First-generation (1G) fuel ethanol production in sugarcane-based biorefineries is an established economic enterprise in Brazil. Second-generation (2G) fuel ethanol from lignocellulosic materials, though extensively investigated, is currently facing severe difficulties to become economically viable. Some of the challen...

journal_title：FEMS yeast research

authors： Bermejo PM,Raghavendran V,Gombert AK

更新日期：2020-06-01 00:00:00

abstract：:Homologous recombination (HR) is an error-free DNA repair mechanism that maintains genome integrity by repairing double-strand breaks (DSBs). Defects in HR lead to genomic instability and are associated with cancer predisposition. A key step in HR is the formation of Rad51 nucleoprotein filaments which are responsible...

journal_title：FEMS yeast research

authors： Martino J,Bernstein KA

更新日期：2016-09-01 00:00:00

abstract：:The mechanistic details of mtDNA maintenance in petite-negative yeasts have remained largely unexplored. We report here that the DNA helicase Hmi1p plays a crucial role in mtDNA stability in Candida albicans. Like its counterpart in Saccharomyces cerevisiae, Hmi1p in C. albicans (CaHmi1p) contains a C-terminal mitocho...

journal_title：FEMS yeast research

authors： Jõers P,Gerhold JM,Sedman T,Kuusk S,Sedman J

更新日期：2007-01-01 00:00:00

abstract：:Promoter of alcohol oxidase I (PAOX1) is the most efficient promoter involved in the regulation of recombinant protein expression in Pichia pastoris (P. pastoris). PAOX1 is tightly repressed by the presence of glycerol in the culture medium; thus, glycerol must be exhausted before methanol can be taken up by P. pastor...

journal_title：FEMS yeast research

authors： Zhan C,Wang S,Sun Y,Dai X,Liu X,Harvey L,McNeil B,Yang Y,Bai Z

更新日期：2016-06-01 00:00:00