Linking cellular proteostasis to yeast longevity.

abstract：:Screening for genes affecting super-secreting phenotype of the over-secreting mutant of Kluyveromyces lactis resulted in isolation of the gene named KlMNN10, sharing high homology with Saccharomyces cerevisiae MNN10. The disruption of the KlMNN10 in Kluyveromyces lactis, as well as of MNN10 and MNN11 in Saccharomyces ...

journal_title：FEMS yeast research

authors： Bartkeviciūte D,Sasnauskas K

更新日期：2004-09-01 00:00:00

abstract：:Lignocellulosic plant biomass is the target feedstock for production of second-generation biofuels. Ionic liquid (IL) pretreatment can enhance deconstruction of lignocellulosic biomass into sugars that can be fermented to ethanol. Although biomass is typically washed following IL pretreatment, small quantities of resi...

journal_title：FEMS yeast research

authors： Sitepu IR,Shi S,Simmons BA,Singer SW,Boundy-Mills K,Simmons CW

更新日期：2014-12-01 00:00:00

abstract：:The biosynthesis of yeast phosphatidylglycerol (PG) takes place in the inner mitochondrial membrane. Outside mitochondria, the abundance of PG is low. Here, we present evidence that the subcellular distribution of PG is maintained by the locally controlled enzymatic activity of the PG-specific phospholipase, Pgc1. A f...

journal_title：FEMS yeast research

authors： Kubalová D,Káňovičová P,Veselá P,Awadová T,Džugasová V,Daum G,Malínský J,Balážová M

更新日期：2019-08-01 00:00:00

abstract：:Candida lipolytica and Candida rugosa were isolated from blood samples from a patient with chronic myeloid leukemia (31 years old) and a patient with sickle cell disease (1-year-old), respectively. Isolates were grown for 48 h at 37 degrees C in either Sabouraud or tryptone soy broth (TSB). Peptidases were tested for ...

journal_title：FEMS yeast research

authors： De Melo AC,Dornelas-Ribeiro M,De Souza EP,Macrae A,Fracalanzza SE,Vermelho AB

更新日期：2007-09-01 00:00:00

abstract：:Although exposure of Candida albicans cells to high-dose (4 mM) methylprednisolone stimulated microbial growth, germination rate in serum and phospholipase release, it also promoted the recognition of C. albicans cells by polymorphonuclear leukocytes. Pretreatment of C. albicans cells with methylprednisolone did not r...

journal_title：FEMS yeast research

authors： Gyetvai A,Emri T,Fekete A,Varga Z,Gazdag Z,Pesti M,Belágyi J,Emõdy L,Pócsi I,Lenkey B

更新日期：2007-03-01 00:00:00

abstract：:Eight yeast strains that asexually reproduce by cell fission were isolated from bee bread of different solitary bees in Germany. DNA sequence analysis revealed that the strains shared the same sequence in the D1/D2 domain of the nuclear large subunit (LSU) rRNA gene with a strain that was previously isolated from a fi...

journal_title：FEMS yeast research

authors： Brysch-Herzberg M,Tobias A,Seidel M,Wittmann R,Wohlmann E,Fischer R,Dlauchy D,Peter G

更新日期：2019-06-01 00:00:00

abstract：:The catabolism of methionine to methionol and methanethiol in Saccharomyces cerevisiae was studied using (13)C NMR spectroscopy, GC-MS, enzyme assays and a number of mutants. Methionine is first transaminated to alpha-keto-gamma-(methylthio)butyrate. Methionol is formed by a decarboxylation reaction, which yields meth...

journal_title：FEMS yeast research

authors： Perpète P,Duthoit O,De Maeyer S,Imray L,Lawton AI,Stavropoulos KE,Gitonga VW,Hewlins MJ,Dickinson JR

更新日期：2006-01-01 00:00:00

abstract：:We have reported previously that growth on alcohol vapors confers hemolytic properties on certain yeast species and strains ['microbial alcohol-conferred hemolysis' (MACH)]. In a recent study, we analyzed the genetic basis of MACH in Saccharomyces cerevisiae using the EUROSCARF mutant collection. The data suggested th...

journal_title：FEMS yeast research

authors： Shuster A,Korem M,Jacob-Hirsch J,Amariglio N,Rechavi G,Rosenberg M

更新日期：2011-06-01 00:00:00

abstract：:In previous communications the essential role of spermidine in Ustilago maydis was demonstrated by means of the disruption of the genes encoding ornithine decarboxylase (ODC) and spermidine synthase (SPE). However, the assignation of specific roles to each polyamine in different cellular functions was not possible bec...

journal_title：FEMS yeast research

authors： Valdés-Santiago L,Guzmán-de-Peña D,Ruiz-Herrera J

更新日期：2010-11-01 00:00:00

abstract：:The fungal cell wall is a coherent structure formed by microfibrillar polysaccharides and amorphous material made of other polysaccharides and proteins. We performed a phylogenetic analysis of covalent proteins and enzymes that synthesize fungal wall polysaccharides to determine the possible evolution of the wall stru...

journal_title：FEMS yeast research

authors： Ruiz-Herrera J,Ortiz-Castellanos L

更新日期：2010-05-01 00:00:00

abstract：:Current paradigms assume that gas bubbles cannot be formed within yeasts although these workhorses of the baking and brewing industries vigorously produce and release CO(2) gas. We show that yeasts produce gas bubbles that fill a significant part of the cell. The missing link between intracellular CO(2) production b...

journal_title：FEMS yeast research

authors： Swart CW,Dithebe K,Pohl CH,Swart HC,Coetsee E,van Wyk PW,Swarts JC,Lodolo EJ,Kock JL

更新日期：2012-11-01 00:00:00

abstract：:The LYS12 gene from Candida albicans, coding for homoisocitrate dehydrogenase was cloned and expressed as a His-tagged protein in Escherichia coli. The purified gene product catalyzes the Mg(2+)- and K(+)-dependent oxidative decarboxylation of homoisocitrate to α-ketoadipate. The recombinant enzyme demonstrates strict...

journal_title：FEMS yeast research

authors： Gabriel I,Vetter ND,Palmer DR,Milewska MJ,Wojciechowski M,Milewski S

更新日期：2013-03-01 00:00:00

abstract：:Zero-trans rates of maltose transport by brewer's yeasts exert strong control over fermentation rates and are strongly temperature-dependent over the temperature range (20–0 °C) of brewery fermentations. Three α-glucoside transporters, ScAgt1(A60) (a Saccharomyces cerevisiae version of Agt1 from an ale strain), ScAgt1...

journal_title：FEMS yeast research

authors： Vidgren V,Viljanen K,Mattinen L,Rautio J,Londesborough J

更新日期：2014-06-01 00:00:00

abstract：:CLC-type chloride/proton antiporters are required for copper/iron homeostasis in fungi. A relationship between CLCs and Ca(2+) homeostasis has not been found before. Here we demonstrate the requirement of the antiporter CLC1 for Ca(2+) homeostasis/signaling in Cryptococcus neoformans. The deletion of CLC1 in JEC21 r...

journal_title：FEMS yeast research

authors： Li D,Zhang X,Li Z,Yang J,Pan J,Zhu X

更新日期：2012-02-01 00:00:00

abstract：:The budding yeast, Saccharomyces cerevisiae, is an efficient model for studying oxidative stress, programmed cell death and aging. The present study was carried out to investigate antioxidant, the anti-apoptotic and anti-aging activity of a natural compound, astaxanthin, in S. cerevisiae model. The survivability of ye...

journal_title：FEMS yeast research

authors： Sj S,Veerabhadrappa B,Subramaniyan S,Dyavaiah M

更新日期：2019-01-01 00:00:00

abstract：:The fungus Paracoccidioides brasiliensis causes paracoccidioidomycosis, a systemic granulomatous mycosis prevalent in Latin America. In an effort to elucidate the molecular mechanisms involved in fungus cell wall assembly and morphogenesis, beta-1,3-glucanosyltransferase 3 (PbGel3p) is presented here. PbGel3p presente...

journal_title：FEMS yeast research

authors： Castro Nda S,de Castro KP,Orlandi I,Feitosa Ldos S,Rosa e Silva LK,Vainstein MH,Báo SN,Vai M,Soares CM

更新日期：2009-02-01 00:00:00

abstract：:This study addresses the question of whether observable changes in fluxes in the primary carbon metabolism of Saccharomyces cerevisiae occur between the different phases of the cell division cycle. To detect such changes by metabolic flux analysis, a 13C-labeling experiment was performed with a fed-batch culture inocu...

journal_title：FEMS yeast research

authors： Costenoble R,Müller D,Barl T,van Gulik WM,van Winden WA,Reuss M,Heijnen JJ

更新日期：2007-06-01 00:00:00

abstract：:Schizosaccharomyces pombe Cap1 has been identified as the (adenylyl) cyclase-associated protein. Cap1 was able to bind Cap1 itself and actin. Cap1 localized at the growing tip, and this localization was dependent on the Cap1 P2 region. In a two-hybrid screening using cap1 as bait, we isolated csh3, which encodes a pro...

journal_title：FEMS yeast research

authors： Yamamoto T,Kobayashi-Ooka Y,Zhou GL,Kawamukai M

更新日期：2015-12-01 00:00:00

abstract：:There is increasing evidence in the literature showing that fungal pathogens express biologically active ectoenzymes. The expression of surface phosphatases at the cell surface of Cryptococcus neoformans, the etiologic agent of cryptococcosis, was evaluated in the present study. Different isolates of C. neoformans exp...

journal_title：FEMS yeast research

authors： Collopy-Junior I,Esteves FF,Nimrichter L,Rodrigues ML,Alviano CS,Meyer-Fernandes JR

更新日期：2006-11-01 00:00:00

abstract：:The target of rapamycin complex 1 (TORC1) is the central controller of growth in eukaryotic cells. As one of the downstream targets of TORC1, the protein kinase ScSch9p plays multiple roles in stress resistance, longevity and nutrient sensing in Saccharomyces cerevisiae. In this study, we demonstrate that Candida albi...

journal_title：FEMS yeast research

authors： Liu W,Zhao J,Li X,Li Y,Jiang L

更新日期：2010-06-01 00:00:00

abstract：:In yeast, rRNA genes can be detected with the FISH technique using rRNA gene probes. This technique yields reliable, reproducible and precise results, but is time-consuming. Here, the primed in situ DNA synthesis (PRINS) procedure has been optimized for rapid detection of yeast rRNA genes. PRINS, which is as sensitive...

journal_title：FEMS yeast research

authors： Wnuk M,Lewinska A,Bugno M,Bartosz G,Slota E

更新日期：2009-06-01 00:00:00

abstract：:The amplification by PCR of the Intergenic Spacer region (IGS) of rDNA followed by Restriction Fragment Length Polymorphism (RFLP) analysis was evaluated as a potential method for the identification of Debaryomyces hansenii among other yeast species that frequently contaminate Intermediate-Moisture Foods (IMFs). For a...

journal_title：FEMS yeast research

authors： Romero P,Patiño B,Quirós M,González-Jaén MT,Valderrama MJ,de Silóniz MI,Peinado JM

更新日期：2005-02-01 00:00:00

abstract：:Xylose fermentation is a rare trait that is immensely important to the cellulosic biofuel industry, and Candida tenuis is one of the few yeasts that has been reported with this trait. Here we report the isolation of two strains representing a candidate sister species to C. tenuis. Integrated analysis of genome sequenc...

journal_title：FEMS yeast research

authors： Haase MAB,Kominek J,Langdon QK,Kurtzman CP,Hittinger CT

更新日期：2017-05-01 00:00:00

abstract：:Loss of mtDNA by the petite-negative yeast Kluyveromyces lactis is lethal (rho(o)-lethality). However, mutations in the alpha, beta and gamma subunits of F(1)-ATPase can suppress lethality by increasing intramitochondrial hydrolysis of ATP. Increased hydrolysis of ATP can also occur on inactivation of Inh1, the natura...

journal_title：FEMS yeast research

authors： Clark-Walker GD

更新日期：2007-08-01 00:00:00

abstract：:Aldose-1-epimerase or mutarotase (EC 5.1.3.3) catalyzes interconversion of alpha/beta-anomers of aldoses, such as glucose and galactose, and is distributed in a wide variety of organisms from bacteria to humans. Nevertheless, the physiological role of this enzyme has been elusive in most cases, because the alpha-form ...

journal_title：FEMS yeast research

authors： Fukasawa T,Sakurai H,Nogi Y,Baruffini E

更新日期：2009-08-01 00:00:00

abstract：:Saccharomyces cerevisiae shows similar responses to heat shock and ethanol stress. Cells treated with severe ethanol stress activate the transcription of HSP genes and cause the aggregation of Hsp104-GFP, implying that severe ethanol stress as well as heat shock causes the accumulation of denatured proteins in yeast c...

journal_title：FEMS yeast research

authors： Kato S,Yoshida M,Izawa S

更新日期：2019-12-01 00:00:00

abstract：:Conversion of glucose to lactic acid is stoichiometrically equivalent to ethanol formation with respect to ATP formation from substrate-level phosphorylation, redox equivalents and product yield. However, anaerobic growth cannot be sustained in homolactate fermenting Saccharomyces cerevisiae. ATP-dependent export of t...

journal_title：FEMS yeast research

authors： Abbott DA,van den Brink J,Minneboo IM,Pronk JT,van Maris AJ

更新日期：2009-05-01 00:00:00

abstract：:Genetic relationships of 24 phenotypically different strains isolated from sorghum beer in West Africa and the type cultures of the Saccharomyces sensu stricto species were investigated by universally primed polymerase chain reaction (PCR) analysis, microsatellite fingerprinting and PCR-restriction fragment length pol...

journal_title：FEMS yeast research

authors： Naumova ES,Korshunova IV,Jespersen L,Naumov GI

更新日期：2003-04-01 00:00:00

abstract：:Cadmium ions disrupt reactive oxygen species/Ca(2+) homeostasis and subsequently elicit cell death and adaptive signaling cascades in eukaryotic cells. Through a functional genomics approach, we have identified deletion mutants of 106 yeast genes, including three MAP kinase genes (HOG1, SLT2, and KSS1), are sensitive ...

journal_title：FEMS yeast research

authors： Jiang L,Cao C,Zhang L,Lin W,Xia J,Xu H,Zhang Y

更新日期：2014-12-01 00:00:00

abstract：:Na,K-ATPase is a crucial enzyme for ion homeostasis in human tissues. Different isozymes are produced by assembly of four alpha- and three beta-subunits. The expression of the alpha3/beta1 isozyme is confined to brain and heart. Its heterologous production has so far never been attempted in a lower eukaryote. In this ...

journal_title：FEMS yeast research

authors： Reina C,Padoani G,Carotti C,Merico A,Tripodi G,Ferrari P,Popolo L

更新日期：2007-06-01 00:00:00