Abstract:
:The amplification by PCR of the Intergenic Spacer region (IGS) of rDNA followed by Restriction Fragment Length Polymorphism (RFLP) analysis was evaluated as a potential method for the identification of Debaryomyces hansenii among other yeast species that frequently contaminate Intermediate-Moisture Foods (IMFs). For a first rapid differentiation at the species level, the determination of the IGS-PCR fragment size was found to be a useful approach. The digestion of this region with the enzymes HhaI, HapII and MboI resulted in specific patterns that permit the identification of D. hansenii among other yeast species. This method also permitted the discrimination between the D. hansenii varieties (var. hansenii and var. fabryi) as well as the differentiation of D. hansenii from other species of the genus, such as Debaryomyces pseudopolymorphus or Debaryomyces polymorphus var. polymorphus. The IGS-PCR RFLP method was assayed for the differential detection of D. hansenii in contaminated or spoiled IMF products and compared with traditional identification procedures, resulting in a 100% detection rate for D. hansenii.
journal_name
FEMS Yeast Resjournal_title
FEMS yeast researchauthors
Romero P,Patiño B,Quirós M,González-Jaén MT,Valderrama MJ,de Silóniz MI,Peinado JMdoi
10.1016/j.femsyr.2004.09.002keywords:
subject
Has Abstractpub_date
2005-02-01 00:00:00pages
455-61issue
4-5eissn
1567-1356issn
1567-1364pii
S1567-1356(04)00136-9journal_volume
5pub_type
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