Schizosaccharomyces osmophilus sp. nov., an osmophilic fission yeast occurring in bee bread of different solitary bee species.

Abstract:

:Eight yeast strains that asexually reproduce by cell fission were isolated from bee bread of different solitary bees in Germany. DNA sequence analysis revealed that the strains shared the same sequence in the D1/D2 domain of the nuclear large subunit (LSU) rRNA gene with a strain that was previously isolated from a fig snack from Spain. The closest related type strain was that of Schizosaccharomyces octosporus, which showed 98.2% sequence similarity (11 substitutions) with the new strains. By clone sequence analysis of the internal transcribed spacer (ITS) region (ITS1, 5.8S rDNA, and ITS2) a total of nine different copy types were identified. The new strains differed from S. octosporus by approximately 31% in the ITS region. Sequence analysis of the RNAse P gene further supported the description of a new species. The strains isolated during this study show some phenotypic characteristics that separate them from the closest related species, S. octosporus and S. cryophilus. Since all strains showed true osmophily the name of the new species is S. osmophilus (holotype: CBS 15793T; isotype: CLIB 3267 T = NCAIM Y.02225 T, MycoBank no.: MB829586).

journal_name

FEMS Yeast Res

journal_title

FEMS yeast research

authors

Brysch-Herzberg M,Tobias A,Seidel M,Wittmann R,Wohlmann E,Fischer R,Dlauchy D,Peter G

doi

10.1093/femsyr/foz038

subject

Has Abstract

pub_date

2019-06-01 00:00:00

issue

4

eissn

1567-1356

issn

1567-1364

pii

5499025

journal_volume

19

pub_type

杂志文章
  • The heme-binding protein Dap1 links iron homeostasis to azole resistance via the P450 protein Erg11 in Candida glabrata.

    abstract::The pathogenic fungus Candida glabrata is relatively resistant to azole antifungals, which target lanosterol 14α-demethylase (Erg11p) in the ergosterol biosynthesis pathway. Our study revealed that C. glabrata exhibits increased azole susceptibility under low-iron conditions. To investigate the molecular basis of this...

    journal_title:FEMS yeast research

    pub_type: 杂志文章

    doi:10.1111/1567-1364.12043

    authors: Hosogaya N,Miyazaki T,Nagi M,Tanabe K,Minematsu A,Nagayoshi Y,Yamauchi S,Nakamura S,Imamura Y,Izumikawa K,Kakeya H,Yanagihara K,Miyazaki Y,Kugiyama K,Kohno S

    更新日期:2013-06-01 00:00:00

  • Neither 1G nor 2G fuel ethanol: setting the ground for a sugarcane-based biorefinery using an iSUCCELL yeast platform.

    abstract::First-generation (1G) fuel ethanol production in sugarcane-based biorefineries is an established economic enterprise in Brazil. Second-generation (2G) fuel ethanol from lignocellulosic materials, though extensively investigated, is currently facing severe difficulties to become economically viable. Some of the challen...

    journal_title:FEMS yeast research

    pub_type: 杂志文章

    doi:10.1093/femsyr/foaa027

    authors: Bermejo PM,Raghavendran V,Gombert AK

    更新日期:2020-06-01 00:00:00

  • Analysis of Saccharomyces cerevisiae hexose carrier expression during wine fermentation: both low- and high-affinity Hxt transporters are expressed.

    abstract::The transport of glucose and fructose into yeast cells is a critical step in the utilization of sugars during wine fermentation. Hexose uptake can be carried out by various Hxt carriers, each possessing distinct regulatory and transport-kinetic properties capable of influencing yeast fermentation capacity. We investig...

    journal_title:FEMS yeast research

    pub_type: 杂志文章

    doi:10.1016/j.femsyr.2004.09.005

    authors: Perez M,Luyten K,Michel R,Riou C,Blondin B

    更新日期:2005-02-01 00:00:00

  • The yeast Zygosaccharomyces bailii: a new host for heterologous protein production, secretion and for metabolic engineering applications.

    abstract::Molecular tools for the production of heterologous proteins and metabolic engineering applications of the non-conventional yeast Zygosaccharomyces bailii were developed. The combination of Z. bailii's resistance to relatively high temperature, osmotic pressure and low pH values, with a high specific growth rate render...

    journal_title:FEMS yeast research

    pub_type: 杂志文章

    doi:10.1016/S1567-1356(03)00200-9

    authors: Branduardi P,Valli M,Brambilla L,Sauer M,Alberghina L,Porro D

    更新日期:2004-01-01 00:00:00

  • Cadmium-induced activation of high osmolarity glycerol pathway through its Sln1 branch is dependent on the MAP kinase kinase kinase Ssk2, but not its paralog Ssk22, in budding yeast.

    abstract::Cadmium ions disrupt reactive oxygen species/Ca(2+) homeostasis and subsequently elicit cell death and adaptive signaling cascades in eukaryotic cells. Through a functional genomics approach, we have identified deletion mutants of 106 yeast genes, including three MAP kinase genes (HOG1, SLT2, and KSS1), are sensitive ...

    journal_title:FEMS yeast research

    pub_type: 杂志文章

    doi:10.1111/1567-1364.12220

    authors: Jiang L,Cao C,Zhang L,Lin W,Xia J,Xu H,Zhang Y

    更新日期:2014-12-01 00:00:00

  • Peptidase profiles from non-albicans Candida spp. isolated from the blood of a patient with chronic myeloid leukemia and another with sickle cell disease.

    abstract::Candida lipolytica and Candida rugosa were isolated from blood samples from a patient with chronic myeloid leukemia (31 years old) and a patient with sickle cell disease (1-year-old), respectively. Isolates were grown for 48 h at 37 degrees C in either Sabouraud or tryptone soy broth (TSB). Peptidases were tested for ...

    journal_title:FEMS yeast research

    pub_type: 杂志文章

    doi:10.1111/j.1567-1364.2007.00269.x

    authors: De Melo AC,Dornelas-Ribeiro M,De Souza EP,Macrae A,Fracalanzza SE,Vermelho AB

    更新日期:2007-09-01 00:00:00

  • Three Agt1 transporters from brewer's yeasts exhibit different temperature dependencies for maltose transport over the range of brewery temperatures (0–20 °C).

    abstract::Zero-trans rates of maltose transport by brewer's yeasts exert strong control over fermentation rates and are strongly temperature-dependent over the temperature range (20–0 °C) of brewery fermentations. Three α-glucoside transporters, ScAgt1(A60) (a Saccharomyces cerevisiae version of Agt1 from an ale strain), ScAgt1...

    journal_title:FEMS yeast research

    pub_type: 杂志文章

    doi:10.1111/1567-1364.12147

    authors: Vidgren V,Viljanen K,Mattinen L,Rautio J,Londesborough J

    更新日期:2014-06-01 00:00:00

  • Hierarchical and metabolic regulation of glucose influx in starved Saccharomyces cerevisiae.

    abstract::A novel method dissecting the regulation of a cellular function into direct metabolic regulation and hierarchical (e.g., gene-expression) regulation is applied to yeast starved for nitrogen or carbon. Upon nitrogen starvation glucose influx is down-regulated hierarchically. Upon carbon starvation it is down-regulated ...

    journal_title:FEMS yeast research

    pub_type: 杂志文章

    doi:10.1016/j.femsyr.2004.11.003

    authors: Rossell S,van der Weijden CC,Kruckeberg AL,Bakker BM,Westerhoff HV

    更新日期:2005-04-01 00:00:00

  • Comparative study of Saccharomyces cerevisiae wine strains to identify potential marker genes correlated to desiccation stress tolerance.

    abstract::The most diffused formulation of starter for winemaking is active dry yeast (ADY). ADYs production process is essentially characterized by air-drying stress, a combination of several stresses, including thermal, hyperosmotic and oxidative and cell capacity to counteract such multiple stresses will determine its surviv...

    journal_title:FEMS yeast research

    pub_type: 杂志文章

    doi:10.1093/femsyr/fow015

    authors: Capece A,Votta S,Guaragnella N,Zambuto M,Romaniello R,Romano P

    更新日期:2016-05-01 00:00:00

  • The F1-ATPase inhibitor Inh1 (IF1) affects suppression of mtDNA loss-lethality in Kluyveromyces lactis.

    abstract::Loss of mtDNA by the petite-negative yeast Kluyveromyces lactis is lethal (rho(o)-lethality). However, mutations in the alpha, beta and gamma subunits of F(1)-ATPase can suppress lethality by increasing intramitochondrial hydrolysis of ATP. Increased hydrolysis of ATP can also occur on inactivation of Inh1, the natura...

    journal_title:FEMS yeast research

    pub_type: 杂志文章

    doi:10.1111/j.1567-1364.2006.00201.x

    authors: Clark-Walker GD

    更新日期:2007-08-01 00:00:00

  • Yarrowia lipolytica: a model yeast for citric acid production.

    abstract::Every year more than 2 million tons of citric acid (CA) are produced around the world for industrial uses. Although initially extracted from citrus, the low profitability of the process and the increasing demand soon stimulated the search for more efficient methods to produce CA. Currently, most world CA demand (99%) ...

    journal_title:FEMS yeast research

    pub_type: 杂志文章

    doi:10.1093/femsyr/fox084

    authors: Cavallo E,Charreau H,Cerrutti P,Foresti ML

    更新日期:2017-12-01 00:00:00

  • Influence of climatic conditions on the isolation of members of the Cryptococcus neoformans species complex from trees in Colombia from 1992-2004.

    abstract::The aim of this retrospective study was to analyze the relationship between occurrence of the serotypes of the Cryptococcus neoformans species complex in tree samples and the climatic conditions registered during samplings in four cities of Colombia, between 1992 and 2004, by means of a logistic regression model and l...

    journal_title:FEMS yeast research

    pub_type: 杂志文章

    doi:10.1111/j.1567-1364.2006.00090.x

    authors: Granados DP,Castañeda E

    更新日期:2006-06-01 00:00:00

  • Yeast diversity in rice-cassava fermentations produced by the indigenous Tapirapé people of Brazil.

    abstract::The Tapirapé people of the Tapi'itãwa tribe of Brazil produce several fermented foods and beverages, one of which is called 'cauim'. This beverage usually makes up the main staple food for adults and children. Several substrates are used in its production, including cassava, rice, corn, maize and peanuts. A fermentati...

    journal_title:FEMS yeast research

    pub_type: 杂志文章

    doi:10.1111/j.1567-1364.2007.00241.x

    authors: Schwan RF,Almeida EG,Souza-Dias MA,Jespersen L

    更新日期:2007-09-01 00:00:00

  • Dimethyl sulphoxide modifies growth and senescence and induces the non-revertible petite phenotype in yeast.

    abstract::Dimethyl sulphoxide is extensively used in chemical, pharmaceutical and biomedical applications, but its specific biological actions remain largely elusive. The aim of this study was to comprehensively explore the effects of dimethyl sulphoxide on eukaryotic growth and senescence by using the budding yeast Saccharomyc...

    journal_title:FEMS yeast research

    pub_type: 杂志文章

    doi:10.1093/femsyr/fow008

    authors: Kakolyri M,Margaritou A,Tiligada E

    更新日期:2016-03-01 00:00:00

  • Metabolic response to MMS-mediated DNA damage in Saccharomyces cerevisiae is dependent on the glucose concentration in the medium.

    abstract::Maintenance and adaptation of energy metabolism could play an important role in the cellular ability to respond to DNA damage. A large number of studies suggest that the sensitivity of cells to oxidants and oxidative stress depends on the activity of cellular metabolism and is dependent on the glucose concentration. I...

    journal_title:FEMS yeast research

    pub_type: 杂志文章

    doi:10.1111/j.1567-1364.2009.00505.x

    authors: Kitanovic A,Walther T,Loret MO,Holzwarth J,Kitanovic I,Bonowski F,Van Bui N,Francois JM,Wölfl S

    更新日期:2009-06-01 00:00:00

  • Physiological responses to acid stress by Saccharomyces cerevisiae when applying high initial cell density.

    abstract::High initial cell density is used to increase volumetric productivity and shorten production time in lignocellulosic hydrolysate fermentation. Comparison of physiological parameters in high initial cell density cultivation of Saccharomyces cerevisiae in the presence of acetic, formic, levulinic and cinnamic acids demo...

    journal_title:FEMS yeast research

    pub_type: 杂志文章

    doi:10.1093/femsyr/fow072

    authors: Guo ZP,Olsson L

    更新日期:2016-11-01 00:00:00

  • Methionine catabolism in Saccharomyces cerevisiae.

    abstract::The catabolism of methionine to methionol and methanethiol in Saccharomyces cerevisiae was studied using (13)C NMR spectroscopy, GC-MS, enzyme assays and a number of mutants. Methionine is first transaminated to alpha-keto-gamma-(methylthio)butyrate. Methionol is formed by a decarboxylation reaction, which yields meth...

    journal_title:FEMS yeast research

    pub_type: 杂志文章

    doi:10.1111/j.1567-1356.2005.00005.x

    authors: Perpète P,Duthoit O,De Maeyer S,Imray L,Lawton AI,Stavropoulos KE,Gitonga VW,Hewlins MJ,Dickinson JR

    更新日期:2006-01-01 00:00:00

  • Characterization of a biofilm-like extracellular matrix in FLO1-expressing Saccharomyces cerevisiae cells.

    abstract::Like bacteria, fungi growing in biofilms are often embedded in a so-called extracellular matrix (ECM), a complex and species-specific mixture of compounds secreted by cells in the biofilm. The precise physiological role of this ECM and its importance for the stress and drug resistance that is so characteristic of biof...

    journal_title:FEMS yeast research

    pub_type: 杂志文章

    doi:10.1111/j.1567-1364.2009.00482.x

    authors: Beauvais A,Loussert C,Prevost MC,Verstrepen K,Latgé JP

    更新日期:2009-05-01 00:00:00

  • Phenotypic evaluation and characterization of 21 industrial Saccharomyces cerevisiae yeast strains.

    abstract::Microorganisms have been studied and used extensively to produce value-added fuels and chemicals. Yeasts, specifically Saccharomyces cerevisiae, receive industrial attention because of their well-known ability to ferment glucose and produce ethanol. Thousands of natural or genetically modified S. cerevisiae have been ...

    journal_title:FEMS yeast research

    pub_type: 杂志文章

    doi:10.1093/femsyr/foy001

    authors: Kong II,Turner TL,Kim H,Kim SR,Jin YS

    更新日期:2018-02-01 00:00:00

  • Genetic engineering of brewing yeast to reduce the content of ethanol in beer.

    abstract::The GPD1 gene encoding the glycerol-3-phosphate dehydrogenase was overexpressed in an industrial lager brewing yeast (Saccharomyces cerevisiae ssp. carlsbergensis) to reduce the content of ethanol in beer. The amount of glycerol produced by the GPD1-overexpressing yeast in fermentation experiments simulating brewing c...

    journal_title:FEMS yeast research

    pub_type: 杂志文章

    doi:10.1111/j.1567-1364.2002.tb00087.x

    authors: Nevoigt E,Pilger R,Mast-Gerlach E,Schmidt U,Freihammer S,Eschenbrenner M,Garbe L,Stahl U

    更新日期:2002-05-01 00:00:00

  • Development of a genetic transformation toolkit for Brettanomyces bruxellensis.

    abstract::Brettanomyces bruxellensis is usually considered a spoilage microorganism, responsible for significant economic losses during the production of fermented beverages such as wine, beer and cider, though for some styles of beer its influence is essential. In recent years, the competitiveness of this yeast in bioethanol p...

    journal_title:FEMS yeast research

    pub_type: 杂志文章

    doi:10.1093/femsyr/foy070

    authors: Varela C,Lleixà J,Curtin C,Borneman A

    更新日期:2018-11-01 00:00:00

  • Genetic variants of Malassezia pachydermatis from canine skin: body distribution and phospholipase activity.

    abstract::Malassezia pachydermatis isolates (n=185) from skin sites from dogs (n=30) were characterized genetically and biochemically following in vitro culture. Two regions in the chitin synthase-2 gene (chs-2) and the first internal transcribed spacer (ITS-1) of nuclear ribosomal DNA were sequenced, and the phospholipase acti...

    journal_title:FEMS yeast research

    pub_type: 杂志文章

    doi:10.1111/j.1567-1364.2008.00358.x

    authors: Cafarchia C,Gasser RB,Latrofa MS,Parisi A,Campbell BE,Otranto D

    更新日期:2008-05-01 00:00:00

  • Astaxanthin biosynthesis is enhanced by high carotenogenic gene expression and decrease of fatty acids and ergosterol in a Phaffia rhodozyma mutant strain.

    abstract::An astaxanthin-overproducing (∼1000 μg g(-1)) strain of Phaffia rhodozyma, termed MK19, was established through 1-methyl-3-nitro-1-nitrosoguanidine and Co60 mutagenesis from wild-type JCM9042 (merely 35-67 μg g(-1)). The total fatty acid content of MK19 was much lower than that of the wild type. Possible causes of the...

    journal_title:FEMS yeast research

    pub_type: 杂志文章

    doi:10.1111/j.1567-1364.2010.00705.x

    authors: Miao L,Chi S,Tang Y,Su Z,Yin T,Guan G,Li Y

    更新日期:2011-03-01 00:00:00

  • Ethanol reduces mitochondrial membrane integrity and thereby impacts carbon metabolism of Saccharomyces cerevisiae.

    abstract::Saccharomyces cerevisiae is an excellent ethanol producer, but is rather sensitive to high concentration of ethanol. Here, influences of ethanol on cellular membrane integrity and carbon metabolism of S. cerevisiae were investigated to rationalize mechanism involved in ethanol toxicity. Addition of 5% (v/v) ethanol di...

    journal_title:FEMS yeast research

    pub_type: 杂志文章

    doi:10.1111/j.1567-1364.2012.00818.x

    authors: Yang KM,Lee NR,Woo JM,Choi W,Zimmermann M,Blank LM,Park JB

    更新日期:2012-09-01 00:00:00

  • Elucidating cellular mechanisms of Saccharomyces cerevisiae tolerant to combined lignocellulosic-derived inhibitors using high-throughput phenotyping and multiomics analyses.

    abstract::A robust cell factory that can tolerate combined inhibitory lignocellulosic compounds is essential for the cost-effective lignocellulose-based production of second-generation bioethanol and other bulk chemicals. Following high-throughput phenotyping of a yeast genomic overexpression library, we identified a Saccharomy...

    journal_title:FEMS yeast research

    pub_type: 杂志文章

    doi:10.1093/femsyr/foy106

    authors: Unrean P,Gätgens J,Klein B,Noack S,Champreda V

    更新日期:2018-12-01 00:00:00

  • Heat shock-inducible expression vectors for use in Schizosaccharomyces pombe.

    abstract::A new, heat shock-inducible expression system based on an endogenous hsp16+ promoter was developed for use in the fission yeast Schizosaccharomyces pombe. Analysis of GFP expression profiles indicated that a 1.2-kb segment of the hsp16+ promoter region was sufficient to drive expression of heterologous protein. The hs...

    journal_title:FEMS yeast research

    pub_type: 杂志文章

    doi:10.1111/j.1567-1364.2006.00093.x

    authors: Fujita Y,Tohda H,Giga-Hama Y,Takegawa K

    更新日期:2006-09-01 00:00:00

  • A novel assay for replicative lifespan in Saccharomyces cerevisiae.

    abstract::The replicative lifespan of Saccharomyces cerevisiae is determined by both genetic and environmental factors. Many of the same factors determine the lifespan of metazoan animals. The lack of fast and reliable lifespan assays has limited the pace of yeast aging research. In this study we describe a novel strategy for a...

    journal_title:FEMS yeast research

    pub_type: 杂志文章

    doi:10.1016/j.femsyr.2004.06.015

    authors: Jarolim S,Millen J,Heeren G,Laun P,Goldfarb DS,Breitenbach M

    更新日期:2004-11-01 00:00:00

  • The effect of fungicides on yeast communities associated with grape berries.

    abstract::The influence of three commonly used fungicides (iprodione, pyrimethanil and fludioxonil plus cyprodinil) on the density and diversity of yeast populations present on grape berries was evaluated. At the time of harvest, the fungicide residues on grapes were below the maximum permitted levels. In general, larger yeast ...

    journal_title:FEMS yeast research

    pub_type: 杂志文章

    doi:10.1111/j.1567-1364.2010.00635.x

    authors: Cadez N,Zupan J,Raspor P

    更新日期:2010-08-01 00:00:00

  • Cell size and morphological properties of yeast Saccharomyces cerevisiae in relation to growth temperature.

    abstract::Cell volume is an important parameter for modelling cellular processes. Temperature-induced variability of cellular size, volume, intracellular granularity, a fraction of budding cells of yeast Saccharomyces cerevisiae CEN.PK 113-7D (in anaerobic glucose unlimited batch cultures) were measured by flow cytometry and ma...

    journal_title:FEMS yeast research

    pub_type: 杂志文章

    doi:10.1093/femsyr/foy052

    authors: Zakhartsev M,Reuss M

    更新日期:2018-09-01 00:00:00

  • Alternative clamp loaders/unloaders.

    abstract::Each time a cell duplicates, the whole genome must be accurately copied and distributed. The enormous amount of DNA in eukaryotic cells requires a high level of coordination between polymerases and other DNA and chromatin-interacting proteins to ensure timely and accurate DNA replication and chromatin formation. PCNA ...

    journal_title:FEMS yeast research

    pub_type: 杂志文章,评审

    doi:10.1093/femsyr/fow084

    authors: Kupiec M

    更新日期:2016-11-01 00:00:00