Abstract：
:Every year more than 2 million tons of citric acid (CA) are produced around the world for industrial uses. Although initially extracted from citrus, the low profitability of the process and the increasing demand soon stimulated the search for more efficient methods to produce CA. Currently, most world CA demand (99%) is satisfied by fermentations with microorganisms, especially filamentous fungi and yeasts. CA production with yeasts has certain advantages over molds (e.g. higher productivity and easier cultivation), which in the last two decades have triggered a clear increase in publications and patents devoted to the use of yeasts in this field. Yarrowia lipolytica has become a model yeast that proved to be successful in different production systems. Considering the current interest evidenced in the literature, the most significant information on CA production using Y. lipolytica is summarized. The relevance on CA yields of key factors such as strains, media formulation, environmental conditions and production regimes is thoroughly discussed, with particular focus on increasing CA productivity. Besides, the possibility of tuning the mentioned variables to reduce concomitant isocitric acid production-the biggest disadvantage of using yeasts-is analyzed. Available methods for CA purification/quantification are also discussed.
journal_name
FEMS Yeast Resjournal_title
FEMS yeast researchauthors
Cavallo E,Charreau H,Cerrutti P,Foresti MLdoi
10.1093/femsyr/fox084subject
Has Abstractpub_date
2017-12-01 00:00:00issue
8eissn
1567-1356issn
1567-1364pii
4587737journal_volume
17pub_type
杂志文章abstract：:Dysfunction or capacity shortage of the endoplasmic reticulum (ER) is cumulatively called ER stress and provokes the unfolded protein response (UPR). In various yeast species, the ER-located transmembrane protein Ire1 is activated upon ER stress and performs the splicing reaction of HAC1 mRNA, the mature form of which...
journal_title：FEMS yeast research
pub_type： 杂志文章
doi：10.1093/femsyr/foaa053
更新日期：2020-10-15 00:00:00
abstract：:Secreted lipases of Candida albicans are encoded by a gene family with at least 10 members (LIP1-LIP10). The expression pattern of this multigene family was investigated using reverse transcription polymerase chain reaction in experimental infections and in samples of patients suffering from oral candidosis. The findi...
journal_title：FEMS yeast research
pub_type： 杂志文章
doi：10.1016/S1567-1356(03)00205-8
更新日期：2004-01-01 00:00:00
abstract：:Proteostasis is a cellular housekeeping process that refers to the healthy maintenance of the cellular proteome that governs the fate of proteins from synthesis to degradation. Perturbations of proteostasis might result in protein dysfunction with consequent deleterious effects that can culminate in cell death. To dea...
journal_title：FEMS yeast research
pub_type： 杂志文章,评审
doi：10.1093/femsyr/foy043
更新日期：2018-08-01 00:00:00
abstract：:The microbial consortium of wine fermentations is highly dependent upon winemaking decisions made at crush, including the decision to inoculate and the decision to add sulfur dioxide (SO2) to the must. To investigate this, Chardonnay grape juice was subjected to two inoculation treatments (uninoculated and pied de cuv...
journal_title：FEMS yeast research
pub_type： 杂志文章
doi：10.1093/femsyr/foz049
更新日期：2019-08-01 00:00:00
abstract：:Zero-trans rates of maltose transport by brewer's yeasts exert strong control over fermentation rates and are strongly temperature-dependent over the temperature range (20–0 °C) of brewery fermentations. Three α-glucoside transporters, ScAgt1(A60) (a Saccharomyces cerevisiae version of Agt1 from an ale strain), ScAgt1...
journal_title：FEMS yeast research
pub_type： 杂志文章
doi：10.1111/1567-1364.12147
更新日期：2014-06-01 00:00:00
abstract：:Molecular tools for the production of heterologous proteins and metabolic engineering applications of the non-conventional yeast Zygosaccharomyces bailii were developed. The combination of Z. bailii's resistance to relatively high temperature, osmotic pressure and low pH values, with a high specific growth rate render...
journal_title：FEMS yeast research
pub_type： 杂志文章
doi：10.1016/S1567-1356(03)00200-9
更新日期：2004-01-01 00:00:00
abstract：:Lignocellulosic plant biomass is the target feedstock for production of second-generation biofuels. Ionic liquid (IL) pretreatment can enhance deconstruction of lignocellulosic biomass into sugars that can be fermented to ethanol. Although biomass is typically washed following IL pretreatment, small quantities of resi...
journal_title：FEMS yeast research
pub_type： 杂志文章
doi：10.1111/1567-1364.12224
更新日期：2014-12-01 00:00:00
abstract：:Candida glabrata is a second most common human opportunistic pathogen which causes superficial but also life-threatening systemic candidosis. According to the localisation of mannans and mannoproteins in the outermost layer of the cell wall, mannan detection could be one of the first steps in the cell recognition of C...
journal_title：FEMS yeast research
pub_type： 杂志文章
doi：10.1093/femsyr/foz009
更新日期：2019-03-01 00:00:00
abstract：:Homologous recombination (HR) is an error-free DNA repair mechanism that maintains genome integrity by repairing double-strand breaks (DSBs). Defects in HR lead to genomic instability and are associated with cancer predisposition. A key step in HR is the formation of Rad51 nucleoprotein filaments which are responsible...
journal_title：FEMS yeast research
pub_type： 杂志文章
doi：10.1093/femsyr/fow073
更新日期：2016-09-01 00:00:00
abstract：:The yeast Zygosaccharomyces bailii, known to have peculiar resistance to several environmental constraints, is very little known with respect to its genetics and life cycle. In addition to molecular and biochemical studies, cytofluorimetric and morphological analyses can also add information necessary to shed light on...
journal_title：FEMS yeast research
pub_type： 杂志文章
doi：10.1111/j.1567-1364.2008.00417.x
更新日期：2008-09-01 00:00:00
abstract：:The FLO11-encoded flocculin is required for a variety of important phenotypes in Saccharomyces cerevisiae, including flocculation, adhesion to agar and plastic, invasive growth, pseudohyphae formation and biofilm development. We present evidence that Flo11p belongs to the Flo1-type class of flocculins rather than to t...
journal_title：FEMS yeast research
pub_type： 杂志文章
doi：10.1016/j.femsyr.2005.05.004
更新日期：2005-12-01 00:00:00
abstract：:The mechanistic details of mtDNA maintenance in petite-negative yeasts have remained largely unexplored. We report here that the DNA helicase Hmi1p plays a crucial role in mtDNA stability in Candida albicans. Like its counterpart in Saccharomyces cerevisiae, Hmi1p in C. albicans (CaHmi1p) contains a C-terminal mitocho...
journal_title：FEMS yeast research
pub_type： 杂志文章
doi：10.1111/j.1567-1364.2006.00132.x
更新日期：2007-01-01 00:00:00
abstract：:The roles of intracellular GTPase Arl1 and organellar cation/H(+) antiporters (Kha1 and Nhx1) in Saccharomyces cerevisiae tolerance to various stress factors were investigated and interesting new phenotypes of strains devoid of these proteins were found. The role of Arl1 GTPase in their tolerance to various cations is...
journal_title：FEMS yeast research
pub_type： 杂志文章
doi：10.1111/j.1567-1364.2010.00661.x
更新日期：2010-11-01 00:00:00
abstract：:The feasibility of using a single vector to clone a cytochrome P450 monooxygenase (P450) in different yeasts and then compare whole-cell hydroxylase activity was investigated. A broad-range yeast expression vector using the ylTEFp to drive expression of the cloned gene and the scTEFp to drive the hygromycin resistance...
journal_title：FEMS yeast research
pub_type： 杂志文章
doi：10.1111/1567-1364.12142
更新日期：2014-06-01 00:00:00
abstract：:Schizosaccharomyces pombe Cap1 has been identified as the (adenylyl) cyclase-associated protein. Cap1 was able to bind Cap1 itself and actin. Cap1 localized at the growing tip, and this localization was dependent on the Cap1 P2 region. In a two-hybrid screening using cap1 as bait, we isolated csh3, which encodes a pro...
journal_title：FEMS yeast research
pub_type： 杂志文章
doi：10.1093/femsyr/fov097
更新日期：2015-12-01 00:00:00
abstract：:The transport of glucose and fructose into yeast cells is a critical step in the utilization of sugars during wine fermentation. Hexose uptake can be carried out by various Hxt carriers, each possessing distinct regulatory and transport-kinetic properties capable of influencing yeast fermentation capacity. We investig...
journal_title：FEMS yeast research
pub_type： 杂志文章
doi：10.1016/j.femsyr.2004.09.005
更新日期：2005-02-01 00:00:00
abstract：:Trk1 and Trk2 are the major K(+) transport systems in Schizosaccharomyces pombe. Both transporters individually seem to be able to cope with K(+) requirements of the cells under normal conditions, since only the double mutant shows defective K(+) transport and defective growth at limiting K(+) concentrations. We have ...
journal_title：FEMS yeast research
pub_type： 杂志文章
doi：10.1016/j.femsyr.2003.11.003
更新日期：2004-03-01 00:00:00
abstract：:Each time a cell duplicates, the whole genome must be accurately copied and distributed. The enormous amount of DNA in eukaryotic cells requires a high level of coordination between polymerases and other DNA and chromatin-interacting proteins to ensure timely and accurate DNA replication and chromatin formation. PCNA ...
journal_title：FEMS yeast research
pub_type： 杂志文章,评审
doi：10.1093/femsyr/fow084
更新日期：2016-11-01 00:00:00
abstract：:The progenitor of the Dekkera/Brettanomyces clade separated from the Saccharomyces/Kluyveromyces clade over 200 million years ago. However, within both clades, several lineages developed similar physiological traits. Both Saccharomyces cerevisiae and Dekkera bruxellensis are facultative anaerobes; in the presence of e...
journal_title：FEMS yeast research
pub_type： 杂志文章
doi：10.1111/j.1567-1364.2010.00644.x
更新日期：2010-08-01 00:00:00
abstract：:To investigate the role of the prevacuolar secretion pathway in the trafficking of vacuolar proteins in Candida albicans, the C. albicans homolog of the Saccharomyces cerevisiae vacuolar protein sorting gene VPS4 was cloned and analyzed. Candida albicans VPS4 encodes a deduced AAA-type ATPase that is 75.6% similar to ...
journal_title：FEMS yeast research
pub_type： 杂志文章
doi：10.1111/j.1567-1364.2007.00253.x
更新日期：2007-09-01 00:00:00
abstract：:We used multi-locus sequence typing (MLST) to investigate 35 yeast isolates representing the two genome-sequenced strains plus the type strain of Candida albicans, four isolates originally identified as Candida stellatoidea type I and 28 representing type strains of other species now regarded as synonymous with C. alb...
journal_title：FEMS yeast research
pub_type： 杂志文章
doi：10.1111/j.1567-1364.2008.00392.x
更新日期：2008-08-01 00:00:00
abstract：:Non-Saccharomyces yeasts impact wine fermentations and can diversify the flavor profiles of wines. However, little information is available on the metabolic networks of most of these species. Here we show that unlike the main wine yeast Saccharomyces cerevisiae, Torulaspora delbrueckii and to a lesser extent Lachancea...
journal_title：FEMS yeast research
pub_type： 杂志文章
doi：10.1093/femsyr/foy084
更新日期：2018-11-01 00:00:00
abstract：:Endosomal sorting complexes required for transport (ESCRTs) are involved in the formation of multivesicular bodies and sorting of targeted proteins to the yeast vacuole. The deletion of seven genes encoding components of the ESCRT machinery render Saccharomyces cerevisiae cells sensitive to high extracellular CaCl2 co...
journal_title：FEMS yeast research
pub_type： 杂志文章
doi：10.1093/femsyr/fov007
更新日期：2015-03-01 00:00:00
abstract：:In the pathogenic fungus Candida glabrata, the YPS1 gene, which encodes a glycosylphosphatidylinositol-linked aspartyl protease, is required for cell wall integrity and virulence. Although the expression of YPS1 has been studied in Saccharomyces cerevisiae, the transcriptional regulation of this gene in C. glabrata is...
journal_title：FEMS yeast research
pub_type： 杂志文章
doi：10.1111/j.1567-1364.2011.00734.x
更新日期：2011-08-01 00:00:00
abstract：:The yeast vacuolar membrane protein Ycf1p and its mammalian counterpart, MRP1, belong to the ABCC subfamily of ATP-binding cassette transporters. Genetic evidence suggests that the yeast casein kinase 2α, Cka1p, negatively regulates Ycf1p function via phosphorylation of Ser251 within the N-terminus. In this study, we ...
journal_title：FEMS yeast research
pub_type： 杂志文章
doi：10.1111/j.1567-1364.2010.00677.x
更新日期：2010-11-01 00:00:00
abstract：:Since the discovery of the apoptotic pathway in Saccharomyces cerevisiae, several compounds have been shown to cause apoptosis in this organism. While the toxicity of polyunsaturated fatty acids (PUFA) peroxides towards S. cerevisiae has been known for a long time, studies on the effect of nonoxidized PUFA are scarce....
journal_title：FEMS yeast research
pub_type： 杂志文章
doi：10.1111/j.1567-1364.2011.00729.x
更新日期：2011-08-01 00:00:00
abstract：:Mitochondria are dynamic organelles of endosymbiotic origin that are essential components of eukaryal cells. They contain their own genetic machinery, have multicopy genomes and like their bacterial ancestors they consist of two membranes. However, the majority of the ancestral genome has been lost or transferred to t...
journal_title：FEMS yeast research
pub_type： 杂志文章,评审
doi：10.1093/femsyr/foy040
更新日期：2018-08-01 00:00:00
abstract：:The catabolism of methionine to methionol and methanethiol in Saccharomyces cerevisiae was studied using (13)C NMR spectroscopy, GC-MS, enzyme assays and a number of mutants. Methionine is first transaminated to alpha-keto-gamma-(methylthio)butyrate. Methionol is formed by a decarboxylation reaction, which yields meth...
journal_title：FEMS yeast research
pub_type： 杂志文章
doi：10.1111/j.1567-1356.2005.00005.x
更新日期：2006-01-01 00:00:00
abstract：:The aim of this retrospective study was to analyze the relationship between occurrence of the serotypes of the Cryptococcus neoformans species complex in tree samples and the climatic conditions registered during samplings in four cities of Colombia, between 1992 and 2004, by means of a logistic regression model and l...
journal_title：FEMS yeast research
pub_type： 杂志文章
doi：10.1111/j.1567-1364.2006.00090.x
更新日期：2006-06-01 00:00:00
abstract：:The transcriptional regulator Pdr1 plays a positive role in regulating azole drug resistance in Candida glabrata. Previous studies have shown the importance of the carboxyl (C)-terminal sequence of Pdr1 in fulfilling its function, as this region mediates interactions between Pdr1 and the co-activator Gal11A and is cru...
journal_title：FEMS yeast research
pub_type： 杂志文章
doi：10.1093/femsyr/foy038
更新日期：2018-06-01 00:00:00
