Abstract:
:Mitochondria are dynamic organelles of endosymbiotic origin that are essential components of eukaryal cells. They contain their own genetic machinery, have multicopy genomes and like their bacterial ancestors they consist of two membranes. However, the majority of the ancestral genome has been lost or transferred to the nuclear genome of the host, preserving only a core set of genes involved in oxidative phosphorylation. Mitochondria perform numerous biological tasks ranging from bioenergetics to production of protein co-factors, including heme and iron-sulfur clusters. Due to the importance of mitochondria in many cellular processes, mitochondrial dysfunction is implicated in a wide variety of human disorders. Much of our current knowledge on mitochondrial function and dysfunction comes from studies using Saccharomyces cerevisiae. This yeast has good fermenting capacity, rendering tolerance to mutations that inactivate oxidative phosphorylation and complete loss of mitochondrial DNA. Here, we review yeast mitochondrial metabolism and function with focus on S. cerevisiae and its contribution in understanding mitochondrial biology. We further review how systems biology studies, including mathematical modeling, has allowed gaining new insight into mitochondrial function, and argue that this approach may enable us to gain a holistic view on how mitochondrial function interacts with different cellular processes.
journal_name
FEMS Yeast Resjournal_title
FEMS yeast researchauthors
Malina C,Larsson C,Nielsen Jdoi
10.1093/femsyr/foy040subject
Has Abstractpub_date
2018-08-01 00:00:00issue
5eissn
1567-1356issn
1567-1364pii
4969682journal_volume
18pub_type
杂志文章,评审abstract::Candida lipolytica and Candida rugosa were isolated from blood samples from a patient with chronic myeloid leukemia (31 years old) and a patient with sickle cell disease (1-year-old), respectively. Isolates were grown for 48 h at 37 degrees C in either Sabouraud or tryptone soy broth (TSB). Peptidases were tested for ...
journal_title:FEMS yeast research
pub_type: 杂志文章
doi:10.1111/j.1567-1364.2007.00269.x
更新日期:2007-09-01 00:00:00
abstract::The mechanistic details of mtDNA maintenance in petite-negative yeasts have remained largely unexplored. We report here that the DNA helicase Hmi1p plays a crucial role in mtDNA stability in Candida albicans. Like its counterpart in Saccharomyces cerevisiae, Hmi1p in C. albicans (CaHmi1p) contains a C-terminal mitocho...
journal_title:FEMS yeast research
pub_type: 杂志文章
doi:10.1111/j.1567-1364.2006.00132.x
更新日期:2007-01-01 00:00:00
abstract::Here we present evidence that Hsp150/Pir2, a member of the Pir family of cell wall proteins, can be extracted from the purified cell walls of Saccharomyces cerevisiae by treatment with beta-mercaptoethanol, demonstrating that at least part of this protein is attached to the cell wall through disulfide bridges. We also...
journal_title:FEMS yeast research
pub_type: 杂志文章
doi:10.1111/j.1567-1364.2001.tb00040.x
更新日期:2001-12-01 00:00:00
abstract::The replicative lifespan of Saccharomyces cerevisiae is determined by both genetic and environmental factors. Many of the same factors determine the lifespan of metazoan animals. The lack of fast and reliable lifespan assays has limited the pace of yeast aging research. In this study we describe a novel strategy for a...
journal_title:FEMS yeast research
pub_type: 杂志文章
doi:10.1016/j.femsyr.2004.06.015
更新日期:2004-11-01 00:00:00
abstract::Amylases are used in a variety of industries that have a specific need for alternative enzymes capable of hydrolysing raw starch. Five α-amylase and five glucoamylase-encoding genes were expressed in the Saccharomyces cerevisiae Y294 laboratory strain to select for recombinant strains that best hydrolysed raw corn sta...
journal_title:FEMS yeast research
pub_type: 杂志文章
doi:10.1093/femsyr/foy127
更新日期:2019-03-01 00:00:00
abstract::Kluyveromyces marxianus is a safe yeast used in the food and biotechnology sectors. One of the important traits that sets it apart from the familiar yeasts, Saccharomyces cerevisiae, is its capacity to grow using lactose as a carbon source. Like in its close relative, Kluyveromyces lactis, this requires lactose transp...
journal_title:FEMS yeast research
pub_type: 杂志文章
doi:10.1093/femsyr/fox021
更新日期:2017-05-01 00:00:00
abstract::We used multi-locus sequence typing (MLST) to investigate 35 yeast isolates representing the two genome-sequenced strains plus the type strain of Candida albicans, four isolates originally identified as Candida stellatoidea type I and 28 representing type strains of other species now regarded as synonymous with C. alb...
journal_title:FEMS yeast research
pub_type: 杂志文章
doi:10.1111/j.1567-1364.2008.00392.x
更新日期:2008-08-01 00:00:00
abstract::Dimethyl sulphoxide is extensively used in chemical, pharmaceutical and biomedical applications, but its specific biological actions remain largely elusive. The aim of this study was to comprehensively explore the effects of dimethyl sulphoxide on eukaryotic growth and senescence by using the budding yeast Saccharomyc...
journal_title:FEMS yeast research
pub_type: 杂志文章
doi:10.1093/femsyr/fow008
更新日期:2016-03-01 00:00:00
abstract::Microorganisms have been studied and used extensively to produce value-added fuels and chemicals. Yeasts, specifically Saccharomyces cerevisiae, receive industrial attention because of their well-known ability to ferment glucose and produce ethanol. Thousands of natural or genetically modified S. cerevisiae have been ...
journal_title:FEMS yeast research
pub_type: 杂志文章
doi:10.1093/femsyr/foy001
更新日期:2018-02-01 00:00:00
abstract::Previous work has shown that the synthetic lethality of the slt2Δrim101Δ mutant results from a combination of factors, including improper functioning of the septum assembly machinery. Here, we identify new multicopy suppressors of this lethality including Kss1, Pcl1 and Sph1, none of which seems to be linked to the up...
journal_title:FEMS yeast research
pub_type: 杂志文章
doi:10.1093/femsyr/fow060
更新日期:2016-09-01 00:00:00
abstract::Trk1 and Trk2 are the major K(+) transport systems in Schizosaccharomyces pombe. Both transporters individually seem to be able to cope with K(+) requirements of the cells under normal conditions, since only the double mutant shows defective K(+) transport and defective growth at limiting K(+) concentrations. We have ...
journal_title:FEMS yeast research
pub_type: 杂志文章
doi:10.1016/j.femsyr.2003.11.003
更新日期:2004-03-01 00:00:00
abstract::We have reported previously that growth on alcohol vapors confers hemolytic properties on certain yeast species and strains ['microbial alcohol-conferred hemolysis' (MACH)]. In a recent study, we analyzed the genetic basis of MACH in Saccharomyces cerevisiae using the EUROSCARF mutant collection. The data suggested th...
journal_title:FEMS yeast research
pub_type: 杂志文章
doi:10.1111/j.1567-1364.2011.00722.x
更新日期:2011-06-01 00:00:00
abstract::Zero-trans rates of maltose transport by brewer's yeasts exert strong control over fermentation rates and are strongly temperature-dependent over the temperature range (20–0 °C) of brewery fermentations. Three α-glucoside transporters, ScAgt1(A60) (a Saccharomyces cerevisiae version of Agt1 from an ale strain), ScAgt1...
journal_title:FEMS yeast research
pub_type: 杂志文章
doi:10.1111/1567-1364.12147
更新日期:2014-06-01 00:00:00
abstract::High initial cell density is used to increase volumetric productivity and shorten production time in lignocellulosic hydrolysate fermentation. Comparison of physiological parameters in high initial cell density cultivation of Saccharomyces cerevisiae in the presence of acetic, formic, levulinic and cinnamic acids demo...
journal_title:FEMS yeast research
pub_type: 杂志文章
doi:10.1093/femsyr/fow072
更新日期:2016-11-01 00:00:00
abstract::Promoter of alcohol oxidase I (PAOX1) is the most efficient promoter involved in the regulation of recombinant protein expression in Pichia pastoris (P. pastoris). PAOX1 is tightly repressed by the presence of glycerol in the culture medium; thus, glycerol must be exhausted before methanol can be taken up by P. pastor...
journal_title:FEMS yeast research
pub_type: 杂志文章
doi:10.1093/femsyr/fow033
更新日期:2016-06-01 00:00:00
abstract::Killer-toxin complexes produced by Kluyveromyces lactis and Pichia acaciae inhibit cell proliferation of Saccharomyces cerevisiae. Analysis of their actions in haploid MATalpha cells revealed that introduction of the opposite mating-type locus (MATa) significantly suppressed antizymosis. Together with resistance expre...
journal_title:FEMS yeast research
pub_type: 杂志文章
doi:10.1111/j.1567-1364.2005.00006.x
更新日期:2006-05-01 00:00:00
abstract::The pathogenic yeast Cryptococcus neoformans has evolved several strategies to survive within phagocytes. Recently, it has been demonstrated that upregulation of the ATP binding cassette transporter-encoding gene antifungal resistance 1 (AFR1) is important not only for determining the resistance of C. neoformans to fl...
journal_title:FEMS yeast research
pub_type: 杂志文章
doi:10.1111/j.1567-1364.2008.00470.x
更新日期:2009-03-01 00:00:00
abstract::To analyse the reliability and accuracy of genotype analysis with high-density oligonucleotide microarrays, this method and other experimental approaches were used to analyse genomic DNA of two popular Saccharomyces cerevisiae laboratory strains. S288C was used for systematic sequencing of 'the' S. cerevisiae genome; ...
journal_title:FEMS yeast research
pub_type: 杂志文章
doi:10.1016/S1567-1356(03)00156-9
更新日期:2003-12-01 00:00:00
abstract::Maintenance and adaptation of energy metabolism could play an important role in the cellular ability to respond to DNA damage. A large number of studies suggest that the sensitivity of cells to oxidants and oxidative stress depends on the activity of cellular metabolism and is dependent on the glucose concentration. I...
journal_title:FEMS yeast research
pub_type: 杂志文章
doi:10.1111/j.1567-1364.2009.00505.x
更新日期:2009-06-01 00:00:00
abstract::The closely related yeasts Debaryomyces fabryi and Debaryomyces hansenii are excellent xylose consumers. We previously described the activity of a high-affinity xylose/H(+) symport from an industrial strain of D. hansenii subsequently reclassified as D. fabryi. We now report the identification of the gene encoding thi...
journal_title:FEMS yeast research
pub_type: 杂志文章
doi:10.1111/1567-1364.12061
更新日期:2013-11-01 00:00:00
abstract::In Kluyveromyces lactis, galactose transport has been thought to be mediated by the lactose permease encoded by LAC12. In fact, a lac12 mutant unable to grow on lactose did not grow on galactose either and showed low and uninducible galactose uptake activity. The existence of other galactose transport systems, at low ...
journal_title:FEMS yeast research
pub_type: 杂志文章
doi:10.1111/j.1567-1364.2006.00107.x
更新日期:2006-12-01 00:00:00
abstract::First-generation (1G) fuel ethanol production in sugarcane-based biorefineries is an established economic enterprise in Brazil. Second-generation (2G) fuel ethanol from lignocellulosic materials, though extensively investigated, is currently facing severe difficulties to become economically viable. Some of the challen...
journal_title:FEMS yeast research
pub_type: 杂志文章
doi:10.1093/femsyr/foaa027
更新日期:2020-06-01 00:00:00
abstract::Saccharomyces cerevisiae is the most widely used fermentation organism for ethanol production. However, the gene expression regulatory networks behind the ethanol fermentation are still not fully understood. Using a static fermentation model, we examined the ethanol yields on biomass of deletion mutants for 77 yeast g...
journal_title:FEMS yeast research
pub_type: 杂志文章
doi:10.1093/femsyr/fow022
更新日期:2016-05-01 00:00:00
abstract::During their mitotic cycle, cylindrical fission yeast cells grow exclusively at their tips. Length growth starts at birth and halts at mitotic onset when the cells begin to prepare for division. While the growth pattern was initially considered to be exponential, during the last three decades an increasing amount of e...
journal_title:FEMS yeast research
pub_type: 杂志文章
doi:10.1111/1567-1364.12064
更新日期:2013-11-01 00:00:00
abstract::Malassezia pachydermatis isolates (n=185) from skin sites from dogs (n=30) were characterized genetically and biochemically following in vitro culture. Two regions in the chitin synthase-2 gene (chs-2) and the first internal transcribed spacer (ITS-1) of nuclear ribosomal DNA were sequenced, and the phospholipase acti...
journal_title:FEMS yeast research
pub_type: 杂志文章
doi:10.1111/j.1567-1364.2008.00358.x
更新日期:2008-05-01 00:00:00
abstract::We studied the effect of the loss of the Ser-Thr protein phosphatase Sit4, an important post-translational regulator, on the steady-state levels of the low-affinity glucose transporter Hxt1p and observed a delay in its appearance after high glucose induction, slow growth, and diminished glucose consumption. By analyzi...
journal_title:FEMS yeast research
pub_type: 杂志文章
doi:10.1111/j.1567-1364.2012.00839.x
更新日期:2012-12-01 00:00:00
abstract::An astaxanthin-overproducing (∼1000 μg g(-1)) strain of Phaffia rhodozyma, termed MK19, was established through 1-methyl-3-nitro-1-nitrosoguanidine and Co60 mutagenesis from wild-type JCM9042 (merely 35-67 μg g(-1)). The total fatty acid content of MK19 was much lower than that of the wild type. Possible causes of the...
journal_title:FEMS yeast research
pub_type: 杂志文章
doi:10.1111/j.1567-1364.2010.00705.x
更新日期:2011-03-01 00:00:00
abstract::Development of a pulsed-light (PL) approach to inanimate surface decontamination is timely, as the incidence of yeast-related infections in healthcare remains unacceptably high. Critical electrical and biological factors governing the efficacy of PL for the in vitro inactivation of medically important yeast were estab...
journal_title:FEMS yeast research
pub_type: 杂志文章
doi:10.1111/j.1567-1364.2009.00543.x
更新日期:2009-09-01 00:00:00
abstract::This study addresses the question of whether observable changes in fluxes in the primary carbon metabolism of Saccharomyces cerevisiae occur between the different phases of the cell division cycle. To detect such changes by metabolic flux analysis, a 13C-labeling experiment was performed with a fed-batch culture inocu...
journal_title:FEMS yeast research
pub_type: 杂志文章
doi:10.1111/j.1567-1364.2006.00199.x
更新日期:2007-06-01 00:00:00
abstract::We show that Arf3p, a member of the ADP ribosylation family, is involved in the organization of actin cables and cortical patches in Saccharomyces cerevisiae. Profilin-deficient cells (pfy1Delta) have severe growth defects and lack actin cables. Overexpression of ARF3 restores actin cables and corrects growth defects ...
journal_title:FEMS yeast research
pub_type: 杂志文章
doi:10.1111/j.1567-1364.2007.00239.x
更新日期:2007-09-01 00:00:00