Phenotypic evaluation and characterization of 21 industrial Saccharomyces cerevisiae yeast strains.

abstract：:First-generation (1G) fuel ethanol production in sugarcane-based biorefineries is an established economic enterprise in Brazil. Second-generation (2G) fuel ethanol from lignocellulosic materials, though extensively investigated, is currently facing severe difficulties to become economically viable. Some of the challen...

journal_title：FEMS yeast research

authors： Bermejo PM,Raghavendran V,Gombert AK

更新日期：2020-06-01 00:00:00

abstract：:During their mitotic cycle, cylindrical fission yeast cells grow exclusively at their tips. Length growth starts at birth and halts at mitotic onset when the cells begin to prepare for division. While the growth pattern was initially considered to be exponential, during the last three decades an increasing amount of e...

journal_title：FEMS yeast research

authors： Horváth A,Rácz-Mónus A,Buchwald P,Sveiczer Á

更新日期：2013-11-01 00:00:00

abstract：:An astaxanthin-overproducing (∼1000 μg g(-1)) strain of Phaffia rhodozyma, termed MK19, was established through 1-methyl-3-nitro-1-nitrosoguanidine and Co60 mutagenesis from wild-type JCM9042 (merely 35-67 μg g(-1)). The total fatty acid content of MK19 was much lower than that of the wild type. Possible causes of the...

journal_title：FEMS yeast research

authors： Miao L,Chi S,Tang Y,Su Z,Yin T,Guan G,Li Y

更新日期：2011-03-01 00:00:00

abstract：:The transcriptional regulator Pdr1 plays a positive role in regulating azole drug resistance in Candida glabrata. Previous studies have shown the importance of the carboxyl (C)-terminal sequence of Pdr1 in fulfilling its function, as this region mediates interactions between Pdr1 and the co-activator Gal11A and is cru...

journal_title：FEMS yeast research

authors： Tian Y,Gao N,Ni Q,Mao Y,Dong D,Huang X,Jiang C,Li Z,Zhang L,Wang X,Peng Y,Chen C

更新日期：2018-06-01 00:00:00

abstract：:Zinc is a crucial mineral for all organisms as it is an essential cofactor for the proper function of a plethora of proteins and depletion of zinc causes oxidative stress. Glutathione is the major redox buffering agent in the cell and therefore important for mitigation of the adverse effects of oxidative stress. In ma...

journal_title：FEMS yeast research

authors： Steiger MG,Patzschke A,Holz C,Lang C,Causon T,Hann S,Mattanovich D,Sauer M

更新日期：2017-06-01 00:00:00

abstract：:The transport of glucose and fructose into yeast cells is a critical step in the utilization of sugars during wine fermentation. Hexose uptake can be carried out by various Hxt carriers, each possessing distinct regulatory and transport-kinetic properties capable of influencing yeast fermentation capacity. We investig...

journal_title：FEMS yeast research

authors： Perez M,Luyten K,Michel R,Riou C,Blondin B

更新日期：2005-02-01 00:00:00

abstract：:Mitogen activated protein kinase (MAPK) cascades are signal transduction mechanisms present in eukaryotic cells that allow adaptation to environmental changes. MAPK activity is mainly regulated by dual phosphorylation in a TXY motif present in the kinase subdomain VIII as well as dephosphorylation by specific phosphat...

journal_title：FEMS yeast research

authors： Román E,Alonso-Monge R,Gong Q,Li D,Calderone R,Pla J

更新日期：2009-09-01 00:00:00

abstract：:Zero-trans rates of maltose transport by brewer's yeasts exert strong control over fermentation rates and are strongly temperature-dependent over the temperature range (20–0 °C) of brewery fermentations. Three α-glucoside transporters, ScAgt1(A60) (a Saccharomyces cerevisiae version of Agt1 from an ale strain), ScAgt1...

journal_title：FEMS yeast research

authors： Vidgren V,Viljanen K,Mattinen L,Rautio J,Londesborough J

更新日期：2014-06-01 00:00:00

abstract：:The biosynthesis of yeast phosphatidylglycerol (PG) takes place in the inner mitochondrial membrane. Outside mitochondria, the abundance of PG is low. Here, we present evidence that the subcellular distribution of PG is maintained by the locally controlled enzymatic activity of the PG-specific phospholipase, Pgc1. A f...

journal_title：FEMS yeast research

authors： Kubalová D,Káňovičová P,Veselá P,Awadová T,Džugasová V,Daum G,Malínský J,Balážová M

更新日期：2019-08-01 00:00:00

abstract：:Malassezia pachydermatis isolates (n=185) from skin sites from dogs (n=30) were characterized genetically and biochemically following in vitro culture. Two regions in the chitin synthase-2 gene (chs-2) and the first internal transcribed spacer (ITS-1) of nuclear ribosomal DNA were sequenced, and the phospholipase acti...

journal_title：FEMS yeast research

authors： Cafarchia C,Gasser RB,Latrofa MS,Parisi A,Campbell BE,Otranto D

更新日期：2008-05-01 00:00:00

abstract：:A genetic and cell-biological analysis is provided for Saccharomyces cerevisiae DML1 (YMR211w) encoding a Drosophila melanogaster Misato-like protein. Misato and Dml1p are descendants of an ancestral tubulin-like protein, and exhibit regions with similarity to members of a GTPase family that include eukaryotic tubulin...

journal_title：FEMS yeast research

authors： Gurvitz A,Hartig A,Ruis H,Hamilton B,de Couet HG

更新日期：2002-05-01 00:00:00

abstract：:Penicillin G amidase from Providencia rettgeri is a heterodimer of 92 kDa. We have previously expressed the Pr. rettgeri pac gene coding for this enzyme in Saccharomyces cerevisiae, and now we report the expression and characterization in the methylotrophic yeast Pichia pastoris. The recombinant catalytically active e...

journal_title：FEMS yeast research

authors： Sevo M,Degrassi G,Skoko N,Venturi V,Ljubijankić G

更新日期：2002-01-01 00:00:00

abstract：:A salt-sensitive mutant of Kluyveromyces lactis was isolated that was unable to grow in high-salt media. This mutant was also respiratory-deficient and temperature-sensitive for growth. The mutation mapped in a single nuclear gene that is the ortholog of BCS1 of Saccharomyces cerevisiae. The BCS1 product is a mitochon...

journal_title：FEMS yeast research

authors： Goffrini P

更新日期：2007-03-01 00:00:00

abstract：:Saccharomyces cerevisiae grown on plastic surfaces formed organized structures, termed minicolonies, that consisted of a core of round (yeast-like) cells surrounded by chains of filamentous cells (pseudohyphae). Minicolonies had a much higher affinity for plastic than unstructured yeast communities growing on the same...

journal_title：FEMS yeast research

authors： White MG,Piccirillo S,Dusevich V,Law DJ,Kapros T,Honigberg SM

更新日期：2011-03-01 00:00:00

abstract：:Every year more than 2 million tons of citric acid (CA) are produced around the world for industrial uses. Although initially extracted from citrus, the low profitability of the process and the increasing demand soon stimulated the search for more efficient methods to produce CA. Currently, most world CA demand (99%) ...

journal_title：FEMS yeast research

authors： Cavallo E,Charreau H,Cerrutti P,Foresti ML

更新日期：2017-12-01 00:00:00

abstract：:A robust cell factory that can tolerate combined inhibitory lignocellulosic compounds is essential for the cost-effective lignocellulose-based production of second-generation bioethanol and other bulk chemicals. Following high-throughput phenotyping of a yeast genomic overexpression library, we identified a Saccharomy...

journal_title：FEMS yeast research

authors： Unrean P,Gätgens J,Klein B,Noack S,Champreda V

更新日期：2018-12-01 00:00:00

abstract：:Differences in glycosylation between the natural alpha-1,6 glucan-6-glucanohydrolase from Penicillium minioluteum and the heterologous protein expressed in the yeast Pichia pastoris were analyzed. Glycosylation profiling was carried out using fluorophore-assisted carbohydrate electrophoresis and amine absorption high-...

journal_title：FEMS yeast research

authors： Betancourt LH,García R,González J,Montesino R,Quintero O,Takao T,Shimonishi Y,Cremata JA

更新日期：2001-07-01 00:00:00

abstract：:Lignocellulosic plant biomass is the target feedstock for production of second-generation biofuels. Ionic liquid (IL) pretreatment can enhance deconstruction of lignocellulosic biomass into sugars that can be fermented to ethanol. Although biomass is typically washed following IL pretreatment, small quantities of resi...

journal_title：FEMS yeast research

authors： Sitepu IR,Shi S,Simmons BA,Singer SW,Boundy-Mills K,Simmons CW

更新日期：2014-12-01 00:00:00

abstract：:Proteostasis is a cellular housekeeping process that refers to the healthy maintenance of the cellular proteome that governs the fate of proteins from synthesis to degradation. Perturbations of proteostasis might result in protein dysfunction with consequent deleterious effects that can culminate in cell death. To dea...

journal_title：FEMS yeast research

authors： Sampaio-Marques B,Ludovico P

更新日期：2018-08-01 00:00:00

abstract：:In Saccharomyces cerevisiae, the cell integrity pathway plays a role in the oxidative stress response. In this study, we show that the Pkc1 protein mediates oxidative signalling by helping to downregulate ribosomal gene expression when cells are exposed to hydrogen peroxide. An active actin cytoskeleton is required fo...

journal_title：FEMS yeast research

authors： Mitjana FV,Petkova MI,Pujol-Carrion N,de la Torre-Ruiz MA

更新日期：2011-12-01 00:00:00

abstract：:Previous work has shown that the synthetic lethality of the slt2Δrim101Δ mutant results from a combination of factors, including improper functioning of the septum assembly machinery. Here, we identify new multicopy suppressors of this lethality including Kss1, Pcl1 and Sph1, none of which seems to be linked to the up...

journal_title：FEMS yeast research

authors： Pérez J,Arcones I,Gómez A,Casquero V,Roncero C

更新日期：2016-09-01 00:00:00

abstract：:Candida albicans maintains genetic diversity by random chromosome alterations, and this diversity allows utilization of various nutrients. Although the alterations seem to occur spontaneously, their frequencies clearly depend on environmental factors. In addition, this microorganism survives in adverse environments, w...

journal_title：FEMS yeast research

authors： Rustchenko E

更新日期：2007-01-01 00:00:00

abstract：:Many of the difficulties of studying evolution in action can be surmounted using populations of microorganisms, such as yeast. A readily manipulated sexual system and an increasingly sophisticated array of molecular and genomic tools uniquely qualify Saccharomyces cerevisiae as an experimental subject. This minireview...

journal_title：FEMS yeast research

authors： Zeyl C

更新日期：2006-08-01 00:00:00

abstract：:In the methylotrophic yeast Hansenula polymorpha non-selective autophagy, induced by nitrogen starvation, results in the turnover of cytoplasmic components, including peroxisomes. We show that the uptake of these components occurs by invagination of the vacuolar membrane without their prior sequestration and thus diff...

journal_title：FEMS yeast research

authors： Bellu AR,Kram AM,Kiel JA,Veenhuis M,van der Klei IJ

更新日期：2001-04-01 00:00:00

abstract：:This study addresses the question of whether observable changes in fluxes in the primary carbon metabolism of Saccharomyces cerevisiae occur between the different phases of the cell division cycle. To detect such changes by metabolic flux analysis, a 13C-labeling experiment was performed with a fed-batch culture inocu...

journal_title：FEMS yeast research

authors： Costenoble R,Müller D,Barl T,van Gulik WM,van Winden WA,Reuss M,Heijnen JJ

更新日期：2007-06-01 00:00:00

abstract：:Yeasts belonging to the lineage that underwent whole-genome duplication (WGD) possess a good fermentative potential and can proliferate in the absence of oxygen. In this study, we analyzed the pre-WGD yeast Kluyveromyces lactis and its ability to grow under oxygen-limited conditions. Under these conditions, K. lactis ...

journal_title：FEMS yeast research

authors： Merico A,Galafassi S,Piskur J,Compagno C

更新日期：2009-08-01 00:00:00

abstract：:Dysfunction or capacity shortage of the endoplasmic reticulum (ER) is cumulatively called ER stress and provokes the unfolded protein response (UPR). In various yeast species, the ER-located transmembrane protein Ire1 is activated upon ER stress and performs the splicing reaction of HAC1 mRNA, the mature form of which...

journal_title：FEMS yeast research

authors： Fauzee YNBM,Taniguchi N,Ishiwata-Kimata Y,Takagi H,Kimata Y

更新日期：2020-10-15 00:00:00

abstract：:Aldose-1-epimerase or mutarotase (EC 5.1.3.3) catalyzes interconversion of alpha/beta-anomers of aldoses, such as glucose and galactose, and is distributed in a wide variety of organisms from bacteria to humans. Nevertheless, the physiological role of this enzyme has been elusive in most cases, because the alpha-form ...

journal_title：FEMS yeast research

authors： Fukasawa T,Sakurai H,Nogi Y,Baruffini E

更新日期：2009-08-01 00:00:00

abstract：:Kluyveromyces marxianus is a safe yeast used in the food and biotechnology sectors. One of the important traits that sets it apart from the familiar yeasts, Saccharomyces cerevisiae, is its capacity to grow using lactose as a carbon source. Like in its close relative, Kluyveromyces lactis, this requires lactose transp...

journal_title：FEMS yeast research

authors： Varela JA,Montini N,Scully D,Van der Ploeg R,Oreb M,Boles E,Hirota J,Akada R,Hoshida H,Morrissey JP

更新日期：2017-05-01 00:00:00

abstract：:Cadmium ions disrupt reactive oxygen species/Ca(2+) homeostasis and subsequently elicit cell death and adaptive signaling cascades in eukaryotic cells. Through a functional genomics approach, we have identified deletion mutants of 106 yeast genes, including three MAP kinase genes (HOG1, SLT2, and KSS1), are sensitive ...

journal_title：FEMS yeast research

authors： Jiang L,Cao C,Zhang L,Lin W,Xia J,Xu H,Zhang Y

更新日期：2014-12-01 00:00:00