Abstract:
:This study addresses the question of whether observable changes in fluxes in the primary carbon metabolism of Saccharomyces cerevisiae occur between the different phases of the cell division cycle. To detect such changes by metabolic flux analysis, a 13C-labeling experiment was performed with a fed-batch culture inoculated with a partially synchronized cell population obtained through centrifugal elutriation. Such a culture exhibits dynamic changes in the fractions of cells in different cell cycle phases over time. The mass isotopomer distributions of free intracellular metabolites in central carbon metabolism were measured by liquid chromatography-mass spectrometry. For four time points during the culture, these distributions were used to obtain the best estimates for the metabolic fluxes. The obtained flux fits suggested that the optimally fitted split ratio for the pentose phosphate pathway changed by almost a factor of 2 up and down around a value of 0.27 during the experiment. Statistical analysis revealed that some of the fitted flux distributions for different time points were significantly different from each other, indicating that cell cycle-dependent variations in cytosolic metabolic fluxes indeed occurred.
journal_name
FEMS Yeast Resjournal_title
FEMS yeast researchauthors
Costenoble R,Müller D,Barl T,van Gulik WM,van Winden WA,Reuss M,Heijnen JJdoi
10.1111/j.1567-1364.2006.00199.xsubject
Has Abstractpub_date
2007-06-01 00:00:00pages
511-26issue
4eissn
1567-1356issn
1567-1364pii
FYR199journal_volume
7pub_type
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journal_title:FEMS yeast research
pub_type: 杂志文章
doi:10.1111/j.1567-1364.2007.00241.x
更新日期:2007-09-01 00:00:00
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journal_title:FEMS yeast research
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journal_title:FEMS yeast research
pub_type: 杂志文章
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journal_title:FEMS yeast research
pub_type: 杂志文章
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journal_title:FEMS yeast research
pub_type: 杂志文章
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journal_title:FEMS yeast research
pub_type: 杂志文章
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journal_title:FEMS yeast research
pub_type: 杂志文章
doi:10.1111/1567-1364.12043
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journal_title:FEMS yeast research
pub_type: 杂志文章
doi:10.1111/j.1567-1364.2009.00545.x
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journal_title:FEMS yeast research
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journal_title:FEMS yeast research
pub_type: 杂志文章
doi:10.1111/j.1567-1364.2008.00436.x
更新日期:2008-12-01 00:00:00
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journal_title:FEMS yeast research
pub_type: 杂志文章
doi:10.1093/femsyr/foz038
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journal_title:FEMS yeast research
pub_type: 杂志文章,评审
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更新日期:2010-12-01 00:00:00
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journal_title:FEMS yeast research
pub_type: 杂志文章
doi:10.1111/j.1567-1364.2011.00754.x
更新日期:2011-12-01 00:00:00
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journal_title:FEMS yeast research
pub_type: 杂志文章
doi:10.1093/femsyr/foy127
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journal_title:FEMS yeast research
pub_type: 杂志文章
doi:10.1093/femsyr/foy070
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journal_title:FEMS yeast research
pub_type: 杂志文章,评审
doi:10.1111/j.1567-1364.2006.00096.x
更新日期:2006-06-01 00:00:00
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journal_title:FEMS yeast research
pub_type: 杂志文章
doi:10.1111/1567-1364.12180
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journal_title:FEMS yeast research
pub_type: 杂志文章
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journal_title:FEMS yeast research
pub_type: 杂志文章
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journal_title:FEMS yeast research
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journal_title:FEMS yeast research
pub_type: 杂志文章
doi:10.1111/1567-1364.12220
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journal_title:FEMS yeast research
pub_type: 杂志文章
doi:10.1111/j.1567-1364.2009.00517.x
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journal_title:FEMS yeast research
pub_type: 杂志文章
doi:10.1093/femsyr/foz045
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journal_title:FEMS yeast research
pub_type: 杂志文章
doi:10.1111/1567-1364.12093
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journal_title:FEMS yeast research
pub_type: 杂志文章
doi:10.1016/j.femsyr.2004.12.010
更新日期:2005-05-01 00:00:00
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journal_title:FEMS yeast research
pub_type: 杂志文章,评审
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更新日期:2016-12-01 00:00:00
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journal_title:FEMS yeast research
pub_type: 杂志文章
doi:10.1111/j.1567-1364.2009.00482.x
更新日期:2009-05-01 00:00:00
abstract::The GPD1 gene encoding the glycerol-3-phosphate dehydrogenase was overexpressed in an industrial lager brewing yeast (Saccharomyces cerevisiae ssp. carlsbergensis) to reduce the content of ethanol in beer. The amount of glycerol produced by the GPD1-overexpressing yeast in fermentation experiments simulating brewing c...
journal_title:FEMS yeast research
pub_type: 杂志文章
doi:10.1111/j.1567-1364.2002.tb00087.x
更新日期:2002-05-01 00:00:00
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journal_title:FEMS yeast research
pub_type: 杂志文章
doi:10.1111/j.1567-1364.2007.00239.x
更新日期:2007-09-01 00:00:00