Old yellow enzyme interferes with Bax-induced NADPH loss and lipid peroxidation in yeast.

abstract：:Current paradigms assume that gas bubbles cannot be formed within yeasts although these workhorses of the baking and brewing industries vigorously produce and release CO(2) gas. We show that yeasts produce gas bubbles that fill a significant part of the cell. The missing link between intracellular CO(2) production b...

journal_title：FEMS yeast research

authors： Swart CW,Dithebe K,Pohl CH,Swart HC,Coetsee E,van Wyk PW,Swarts JC,Lodolo EJ,Kock JL

更新日期：2012-11-01 00:00:00

abstract：:Dysfunction or capacity shortage of the endoplasmic reticulum (ER) is cumulatively called ER stress and provokes the unfolded protein response (UPR). In various yeast species, the ER-located transmembrane protein Ire1 is activated upon ER stress and performs the splicing reaction of HAC1 mRNA, the mature form of which...

journal_title：FEMS yeast research

authors： Fauzee YNBM,Taniguchi N,Ishiwata-Kimata Y,Takagi H,Kimata Y

更新日期：2020-10-15 00:00:00

abstract：:Maintenance and adaptation of energy metabolism could play an important role in the cellular ability to respond to DNA damage. A large number of studies suggest that the sensitivity of cells to oxidants and oxidative stress depends on the activity of cellular metabolism and is dependent on the glucose concentration. I...

journal_title：FEMS yeast research

authors： Kitanovic A,Walther T,Loret MO,Holzwarth J,Kitanovic I,Bonowski F,Van Bui N,Francois JM,Wölfl S

更新日期：2009-06-01 00:00:00

abstract：:Here we present evidence that Hsp150/Pir2, a member of the Pir family of cell wall proteins, can be extracted from the purified cell walls of Saccharomyces cerevisiae by treatment with beta-mercaptoethanol, demonstrating that at least part of this protein is attached to the cell wall through disulfide bridges. We also...

journal_title：FEMS yeast research

authors： Moukadiri I,Zueco J

更新日期：2001-12-01 00:00:00

abstract：:The transport of glucose and fructose into yeast cells is a critical step in the utilization of sugars during wine fermentation. Hexose uptake can be carried out by various Hxt carriers, each possessing distinct regulatory and transport-kinetic properties capable of influencing yeast fermentation capacity. We investig...

journal_title：FEMS yeast research

authors： Perez M,Luyten K,Michel R,Riou C,Blondin B

更新日期：2005-02-01 00:00:00

abstract：:The mechanistic details of mtDNA maintenance in petite-negative yeasts have remained largely unexplored. We report here that the DNA helicase Hmi1p plays a crucial role in mtDNA stability in Candida albicans. Like its counterpart in Saccharomyces cerevisiae, Hmi1p in C. albicans (CaHmi1p) contains a C-terminal mitocho...

journal_title：FEMS yeast research

authors： Jõers P,Gerhold JM,Sedman T,Kuusk S,Sedman J

更新日期：2007-01-01 00:00:00

abstract：:The ultimate goal of therapeutic vaccines is to activate and exploit the patient's own immune system to vigorously and dynamically seek and eradicate established malignant or virally infected cells. Therapeutic vaccines also offer the potential for preventing disease recurrence. Saccharomyces cerevisiae-based vaccines...

journal_title：FEMS yeast research

authors： Ardiani A,Higgins JP,Hodge JW

更新日期：2010-12-01 00:00:00

abstract：:The yeast vacuolar membrane protein Ycf1p and its mammalian counterpart, MRP1, belong to the ABCC subfamily of ATP-binding cassette transporters. Genetic evidence suggests that the yeast casein kinase 2α, Cka1p, negatively regulates Ycf1p function via phosphorylation of Ser251 within the N-terminus. In this study, we ...

journal_title：FEMS yeast research

authors： Pickin KA,Ezenwajiaku N,Overcash H,Sethi M,Knecht MR,Paumi CM

更新日期：2010-11-01 00:00:00

abstract：:Aldose-1-epimerase or mutarotase (EC 5.1.3.3) catalyzes interconversion of alpha/beta-anomers of aldoses, such as glucose and galactose, and is distributed in a wide variety of organisms from bacteria to humans. Nevertheless, the physiological role of this enzyme has been elusive in most cases, because the alpha-form ...

journal_title：FEMS yeast research

authors： Fukasawa T,Sakurai H,Nogi Y,Baruffini E

更新日期：2009-08-01 00:00:00

abstract：:The mechanism involved in the cellular phosphate response of Saccharomyces cerevisiae forms part of the PHO pathway, which upon expression allows a co-ordinated cellular response and adaptation to changes in availability of external phosphate. Although genetic studies and analyses of the S. cerevisiae genome have prod...

journal_title：FEMS yeast research

authors： Mouillon JM,Persson BL

更新日期：2006-03-01 00:00:00

abstract：:Yeasts belonging to the lineage that underwent whole-genome duplication (WGD) possess a good fermentative potential and can proliferate in the absence of oxygen. In this study, we analyzed the pre-WGD yeast Kluyveromyces lactis and its ability to grow under oxygen-limited conditions. Under these conditions, K. lactis ...

journal_title：FEMS yeast research

authors： Merico A,Galafassi S,Piskur J,Compagno C

更新日期：2009-08-01 00:00:00

abstract：:Murine experimentation has provided many useful tools, including the ability to knockout or over-express genes and to perform experiments that are limited by ethical considerations. Over the past century, mice have imparted valuable insights into the biology of many systems, including human immunity. However, although...

journal_title：FEMS yeast research

authors： Marr KJ,Jones GJ,Mody CH

更新日期：2006-06-01 00:00:00

abstract：:In yeast, rRNA genes can be detected with the FISH technique using rRNA gene probes. This technique yields reliable, reproducible and precise results, but is time-consuming. Here, the primed in situ DNA synthesis (PRINS) procedure has been optimized for rapid detection of yeast rRNA genes. PRINS, which is as sensitive...

journal_title：FEMS yeast research

authors： Wnuk M,Lewinska A,Bugno M,Bartosz G,Slota E

更新日期：2009-06-01 00:00:00

abstract：:Kluyveromyces marxianus is a safe yeast used in the food and biotechnology sectors. One of the important traits that sets it apart from the familiar yeasts, Saccharomyces cerevisiae, is its capacity to grow using lactose as a carbon source. Like in its close relative, Kluyveromyces lactis, this requires lactose transp...

journal_title：FEMS yeast research

authors： Varela JA,Montini N,Scully D,Van der Ploeg R,Oreb M,Boles E,Hirota J,Akada R,Hoshida H,Morrissey JP

更新日期：2017-05-01 00:00:00

abstract：:The LYS12 gene from Candida albicans, coding for homoisocitrate dehydrogenase was cloned and expressed as a His-tagged protein in Escherichia coli. The purified gene product catalyzes the Mg(2+)- and K(+)-dependent oxidative decarboxylation of homoisocitrate to α-ketoadipate. The recombinant enzyme demonstrates strict...

journal_title：FEMS yeast research

authors： Gabriel I,Vetter ND,Palmer DR,Milewska MJ,Wojciechowski M,Milewski S

更新日期：2013-03-01 00:00:00

abstract：:An astaxanthin-overproducing (∼1000 μg g(-1)) strain of Phaffia rhodozyma, termed MK19, was established through 1-methyl-3-nitro-1-nitrosoguanidine and Co60 mutagenesis from wild-type JCM9042 (merely 35-67 μg g(-1)). The total fatty acid content of MK19 was much lower than that of the wild type. Possible causes of the...

journal_title：FEMS yeast research

authors： Miao L,Chi S,Tang Y,Su Z,Yin T,Guan G,Li Y

更新日期：2011-03-01 00:00:00

abstract：:Candida glabrata is a second most common human opportunistic pathogen which causes superficial but also life-threatening systemic candidosis. According to the localisation of mannans and mannoproteins in the outermost layer of the cell wall, mannan detection could be one of the first steps in the cell recognition of C...

journal_title：FEMS yeast research

authors： Paulovičová L,Paulovičová E,Farkaš P,Čížová A,Bystrický P,Jančinová V,Turánek J,Pericolini E,Gabrielli E,Vecchiarelli A,Hrubiško M

更新日期：2019-03-01 00:00:00

abstract：:The budding yeast, Saccharomyces cerevisiae, is an efficient model for studying oxidative stress, programmed cell death and aging. The present study was carried out to investigate antioxidant, the anti-apoptotic and anti-aging activity of a natural compound, astaxanthin, in S. cerevisiae model. The survivability of ye...

journal_title：FEMS yeast research

authors： Sj S,Veerabhadrappa B,Subramaniyan S,Dyavaiah M

更新日期：2019-01-01 00:00:00

abstract：:The Saccharomyces cerevisiae allantoate/ureidosuccinate permease gene (DAL5) is often used as a reporter in studies of the Tor1/2 protein kinases which are specifically inhibited by the clinically important immunosuppressant and anti-neoplastic drug, rapamycin. To date, only a single type of cis-acting element has bee...

journal_title：FEMS yeast research

authors： Rai R,Daugherty JR,Tate JJ,Buford TD,Cooper TG

更新日期：2004-10-01 00:00:00

abstract：:Development of a pulsed-light (PL) approach to inanimate surface decontamination is timely, as the incidence of yeast-related infections in healthcare remains unacceptably high. Critical electrical and biological factors governing the efficacy of PL for the in vitro inactivation of medically important yeast were estab...

journal_title：FEMS yeast research

authors： Farrell H,Garvey M,Rowan N

更新日期：2009-09-01 00:00:00

abstract：:Secreted lipases of Candida albicans are encoded by a gene family with at least 10 members (LIP1-LIP10). The expression pattern of this multigene family was investigated using reverse transcription polymerase chain reaction in experimental infections and in samples of patients suffering from oral candidosis. The findi...

journal_title：FEMS yeast research

authors： Stehr F,Felk A,Gácser A,Kretschmar M,Mähnss B,Neuber K,Hube B,Schäfer W

更新日期：2004-01-01 00:00:00

abstract：:In previous communications the essential role of spermidine in Ustilago maydis was demonstrated by means of the disruption of the genes encoding ornithine decarboxylase (ODC) and spermidine synthase (SPE). However, the assignation of specific roles to each polyamine in different cellular functions was not possible bec...

journal_title：FEMS yeast research

authors： Valdés-Santiago L,Guzmán-de-Peña D,Ruiz-Herrera J

更新日期：2010-11-01 00:00:00

abstract：:CLC-type chloride/proton antiporters are required for copper/iron homeostasis in fungi. A relationship between CLCs and Ca(2+) homeostasis has not been found before. Here we demonstrate the requirement of the antiporter CLC1 for Ca(2+) homeostasis/signaling in Cryptococcus neoformans. The deletion of CLC1 in JEC21 r...

journal_title：FEMS yeast research

authors： Li D,Zhang X,Li Z,Yang J,Pan J,Zhu X

更新日期：2012-02-01 00:00:00

abstract：:To investigate the role of the prevacuolar secretion pathway in the trafficking of vacuolar proteins in Candida albicans, the C. albicans homolog of the Saccharomyces cerevisiae vacuolar protein sorting gene VPS4 was cloned and analyzed. Candida albicans VPS4 encodes a deduced AAA-type ATPase that is 75.6% similar to ...

journal_title：FEMS yeast research

authors： Lee SA,Jones J,Khalique Z,Kot J,Alba M,Bernardo S,Seghal A,Wong B

更新日期：2007-09-01 00:00:00

abstract：:Among existing fungal pathogens, Candida glabrata is outstanding in its capacity to rapidly develop resistance to currently used antifungal agents. Resistance to the class of azoles, which are still widely used agents, varies in proportion (from 5 to 20%) depending on geographical area. Moreover, resistance to the cla...

journal_title：FEMS yeast research

authors： Vale-Silva LA,Sanglard D

更新日期：2015-06-01 00:00:00

abstract：:This study displays a screening using yeast strains deficient in protein kinases known to exist in Saccharomyces cerevisiae. From 95 viable single mutants, 20 mutants appear to be affected in the glucose-induced extracellular acidification. The mutants that are unaffected in calcium signaling were tested for their sen...

journal_title：FEMS yeast research

authors： Pereira RR,Castanheira D,Teixeira JA,Bouillet LE,Ribeiro EM,Trópia MM,Alvarez F,Correa LF,Mota BE,Conceição LE,Castro IM,Brandão RL

更新日期：2015-03-01 00:00:00

abstract：:Messenger RNAs retained in the nucleus of Saccharomyces cerevisiae are subjected to a degradation system designated DRN (Degradation of mRNA in the Nucleus) that is dependent on the nuclear mRNA cap-binding protein, Cbc1p, as well as nuclear exosome component Rrp6p, a 3' to 5' exoribonuclease. DRN has been shown to ac...

journal_title：FEMS yeast research

authors： Das S,Saha U,Das B

更新日期：2014-09-01 00:00:00

abstract：:The closely related yeasts Debaryomyces fabryi and Debaryomyces hansenii are excellent xylose consumers. We previously described the activity of a high-affinity xylose/H(+) symport from an industrial strain of D. hansenii subsequently reclassified as D. fabryi. We now report the identification of the gene encoding thi...

journal_title：FEMS yeast research

authors： Ferreira D,Nobre A,Silva ML,Faria-Oliveira F,Tulha J,Ferreira C,Lucas C

更新日期：2013-11-01 00:00:00

abstract：:The yeast Pichia anomala J121 prevents mold spoilage and enhances preservation of moist grain in malfunctioning storage systems. Development of P. anomala J121 as a biocontrol agent requires in-depth knowledge about its physiology. P. anomala J121 grew under strictly anaerobic conditions, at temperatures between 3 deg...

journal_title：FEMS yeast research

authors： Fredlund E,Druvefors U,Boysen ME,Lingsten KJ,Schnürer J

更新日期：2002-08-01 00:00:00

abstract：:Na,K-ATPase is a crucial enzyme for ion homeostasis in human tissues. Different isozymes are produced by assembly of four alpha- and three beta-subunits. The expression of the alpha3/beta1 isozyme is confined to brain and heart. Its heterologous production has so far never been attempted in a lower eukaryote. In this ...

journal_title：FEMS yeast research

authors： Reina C,Padoani G,Carotti C,Merico A,Tripodi G,Ferrari P,Popolo L

更新日期：2007-06-01 00:00:00