New aspects on phosphate sensing and signalling in Saccharomyces cerevisiae.

abstract：:In Saccharomyces cerevisiae, the cell integrity pathway plays a role in the oxidative stress response. In this study, we show that the Pkc1 protein mediates oxidative signalling by helping to downregulate ribosomal gene expression when cells are exposed to hydrogen peroxide. An active actin cytoskeleton is required fo...

journal_title：FEMS yeast research

authors： Mitjana FV,Petkova MI,Pujol-Carrion N,de la Torre-Ruiz MA

更新日期：2011-12-01 00:00:00

abstract：:The amplification by PCR of the Intergenic Spacer region (IGS) of rDNA followed by Restriction Fragment Length Polymorphism (RFLP) analysis was evaluated as a potential method for the identification of Debaryomyces hansenii among other yeast species that frequently contaminate Intermediate-Moisture Foods (IMFs). For a...

journal_title：FEMS yeast research

authors： Romero P,Patiño B,Quirós M,González-Jaén MT,Valderrama MJ,de Silóniz MI,Peinado JM

更新日期：2005-02-01 00:00:00

abstract：:This study addresses the question of whether observable changes in fluxes in the primary carbon metabolism of Saccharomyces cerevisiae occur between the different phases of the cell division cycle. To detect such changes by metabolic flux analysis, a 13C-labeling experiment was performed with a fed-batch culture inocu...

journal_title：FEMS yeast research

authors： Costenoble R,Müller D,Barl T,van Gulik WM,van Winden WA,Reuss M,Heijnen JJ

更新日期：2007-06-01 00:00:00

abstract：:Viruses are found in almost all organisms and physical habitats. One interesting example is the yeast viral 'killer system'. The virus provides the host with a toxin directed against strains that do not carry it, while the yeast cell enables its propagation. Although yeast viruses are believed to be common, they have ...

journal_title：FEMS yeast research

authors： Pieczynska MD,de Visser JA,Korona R

更新日期：2013-12-01 00:00:00

abstract：:Using modern peptide analytical MS technology ('Peptidomics'), it is possible to analyze yeast α-pheromone both qualitatively and semi-quantitatively directly from conditioned cell culture media. MS/MS analysis shows both forms of α-pheromone (MFα and MFα') detectable and identifiable straight from WT supernatants. In...

journal_title：FEMS yeast research

authors： Bener Aksam E,Pinkse MW,Verhaert PD

更新日期：2013-05-01 00:00:00

abstract：:Kluyveromyces marxianus is a safe yeast used in the food and biotechnology sectors. One of the important traits that sets it apart from the familiar yeasts, Saccharomyces cerevisiae, is its capacity to grow using lactose as a carbon source. Like in its close relative, Kluyveromyces lactis, this requires lactose transp...

journal_title：FEMS yeast research

authors： Varela JA,Montini N,Scully D,Van der Ploeg R,Oreb M,Boles E,Hirota J,Akada R,Hoshida H,Morrissey JP

更新日期：2017-05-01 00:00:00

abstract：:A biofilm-forming strain of Pichia fermentans proved to be most effective in controlling brown rot on apple fruit when coinoculated into artificial wounds with a phytopathogenic isolate of Monilinia fructicola. Culture filtrates and autoclaved cells had no significant influence on the disease. When sprayed onto the ap...

journal_title：FEMS yeast research

authors： Giobbe S,Marceddu S,Scherm B,Zara G,Mazzarello VL,Budroni M,Migheli Q

更新日期：2007-12-01 00:00:00

abstract：:Here we report on the results of a survey of the yeast populations occurring on submerged leaves (alder, eucalyptus and oak) in a natural mountain stream, during different phases of their decomposition and through two consecutive years. Leaf litter mass loss, total yeast counts, Shannon-Weiner index (H'), yeast commun...

journal_title：FEMS yeast research

authors： Sampaio A,Sampaio JP,Leão C

更新日期：2007-06-01 00:00:00

abstract：:In this work, we show that the proteins Pkc1 and Pfy1 play a role in the repolarization of the actin cytoskeleton and in cell survival in response to oxidative stress. We have also developed an assay to determine the actin polymerization capacity of total protein extracts using fluorescence recovery after photobleachi...

journal_title：FEMS yeast research

authors： Pujol N,Bonet C,Vilella F,Petkova MI,Mozo-Villarías A,de la Torre-Ruiz MA

更新日期：2009-12-01 00:00:00

abstract：:Exposure of Hansenula polymorpha cells, grown in batch cultures on methanol at 37 degrees C, to a cold treatment (18 degrees C) is paralleled by a rapid degradation of peroxisomes present in these cells. Remarkably, the events accompanying organelle degradation at 18 degrees C are similar to those of selective glucose...

journal_title：FEMS yeast research

authors： Komduur JA,Bellu AR,Knoops K,van der Klei IJ,Veenhuis M

更新日期：2004-12-01 00:00:00

abstract：:A salt-sensitive mutant of Kluyveromyces lactis was isolated that was unable to grow in high-salt media. This mutant was also respiratory-deficient and temperature-sensitive for growth. The mutation mapped in a single nuclear gene that is the ortholog of BCS1 of Saccharomyces cerevisiae. The BCS1 product is a mitochon...

journal_title：FEMS yeast research

authors： Goffrini P

更新日期：2007-03-01 00:00:00

abstract：:The feasibility of using a single vector to clone a cytochrome P450 monooxygenase (P450) in different yeasts and then compare whole-cell hydroxylase activity was investigated. A broad-range yeast expression vector using the ylTEFp to drive expression of the cloned gene and the scTEFp to drive the hygromycin resistance...

journal_title：FEMS yeast research

authors： Theron CW,Labuschagné M,Gudiminchi R,Albertyn J,Smit MS

更新日期：2014-06-01 00:00:00

abstract：:Heat shock response (HSR) is an important element of cellular homeostasis. In yeast, HSR comprises of the heat shock proteins (Hsps) and the osmolytes trehalose and glycerol. The respective roles of trehalose and Hsp104 in regulating protein aggregation remain ambiguous. We report that trehalose and Hsp104 are importa...

journal_title：FEMS yeast research

authors： Sethi R,Iyer SS,Das E,Roy I

更新日期：2018-09-01 00:00:00

abstract：:Maf1 is a repressor of RNA polymerase (Pol) III transcription for tRNA. Nutrient deprivation and environmental stress repress Pol III transcription through Maf1 in Saccharomyces cerevisiae. The sole Candida albicans homolog CaMaf1 is a protein of 380 amino acids with conserved domains and motifs of the eukaryotic Maf1...

journal_title：FEMS yeast research

authors： Asghar F,Yan H,Jiang L

更新日期：2018-09-01 00:00:00

abstract：:The yeast Zygosaccharomyces bailii, known to have peculiar resistance to several environmental constraints, is very little known with respect to its genetics and life cycle. In addition to molecular and biochemical studies, cytofluorimetric and morphological analyses can also add information necessary to shed light on...

journal_title：FEMS yeast research

authors： Dato L,Sauer M,Passolunghi S,Porro D,Branduardi P

更新日期：2008-09-01 00:00:00

abstract：:The histone acetyltransferase Sas2 is part of the SAS-I complex and acetylates lysine 16 of histone H4 (H4 K16Ac) in the genome of Saccharomyces cerevisiae. Sas2-mediated H4 K16Ac is strongest over the coding region of genes with low expression. However, it is unclear how Sas2-mediated acetylation is incorporated into...

journal_title：FEMS yeast research

authors： Reiter C,Heise F,Chung HR,Ehrenhofer-Murray AE

更新日期：2015-11-01 00:00:00

abstract：:We studied the effect of the loss of the Ser-Thr protein phosphatase Sit4, an important post-translational regulator, on the steady-state levels of the low-affinity glucose transporter Hxt1p and observed a delay in its appearance after high glucose induction, slow growth, and diminished glucose consumption. By analyzi...

journal_title：FEMS yeast research

authors： Souza AA,Miranda MN,da Silva SF,Bozaquel-Morais B,Masuda CA,Ghislain M,Montero-Lomelí M

更新日期：2012-12-01 00:00:00

abstract：:Secreted lipases of Candida albicans are encoded by a gene family with at least 10 members (LIP1-LIP10). The expression pattern of this multigene family was investigated using reverse transcription polymerase chain reaction in experimental infections and in samples of patients suffering from oral candidosis. The findi...

journal_title：FEMS yeast research

authors： Stehr F,Felk A,Gácser A,Kretschmar M,Mähnss B,Neuber K,Hube B,Schäfer W

更新日期：2004-01-01 00:00:00

abstract：:Proteostasis is a cellular housekeeping process that refers to the healthy maintenance of the cellular proteome that governs the fate of proteins from synthesis to degradation. Perturbations of proteostasis might result in protein dysfunction with consequent deleterious effects that can culminate in cell death. To dea...

journal_title：FEMS yeast research

authors： Sampaio-Marques B,Ludovico P

更新日期：2018-08-01 00:00:00

abstract：:In the macroscopic world, ecological interactions between multiple species of fauna and flora are recognised as major role-players in the evolution of any particular species. By comparison, research on ecological interactions as a driver of evolutionary adaptation in microbial ecosystems has been neglected. The evolut...

journal_title：FEMS yeast research

authors： Conacher CG,Rossouw D,Bauer FFB

更新日期：2019-11-01 00:00:00

abstract：:The rDNA sequencing data obtained during the last 5 years in several laboratories clearly demonstrate that within the heterogeneous genus Kluyveromyces there is a group of highly related species, which we refer to as the genus Zygofabospora Kudriavzev 1960 emend. G. Naumov 2002. This genus includes four hybridizing sp...

journal_title：FEMS yeast research

authors： Naumov GI,Naumova ES

更新日期：2002-03-01 00:00:00

abstract：:The budding yeast, Saccharomyces cerevisiae, is an efficient model for studying oxidative stress, programmed cell death and aging. The present study was carried out to investigate antioxidant, the anti-apoptotic and anti-aging activity of a natural compound, astaxanthin, in S. cerevisiae model. The survivability of ye...

journal_title：FEMS yeast research

authors： Sj S,Veerabhadrappa B,Subramaniyan S,Dyavaiah M

更新日期：2019-01-01 00:00:00

abstract：:Development of a pulsed-light (PL) approach to inanimate surface decontamination is timely, as the incidence of yeast-related infections in healthcare remains unacceptably high. Critical electrical and biological factors governing the efficacy of PL for the in vitro inactivation of medically important yeast were estab...

journal_title：FEMS yeast research

authors： Farrell H,Garvey M,Rowan N

更新日期：2009-09-01 00:00:00

abstract：:Ume6p is a nonessential transcription factor that represses meiotic gene expression during vegetative growth in budding yeast. To relieve this repression, Ume6p is destroyed as cells enter meiosis and is not resynthesized until spore wall assembly. The present study reveals that spores derived from a ume6 null homozyg...

journal_title：FEMS yeast research

authors： Strich R,Khakhina S,Mallory MJ

更新日期：2011-02-01 00:00:00

abstract：:Genetic relationships of 24 phenotypically different strains isolated from sorghum beer in West Africa and the type cultures of the Saccharomyces sensu stricto species were investigated by universally primed polymerase chain reaction (PCR) analysis, microsatellite fingerprinting and PCR-restriction fragment length pol...

journal_title：FEMS yeast research

authors： Naumova ES,Korshunova IV,Jespersen L,Naumov GI

更新日期：2003-04-01 00:00:00

abstract：:d-Amino acid oxidase (DAO) is an important flavo-enzyme that catalyzes the oxidative deamination of d-amino acids into the corresponding alpha-keto acid, ammonia and H(2)O(2). We identified two amino acid oxidases in the methylotrophic yeast Pichia pastoris: Dao1p, which preferentially uses d-alanine as a substrate, a...

journal_title：FEMS yeast research

authors： Klompmaker SH,Kilic A,Baerends RJ,Veenhuis M,van der Klei IJ

更新日期：2010-09-01 00:00:00

abstract：:Candida lipolytica and Candida rugosa were isolated from blood samples from a patient with chronic myeloid leukemia (31 years old) and a patient with sickle cell disease (1-year-old), respectively. Isolates were grown for 48 h at 37 degrees C in either Sabouraud or tryptone soy broth (TSB). Peptidases were tested for ...

journal_title：FEMS yeast research

authors： De Melo AC,Dornelas-Ribeiro M,De Souza EP,Macrae A,Fracalanzza SE,Vermelho AB

更新日期：2007-09-01 00:00:00

abstract：:The GPD1 gene encoding the glycerol-3-phosphate dehydrogenase was overexpressed in an industrial lager brewing yeast (Saccharomyces cerevisiae ssp. carlsbergensis) to reduce the content of ethanol in beer. The amount of glycerol produced by the GPD1-overexpressing yeast in fermentation experiments simulating brewing c...

journal_title：FEMS yeast research

authors： Nevoigt E,Pilger R,Mast-Gerlach E,Schmidt U,Freihammer S,Eschenbrenner M,Garbe L,Stahl U

更新日期：2002-05-01 00:00:00

abstract：:There is increasing evidence in the literature showing that fungal pathogens express biologically active ectoenzymes. The expression of surface phosphatases at the cell surface of Cryptococcus neoformans, the etiologic agent of cryptococcosis, was evaluated in the present study. Different isolates of C. neoformans exp...

journal_title：FEMS yeast research

authors： Collopy-Junior I,Esteves FF,Nimrichter L,Rodrigues ML,Alviano CS,Meyer-Fernandes JR

更新日期：2006-11-01 00:00:00

abstract：:Mitochondria are dynamic organelles of endosymbiotic origin that are essential components of eukaryal cells. They contain their own genetic machinery, have multicopy genomes and like their bacterial ancestors they consist of two membranes. However, the majority of the ancestral genome has been lost or transferred to t...

journal_title：FEMS yeast research

authors： Malina C,Larsson C,Nielsen J

更新日期：2018-08-01 00:00:00