Abstract:
:Using modern peptide analytical MS technology ('Peptidomics'), it is possible to analyze yeast α-pheromone both qualitatively and semi-quantitatively directly from conditioned cell culture media. MS/MS analysis shows both forms of α-pheromone (MFα and MFα') detectable and identifiable straight from WT supernatants. In addition to the mature intact α-pheromones, also post-translationally modified α-pheromone peptides and fragments thereof are found to be present in the culture medium. This molecular analytical technique is complementary to the recently described quantitation method by Rogers et al. (2012, FEMS Yeast Res. 12:668) based on ELISA.
journal_name
FEMS Yeast Resjournal_title
FEMS yeast researchauthors
Bener Aksam E,Pinkse MW,Verhaert PDdoi
10.1111/1567-1364.12032subject
Has Abstractpub_date
2013-05-01 00:00:00pages
350-3issue
3eissn
1567-1356issn
1567-1364journal_volume
13pub_type
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