The strange case of a biofilm-forming strain of Pichia fermentans, which controls Monilinia brown rot on apple but is pathogenic on peach fruit.

abstract：:Nine yeast strains were isolated from spontaneous fermentations in the Alsace area of France, during the 1997, 1998 and 1999 grape harvests. Strains were characterized by pulsed-field gel electrophoresis, PCR-restriction fragment length polymorphism (RFLP) of the MET2 gene, delta-PCR, and microsatellite patterns. Kary...

journal_title：FEMS yeast research

authors： Le Jeune C,Lollier M,Demuyter C,Erny C,Legras JL,Aigle M,Masneuf-Pomarède I

更新日期：2007-06-01 00:00:00

abstract：:Saccharomyces cerevisiae is the most widely used fermentation organism for ethanol production. However, the gene expression regulatory networks behind the ethanol fermentation are still not fully understood. Using a static fermentation model, we examined the ethanol yields on biomass of deletion mutants for 77 yeast g...

journal_title：FEMS yeast research

authors： Wu Y,Du J,Xu G,Jiang L

更新日期：2016-05-01 00:00:00

abstract：:Saccharomyces cerevisiae shows similar responses to heat shock and ethanol stress. Cells treated with severe ethanol stress activate the transcription of HSP genes and cause the aggregation of Hsp104-GFP, implying that severe ethanol stress as well as heat shock causes the accumulation of denatured proteins in yeast c...

journal_title：FEMS yeast research

authors： Kato S,Yoshida M,Izawa S

更新日期：2019-12-01 00:00:00

abstract：:Aldose-1-epimerase or mutarotase (EC 5.1.3.3) catalyzes interconversion of alpha/beta-anomers of aldoses, such as glucose and galactose, and is distributed in a wide variety of organisms from bacteria to humans. Nevertheless, the physiological role of this enzyme has been elusive in most cases, because the alpha-form ...

journal_title：FEMS yeast research

authors： Fukasawa T,Sakurai H,Nogi Y,Baruffini E

更新日期：2009-08-01 00:00:00

abstract：:Xylose fermentation is a rare trait that is immensely important to the cellulosic biofuel industry, and Candida tenuis is one of the few yeasts that has been reported with this trait. Here we report the isolation of two strains representing a candidate sister species to C. tenuis. Integrated analysis of genome sequenc...

journal_title：FEMS yeast research

authors： Haase MAB,Kominek J,Langdon QK,Kurtzman CP,Hittinger CT

更新日期：2017-05-01 00:00:00

abstract：:Maintenance and adaptation of energy metabolism could play an important role in the cellular ability to respond to DNA damage. A large number of studies suggest that the sensitivity of cells to oxidants and oxidative stress depends on the activity of cellular metabolism and is dependent on the glucose concentration. I...

journal_title：FEMS yeast research

authors： Kitanovic A,Walther T,Loret MO,Holzwarth J,Kitanovic I,Bonowski F,Van Bui N,Francois JM,Wölfl S

更新日期：2009-06-01 00:00:00

abstract：:The durum wheat TMKP1 gene encodes a MAP kinase phosphatase. When overexpressed in Saccharomyces cerevisiae, TMKP1 leads to salt stress tolerance (especially LiCl ), which is dependent on the phosphatase activity of the protein. The TMKP1-associated Li(+) resistance is restricted to a galactose-containing medium. Inte...

journal_title：FEMS yeast research

authors： Zaidi I,González A,Touzri M,Alvarez MC,Ramos J,Masmoudi K,Ariño J,Hanin M

更新日期：2012-11-01 00:00:00

abstract：:The yeast transcriptional response to murine Bax expression was compared with the changes induced by H(2)O(2) treatment via microarray technology. Although most of the Bax-responsive genes were also triggered by H(2)O(2) treatment, OYE3, ICY2, MLS1 and BTN2 were validated to have a Bax-specific transcriptional respons...

journal_title：FEMS yeast research

authors： Reekmans R,De Smet K,Chen C,Van Hummelen P,Contreras R

更新日期：2005-05-01 00:00:00

abstract：:The target of rapamycin complex 1 (TORC1) is the central controller of growth in eukaryotic cells. As one of the downstream targets of TORC1, the protein kinase ScSch9p plays multiple roles in stress resistance, longevity and nutrient sensing in Saccharomyces cerevisiae. In this study, we demonstrate that Candida albi...

journal_title：FEMS yeast research

authors： Liu W,Zhao J,Li X,Li Y,Jiang L

更新日期：2010-06-01 00:00:00

abstract：:Previous work has shown that the synthetic lethality of the slt2Δrim101Δ mutant results from a combination of factors, including improper functioning of the septum assembly machinery. Here, we identify new multicopy suppressors of this lethality including Kss1, Pcl1 and Sph1, none of which seems to be linked to the up...

journal_title：FEMS yeast research

authors： Pérez J,Arcones I,Gómez A,Casquero V,Roncero C

更新日期：2016-09-01 00:00:00

abstract：:Loss of mtDNA by the petite-negative yeast Kluyveromyces lactis is lethal (rho(o)-lethality). However, mutations in the alpha, beta and gamma subunits of F(1)-ATPase can suppress lethality by increasing intramitochondrial hydrolysis of ATP. Increased hydrolysis of ATP can also occur on inactivation of Inh1, the natura...

journal_title：FEMS yeast research

authors： Clark-Walker GD

更新日期：2007-08-01 00:00:00

abstract：:To investigate the role of the prevacuolar secretion pathway in the trafficking of vacuolar proteins in Candida albicans, the C. albicans homolog of the Saccharomyces cerevisiae vacuolar protein sorting gene VPS4 was cloned and analyzed. Candida albicans VPS4 encodes a deduced AAA-type ATPase that is 75.6% similar to ...

journal_title：FEMS yeast research

authors： Lee SA,Jones J,Khalique Z,Kot J,Alba M,Bernardo S,Seghal A,Wong B

更新日期：2007-09-01 00:00:00

abstract：:In vitro evaluation of specific anti-Candida albicans sera antibodies based on synthetically prepared complement receptor 3-related protein (CR3-RP) mimicking the structure of native complement receptor 3 in a cohort of 72 patients with atopy and recurrent Candida vulvovaginitis (RVC) revealed effective humoral respon...

journal_title：FEMS yeast research

authors： Paulovičová E,Bujdáková H,Chupáčová J,Paulovičová L,Kertys P,Hrubiško M

更新日期：2015-03-01 00:00:00

abstract：:Microorganisms have been studied and used extensively to produce value-added fuels and chemicals. Yeasts, specifically Saccharomyces cerevisiae, receive industrial attention because of their well-known ability to ferment glucose and produce ethanol. Thousands of natural or genetically modified S. cerevisiae have been ...

journal_title：FEMS yeast research

authors： Kong II,Turner TL,Kim H,Kim SR,Jin YS

更新日期：2018-02-01 00:00:00

abstract：:First-generation (1G) fuel ethanol production in sugarcane-based biorefineries is an established economic enterprise in Brazil. Second-generation (2G) fuel ethanol from lignocellulosic materials, though extensively investigated, is currently facing severe difficulties to become economically viable. Some of the challen...

journal_title：FEMS yeast research

authors： Bermejo PM,Raghavendran V,Gombert AK

更新日期：2020-06-01 00:00:00

abstract：:Genetic relationships of 24 phenotypically different strains isolated from sorghum beer in West Africa and the type cultures of the Saccharomyces sensu stricto species were investigated by universally primed polymerase chain reaction (PCR) analysis, microsatellite fingerprinting and PCR-restriction fragment length pol...

journal_title：FEMS yeast research

authors： Naumova ES,Korshunova IV,Jespersen L,Naumov GI

更新日期：2003-04-01 00:00:00

abstract：:The vast majority of wine fermentations are performed principally by Saccharomyces cerevisiae. However, there are a growing number of instances in which other species of Saccharomyces play a predominant role. Interestingly, the presence of these other yeast species generally occurs via the formation of interspecific h...

journal_title：FEMS yeast research

authors： Borneman AR,Desany BA,Riches D,Affourtit JP,Forgan AH,Pretorius IS,Egholm M,Chambers PJ

更新日期：2012-02-01 00:00:00

abstract：:Na,K-ATPase is a crucial enzyme for ion homeostasis in human tissues. Different isozymes are produced by assembly of four alpha- and three beta-subunits. The expression of the alpha3/beta1 isozyme is confined to brain and heart. Its heterologous production has so far never been attempted in a lower eukaryote. In this ...

journal_title：FEMS yeast research

authors： Reina C,Padoani G,Carotti C,Merico A,Tripodi G,Ferrari P,Popolo L

更新日期：2007-06-01 00:00:00

abstract：:A novel method dissecting the regulation of a cellular function into direct metabolic regulation and hierarchical (e.g., gene-expression) regulation is applied to yeast starved for nitrogen or carbon. Upon nitrogen starvation glucose influx is down-regulated hierarchically. Upon carbon starvation it is down-regulated ...

journal_title：FEMS yeast research

authors： Rossell S,van der Weijden CC,Kruckeberg AL,Bakker BM,Westerhoff HV

更新日期：2005-04-01 00:00:00

abstract：:Brettanomyces bruxellensis is usually considered a spoilage microorganism, responsible for significant economic losses during the production of fermented beverages such as wine, beer and cider, though for some styles of beer its influence is essential. In recent years, the competitiveness of this yeast in bioethanol p...

journal_title：FEMS yeast research

authors： Varela C,Lleixà J,Curtin C,Borneman A

更新日期：2018-11-01 00:00:00

abstract：:Genes of the post-squalene ergosterol biosynthetic pathway in Saccharomyces cerevisiae have been overexpressed in a systematic approach with the aim to construct yeast strains that produce high amounts of sterols from a squalene-accumulating strain. This strain had previously been deregulated by overexpressing a trunc...

journal_title：FEMS yeast research

authors： Veen M,Stahl U,Lang C

更新日期：2003-10-01 00:00:00

abstract：:Analysis of nucleotide sequences from the domains 1 and 2 of the large-subunit rDNA demonstrated species of the Pichia fluxuum clade to be phylogenetically isolated, and domains 1 and 2 and internal transcribed spacer rDNA sequence analyses of strains phenotypically identified as P. fluxuum resulted in the discovery o...

journal_title：FEMS yeast research

authors： Kurtzman CP

更新日期：2006-03-01 00:00:00

abstract：:Like bacteria, fungi growing in biofilms are often embedded in a so-called extracellular matrix (ECM), a complex and species-specific mixture of compounds secreted by cells in the biofilm. The precise physiological role of this ECM and its importance for the stress and drug resistance that is so characteristic of biof...

journal_title：FEMS yeast research

authors： Beauvais A,Loussert C,Prevost MC,Verstrepen K,Latgé JP

更新日期：2009-05-01 00:00:00

abstract：:The progenitor of the Dekkera/Brettanomyces clade separated from the Saccharomyces/Kluyveromyces clade over 200 million years ago. However, within both clades, several lineages developed similar physiological traits. Both Saccharomyces cerevisiae and Dekkera bruxellensis are facultative anaerobes; in the presence of e...

journal_title：FEMS yeast research

authors： Procházka E,Poláková S,Piskur J,Sulo P

更新日期：2010-08-01 00:00:00

abstract：:The amplification by PCR of the Intergenic Spacer region (IGS) of rDNA followed by Restriction Fragment Length Polymorphism (RFLP) analysis was evaluated as a potential method for the identification of Debaryomyces hansenii among other yeast species that frequently contaminate Intermediate-Moisture Foods (IMFs). For a...

journal_title：FEMS yeast research

authors： Romero P,Patiño B,Quirós M,González-Jaén MT,Valderrama MJ,de Silóniz MI,Peinado JM

更新日期：2005-02-01 00:00:00

abstract：:The expression of the transcription regulator Pdr1p and its target genes PDR5 and TPO1 is required for Saccharomyces cerevisiae adaptation and resistance to artesunate, a promising antimalarial drug, also active against tumour cells and viruses. PDR5 and TPO1 encode plasma membrane multidrug transporters of the ATP-bi...

journal_title：FEMS yeast research

authors： Alenquer M,Tenreiro S,Sá-Correia I

更新日期：2006-12-01 00:00:00

abstract：:Yeasts belonging to the lineage that underwent whole-genome duplication (WGD) possess a good fermentative potential and can proliferate in the absence of oxygen. In this study, we analyzed the pre-WGD yeast Kluyveromyces lactis and its ability to grow under oxygen-limited conditions. Under these conditions, K. lactis ...

journal_title：FEMS yeast research

authors： Merico A,Galafassi S,Piskur J,Compagno C

更新日期：2009-08-01 00:00:00

abstract：:Mitochondria are responsible for a series of metabolic functions. Superoxide leakage from the respiratory chain and the resulting cascade of reactive oxygen species-induced damage, as well as mitochondrial metabolism in programmed cell death, have been intensively studied during ageing in single-cellular and higher or...

journal_title：FEMS yeast research

authors： Breitenbach M,Rinnerthaler M,Hartl J,Stincone A,Vowinckel J,Breitenbach-Koller H,Ralser M

更新日期：2014-02-01 00:00:00

abstract：:Amylases are used in a variety of industries that have a specific need for alternative enzymes capable of hydrolysing raw starch. Five α-amylase and five glucoamylase-encoding genes were expressed in the Saccharomyces cerevisiae Y294 laboratory strain to select for recombinant strains that best hydrolysed raw corn sta...

journal_title：FEMS yeast research

authors： Cripwell RA,Rose SH,Viljoen-Bloom M,van Zyl WH

更新日期：2019-03-01 00:00:00

abstract：:Using modern peptide analytical MS technology ('Peptidomics'), it is possible to analyze yeast α-pheromone both qualitatively and semi-quantitatively directly from conditioned cell culture media. MS/MS analysis shows both forms of α-pheromone (MFα and MFα') detectable and identifiable straight from WT supernatants. In...

journal_title：FEMS yeast research

authors： Bener Aksam E,Pinkse MW,Verhaert PD

更新日期：2013-05-01 00:00:00