Characterization of a biofilm-like extracellular matrix in FLO1-expressing Saccharomyces cerevisiae cells.

Abstract:

:Like bacteria, fungi growing in biofilms are often embedded in a so-called extracellular matrix (ECM), a complex and species-specific mixture of compounds secreted by cells in the biofilm. The precise physiological role of this ECM and its importance for the stress and drug resistance that is so characteristic of biofilms remain vague. Here, we describe the discovery of an ECM produced by flocculating Saccharomyces cerevisiae cells. Although S. cerevisiae has long been believed not to produce an ECM, our results indicate that flocculating cells secrete a mixture of glucose and mannose polysaccharides that surrounds flocculating cells. This matrix impedes the penetration of large molecules into the floc, but does not seem to play a role in the resistance of flocculating cultures to drugs and ethanol. Together, our results provide a new model system to study the formation and biological role of microbial extracellular matrices.

journal_name

FEMS Yeast Res

journal_title

FEMS yeast research

authors

Beauvais A,Loussert C,Prevost MC,Verstrepen K,Latgé JP

doi

10.1111/j.1567-1364.2009.00482.x

subject

Has Abstract

pub_date

2009-05-01 00:00:00

pages

411-9

issue

3

eissn

1567-1356

issn

1567-1364

pii

FYR482

journal_volume

9

pub_type

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