Abstract:
:Like bacteria, fungi growing in biofilms are often embedded in a so-called extracellular matrix (ECM), a complex and species-specific mixture of compounds secreted by cells in the biofilm. The precise physiological role of this ECM and its importance for the stress and drug resistance that is so characteristic of biofilms remain vague. Here, we describe the discovery of an ECM produced by flocculating Saccharomyces cerevisiae cells. Although S. cerevisiae has long been believed not to produce an ECM, our results indicate that flocculating cells secrete a mixture of glucose and mannose polysaccharides that surrounds flocculating cells. This matrix impedes the penetration of large molecules into the floc, but does not seem to play a role in the resistance of flocculating cultures to drugs and ethanol. Together, our results provide a new model system to study the formation and biological role of microbial extracellular matrices.
journal_name
FEMS Yeast Resjournal_title
FEMS yeast researchauthors
Beauvais A,Loussert C,Prevost MC,Verstrepen K,Latgé JPdoi
10.1111/j.1567-1364.2009.00482.xsubject
Has Abstractpub_date
2009-05-01 00:00:00pages
411-9issue
3eissn
1567-1356issn
1567-1364pii
FYR482journal_volume
9pub_type
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