Abstract:
:Nine yeast strains were isolated from spontaneous fermentations in the Alsace area of France, during the 1997, 1998 and 1999 grape harvests. Strains were characterized by pulsed-field gel electrophoresis, PCR-restriction fragment length polymorphism (RFLP) of the MET2 gene, delta-PCR, and microsatellite patterns. Karyotypes and MET2 fragments of the nine strains corresponded to mixed chromosomal bands and restriction patterns for both Saccharomyces cerevisiae and Saccharomyces bayanus var. uvarum. They also responded positively to amplification with microsatellite primers specific to both species and were demonstrated to be diploid. However, meiosis led to absolute nonviability of their spores on complete medium. All the results demonstrated that the nine yeast strains isolated were S. cerevisiaexS. bayanus var. uvarum diploid hybrids. Moreover, microsatellite DNA analysis identified strains isolated in the same cellar as potential parents belonging to S. bayanus var. uvarum and S. cerevisiae.
journal_name
FEMS Yeast Resjournal_title
FEMS yeast researchauthors
Le Jeune C,Lollier M,Demuyter C,Erny C,Legras JL,Aigle M,Masneuf-Pomarède Idoi
10.1111/j.1567-1364.2007.00207.xsubject
Has Abstractpub_date
2007-06-01 00:00:00pages
540-9issue
4eissn
1567-1356issn
1567-1364pii
FYR207journal_volume
7pub_type
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