Abstract：
:We studied the inhibitory effects of chicken egg white ovomacroglobulin, a broad-spectrum protease inhibitor, and a synthetic protease inhibitory peptide, Bz-GFR-O-mercaptoanilide, on the growth of two strains of Serratia marcescens in a synthetic medium. The growth of the virulent protease-producing strain, S. marcescens kums 3958 (kums 3958), was more rapid than that of the strain producing minimal protease, S. marcescens kums NA (kums NA), and kums 3958 was inhibited in a dose-dependent manner by ovomacroglobulin. The synthetic inhibitor also inhibited the growth of kums 3958 weakly. Dose-dependent enhancement of growth of kums NA was observed in the medium treated with the serratial 56 kilo Dalton protease. By immunohistochemical methods using an antibody to the bacteria, the spreading of kums 3958 was also studied in corneal tissue in experimental keratitis of the guinea pig. In vivo, kums 3958 grew locally, and then spread widely by destroying stromal tissue 7-8 hours after intrastromal injection of the organisms (3 x 10(4) colony-forming units). On the other hand, when kums 3958 mixed with ovomacroglobulin was injected into the cornea, the organisms remained locally in the corneal stroma which showed a lower grade of damage. These results indicate that proteases secreted from S. marcescens destroyed corneal stroma, yielding the organisms enough space for further spreading.
journal_name
Jpn J Ophthalmoljournal_title
Japanese journal of ophthalmologyauthors
Miyagawa S,Matsumoto K,Kamata R,Okamura R,Maeda Hsubject
Has Abstractpub_date
1991-01-01 00:00:00pages
402-10issue
4eissn
0021-5155issn
1613-2246journal_volume
35pub_type
杂志文章abstract：PURPOSE:To investigate global protein expression profiles in the retinas of normal and glucocorticoid-induced ocular hypertensive rats by proteomic analysis. METHODS:Ocular hypertension was induced by topical application of dexamethasone (DEX) for 4 weeks. Age-matched untreated rats served as controls. Intraocular pre...
journal_title：Japanese journal of ophthalmology
pub_type： 杂志文章
doi：10.1007/s10384-007-0507-5
更新日期：2008-03-01 00:00:00
abstract：PURPOSE:To evaluate macular shape in normal eyes using swept-source optical coherence tomography (SS-OCT). STUDY DESIGN:Retrospective cross-sectional study. METHODS:We retrospectively evaluated 77 normal eyes of 48 subjects. Curvature of retinal pitment epithelium (RPE) and choroid/scleral interface (CSI) was measure...
journal_title：Japanese journal of ophthalmology
pub_type： 杂志文章
doi：10.1007/s10384-020-00721-8
更新日期：2020-03-01 00:00:00
abstract：:It has been reported that keratocytes endocytose foreign particles both in vitro and in vivo, suggesting the active participation of keratocytes in corneal wound healing and host defense mechanism. To understand the cellular response and the fate of engulfed foreign particles, we investigated the changes in the lysoso...
journal_title：Japanese journal of ophthalmology
pub_type： 杂志文章
doi：
更新日期：1992-01-01 00:00:00
abstract：PURPOSE:To verify the advantages of trabeculotomy combined with phacoemulsification, intraocular lens implantation and sinusotomy (PTOS) in eyes with exfoliation glaucoma (XFG) and a coexisting cataract. PATIENTS AND METHODS:Sixty-six eyes of 62 Japanese XFG patients that received either PTOS, phacotrabeculectomy (PTE...
journal_title：Japanese journal of ophthalmology
pub_type： 杂志文章
doi：10.1007/s10384-011-0024-4
更新日期：2011-05-01 00:00:00
abstract：:The ocular penetration of two beta-adrenergic blockers, befunolol and timolol, was studied using albino and pigmented rabbits. A 1% ophthalmic solution of 14C-befunolol hydrochloride or 14C-timolol maleate was instilled in one eye of nonsedated rabbits, and the concentrations of radioactive material in the ocular tiss...
journal_title：Japanese journal of ophthalmology
pub_type： 杂志文章
doi：
更新日期：1982-01-01 00:00:00
abstract：PURPOSE:From an early stage, retinitis pigmentosa (RP) patients suffer from night blindness which causes nocturnal mobility difficulties. We created a wearable visual aid that uses a high-performance see-through display, and added a high-sensitivity camera with a complementary metal-oxide-semiconductor sensor. Here, we...
journal_title：Japanese journal of ophthalmology
pub_type： 杂志文章
doi：10.1007/s10384-018-00644-5
更新日期：2019-03-01 00:00:00
abstract：:Silicone oil was injected into the eyes of 28 rabbits. Utilizing the specific affinity of osmium tetroxide for lipids, the pathological features and histochemical localization of silicone oil within the retina were studied. This osmium tetroxide technique makes it possible to identify the presence of silicone oil part...
journal_title：Japanese journal of ophthalmology
pub_type： 杂志文章
doi：
更新日期：1992-01-01 00:00:00
abstract：PURPOSE:To determine the relationship between apoptotic photoreceptor cell death and the duration of light exposure. METHODS:Ten-week-old male albino rats (Wistar strain) were dark-adapted for 2 days and then exposed to intense light for 12 hours, and 1, 2, 3, 7, 14, 21, and 28 days. The presence of apoptosis was conf...
journal_title：Japanese journal of ophthalmology
pub_type： 杂志文章
doi：10.1016/s0021-5155(99)00011-8
更新日期：1999-05-01 00:00:00
abstract：PURPOSE:To verify the current Japanese classification of vision disability in regard to visual acuity. METHODS:A questionnaire was sent to 100 ophthalmology services in Japan. Each service was asked to extract 300 of their outpatient records. From these records, patients who had a sum of corrected visual acuity in bot...
journal_title：Japanese journal of ophthalmology
pub_type： 杂志文章,多中心研究
doi：10.1007/s10384-011-0081-8
更新日期：2011-11-01 00:00:00
abstract：:We investigated the ocular fundus of (NZWXBXSB) F1 mice, which are considered to be a good model for systemic lupus erythematosus (SLE) with antiphospholipid syndrome. The abnormal fundus findings were documented by fundus photography, and the chorioretinal lesions were studied histopathologically. The incidence of oc...
journal_title：Japanese journal of ophthalmology
pub_type： 杂志文章
doi：10.1016/s0021-5155(98)00023-9
更新日期：1998-09-01 00:00:00
abstract：:An 18-year-old Japanese boy with optic disk drusen associated with left central retinal artery occlusion was reported. Transient central blurred vision, possibly caused by central retinal artery spasm, was noticed as an initial symptom of optic disk drusen in the left eye. Prolonged and repeated spasm might have given...
journal_title：Japanese journal of ophthalmology
pub_type： 杂志文章
doi：
更新日期：1982-01-01 00:00:00
abstract：:The authors experienced one case of malignant melanoma of the superior palpebral conjunctiva and the inferior palpebral skin, originating from benign acquired melanosis of the bulbar conjunctiva which developed in a 48-year-old man. Orbital exenteration including superior and inferior palpebrae was performed, and the ...
journal_title：Japanese journal of ophthalmology
pub_type： 杂志文章
doi：
更新日期：1983-01-01 00:00:00
abstract：PURPOSE:To determine whether a definite diagnosis of malignant lymphoma can be made from paraffin-embedded archived orbital specimens by gene rearrangement analysis using microdissection and polymerase chain reaction (PCR). METHODS:Specimens from four patients with histopathologically diagnosed orbital malignant lymph...
journal_title：Japanese journal of ophthalmology
pub_type： 杂志文章
doi：10.1007/s10384-003-0038-7
更新日期：2004-03-01 00:00:00
abstract：PURPOSE:To evaluate the effect of tetramethylpyrazine on the elevation of aqueous flare and intraocular pressure (IOP) induced by prostaglandin (PG) E(2) and PGE(2) receptor (EP) agonists. METHODS:PGE(2) or EP agonists (11-deoxy PGE(1), EP(2) agonist; 17-phenyl trinor PGE(2), EP(1) and EP(3) agonist; or sulprostone, E...
journal_title：Japanese journal of ophthalmology
pub_type： 杂志文章
doi：10.1016/s0021-5155(01)00320-3
更新日期：2001-05-01 00:00:00
abstract：PURPOSE:We previously reported that human conjunctival epithelial cells expressed functioning interleukin-4 receptor α (IL-4Rα). In this study, we investigated whether human corneal epithelial cells also express functioning IL-4Rα. METHODS:The presence of IL-4Rα mRNA and protein in human corneal epithelium was examine...
journal_title：Japanese journal of ophthalmology
pub_type： 杂志文章
doi：10.1007/s10384-011-0030-6
更新日期：2011-07-01 00:00:00
abstract：PURPOSE:To assess the proportion of glaucomatous eyes based on the association between optic disc (OD) size and its clinical determinants in the resident population of Namil-myon, Central South Korea. METHODS:Of the 1,532 participants in the Namil study, we included 1,410 Koreans (2,734 eyes) with legible fundus photo...
journal_title：Japanese journal of ophthalmology
pub_type： 杂志文章
doi：10.1007/s10384-014-0303-y
更新日期：2014-03-01 00:00:00
abstract：PURPOSE:To investigate cytokine mRNA expression during the inflammatory process induced in the contralateral eyes by uniocular inoculation of herpes simplex virus type 1 (HSV-1) via the anterior chamber. METHODS:BALB/c mice were inoculated in the anterior chamber with 5 x 10(4) plaque-forming units of HSV-1 (KOS). mRN...
journal_title：Japanese journal of ophthalmology
pub_type： 杂志文章
doi：10.1016/s0021-5155(02)00700-1
更新日期：2003-03-01 00:00:00
abstract：:We evaluated the clinical statistics of a total of 186 patients (101 men and 85 women) with Vogt-Koyanagi-Harada's disease seen in the Uveitis Survey Clinic of the Hokkaido University Hospital during the 18-year period from 1966 to 1983. The annual number of new cases showed some change. More new patients were seen in...
journal_title：Japanese journal of ophthalmology
pub_type： 杂志文章
doi：
更新日期：1988-01-01 00:00:00
abstract：PURPOSE:To evaluate the cycloplegic effect of mixed eye drops containing 0.5% tropicamide and 0.5% phenylephrine in myopic children, and to determine whether their efficacy was associated with their clinical characteristics. METHODS:Eighty-one myopic children (age, mean +/- SD, 11.0 +/- 1.5 years; mean spherical equiv...
journal_title：Japanese journal of ophthalmology
pub_type： 杂志文章
doi：10.1007/s10384-006-0400-7
更新日期：2007-03-01 00:00:00
abstract：:A unilateral intravitreal inoculation of herpes simplex virus type 1 (HSV-1) induced bilateral retinitis and encephalitis in the rat. Immunohistochemistry was employed to clarify the pathway of transmission of HSV-1 from the inoculated eye to the contralateral eye, and to identify localization of the viral antigen in ...
journal_title：Japanese journal of ophthalmology
pub_type： 杂志文章
doi：
更新日期：1995-01-01 00:00:00
abstract：:Full-field electroretinograms (ERGs) provide a basis for establishing the diagnosis of widespread progressive forms of retinitis pigmentosa in early life even at a time when fundus abnormalities visible with the ophthalmoscope are minimal or absent. Patients characteristically show delays in rod or cone b-wave implici...
journal_title：Japanese journal of ophthalmology
pub_type： 杂志文章,评审
doi：
更新日期：1987-01-01 00:00:00
abstract：BACKGROUND:Malassezia species rarely cause keratitis, and, thus, the clinical manifestations of Malassezia keratitis are not well known. CASE:We report the clinical findings in a 70-year-old woman who complained of pain in her left eye. Slit-lamp biomicroscopy showed a corneal ulcer with irregular infiltration, which ...
journal_title：Japanese journal of ophthalmology
pub_type： 杂志文章
doi：10.1007/s10384-007-0447-0
更新日期：2007-07-01 00:00:00
abstract：:We explored the involvement of CD44 isoforms in lymphoproliferative disorder (LPD) of the lacrimal gland of a Sjögren's syndrome patient with unilateral LPD. The CD44 variant with the v6 exon was selectively detected from infiltrating lymphocytes in the gland with LPD, but not from infiltrating lymphocytes in the norm...
journal_title：Japanese journal of ophthalmology
pub_type： 杂志文章
doi：10.1016/s0021-5155(97)00072-5
更新日期：1997-09-01 00:00:00
abstract：PURPOSE:To clarify the redox states of the aqueous humor and serum in senile cataract patients. METHODS:We analyzed the reduced and oxidized albumin fractions in 58 patients. The patients had no detectable evidence of other eye or systemic diseases and were divided into two age groups (53-75 and 76-96 years). Analysis...
journal_title：Japanese journal of ophthalmology
pub_type： 杂志文章
doi：10.1007/s10384-010-0877-y
更新日期：2010-11-01 00:00:00
abstract：PURPOSE:To measure changes in choroidal thickness (CT), retinal thickness (RT), and axial length (AL) accompanying intraocular pressure (IOP) increase and to investigate the changes in axial eye dimensions induced by IOP increase. METHODS:Thirty-four eyes of 34 patients undergoing a diagnostic provocative test for pri...
journal_title：Japanese journal of ophthalmology
pub_type： 杂志文章
doi：10.1007/s10384-012-0173-0
更新日期：2012-11-01 00:00:00
abstract：:A nationwide, multicenter study of typical retinitis pigmentosa was carried out in collaboration with 13 university hospitals throughout Japan. A total of 253 patients, 122 males and 131 females, with a wide range of ages (mean 48 years), were registered during a two-month period in 1989. Determination of inheritance ...
journal_title：Japanese journal of ophthalmology
pub_type： 杂志文章,多中心研究
doi：
更新日期：1993-01-01 00:00:00
abstract：:Astrocytes were cultured from the cerebral cortices of newborn rabbits and were so confirmed by staining of the glial fibrillary acidic protein (GFAP). In Experiment I, the astrocytes of newborn rabbits of both sexes from one mother were pooled and suspended in a culture medium, and approximately 1-2 X 10(4) cells wer...
journal_title：Japanese journal of ophthalmology
pub_type： 杂志文章
doi：
更新日期：1989-01-01 00:00:00
abstract：:Conventional vitreoretinal surgery for proliferative vitreoretinopathy is unsatisfactory despite numerous innovations over the past 20 years. Silicone oil injections in this series resulted in 70% reattachment of these end-stage cases, but within twelve months over 90% had no useful visual result. The development of a...
journal_title：Japanese journal of ophthalmology
pub_type： 杂志文章
doi：
更新日期：1987-01-01 00:00:00
abstract：:The aqueous humor during or after cataract surgery often contains red blood cells (RBCs) from blood; inflammatory cells from white blood cells (WBCs); fibrin, pigment cells, and fibroblasts from either the iris or the ciliary body; lens epithelial cells (LECs); and corneal endothelial cells. Since LECs may be related ...
journal_title：Japanese journal of ophthalmology
pub_type： 杂志文章
doi：10.1016/s0021-5155(96)00011-1
更新日期：1997-01-01 00:00:00
abstract：:The finite element algorithm is presented for the determination of heat flow within the partially frozen cornea during cryotherapy. The latent heat involved in phase change was taken into account by virtue of the enthalpy defined as the integral of heat capacity with respect to temperature. The numerical method is use...
journal_title：Japanese journal of ophthalmology
pub_type： 杂志文章
doi：
更新日期：1985-01-01 00:00:00
