Abstract：
PURPOSE:To determine whether a definite diagnosis of malignant lymphoma can be made from paraffin-embedded archived orbital specimens by gene rearrangement analysis using microdissection and polymerase chain reaction (PCR). METHODS:Specimens from four patients with histopathologically diagnosed orbital malignant lymphoma were examined. The malignant cells were microdissected off the paraffin-embedded specimens. DNA was extracted from the cells, and the immunoglobulin heavy chain ( IgH) gene was amplified by PCR. Gene rearrangements were detected by using primers for the third framework (FR3A), the second framework (FR2A), and the complementary determining region 3 (CDR3). Translocation of the B-cell lymphoma/leukemia-2 ( bcl-2) gene was also examined. RESULTS:Malignant cells were present on the slides of the paraffin-embedded specimens of three of four cases. The specimens from these three cases showed IgH rearrangements for FR3A, FR2A, and CDR3. A bcl-2-associated translocation was also detected in one case. CONCLUSIONS:Gene rearrangement analysis is applicable to paraffin-embedded archived orbital specimens to confirm a diagnosis of malignant lymphoma. The advantage of this method is that only a small specimen is needed because the detection sensitivity is high.
journal_name
Jpn J Ophthalmoljournal_title
Japanese journal of ophthalmologyauthors
Miyanaga M,Kiyosawa M,Takase H,Eishi Y,Shen DF,Chan CC,Mochizuki Mdoi
10.1007/s10384-003-0038-7keywords:
subject
Has Abstractpub_date
2004-03-01 00:00:00pages
123-7issue
2eissn
0021-5155issn
1613-2246journal_volume
48pub_type
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journal_title：Japanese journal of ophthalmology
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journal_title：Japanese journal of ophthalmology
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journal_title：Japanese journal of ophthalmology
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journal_title：Japanese journal of ophthalmology
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journal_title：Japanese journal of ophthalmology
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journal_title：Japanese journal of ophthalmology
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journal_title：Japanese journal of ophthalmology
pub_type： 杂志文章
doi：10.1016/s0021-5155(00)00270-7
更新日期：2000-11-01 00:00:00
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journal_title：Japanese journal of ophthalmology
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doi：
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journal_title：Japanese journal of ophthalmology
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doi：
更新日期：1985-01-01 00:00:00
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journal_title：Japanese journal of ophthalmology
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更新日期：2004-05-01 00:00:00
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pub_type： 杂志文章
doi：10.1007/s10384-010-0810-4
更新日期：2010-07-01 00:00:00
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journal_title：Japanese journal of ophthalmology
pub_type： 杂志文章
doi：10.1007/s10384-008-0629-4
更新日期：2009-03-01 00:00:00
abstract：:Diabetic retinopathy is a frequent cause of visual impairment in working-age adults (≥ 30 years) and in Japan is most commonly observed in those aged 50-69 years. Diabetic macular edema (DME) is one of the main causes of vision disturbance in diabetic retinopathy, which is a clinically significant microvascular compli...
journal_title：Japanese journal of ophthalmology
pub_type： 杂志文章,评审
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更新日期：2018-01-01 00:00:00
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journal_title：Japanese journal of ophthalmology
pub_type： 杂志文章
doi：10.1016/s0021-5155(00)00266-5
更新日期：2000-11-01 00:00:00
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journal_title：Japanese journal of ophthalmology
pub_type： 杂志文章
doi：10.1007/s10384-007-0481-y
更新日期：2007-11-01 00:00:00
abstract：PURPOSE:We investigated the keratocyte response and stromal remodeling after corneal incision and photorefractive keratectomy to understand the histophysiological and immunohistochemical differences between these two types of surgery. METHODS:Corneal incision or photorefractive keratectomy was performed in rabbits or ...
journal_title：Japanese journal of ophthalmology
pub_type： 杂志文章
doi：10.1016/s0021-5155(00)00269-0
更新日期：2000-11-01 00:00:00
abstract：PURPOSE:To measure choroidal thickness in healthy pregnant women using enhanced depth imaging optical coherence tomography (EDI-OCT) and compare the measurements with those of healthy non-pregnant women. METHODS:Choroidal thickness was measured at the fovea and at 3 mm superior, inferior, temporal, and nasal to the fo...
journal_title：Japanese journal of ophthalmology
pub_type： 杂志文章
doi：10.1007/s10384-013-0265-5
更新日期：2013-09-01 00:00:00
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journal_title：Japanese journal of ophthalmology
pub_type： 杂志文章
doi：10.1007/s10384-014-0320-x
更新日期：2014-07-01 00:00:00
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journal_title：Japanese journal of ophthalmology
pub_type： 杂志文章
doi：
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journal_title：Japanese journal of ophthalmology
pub_type： 杂志文章
doi：10.1007/s10384-004-0169-5
更新日期：2005-03-01 00:00:00
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journal_title：Japanese journal of ophthalmology
pub_type： 杂志文章
doi：10.1007/s10384-008-0524-z
更新日期：2008-05-01 00:00:00
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journal_title：Japanese journal of ophthalmology
pub_type： 杂志文章
doi：10.1016/s0021-5155(99)00118-5
更新日期：1999-11-01 00:00:00
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journal_title：Japanese journal of ophthalmology
pub_type： 杂志文章
doi：10.1007/s10384-018-0628-z
更新日期：2018-11-01 00:00:00
abstract：PURPOSE:To investigate various XLRS1 (RS1) gene mutations in Chinese families with X-linked juvenile retinoschisis (XLRS or RS). METHODS:Genomic DNA was isolated from leukocytes of 29 male patients with X-linked juvenile retinoschisis, 38 female carriers, and 100 normal controls. All 6 exons of the RS1 gene were ampli...
journal_title：Japanese journal of ophthalmology
pub_type： 杂志文章
doi：10.1007/s10384-007-0488-4
更新日期：2008-01-01 00:00:00
abstract：:For the past 30 years, I have worked as a physician-scientist in both the clinic and laboratory setting at a number of university medical schools. Encountering patients in the clinic for whom treatment was not available led me to the laboratory in an attempt to develop the appropriate treatment for future patients. Th...
journal_title：Japanese journal of ophthalmology
pub_type： 杂志文章,评审
doi：10.1007/s10384-012-0165-0
更新日期：2012-09-01 00:00:00
abstract：PURPOSE:To clarify the usefulness of measuring anterior chamber depth by the IOLMaster for early-stage assessment of the therapeutic effect of steroid pulse therapy in patients with Vogt-Koyanagi-Harada (VKH) syndrome with active uveitis. METHODS:Seven patients with VKH syndrome (three men and four women) participated...
journal_title：Japanese journal of ophthalmology
pub_type： 杂志文章
doi：10.1007/s10384-010-0843-8
更新日期：2010-09-01 00:00:00
abstract：PURPOSE:To evaluate aqueous flare change patterns following anti-vascular endothelial growth factor therapy for diabetic macular edema (DME) and investigate the relationship between changes in flare values and central macular thickness (CMT). STUDY DESIGN:Retrospective, interventional case series METHODS: A total of 8...
journal_title：Japanese journal of ophthalmology
pub_type： 杂志文章
doi：10.1007/s10384-019-00698-z
更新日期：2020-05-01 00:00:00
abstract：PURPOSE:Comparison of anterior chamber angle measurements using ultrasound biomicroscopy (UBM) and gonioscopy. METHODS:Five hundred subjects were evaluated for grading of angle width by the Shaffer method. UBM was done in the same group to document angle width, angle opening distance (AOD 500), and anterior chamber de...
journal_title：Japanese journal of ophthalmology
pub_type： 杂志文章
doi：10.1007/s10384-003-0004-4
更新日期：2004-01-01 00:00:00
