Abstract:
:Astrocytes were cultured from the cerebral cortices of newborn rabbits and were so confirmed by staining of the glial fibrillary acidic protein (GFAP). In Experiment I, the astrocytes of newborn rabbits of both sexes from one mother were pooled and suspended in a culture medium, and approximately 1-2 X 10(4) cells were injected into the vitreous cavity of one eye and the culture medium alone was injected into the fellow eye of the mother rabbit. In Experiment II, the astrocytes cultured from the cerebral cortex of a single newborn rabbit of known sex were injected into both eyes of the mother or father rabbit. In the 24 eyes receiving the astrocyte injection, 22 eyes developed preretinal membrane in 2.5 to 14 weeks: 11 eyes developed traction retinal detachment. In the 12 control eyes receiving the culture medium injection, preretinal membrane was seen only in one eye. The preretinal membrane consisted predominantly of spindle-shaped cells positively stained by the peroxidase-antiperoxidase method for GFAP staining. Electron microscopy showed that the major component cells of the preretinal membrane were astrocytes with characteristic intermediate filaments; no retinal pigment epithelial cells were observed. In Experiment II, the average frequency of sex chromatin detection was 4.6% and 55.2% in the male and female astrocyte cultures, respectively. Three groups of experiments were made, A) the female astrocytes injected into the mother's eyes, B) the male astrocytes injected into the mother's eyes and C) the female astrocytes injected into the father's eyes. The sex chromatin was found in the cells of the preretinal membrane at the average frequencies of 63.5%, 34.4% and 55.5% in Groups A, B and C, respectively. It was concluded that the astrocytes injected into the vitreous cavity proliferated and formed the preretinal membrane to a degree that caused traction retinal detachment, without involvement of the retinal pigment epithelial cells. It was also thought that the astrocytes from the retina of the adult host eyes participated to some extent in the preretinal membrane formation.
journal_name
Jpn J Ophthalmoljournal_title
Japanese journal of ophthalmologyauthors
Mishima N,Yamashita H,Ishii Y,Hori Ssubject
Has Abstractpub_date
1989-01-01 00:00:00pages
501-18issue
4eissn
0021-5155issn
1613-2246journal_volume
33pub_type
杂志文章abstract:PURPOSE:A national survey was conducted in Japan to analyze the clinical characteristics of pellucid marginal corneal degeneration (PMD). METHODS:A questionnaire was sent to all members of the Japan Cornea Society requesting demographic and clinical findings on PMD patients who had been examined between 2008 and 2010....
journal_title:Japanese journal of ophthalmology
pub_type: 杂志文章,多中心研究
doi:10.1007/s10384-016-0462-0
更新日期:2016-09-01 00:00:00
abstract:PURPOSE:Remodeling of the extracellular matrix occurs in the lamina cribrosa in progressed glaucomatous optic nerve damage including disc cupping. We examined immunohistochemical changes in the transforming growth factor (TGF)-beta and platelet-derived growth factor (PDGF) in the optic nerve head in an experimentally i...
journal_title:Japanese journal of ophthalmology
pub_type: 杂志文章
doi:10.1016/s0021-5155(01)00414-2
更新日期:2001-11-01 00:00:00
abstract:PURPOSE:To compare the clinical significance of the photopic negative response (PhNRs) elicited by different stimuli from glaucomatous eyes. STUDY DESIGN:Single-center observational study METHOD: Eighty-four eyes of 84 patients with open angle glaucoma (OAG) and 40 eyes of 40 normal subjects were studied. Cone electro...
journal_title:Japanese journal of ophthalmology
pub_type: 杂志文章
doi:10.1007/s10384-019-00711-5
更新日期:2020-03-01 00:00:00
abstract:PURPOSE:To compare the detection of localized retinal nerve fiber layer (RNFL) defects by two different spectral domain optical coherence tomography (SD-OCT) devices. METHODS:Eyes of 42 normal control subjects and 48 patients with a localized RNFL defect on red-free fundus photographs were imaged by the Cirrus (Carl Z...
journal_title:Japanese journal of ophthalmology
pub_type: 杂志文章
doi:10.1007/s10384-013-0239-7
更新日期:2013-07-01 00:00:00
abstract:PURPOSE:To determine whether a definite diagnosis of malignant lymphoma can be made from paraffin-embedded archived orbital specimens by gene rearrangement analysis using microdissection and polymerase chain reaction (PCR). METHODS:Specimens from four patients with histopathologically diagnosed orbital malignant lymph...
journal_title:Japanese journal of ophthalmology
pub_type: 杂志文章
doi:10.1007/s10384-003-0038-7
更新日期:2004-03-01 00:00:00
abstract:PURPOSE:To study the effect of cyclosporin (CsA) on steroid-induced cataracts in patients following renal transplantation. METHODS:The subjects comprise 140 patients who had undergone renal transplantation at Kanazawa Medical Hospital. These subjects had received ophthalmologic examinations prior to their surgery and ...
journal_title:Japanese journal of ophthalmology
pub_type: 杂志文章
doi:10.1016/s0021-5155(03)00020-0
更新日期:2003-05-01 00:00:00
abstract::Inflammation induced by systemic injection of endotoxin can be a good model for endogenous uveitis since ocular inflammation is induced without manipulating the eye. We carried out morphological and biochemical studies in Lewis rats to evaluate the breakdown of the blood-ocular barrier following injection of endotoxin...
journal_title:Japanese journal of ophthalmology
pub_type: 杂志文章
doi:
更新日期:1988-01-01 00:00:00
abstract::The clinical and etiological findings in 727 patients with viral conjunctivitis treated from January 1982 through December 1984 at Aoki Eye Clinic in Sapporo, Japan, were presented. The age of the patients ranged from 11 days to 88 years, and the monthly incidences of the disease from 18 to 131 cases, with a clusterin...
journal_title:Japanese journal of ophthalmology
pub_type: 杂志文章
doi:
更新日期:1986-01-01 00:00:00
abstract::The effects of local hypothermia on postoperative inflammation, electroretinogram (ERG) and histological changes of the retina were evaluated in experimental vitrectomy in pressure-induced ischemic rabbit eyes. The solutions used for intraocular perfusion were maintained at 8 degrees C, 22 degrees C or 38 degrees C. F...
journal_title:Japanese journal of ophthalmology
pub_type: 杂志文章
doi:
更新日期:1996-01-01 00:00:00
abstract::Prostaglandin D2 (PGD2) and 64E, a derivative of PGs, were tested for their capacity to suppress the growth of retinoblastoma in the nude mouse. Ten million cells of an established cell line of human retinoblastoma, Y-79 cells, were transferred subcutaneously into the nude mouse, and after the transferred cells became...
journal_title:Japanese journal of ophthalmology
pub_type: 杂志文章
doi:
更新日期:1987-01-01 00:00:00
abstract:PURPOSE:To determine whether the time course of average visual recovery (visual gain curve) after vitrectomy for different macular diseases can be described by a mathematical function. METHODS:The medical records of 1951 eyes that underwent vitrectomy for different macular diseases such as macular hole, epiretinal mem...
journal_title:Japanese journal of ophthalmology
pub_type: 杂志文章
doi:10.1007/s10384-010-0922-x
更新日期:2011-03-01 00:00:00
abstract::Regulation of fluid flow through corneal stroma was investigated in the bullfrog. Corneal specimens were mounted by clamping their limbal sclera between the two chambers of a Ussing-type chamber. The epithelial surface was covered with Ringer's solution, while the endothelial surface was superfused with Ringer's solut...
journal_title:Japanese journal of ophthalmology
pub_type: 杂志文章
doi:10.1016/s0021-5155(97)00111-1
更新日期:1998-01-01 00:00:00
abstract::An animal model was used to evaluate the influence of residual lens cortex on postoperative inflammation and cellular deposits on intraocular lenses (IOLs). Eighteen New Zealand white rabbits underwent bilateral endocapsular phacoemulsification with placement of a one-piece polymethylmethacrylate (PMMA) IOL in the cap...
journal_title:Japanese journal of ophthalmology
pub_type: 杂志文章
doi:
更新日期:1991-01-01 00:00:00
abstract:BACKGROUND:Although human immunodeficiency virus (HIV)-related ocular complexes are commonly observed in acquired immune deficiency syndrome (AIDS) patients, it has not been previously reported that HIV had already invaded the ocular tissues of HIV carriers. CASE:A 56-year-old woman was diagnosed as having rhegmatogen...
journal_title:Japanese journal of ophthalmology
pub_type: 杂志文章
doi:10.1016/s0021-5155(00)00278-1
更新日期:2000-11-01 00:00:00
abstract:PURPOSE:To determine the spatial distribution types of macular pigment (MP) in elderly Japanese individuals and to consider their origin. STUDY DESIGN:Observational case series. METHODS:Local MP optical density (MPOD) at some eccentricities and MP volume were measured using the MPOD module of a MultiColor Spectralis ...
journal_title:Japanese journal of ophthalmology
pub_type: 杂志文章
doi:10.1007/s10384-020-00716-5
更新日期:2020-03-01 00:00:00
abstract:PURPOSE:To establish human corneal stroma- and sclera-derived cells as models for studying diseases of the anterior segment of the eye. METHODS:Using a recombinant retrovirus system, we transfected human papilloma virus 16 E6 and E7 (HPV16 E6/E7) into human corneal stroma- and sclera-derived cells. The primary cells a...
journal_title:Japanese journal of ophthalmology
pub_type: 杂志文章
doi:10.1007/s10384-009-0749-5
更新日期:2010-01-01 00:00:00
abstract:PURPOSE:To establish with negative electroretinogram (ERG) the clinical entity of eight patients with unilateral severe photophobia, essentially normal fundus, good visual acuity, and severe cone and rod dysfunction. STUDY DESIGN:Multicenter retrospective observation case series. METHODS:Comprehensive ophthalmologic ...
journal_title:Japanese journal of ophthalmology
pub_type: 杂志文章
doi:10.1007/s10384-020-00780-x
更新日期:2020-11-12 00:00:00
abstract:PURPOSE:To compare the cytotoxicity of povidone-iodine solution (PVP-I) with that of polyvinyl alcohol-iodine solution (PAI) for ophthalmic use. METHODS:Cells of the human corneal epithelial cell line HCE-T were exposed to various dilutions of PVP-I or PAI, and the cytotoxicity of these two antiseptics was evaluated. ...
journal_title:Japanese journal of ophthalmology
pub_type: 杂志文章
doi:10.1007/s10384-014-0348-y
更新日期:2014-11-01 00:00:00
abstract::Spontaneous retinal degeneration of Celestial goldfish was studied histologically and electrophysiologically. Retinal degeneration was divided into three stages. Until the age of 75 days (Stage 0) the retina showed normal histological development and the electroretinograms (ERGs) exhibited dominant b-waves similar to ...
journal_title:Japanese journal of ophthalmology
pub_type: 杂志文章
doi:
更新日期:1987-01-01 00:00:00
abstract::The motoneurons innervating the musculus levator palpebrae superioris (LPS) of the monkey, the cat and the rabbit were studied using horseradish peroxidase (HRP). Furthermore, LPS motoneurons of the cat and rabbit were studied by the double-labeling method using two fluorescent dyes as retrograde neuronal tracers. Aft...
journal_title:Japanese journal of ophthalmology
pub_type: 杂志文章
doi:
更新日期:1995-01-01 00:00:00
abstract::Background: We report three eyes of two patients with rubella retinopathy which were associated with choroidal neovascularization in the macula.Cases: A 7-year-old girl (case 1) and a 12-year-old girl (case 2) whose mothers had suffered rubella during their pregnancy revealed typical rubella retinopathy in both eyes a...
journal_title:Japanese journal of ophthalmology
pub_type: 杂志文章
doi:10.1016/s0021-5155(00)00266-5
更新日期:2000-11-01 00:00:00
abstract:PURPOSE:To evaluate the efficacy and safety of intravitreal drug injections using a short 34-gauge needle. STUDY DESIGN:Retrospective study. METHODS:This study included patients with age-related macular degeneration, diabetic macular edema, or macular edema associated with retinal vein occlusion. We reviewed the medi...
journal_title:Japanese journal of ophthalmology
pub_type: 杂志文章
doi:10.1007/s10384-019-00663-w
更新日期:2019-05-01 00:00:00
abstract:PURPOSE:To assess intravitreal bevacizumab (IVB) for choroidal neovascularization (CNV) secondary to age-related macular degeneration (AMD) in Japanese patients. METHODS:Forty-seven patients treated with IVB for newly diagnosed subfoveal or juxtafoveal CNV (predominantly classic CNV, 15 eyes; minimally classic CNV, 11...
journal_title:Japanese journal of ophthalmology
pub_type: 杂志文章
doi:10.1007/s10384-009-0775-3
更新日期:2010-03-01 00:00:00
abstract::This is a clinical and laboratory report on 98 patients with acute viral conjunctivitis who were referred to the Eye Clinic of the Escola Paulista de Medicina of Sao Paulo, Brazil, from February 1983 to February 1984. It was planned with the objective of confirming the importance of adenovirus and enterovirus 70 as et...
journal_title:Japanese journal of ophthalmology
pub_type: 杂志文章
doi:
更新日期:1987-01-01 00:00:00
abstract::The ocular penetration of two beta-adrenergic blockers, befunolol and timolol, was studied using albino and pigmented rabbits. A 1% ophthalmic solution of 14C-befunolol hydrochloride or 14C-timolol maleate was instilled in one eye of nonsedated rabbits, and the concentrations of radioactive material in the ocular tiss...
journal_title:Japanese journal of ophthalmology
pub_type: 杂志文章
doi:
更新日期:1982-01-01 00:00:00
abstract:PURPOSE:To investigate the clinical characteristics of eyes with cuticular drusen in Japanese individuals, while paying special attention to large colloid drusen (LCD). STUDY DESIGN:Retrospective case series. METHODS:Eyes with cuticular drusen, from patients of 4 medical institutes in Japan, were investigated. Multim...
journal_title:Japanese journal of ophthalmology
pub_type: 杂志文章,多中心研究
doi:10.1007/s10384-019-00692-5
更新日期:2019-11-01 00:00:00
abstract:PURPOSE:To investigate the role of mechanical stretch in the regulation of matrix metalloproteinase-2 (MMP-2) in scleral fibroblasts of chick embryos. METHODS:Scleral fibroblasts derived from chick embryos were seeded onto flexible bottom culture plates, and subjected to a pulsatile stretch when the cells became subco...
journal_title:Japanese journal of ophthalmology
pub_type: 杂志文章
doi:10.1016/s0021-5155(01)00451-8
更新日期:2002-01-01 00:00:00
abstract::Purpose: A young patient of ophthalmoplegic migraine with recurrent oculomotor nerve palsy is reported.Case: A 4-year-old girl came to our hospital complaining of recurrent left ptosis and double vision which completely resolved within a few days. She had a history of drowsiness after vomiting every two months. Left o...
journal_title:Japanese journal of ophthalmology
pub_type: 杂志文章
doi:10.1016/s0021-5155(00)00233-1
更新日期:2000-09-01 00:00:00
abstract::A case of bilateral retinoblastoma affecting both of a pair of dizygotic twins was reported. To our knowledge, there has been only one previous report of dizygotic twins, both affected, with this disease. The present twins had no family history of retinoblastoma. They were born prematurely as extremely low birth weigh...
journal_title:Japanese journal of ophthalmology
pub_type: 杂志文章
doi:
更新日期:1992-01-01 00:00:00
abstract:PURPOSE:A prospective study was conducted to compare the effectiveness, safety, and stability of photorefractive keratectomy (PRK) and laser in situ keratomileusis (LASIK) for correction of low to moderate myopia. METHODS:Forty-five patients with a manifest refraction (PRK, -4.54 +/- 0.80; LASIK, -4.82 +/- 1.10) from ...
journal_title:Japanese journal of ophthalmology
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1016/s0021-5155(01)00406-3
更新日期:2001-09-01 00:00:00