Abstract:
:Ashwagandha, also called as "Queen of Ayurveda" and "Indian ginseng", is a commonly used plant in Indian traditional medicine, Ayurveda. Its roots have been used as herb remedy to treat a variety of ailments and to promote general wellness. However, scientific evidence to its effects is limited to only a small number of studies. We had previously identified anti-cancer activity in the leaf extract (i-Extract) of Ashwagandha and demonstrated withanone as a cancer inhibitory factor (i-Factor). In the present study, we fractionated the i-Extract to its components by silica gel column chromatography and subjected them to cell based activity analyses. We found that the cancer inhibitory leaf extract (i-Extract) has, at least, seven components that could cause cancer cell killing; i-Factor showed the highest selectivity for cancer cells and i-Factor rich Ashwagandha leaf powder was non-toxic and anti-tumorigenic in mice assays. We undertook a gene silencing and pathway analysis approach and found that i-Extract and its components kill cancer cells by at least five different pathways, viz. p53 signaling, GM-CFS signaling, death receptor signaling, apoptosis signaling and G2-M DNA damage regulation pathway. p53 signaling was most common. Visual analysis of p53 and mortalin staining pattern further revealed that i-Extract, fraction F1, fraction F4 and i-Factor caused an abrogation of mortalin-p53 interactions and reactivation of p53 function while the fractions F2, F3, F5 work through other mechanisms.
journal_name
Cancer Lettjournal_title
Cancer lettersauthors
Widodo N,Takagi Y,Shrestha BG,Ishii T,Kaul SC,Wadhwa Rdoi
10.1016/j.canlet.2007.11.037subject
Has Abstractpub_date
2008-04-08 00:00:00pages
37-47issue
1eissn
0304-3835issn
1872-7980pii
S0304-3835(07)00584-8journal_volume
262pub_type
杂志文章相关文献
CANCER LETTERS文献大全abstract::Ring1 and YY1 binding protein (RYBP) is a member of the Polycomb group (PcG) proteins and regulates cell growth through both PcG-dependent and -independent mechanisms. Our initial study indicated that RYBP is down-regulated in human non-small cell lung cancer (NSCLC) tissues. The present study determined the molecular...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/j.canlet.2015.09.003
更新日期:2015-12-28 00:00:00
abstract::Primary liver cancer is the fifth most common cancer worldwide and hepatocellular carcinoma (HCC) accounts for over 85% of all primary liver cancers. The clinical management of advanced and metastatic HCC is challenging on many counts. Besides largely occurs within a background of underlying chronic liver disease and ...
journal_title:Cancer letters
pub_type: 杂志文章,评审
doi:10.1016/j.canlet.2008.11.005
更新日期:2009-12-01 00:00:00
abstract::To determine the genotoxic risk associated with therapeutic exposure to 131I, we studied the presence of micronuclei (MN) in binucleated peripheral blood lymphocytes of a group of 22 women, patients of thyroid cancer, who received 131I sodium iodide orally as an adjuvant after total thyroidectomy. The cytogenetic stud...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/0304-3835(95)03739-j
更新日期:1995-05-08 00:00:00
abstract::Development of antiandrogen-resistance in advanced prostate cancer involves multiple androgen receptor (AR)-dependent and -independent pathways. Here, we demonstrated that endothelial nitric oxide synthase (eNOS) exhibited an overexpression pattern in hormone-refractory prostate cancer and several models of advanced h...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/j.canlet.2012.09.006
更新日期:2013-01-01 00:00:00
abstract::Hepatocellular carcinoma (HCC) is the second leading cause of cancer-related deaths worldwide. Many kinases have been found to be intimately involved in oncogenesis and the deregulation of kinase function has emerged as a major mechanism by which cancer cells evade normal physiological constraints on growth and surviv...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/j.canlet.2016.09.017
更新日期:2016-12-01 00:00:00
abstract::In order to search for the direct evidence of cellular response to low-dose radiation, we investigated wild-type p53 protein accumulation in several organs of mice after exposure to low doses of X-rays. Significant p53 accumulation within 24 h was observed in the mouse adrenal glands and pancreas after X-ray irradiati...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/0304-3835(96)04235-8
更新日期:1996-06-24 00:00:00
abstract::Intussusception is an alternative to the sprouting mode of angiogenesis. The advantage of this mechanism of vascular growth is that blood vessels are generated more rapidly and the capillaries thereby formed are less leaky. This review article summarizes our current knowledge concerning the role played by intussuscept...
journal_title:Cancer letters
pub_type: 杂志文章,评审
doi:10.1016/j.canlet.2011.10.040
更新日期:2012-03-28 00:00:00
abstract::BEC, a standard mixture of solasodine glycosides is effective in vivo against murine sarcoma 180 (S180), whereas the aglycone solasodine at equimolar concentrations is ineffective. The efficacy of BEC against S180 in vivo can be inhibited by rhamnose. Mice which are in their terminal stage with S180 can tolerate and b...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/0304-3835(90)90122-e
更新日期:1990-12-17 00:00:00
abstract::Immune checkpoint blockade (ICB) has revolutionised the treatment of solid tumours, yet most patients do not derive a clinical benefit. Resistance to ICB is often contingent on the tumour microenvironment (TME) and modulating aspects of this immunosuppressive milieu is a goal of combination treatment approaches. Radia...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/j.canlet.2020.12.045
更新日期:2021-01-12 00:00:00
abstract::One of the frontiers in cancer personalized-medicine aims at glycosylation. Cells are covered with a dense sugar coat of glycolipids, glycoproteins and free glycans. In cancer, the characteristic cell surface glycosylation is frequently transformed due to altered expression of glycan-modifying enzymes. This often lead...
journal_title:Cancer letters
pub_type: 杂志文章,评审
doi:10.1016/j.canlet.2013.10.005
更新日期:2014-09-28 00:00:00
abstract::This study focused on the role of focal adhesion kinase (FAK), a cytoplasmic tyrosine kinase important for many cellular processes, in the proliferation, adhesion, and invasion of melanoma cells in vitro and in vivo. We found that the Y925F-mutation of FAK in B16F10 melanoma cells suppressed metastasis in an experimen...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/j.canlet.2008.05.042
更新日期:2008-11-08 00:00:00
abstract:OBJECTIVE:The bad prognosis of primary carcinoma of the Fallopian tube is ascribed to early lymphogenous metastasis. Due to the rarity of cases, there exist only few and divergent results on the importance of lymph node metastasis in the relevant literature. Thus, our study aimed at detecting the incidence of lymph nod...
journal_title:Cancer letters
pub_type: 杂志文章,多中心研究
doi:10.1016/s0304-3835(99)00273-6
更新日期:1999-12-01 00:00:00
abstract::To understand the role of cyclooxygenase-2 (COX-2) in gastric cancer, we examined the abundance of COX-2, vascular endothelial growth factor-A (VEGF-A), and CD34 in 45 surgically resected human gastric cancers and paired normal gastric mucosa by immunohistochemical analysis. In addition, the message RNA (mRNA) express...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/s0304-3835(03)00153-8
更新日期:2003-05-30 00:00:00
abstract::Experimental data were provided to demonstrate the inhibitory effects of retinoids (retinal, retinoic acid, retinyl acetate and retinyl palmitate) and carotenoids (beta-carotene, lycopene and crocetin) on the growth and development of the C-6 glioma cells inoculated in rats. In the pretreatment experiments, most of th...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/0304-3835(89)90050-5
更新日期:1989-11-30 00:00:00
abstract::In the present study, the question of whether the ubiquitous environmental pollutant, 1-nitropyrene (1-NP), can induce gamma-glutamyltranspeptidase (GGT)-positive foci, an early lesion occurring during hepatocarcinogenesis, when given orally to F344 rats was examined. Significant induction of GGT-positive foci was obs...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/0304-3835(89)90031-1
更新日期:1989-04-01 00:00:00
abstract::Interleukin-2 (IL-2) and granulocyte-macrophage colony stimulating factor (GM-CSF) facilitate the maturation and functioning of injected DC. We developed a method of in situ electroporation using IL-2 and GM-CSF genes (EGT/cytokines), followed by intra-tumoral (i.t.) immature DC to determine the immune response at the...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/j.canlet.2008.02.063
更新日期:2008-08-08 00:00:00
abstract::The effect of the naturally occurring flavonol, quercetin, was investigated on cell growth and metabolism of two human carcinoma cell lines, HT29 and Caco-2 cells, both during the exponentially growing phase and after confluence. Our results show clearly that, after a 48-h period of treatment, quercetin (in the range ...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/0304-3835(94)90409-x
更新日期:1994-11-25 00:00:00
abstract::To be capable of selective killing of tumor cells, the non-selective Pseudomonas aeruginosa exotoxin A must have its cell-binding domain inactivated or removed and then be chemically linked to, or genetically fused with, a specific targeting agent. In the present study, epsilon-NH2 groups of lysine residues of the cel...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/0304-3835(90)90241-o
更新日期:1990-04-20 00:00:00
abstract::The anticarcinogenic action of the garlic constituent diallyl sulfide (DAS), was examined in the hamster buccal pouch and forestomach. Groups of hamsters were topically treated, for up to 14 weeks, with a 0.5% solution of the buccal pouch and forestomach carcinogen 7,12-dimethylbenz[a]anthracene (DMBA). Prior to, duri...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/0304-3835(92)90249-u
更新日期:1992-10-21 00:00:00
abstract::The notions of inter- and intra-tumour heterogeneity (ITH) have been recognised for many years but recent advances in sequencing technology are allowing the true extent of both forms of heterogeneity to be revealed in detail for the first time. In this review we examine the current evidence for ITH, the possibility of...
journal_title:Cancer letters
pub_type: 杂志文章,评审
doi:10.1016/j.canlet.2012.10.031
更新日期:2013-11-01 00:00:00
abstract::The small 25 kDa peptide, neutrophil gelatinase-associated lipocalin (NGAL), first known as an antibacterial factor of natural immunity, and an acute phase protein, is currently one of the most interesting and enigmatic proteins involved in the process of tumor development. The aim of the present review is to point ou...
journal_title:Cancer letters
pub_type: 杂志文章,评审
doi:10.1016/j.canlet.2009.05.027
更新日期:2010-02-01 00:00:00
abstract::We previously showed that polyphyllin D (PD) produced a stronger apoptotic effect in R-HepG2 with multi-drug resistance (MDR) than that in its parent HepG2 cells without MDR. In this study, PD was found to elicit mitochondrial fragmentation in live cells by using total internal reflection fluorescence microscopy (TIRF...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/j.canlet.2007.11.005
更新日期:2008-03-18 00:00:00
abstract::Inflammasomes are large multimolecular complexes present in the cytosol of stimulated immune cells; they mediate the activation of caspase-1, leading to cellular pyroptosis. So far, a variety of studies on inflammasomes have emerged, and the best-studied is the NLRP3 inflammasome that is involved in many inflammatory ...
journal_title:Cancer letters
pub_type: 杂志文章,评审
doi:10.1016/j.canlet.2018.01.034
更新日期:2018-04-10 00:00:00
abstract::The intracellular androgen metabolism and cell activity in prostate cancer cells with mutated (LNCaP-FGC) or wild-type (VCaP) androgen receptors in the presence of trilostane, an inhibitor of 3β-hydroxysteroid dehydrogenase, were examined. Trilostane suppressed the intracellular production of androstenedione, testoste...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/j.canlet.2010.05.015
更新日期:2010-11-28 00:00:00
abstract::The carcinogenic effect of ultraviolet radiation (UVR) on the skin of hairless (hr) mice was modified by two immunosuppressive agents, rabbit anti-mouse lymphocytic serum (ALS), and 6-mercaptopurine (6MP). Daily exposure of mice to UVR resulted in multiple tumor production. Carcinogenesis was measured in terms of affe...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/s0304-3835(75)97305-x
更新日期:1976-05-01 00:00:00
abstract::The accumulation of benzoporphyrin derivative (BPD) in malignant and host cell populations of the mouse RIF tumor was studied by flow cytometry analysis of cells dissociated from the tumor tissue. The highest cellular BPD levels were found in a subpopulation of tumor associated macrophages (TAMs) characterized by a hi...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/0304-3835(95)03985-6
更新日期:1995-11-06 00:00:00
abstract::The activity of cancer procoagulant (CP), prothrombin time (PT), activated partial thromboplastin time (APTT), the concentration of thrombin-antithrombin complexes (TAT) and the concentration of fibrinogen were analysed in blood of breast cancer patients scheduled for surgery. The serum level of CP activity was depend...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/s0304-3835(99)00230-x
更新日期:1999-11-01 00:00:00
abstract::The regulator of G protein signaling 10 (RGS10) protein is a GTPase activating protein that accelerates the hydrolysis of GTP and therefore canonically inactivates G proteins, ultimately terminating signaling. Rheb is a small GTPase protein that shuttles between its GDP- and GTP-bound forms to activate mTOR. Since RGS...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/j.canlet.2015.08.012
更新日期:2015-12-01 00:00:00
abstract::Human RecQ helicases that share homology with E. coli RecQ helicase play critical roles in diverse biological activities such as DNA replication, transcription, recombination and repair. Mutations in three of the five human RecQ helicases (RecQ1, WRN, BLM, RecQL4 and RecQ5) result in autosomal recessive syndromes char...
journal_title:Cancer letters
pub_type: 杂志文章,评审
doi:10.1016/j.canlet.2017.10.021
更新日期:2018-01-28 00:00:00
abstract::Cytokines induce inflammatory and immune responses in tumors after photodynamic therapy (PDT). Since there are no reports of IL-6 in nasopharyngeal cancer (NPC) cells following PDT, we evaluated IL-6 expression in two different NPC tumors after hypericin mediated PDT. Interleukin-6 transcription was significantly upre...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/j.canlet.2005.04.013
更新日期:2006-04-28 00:00:00