Abstract:
:To be capable of selective killing of tumor cells, the non-selective Pseudomonas aeruginosa exotoxin A must have its cell-binding domain inactivated or removed and then be chemically linked to, or genetically fused with, a specific targeting agent. In the present study, epsilon-NH2 groups of lysine residues of the cell-binding domain of exotoxin A were extensively propionylated with N-succinimidyl-3-propionate (NSP). The NSP-treated exotoxin retained its cytocidal ADP-ribosyltransferase activity, but it could no longer bind to, and inhibit the proliferation of, Friend murine erythroleukemia cells. Cytotoxicity (i.e., the ability to inhibit proliferation) for the Friend erythroid cells was restored completely to the NSP-inactivated exotoxin by conjugating it to ADIF, an autocrine factor secreted by chicken erythroleukemia cells which selectively inhibits the differentiation of erythroid cells such as Friend erythroleukemia cells without inhibiting their proliferation.
journal_name
Cancer Lettjournal_title
Cancer lettersauthors
Bourdenet S,Doyonnas R,Vacheron MJ,Guinand M,Fasciotto B,Ristic A,Michel G,Cozzone AJ,Durkin JP,Whitfield JFdoi
10.1016/0304-3835(90)90241-osubject
Has Abstract,Author List Incompletepub_date
1990-04-20 00:00:00pages
121-7issue
2eissn
0304-3835issn
1872-7980pii
0304-3835(90)90241-Ojournal_volume
50pub_type
杂志文章相关文献
CANCER LETTERS文献大全abstract::A single administration of enantiomerically pure 11,12-dihydrodiols of dibenzo[a,l]pyrene (DB[a,l]P) on the back of NMRI mice and subsequent chronic treatment with 12-O-tetradecanoylphorbol 13-acetate (TPA) (initiation/promotion assay) revealed strikingly different carcinogenic activities of both enantiomers. Tumor-in...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/s0304-3835(98)00265-1
更新日期:1999-03-01 00:00:00
abstract::Previously, we have shown that simian virus 40 (SV40) T antigen can directly cause apoptosis in immortalized human epithelial cells under normal growth conditions. In this study, we further characterized the mechanism of T-antigen-mediated apoptosis involving p53 and whether T antigen can suppress erbB2/neu gene expre...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/s0304-3835(98)00360-7
更新日期:1999-03-22 00:00:00
abstract::In order to discover the role in the promotion process of 5-lipoxygenase (5-LO), a key enzyme in the arachidonate cascade, the antipromoting effects of the 5-LO inhibiting 3-nitro-2,4,6-trihydroxybenzamide (NTB) derivatives were studied in a two-stage transformation assay system using BALB 3T3 cells. All compounds inh...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/0304-3835(93)90097-s
更新日期:1993-07-30 00:00:00
abstract::Rafoxanide is used in veterinary medicine for the treatment of fascioliasis. We previously repositioned the drug as the inhibitor of B-Raf V600E, but its anti-tumor effect in human cancer has never been reported. In this study, we investigated the effects of rafoxanide in multiple myeloma (MM) in vitro and in vivo. We...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/j.canlet.2018.12.014
更新日期:2019-03-01 00:00:00
abstract::The uptake of several metallophthalocyanine tetrasulfonates by cultured Chinese hamster cells was studied. Uranyl- and chloraluminium were taken up at the highest rate followed by Ni-, Zn-, Cu-, Co- and dichlorosilicon-phthalocyanine tetrasulfonate. The uptake from the growth medium containing 10% serum in which only ...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/0304-3835(87)90217-5
更新日期:1987-12-01 00:00:00
abstract::Hypoxia-inducible factor 1 (HIF-1) is a heterodimeric transcription factor composed of alpha and beta subunits, which plays an essential role in cancer cell hypoxia adaptation, glycolysis, and angiogenesis. Normally, HIF-1alpha protein, the dominant subunit of HIF-1, is accumulated in nuclei when cells are exposed to ...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/s0304-3835(02)00053-8
更新日期:2002-07-26 00:00:00
abstract::The in vitro induction of sister chromatid exchanges (SCEs) in Chinese hamster ovary (CHO) cells by the polycyclic hydrocarbons, 7,12-dimethylbena[a]anthracene and 3-methylcholanthrene and some of the related dihydrodiols was investigated. Increased numbers of SCEs were seen in the chromosomes of cells exposed to non-...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/s0304-3835(79)80075-0
更新日期:1979-06-01 00:00:00
abstract::The suitability of a colorimetric [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT)] assay to the determination of cell viability following photoradiation therapy (PRT) of human breast and melanoma cell lines has been examined. Results have been shown to correlate with those obtained using a clonogen...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/0304-3835(88)90293-5
更新日期:1988-08-30 00:00:00
abstract::We generated three anti-ErbB-2 monoclonal antibodies (mAbs) by using surface-epitope masking method (SEM method). They were characterized and shown to recognize the extracellular domain (ECD) of ErbB-2 specifically. In order to detect ErbB-2 in the sera of breast cancer patients, we used two of them: A18 and A21 to de...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/s0304-3835(03)00017-x
更新日期:2003-04-25 00:00:00
abstract::A newly found human retrovirus, adult T-cell leukemia virus (ATLV) was shown by means of membrane immunofluorescence to bind to various hematopoietic cells including T-, B- and non-T, non-B-cell lines. Partially purified viral gp46 from culture fluids of ATL virus producer lines also bound efficiently to an ATLV-negat...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/0304-3835(84)90004-1
更新日期:1984-01-01 00:00:00
abstract::Samples of human endometrial carcinomas of ranging degree of histological differentiation, and normal endometrial tissues were screened for the presence of single site DNA mutations at codon 12 of the C-K-ras gene using oligonucleotide hybridisation of DNA amplified by the polymerase chain reaction (PCR). Two instance...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/0304-3835(90)90223-k
更新日期:1990-05-15 00:00:00
abstract::The p53 tumor suppressor gene is often mutated in various human cancers. Recently, the p53 codon 72 polymorphism has been extensively studied to determine the risk factors responsible for cancer formation. We investigated the genotype distribution of the p53 codon 72 polymorphism in 112 male urothelial cancer cases an...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/s0304-3835(02)00518-9
更新日期:2003-01-10 00:00:00
abstract::The aim of the study was to demonstrate the role of parathyroid hormone related protein (PTHrP) in stimulating aldo-keto reductase (AKR) 1C3 expression in prostate cancer (CaP) cells. CaP cell proliferation and resistance to apoptosis was increased by PTHrP transfection. Conversely, reducing AKR1C3 expression by siRNA...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/j.canlet.2011.02.027
更新日期:2011-07-01 00:00:00
abstract::In situ markers for quantitative analysis of cell proliferation and apoptotic cell death have been used to evaluate both the proliferation level and net growth potential of preneoplastic nodules and malignant tumor tissues from rats experimentally induced for hepatocarcinogenesis by the dietary administration of 2-ace...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/0304-3835(96)04291-7
更新日期:1996-08-02 00:00:00
abstract::Human kinome/phosphatome screen identified CAMK2N1 genes suppressing the development of human hepatocellular carcinoma (HCC). CAMK2N1 downregulation was found in 47% HCCs and associated with poor prognosis. The downregulation was mainly attributed to its genome deletion (28.4%) and DNA hypermethylation of its promoter...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/j.canlet.2020.10.017
更新日期:2021-01-28 00:00:00
abstract::Effects of cis-dichlorodiammineplatinum-(II) (cis-DDP), an anticancer agent, on capping of membrane receptors were investigated in mouse spleen cells in vitro, using fluorescein labeled ligands. Cap formations of surface immunoglobulin and of concanavalin A receptors were significantly reduced by cis-DDP in relation t...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/0304-3835(80)90079-8
更新日期:1980-09-01 00:00:00
abstract::Herein we report a method for studying slow acting pharmaceutical COX-2 inhibitors in the MCF-7 breast cancer cell line where cells are grown in a three-dimensional format within an agarose matrix. The cancer cells are suspended in agarose and plated in a cell culture dish, where they will form small multicellular 'tu...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/j.canlet.2003.10.030
更新日期:2004-03-08 00:00:00
abstract::Transforming growth factors (TGFs) are implicated in malignancy, therefore qualitative and quantitative differences of these growth factors in chronic myelogenous leukemia (CML) in chronic phase has been investigated in this report. Induction of anchorage independent growth of BALB/c 3T3 and NRK-49F fibroblasts was us...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/0304-3835(86)90181-3
更新日期:1986-09-01 00:00:00
abstract::Experiments were conducted to determine whether gamma-ray-induced genetic damage in parental rats can lead to the development of cancer in their offspring rats using glutathione S-transferase-positive (GST-P+) hepatic foci with or without the addition of diethylnitrosamine (DEN), a carcinogen. A single 1 Gy whole-body...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/s0304-3835(98)00155-4
更新日期:1998-10-23 00:00:00
abstract::Fludarabine phosphate is a synthetic analog of beta-arabinofuranosyl adenine (beta-ara-A), an anti-viral agent. Since beta-ara-A has been shown to be an effective inhibitor of potentially lethal damage (PLD) repair in cell culture system but ineffective in in vivo tumors, we carried out experiments to determine whethe...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/0304-3835(86)90168-0
更新日期:1986-04-01 00:00:00
abstract::Estrogens are important for the development and growth of estrogen receptor (ER)-positive breast cancer, for which anti-estrogen therapy is one of the most effective treatments. However, its efficacy can be limited by either de novo or acquired resistance. Aromatase is a key enzyme for the biosynthesis of estrogens, a...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/j.canlet.2014.05.003
更新日期:2014-08-28 00:00:00
abstract::Telomerase activity (TA) and telomerase reverse transcriptase (rTERT) were investigated in Sprague-Dawley rats, killed at 6 and 12 h, and 1, 2, 3, 7, and 14 days after 90% pancreatectomy (px), by TRAPeze enzyme-linked immunosorbent assay telomerase detection and reverse transcriptase-polymerase chain reaction. TA incr...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/s0304-3835(02)00320-8
更新日期:2002-12-01 00:00:00
abstract::The majority of ovarian cancer (OC) patients recur with a platinum-resistant disease. OC cells activate adaptive resistance mechanisms that are only partially described. Here we show that OC cells can adapt to chemotherapy through a positive-feedback loop that favors chemoresistance. In platinum-resistant OC cells we ...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/j.canlet.2020.08.026
更新日期:2020-11-01 00:00:00
abstract::We evaluated the association between the XPD exon 10 Asp(312)Asn and exon 23 Lys(751)Gln polymorphisms and the risk of pancreatic cancer in a hospital-based study of 344 patients and 386 controls frequency matched by age, gender, and race. Stratified analyses showed ever smokers carrying the Asn(312)Asn genotype had a...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/j.canlet.2005.12.026
更新日期:2007-01-08 00:00:00
abstract::Neuroblastoma (NB) is a solid childhood tumour that exhibits heterogeneous biological and clinical phenotypes. Multiple drug resistance marks a major complication especially in high-risk patients with advanced tumour stages and specific genetic aberrations, such as MYCN amplification and lp deletion. As an approach to...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/j.canlet.2006.09.010
更新日期:2007-05-18 00:00:00
abstract::NF-kappaB transcription factor regulates important cellular processes ranging from establishment of the immune and inflammatory responses to regulation of cell proliferation or apoptosis, through the induction of a large array of target genes. NF-kappaB is now considered as an important actor in the tumorigenic proces...
journal_title:Cancer letters
pub_type: 杂志文章,评审
doi:10.1016/j.canlet.2005.01.022
更新日期:2006-01-18 00:00:00
abstract::Pancreatic cancer has a poor prognosis, even with surgery. ING4 is a member of the inhibitor of growth (ING) tumor suppressor family that has potent inhibitory effects on a variety of tumors; meanwhile, radiotherapy is a common adjunctive therapy for pancreatic cancer. Prior to this study, the effectiveness of a combi...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/j.canlet.2011.11.003
更新日期:2012-03-28 00:00:00
abstract::Triple-negative breast cancer (TNBC) is the most difficult subtype of breast cancer to treat due to a paucity of effective targeted therapies. Many studies have reported that breast cancer stem cells (BCSCs) are enriched in TNBC and are responsible for chemoresistance and metastasis. In this study, we identify LRP8 as...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/j.canlet.2018.09.022
更新日期:2018-12-01 00:00:00
abstract::Vascular recovery or re-angiogenesis after radiotherapy plays a significant role in tumor recurrence, whereas molecular mechanisms of this process remain elusive. In this work, we found that dying glioma cells promoted post-irradiation angiogenesis through a caspase 3 dependent mechanism. Evidence in vitro and in vivo...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/j.canlet.2016.10.042
更新日期:2017-01-28 00:00:00
abstract::Epidermal growth factor receptor (EGFR) is highly expressed in many human tumors including hepatocellular carcinoma (HCC). Therefore, inhibition of EGF receptors could be a potential target for anticancer therapy. In this study, we investigated the effects of two EGFR tyrosine kinase inhibitors, PD153035 and its analo...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/j.canlet.2006.05.018
更新日期:2007-04-08 00:00:00