Abstract:
:We have performed proteomic analysis in the cerebrospinal fluid in an animal model of Parkinson's disease induced by axotomy of the medial forebrain bundle. In this model, the degeneration of dopaminergic neurons was completed in 14 days, with a loss of about 50% dopaminergic neurons in the substantia nigra and a loss of more than 80% dopamine terminals in the striatum, with a similar diminution of dopamine levels in both structures. Proteins were separated by 2D electrophoresis and identified by matrix-assisted laser desorption-ionization time-of-flight (MALDI-TOF). We found significant increases of haptoglobin and transthyretin along with a decrease of Apo E concentrations in the cerebrospinal fluid of axotomized animals. Changes for haptoglobin and transthyretin were further confirmed in cerebrospinal fluid and plasma by Western blotting. These results suggest that monitoring plasma levels of these signals appears to be a promising biological marker of neuronal degeneration of the nigrostriatal dopaminergic system.
journal_name
J Neurosci Resjournal_title
Journal of neuroscience researchauthors
Rite I,Argüelles S,Venero JL,García-Rodriguez S,Ayala A,Cano J,Machado Adoi
10.1002/jnr.21452subject
Has Abstractpub_date
2007-12-01 00:00:00pages
3607-18issue
16eissn
0360-4012issn
1097-4547journal_volume
85pub_type
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